• Title/Summary/Keyword: AUX1

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Isolation and characterization of Auxin/indole-3-acetic acid 1 (Aux/IAA1) gene from poplar (Populus alba × P. glandulosa) (현사시나무에서 Auxin/indole-3-acetic acid 1 (Aux/IAA1) 유전자 분리 및 발현 특성 구명)

  • Bae, Eun-Kyung;Choi, Young-Im;Lee, Hyoshin;Choi, Ji Won
    • Journal of Plant Biotechnology
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    • v.46 no.3
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    • pp.180-188
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    • 2019
  • Auxin plays a crucial regulatory role in plant growth and development processes. Three major classes of auxin-responsive transcription factors controlled by the Auxin/indole-3-acetic acid (Aux/IAA), Gretchen Hagen 3 (GH3), and small auxin up RNA (SAUR) genes regulate auxin signaling. Aux/IAA, in particular, encodes short-lived nuclear proteins that accumulate rapidly in response to auxin signaling. In this study, we isolated a PagAux/IAA1 gene from poplar (Populus alba ${\times}$ P. glandulosa) and investigated its expression characteristics. The PagAux/IAA1 cDNA codes for putative 200 amino acids polypeptide containing four conserved domains and two nuclear localization signals (NLSs). Utilizing Southern blot analysis, we confirmed that a single copy of the PagAux/IAA1 gene was present in the poplar genome. The expression of this gene is specific to leaves and flowers of the poplar. PagAux/IAA1 expressed in the early exponential growth phase of cell-cultured in suspension. PagAux/IAA1 expression level reduced in drought and salt stress conditions, and the presence of plant hormones such as abscisic acid. However, expression enhanced in cold stress, cambial cell division, and presence of plant hormones such as gibberellic acid and jasmonic acid. Thus, these results suggest that PagAux/IAA1 participates in cold stress response as well as developmental processes in the poplar.

A Design of DisplayPort AUX Channel (디스플레이포트 인터페이스의 AUX 채널 설계)

  • Cha, Seong-Bok;Yoon, Kwang-Hee;Kim, Tae-Ho;Kang, Jin-Ku
    • Journal of IKEEE
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    • v.14 no.1
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    • pp.1-7
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    • 2010
  • This paper presents an implementation of the DisplayPort AUX(Auxiliary) Channel. DisplayPort uses Main link, AUX Channel and Hot Plug Detect line to transfer the video & audio data. For isochronous transport service, source device converts to image and audio data which are to be transported through the Main Link and transports the restructured image and audio data to sink device. The AUX Channel provides link service and device service for discovering, initializing and maintaining the Main link. Hot Plug Detect line is used to confirm the connection between source device and sink device. The AUX Channel is implemented with 3315 LUTs(Look Up Table), 1466 Flip Flops and 168.782MHz max speed synthesized using Xilinx ISE 9.2i at SoC Master3.

Auxin Induced Expression of Expansin is Alered in a New Aux1 Allele that Shows Severe Defect in Gravitropic Response

  • Jeong, Hae-Jun;Kwon, Ye-Rim;Oh, Jee-Eun;Kim, Ki-Deok;Lee, Sung-Joon;Hong, Suk-Whan;Lee, Ho-Joung
    • Journal of Applied Biological Chemistry
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    • v.49 no.4
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    • pp.148-153
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    • 2006
  • While the underlying molecular mechanism remains to be elucidated, recent studies suggest that polar auxin transport is a key controlling factor in triggering differential growth responses to gravity. Identification of regulatory components in auxin-mediated differential cell expansion would improve our understanding of the gravitropic response. In this study, we identify a mutant designated aux1-like(later changed to aux1), an allele of the aux1 mutant that exhibits a severely disrupted root gravitropic response, but no defects in developmental processes. In Arabidopsis, AUX1 encodes an auxin influx carrier. Since in-depth characterization of the gravitropic response caused by mutations in this gene has been performed previously, we focused on identifying the downstream genes that were differentially expressed compared to wild-type plants. Consistent with the mutant phenotype, the transcription of the auxin-responsive genes IAA17 and GH3 were altered in aux1 plants treated with IAA, 2, 4-D and NAA. In addition, we identified two expansin genes EXP10 and EXPL3 that exhibited different expression in wild-type and mutant plants.

Application of data fusion modeling for the prediction of auxin response elements in Zea mays for food security purposes

  • Nesrine Sghaier;Rayda Ben Ayed;Ahmed Rebai
    • Genomics & Informatics
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    • v.20 no.4
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    • pp.45.1-45.7
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    • 2022
  • Food security will be affected by climate change worldwide, particularly in the developing world, where the most important food products originate from plants. Plants are often exposed to environmental stresses that may affect their growth, development, yield, and food quality. Auxin is a hormone that plays a critical role in improving plants' tolerance of environmental conditions. Auxin controls the expression of many stress-responsive genes in plants by interacting with specific cis-regulatory elements called auxin-responsive elements (AuxREs). In this work, we performed an in silico prediction of AuxREs in promoters of five auxin-responsive genes in Zea mays. We applied a data fusion approach based on the combined use of Dempster-Shafer evidence theory and fuzzy sets. Auxin has a direct impact on cell membrane proteins. The short-term auxin response may be represented by the regulation of transmembrane gene expression. The detection of an AuxRE in the promoter of prolyl oligopeptidase (POP) in Z. mays and the 3-fold overexpression of this gene under auxin treatment for 30 min indicated the role of POP in maize auxin response. POP is regulated by auxin to perform stress adaptation. In addition, the detection of two AuxRE TGTCTC motifs in the upstream sequence of the bx1 gene suggests that bx1 can be regulated by auxin. Auxin may also be involved in the regulation of dehydration-responsive element-binding and some members of the protein kinase superfamily.

APOLLO2 YEAR 2010

  • Sanchez, Richard;Zmijarevi, Igor;Coste-Delclaux, M.;Masiello, Emiliano;Santandrea, Simone;Martinolli, Emanuele;Villate, Laurence;Schwartz, Nadine;Guler, Nathalie
    • Nuclear Engineering and Technology
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    • v.42 no.5
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    • pp.474-499
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    • 2010
  • This paper presents the mostortant developments implemented in the APOLLO2 spectral code since its last general presentation at the 1999 M&C conference in Madrid. APOLLO2 has been provided with new capabilities in the domain of cross section self-shielding, including mixture effects and transfer matrix self-shielding, new or improved flux solvers (CPM for RZ geometry, heterogeneous cells for short MOC and the linear-surface scheme for long MOC), improved acceleration techniques ($DP_1$), that are also applied to thermal and external iterations, and a number of sophisticated modules and tools to help user calculations. The method of characteristics, which took over the collision probability method as the main flux solver of the code, allows for whole core two-dimensional heterogeneous calculations. A flux reconstruction technique leads to fast albeit accurate solutions used for industrial applications. The APOLLO2 code has been integrated (APOLLO2-A) within the $ARCADIA^{(R)}$ reactor code system of AREVA as cross section generator for PWR and BWR fuel assemblies. APOLLO2 is also extensively used by Electricite de France within its reactor calculation chain. A number of numerical examples are presented to illustrate APOLLO2 accuracy by comparison to Monte Carlo reference calculations. Results of the validation program are compared to the measured values on power plants and critical experiments.

Assessment of INSPYRE-extended fuel performance codes against the SUPERFACT-1 fast reactor irradiation experiment

  • L. Luzzi;T. Barani;B. Boer;A. Del Nevo;M. Lainet;S. Lemehov;A. Magni;V. Marelle;B. Michel;D. Pizzocri;A. Schubert;P. Van Uffelen;M. Bertolus
    • Nuclear Engineering and Technology
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    • v.55 no.3
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    • pp.884-894
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    • 2023
  • Design and safety assessment of fuel pins for application in innovative Generation IV fast reactors calls for a dedicated nuclear fuel modelling and for the extension of the fuel performance code capabilities to the envisaged materials and irradiation conditions. In the INSPYRE Project, comprehensive and physics-based models for the thermal-mechanical properties of U-Pu mixed-oxide (MOX) fuels and for fission gas behaviour were developed and implemented in the European fuel performance codes GERMINAL, MACROS and TRANSURANUS. As a follow-up to the assessment of the reference code versions ("pre-INSPYRE", NET 53 (2021) 3367-3378), this work presents the integral validation and benchmark of the code versions extended in INSPYRE ("post-INSPYRE") against two pins from the SUPERFACT-1 fast reactor irradiation experiment. The post-INSPYRE simulation results are compared to the available integral and local data from post-irradiation examinations, and benchmarked on the evolution during irradiation of quantities of engineering interest (e.g., fuel central temperature, fission gas release). The comparison with the pre-INSPYRE results is reported to evaluate the impact of the novel models on the predicted pin performance. The outcome represents a step forward towards the description of fuel behaviour in fast reactor irradiation conditions, and allows the identification of the main remaining gaps.

Assessment of three European fuel performance codes against the SUPERFACT-1 fast reactor irradiation experiment

  • Luzzi, L.;Barani, T.;Boer, B.;Cognini, L.;Nevo, A. Del;Lainet, M.;Lemehov, S.;Magni, A.;Marelle, V.;Michel, B.;Pizzocri, D.;Schubert, A.;Uffelen, P. Van;Bertolus, M.
    • Nuclear Engineering and Technology
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    • v.53 no.10
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    • pp.3367-3378
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    • 2021
  • The design phase and safety assessment of Generation IV liquid metal-cooled fast reactors calls for the improvement of fuel pin performance codes, in particular the enhancement of their predictive capabilities towards uranium-plutonium mixed oxide fuels and stainless-steel cladding under irradiation in fast reactor environments. To this end, the current capabilities of fuel performance codes must be critically assessed against experimental data from available irradiation experiments. This work is devoted to the assessment of three European fuel performance codes, namely GERMINAL, MACROS and TRANSURANUS, against the irradiation of two fuel pins selected from the SUPERFACT-1 experimental campaign. The pins are characterized by a low enrichment (~ 2 wt.%) of minor actinides (neptunium and americium) in the fuel, and by plutonium content and cladding material in line with design choices envisaged for liquid metal-cooled Generation IV reactor fuels. The predictions of the codes are compared to several experimental measurements, allowing the identification of the current code capabilities in predicting fuel restructuring, cladding deformation, redistribution of actinides and volatile fission products. The integral assessment against experimental data is complemented by a code-to-code benchmark focused on the evolution of quantities of engineering interest over time. The benchmark analysis points out the differences in the code predictions of fuel central temperature, fuel-cladding gap width, cladding outer radius, pin internal pressure and fission gas release and suggests potential modelling development paths towards an improved description of the fuel pin behaviour in fast reactor irradiation conditions.

Recent research progress on acid-growth theory (산-생장설에 대한 최근 연구 동향)

  • Lee, Sang Ho
    • Journal of Plant Biotechnology
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    • v.43 no.4
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    • pp.405-410
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    • 2016
  • Auxins are essential in plant growth and development. The auxin-stimulated elongation of plant cells has been explained by the "acid-growth theory", which was proposed forty years ago. According to this theory, the auxin activates plasma membrane $H^+-ATPase$ to induce proton extrusion into the apoplast, promoting cell expansion through the activation of cell wall-loosening proteins such as expansins. Even though accepted as the classical theory of auxin-induced cell growth for decades, the major signaling components comprising this model were unknown, until publication of recent reports. The major gap in the acid growth theory is the signaling mechanism by which auxin activates the plasma membrane $H^+-ATPase$. Recent genetic, molecular, and biochemical approaches reveal that several auxin-related molecules, such as TIR1/AFB AUX/IAA coreceptors and SMALL AUXIN UP RNA (SAUR), serve as important components of the acid-growth model, phosphorylating and subsequently activating the plasma membrane $H^+-ATPase$. These researches reestablish the four-decade-old theory by providing us the detailed signaling mechanism of auxininduced cell growth. In this review, we discuss the recent research progress in auxin-induced cell elongation, and a set of possible future works based on the reestablished acid-growth model.

SUBTOURNAMENTS ISOMORPHIC TO W5 OF AN INDECOMPOSABLE TOURNAMENT

  • Belkhechine, Houmem;Boudabbous, Imed;Hzami, Kaouthar
    • Journal of the Korean Mathematical Society
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    • v.49 no.6
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    • pp.1259-1271
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    • 2012
  • We consider a tournament T = (V,A). For each subset X of V is associated the subtournament T(X) = (X,$A{\cap}(X{\times}X)$) of T induced by X. We say that a tournament T' embeds into a tournament T when T' is isomorphic to a subtournament of T. Otherwise, we say that T omits T'. A subset X of V is a clan of T provided that for a, $b{\in}X$ and $x{\in}V{\backslash}X$, $(a,x){\in}A$ if and only if $(b,x){\in}A$. For example, ${\emptyset}$, $\{x\}(x{\in}V)$ and V are clans of T, called trivial clans. A tournament is indecomposable if all its clans are trivial. In 2003, B. J. Latka characterized the class ${\tau}$ of indecomposable tournaments omitting a certain tournament $W_5$ on 5 vertices. In the case of an indecomposable tournament T, we will study the set $W_5$(T) of vertices $x{\in}V$ for which there exists a subset X of V such that $x{\in}X$ and T(X) is isomorphic to $W_5$. We prove the following: for any indecomposable tournament T, if $T{\notin}{\tau}$, then ${\mid}W_5(T){\mid}{\geq}{\mid}V{\mid}$ -2 and ${\mid}W_5(T){\mid}{\geq}{\mid}V{\mid}$ -1 if ${\mid}V{\mid}$ is even. By giving examples, we also verify that this statement is optimal.