• Title/Summary/Keyword: ATP production

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In-vitro Production of Glutathione Using Yeast ATP Regeneration System and Recombinant Synthetic Enzymes from Escherichia coli. (효모의 ATP 재생산계와 대장균 유래의 재조합 생산효소를 이용한 in vitro 글루타치온 생산)

  • 고성영;구윤모
    • Microbiology and Biotechnology Letters
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    • v.26 no.3
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    • pp.213-220
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    • 1998
  • An ATP regeneration system was used for the production of glutathione which was synthesized by a sequential action of ${\gamma}$-glutamyl-cysteine synthetase and glutathione synthetase. The synthetases above were produced in the recombinant E. coli (TG1/pDG7) with the highest specific production yield of 31 mg glutathione/g wet cell. Bakers yeast was considered to have economically a better ATP regeneration system although the glutathione production yield was lower than that of acetate kinase. It was also observed that the ATP regeneration system of bakers yeast was superior to that of Saccharomyces cerevisiae ATCC24858. The yield of glutathione production with bakers yeast was 36% with the ATP concentration of 5 mM. To avoid the cysteine limitation during the early phase of glutatione production, an extra cysteine was added at 2 hours after reaction and the production yield increased 1.91 times. The effectiveness of bakers yeast as an ATP regeneration system was proved by several sets of extra feeding experiments. The product inhibition by glutathione above 14 mM was also observed.

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The Action of ATP on Phospholipase $A_2$Activation in C6 Cells (C6세포에서 phospholipase $A_2$활성에 대한 ATP의 작용)

  • 심상수;김명준;윤신희;김창종;조양혁
    • YAKHAK HOEJI
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    • v.45 no.4
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    • pp.413-418
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    • 2001
  • To investigate action of ATP on ischemia-induced brain injury, we measured phospholipase $A_2$activity and nitric oxide (NO) production in C6 cells. ATP alone did not have any influence on phospholipase $A_2$activity but increased NO production. Glutamate (1 mM) significantly increased phospholipase $A_2$activity whereas did not increased NO production. ATP significantly inhibited phospholipase $A_2$activation induced by 0.1 $\mu$M A23187, 1 mM glutamate and 1 mM $H_2O$$_2$, but did not inhibited 1 $\mu$M PMA-induced phospholipase $A_2$activation. From the above results, it is suggested that the action of ATP in C6 cells has dual actions, such as the inhibition of agonist-induced phospholipase $A_2$activation and the increase of NO production.

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Deletion of cg1360 Affects ATP Synthase Function and Enhances Production of L-Valine in Corynebacterium glutamicum

  • Wang, Xiaochen;Yang, Hongyu;Zhou, Wei;Liu, Jun;Xu, Ning
    • Journal of Microbiology and Biotechnology
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    • v.29 no.8
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    • pp.1288-1298
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    • 2019
  • Bacterial ATP synthases drive ATP synthesis by a rotary mechanism, and play a vital role in physiology and cell metabolism. Corynebacterium glutamicum is well known as an industrial workhorse for amino acid production, and its ATP synthase operon contains eight structural genes and two adjacent genes, cg1360 and cg1361. So far, the physiological functions of Cg1360 (GenBank CAF19908) and Cg1361 (GenBank CAF19909) remain unclear. Here, we showed that Cg1360 was a hydrophobic protein with four transmembrane helices (TMHs), while no TMH was found in Cg1361. Deletion of cg1360, but not cg1361, led to significantly reduced cell growth using glucose and acetic acid as carbon sources, reduced F1 portions in the membrane, reduced ATP-driven proton-pumping activity and ATPase activity, suggesting that Cg1360 plays an important role in ATP synthase function. The intracellular ATP concentration in the ${\Delta}cg1360$ mutant was decreased to 72% of the wild type, while the NADH and NADPH levels in the ${\Delta}cg1360$ mutant were increased by 29% and 26%, respectively. However, the ${\Delta}cg1361$ mutant exhibited comparable intracellular ATP, NADH and NADPH levels with the wild-type strain. Moreover, the effect of cg1360 deletion on L-valine production was examined in the L-valine-producing V-10 strain. The final production of L-valine in the $V-10-{\Delta}cg1360$ mutant reached $9.2{\pm}0.3g/l$ in shake flasks, which was 14% higher than that of the V-10 strain. Thus, Cg1360 can be used as an effective engineering target by altering energy metabolism for the enhancement of amino acid production in C. glutamicum.

Order Promising Rolling Planning with ATP/CTP Reallocation Mechanism

  • Chen, Juin-Han;Lin, James T.;Wu, Yi-Sheng
    • Industrial Engineering and Management Systems
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    • v.7 no.1
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    • pp.57-65
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    • 2008
  • Available-to-promise (ATP) exhibiting availability of manufacturing resources can be used to support customer order promising. Recently, one advanced function called Capable-to-promise (CTP) is provided by several modern APS (advanced planning system) that checks available capacity for placing new production orders or increasing already scheduled production orders. At the customer enquiry stage while considering the order delivery date and quantity to quote, both ATP and CTP are allocated to support order promising. In particular, current trends of mass customization and multi-side production chain derive several new constraints that should be considered when ATP/CTP allocation planning for order promising - such as customer's preference plants or material vendors, material compatibility, etc. Moreover, ATP/CTP allocation planning would be executed over a rolling time horizon. To utilize capacity and material manufacturing resource flexibly and fulfill more customer orders, ATP/CTP rolling planning should possess resource reallocation mechanism under the constraints of order quantities and delivery dates for all previous order promising. Therefore, to enhance order promising with reliability and flexibility to reallocate manufacturing resource, the ATP/CTP reallocation planning mechanism is needed in order to reallocate material and capacity resource for fulfilling all previous promised and new customer orders beneficially with considering new derived material and capacity constraints.

Development of Intelligent ATP System Using Genetic Algorithm (유전 알고리듬을 적용한 지능형 ATP 시스템 개발)

  • Kim, Tai-Young
    • Journal of Intelligence and Information Systems
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    • v.16 no.4
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    • pp.131-145
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    • 2010
  • The framework for making a coordinated decision for large-scale facilities has become an important issue in supply chain(SC) management research. The competitive business environment requires companies to continuously search for the ways to achieve high efficiency and lower operational costs. In the areas of production/distribution planning, many researchers and practitioners have developedand evaluated the deterministic models to coordinate important and interrelated logistic decisions such as capacity management, inventory allocation, and vehicle routing. They initially have investigated the various process of SC separately and later become more interested in such problems encompassing the whole SC system. The accurate quotation of ATP(Available-To-Promise) plays a very important role in enhancing customer satisfaction and fill rate maximization. The complexity for intelligent manufacturing system, which includes all the linkages among procurement, production, and distribution, makes the accurate quotation of ATP be a quite difficult job. In addition to, many researchers assumed ATP model with integer time. However, in industry practices, integer times are very rare and the model developed using integer times is therefore approximating the real system. Various alternative models for an ATP system with time lags have been developed and evaluated. In most cases, these models have assumed that the time lags are integer multiples of a unit time grid. However, integer time lags are very rare in practices, and therefore models developed using integer time lags only approximate real systems. The differences occurring by this approximation frequently result in significant accuracy degradations. To introduce the ATP model with time lags, we first introduce the dynamic production function. Hackman and Leachman's dynamic production function in initiated research directly related to the topic of this paper. They propose a modeling framework for a system with non-integer time lags and show how to apply the framework to a variety of systems including continues time series, manufacturing resource planning and critical path method. Their formulation requires no additional variables or constraints and is capable of representing real world systems more accurately. Previously, to cope with non-integer time lags, they usually model a concerned system either by rounding lags to the nearest integers or by subdividing the time grid to make the lags become integer multiples of the grid. But each approach has a critical weakness: the first approach underestimates, potentially leading to infeasibilities or overestimates lead times, potentially resulting in excessive work-inprocesses. The second approach drastically inflates the problem size. We consider an optimized ATP system with non-integer time lag in supply chain management. We focus on a worldwide headquarter, distribution centers, and manufacturing facilities are globally networked. We develop a mixed integer programming(MIP) model for ATP process, which has the definition of required data flow. The illustrative ATP module shows the proposed system is largely affected inSCM. The system we are concerned is composed of a multiple production facility with multiple products, multiple distribution centers and multiple customers. For the system, we consider an ATP scheduling and capacity allocationproblem. In this study, we proposed the model for the ATP system in SCM using the dynamic production function considering the non-integer time lags. The model is developed under the framework suitable for the non-integer lags and, therefore, is more accurate than the models we usually encounter. We developed intelligent ATP System for this model using genetic algorithm. We focus on a capacitated production planning and capacity allocation problem, develop a mixed integer programming model, and propose an efficient heuristic procedure using an evolutionary system to solve it efficiently. This method makes it possible for the population to reach the approximate solution easily. Moreover, we designed and utilized a representation scheme that allows the proposed models to represent real variables. The proposed regeneration procedures, which evaluate each infeasible chromosome, makes the solutions converge to the optimum quickly.

Impact of glucose and pyruvate on adenosine triphosphate production and sperm motility in goats

  • Rangga Setiawan;Raden Febrianto Christi;Ken Ratu Gharizah Alhuur;Rini Widyastuti;Nurcholidah Solihati;Siti Darodjah Rasad;Kundrat Hidajat;Duy Ngoc Do
    • Animal Bioscience
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    • v.37 no.4
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    • pp.631-639
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    • 2024
  • Objective: This study evaluates goat sperm motility in response to metabolic substrates and various inhibitors, aiming to assess the relative contribution of glycolysis and mitochondrial oxidation for sperm movement and adenosine triphosphate (ATP) production. Methods: In the present study, two main metabolic substrates; 0 to 0.5 mM glucose and 0 to 30 mM pyruvate were used to evaluate their contribution to sperm movements of goats. Using a 3-chloro-1,2-propanediol (3-MCPD), a specific inhibitor for glycolysis, and carbonyl cyanide 3-chlorophenylhydrazone as an inhibitor for oxidative phosphorylation, cellular mechanisms into ATP-generating pathways in relation to sperm movements and ATP production were observed. Data were analysed using one-way analysis of variance for multiple comparisons. Results: Sperm motility analysis showed that either glucose or pyruvate supported sperm movement during 0 to 30 min incubation. However, the supporting effects were abolished by the addition of a glycolysis inhibitor or mitochondrial uncoupler, concomitant with a significant decrease in ATP production. Although oxidative phosphorylation produces larger ATP concentrations than those from glycolysis, sperm progressivity in relation to these two metabolic pathways is comparable. Conclusion: Based on the present study, we suggest that goat sperm use glucose and pyruvate to generate cellular energy through glycolysis and mitochondrial respiration pathways to maintain sperm movement.

An ATP System with Non-Integer Time Lag for Supply Chain Management (공급 사슬 관리를 위한 비정수 타임 랙을 갖는 ATP 시스템)

  • Kim, Tai-Young
    • 한국경영정보학회:학술대회논문집
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    • 2008.06a
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    • pp.478-485
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    • 2008
  • 고객 만족과 납기 충족율을 최대화하기 위하여, 정확하고 실용적인 납기회답 시스템(ATP)은 매우 중요한 역할을 한다. 그러나 복잡한 공급사슬관리 환경 하에서 조달, 제조, 분배 등을 모두 고려한 정확한 ATP 수량 할당은 매우 어려운 업무이고, 때문에 많은 연구들이 이루어져 왔다. 지금까지 기존의 선행 연구에서 시도되었던 ATP 모형들은 공통적으로 정수배의 시간 단위만 고려해 왔고, 이는 실제 산업 현장의 ATP 프로세스를 정확하게 반영하지 못하고 있는 비현실적인 가정이라 할 수 있다. 본 논문에서는 SCM을 위하여 비정수 타임 랙을 사용한 ATP 시스템을 고려한다. 기존 연구들에서 이산형의 무리한 가정으로 표현되어 왔던 시간 단위를 동적 생산 함수(dynamic production function) 개념을 통하여 비음의 실수 범위에서의 자유롭게 나누어 고려할 수 있도록 하였다. 이를 통하여 기존 ATP 연구들의 무리한 가정을 제거하였으며, 보다 더 현실에 가까운 ATP 모델을 제안한다. 본 논문에서는 특히 공급 사슬(Supply Chain) 전체의 재고와 생산, 운송을 모두 고려하며 고객 주문에 대응하는 통합 ATP 시스템을 설계하였고, 기존 연구들이 미처 고려하지 못한 시간 흐름의 연속성에 중점을 두고 선형 계획(LP) 문제의 형태로 비정수 타임랙(non-integer time lag)을 갖는 ATP 시스템을 모델링하였다.

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The uniqueness of the plant mitochondrial potassium channel

  • Pastore, Donato;Soccio, Mario;Laus, Maura Nicoletta;Trono, Daniela
    • BMB Reports
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    • v.46 no.8
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    • pp.391-397
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    • 2013
  • The ATP-inhibited Plant Mitochondrial $K^+$ Channel ($PmitoK_{ATP}$) was discovered about fifteen years ago in Durum Wheat Mitochondria (DWM). $PmitoK_{ATP}$ catalyses the electrophoretic $K^+$ uniport through the inner mitochondrial membrane; moreover, the co-operation between $PmitoK_{ATP}$ and $K^+/H^+$ antiporter allows such a great operation of a $K^+$ cycle to collapse mitochondrial membrane potential (${\Delta}{\Psi}$) and ${\Delta}pH$, thus impairing protonmotive force (${\Delta}p$). A possible physiological role of such ${\Delta}{\Psi}$ control is the restriction of harmful reactive oxygen species (ROS) production under environmental/oxidative stress conditions. Interestingly, DWM lacking ${\Delta}p$ were found to be nevertheless fully coupled and able to regularly accomplish ATP synthesis; this unexpected behaviour makes necessary to recast in some way the classical chemiosmotic model. In the whole, $PmitoK_{ATP}$ may oppose to large scale ROS production by lowering ${\Delta}{\Psi}$ under environmental/oxidative stress, but, when stress is moderate, this occurs without impairing ATP synthesis in a crucial moment for cell and mitochondrial bioenergetics.

Bacillus anthracis Spores Influence ATP Synthase Activity in Murine Macrophages

  • Seo, Gwi-Moon;Jung, Kyoung-Hwa;Kim, Seong-Joo;Kim, Ji-Cheon;Yoon, Jang-Won;Oh, Kwang-Keun;Lee, Jung-Ho;Chai, Young-Gyu
    • Journal of Microbiology and Biotechnology
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    • v.18 no.4
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    • pp.778-783
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    • 2008
  • Anthrax is an infectious disease caused by toxigenic strains of the Gram-positive bacterium Bacillus anthracis. To identify the mitochondrial proteins that are expressed differently in murine macrophages infected with spores of B. anthracis Sterne, proteomic and MALDI-TOF/MS analyses of uninfected and infected macrophages were conducted. As a result, 13 mitochondrial proteins with different expression patterns were discovered in the infected murine macrophages, and some were identified as ATP5b, NIAP-5, ras-related GTP binding protein B isoform CRAa, along with several unnamed proteins. Among these proteins, ATP5b is related to energy production and cytoskeletal rearrangement, whereas NIAP-5 causes apoptosis of host cells due to binding with caspase-9. Therefore, this paper focused on ATP5b, which was found to be down regulated following infection. The downregulated ATP5b also reduced ATP production in the murine macrophages infected with B. anthracis spores. Consequently, this study represents the first mitochondrial proteome analysis of infected macrophages.

Factors Afecting Hydrogen Evolution in Chlorobium limicola f. theosulfatophilum NCIB 8327 (Chlorobium limicola f. thiosulfatophilum NCIB 8327에서 수소발생에 영향을 끼치는 요인분석)

  • 나종욱;강사욱
    • Korean Journal of Microbiology
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    • v.30 no.6
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    • pp.553-557
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    • 1992
  • Hydrogen produced by cells of grown Chlorobium limicola f. thiosulfatophilum NCIB 8327 on modified Pfennig's medium containing glutamate as a major nitrogen source, was measured by amperometric method. In this system, oxygen, light. ammonia, methionine sulfoximine, NADPH, ATP, methyl viologen and benzyl viologen are affected. The production of hydrogen in intact cells depends on light intensity. It is also inhibited by adding ammonium ions, but restores immediately by adding methionine sulfoximine. Considering these results, the production of hydrogen in this strain can be mediated by nitrogenase.

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