• Applied Biological Chemistry
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• v.40 no.6
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• pp.465-471
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• 1997

The cyclodextrin glycosyltransferase (CGTase, EC 3.2.1.19) from Bacillus brevis CD162 was purified by precipitating with ammonium sulfate, DEAE-Sepharose CL-6B column chromatography and Sephadex G-150 column chromatography. The molecular mass and pI of the purified enzyme were estimated to be 74,000 and 6.3 by SDS-PAGE and isoelectric focusing, respectively. The purified enzyme was clearly identified as the CGTase by zymogram after SDS-PAGE. The optimum pH and temperature for the enzyme activity were 8.0 and $55^{\circ}C$, respectively. The enzyme was stable at the range of pH $5.5{\sim}9.0$, and up to $50^{\circ}C$. The amino acid sequence from the $NH_2-terminal$ of the purified CGTase was Ser-Val-Thr-Asn-Lys-Val-Asn-Tyr-Ser-Lys-Asp-Val-Ile-Tyr-Gln. The yields of the products from starch as the substrate were 1.3% for ${\alpha}-$, 33.9% for ${\beta}-$, and 9.7% for ${\gamma}-cyclodextrin$.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.16
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• pp.6619-6625
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• 2014

Background: Numerous studies have explored the influence of XPD Lys751Gln and/or Asp312Asn polymorphisms on skin cancer susceptibility. However, the results remain inconclusive. To derive a more precise estimation, we conducted a comprehensive search to identify all available published studies and performed a meta-analysis. Materials and Methods: Electronic literature searches of the PubMed, CBM and CNKI databases were performed up to March 2014. Odds ratios (ORs) with 95% confidence intervals (CIs) were applied to assess the strength of associations. Results: Seventeen case-control studies were included with a total sample size of 6, 113 cases and 11, 074 controls for the XPD Lys751Gln polymorphism, and 10 studies (3, 840cases and 7, 637 controls) for the XPD Asp312Asn polymorphism were pooled for analysis. Overall, no significant associations were found between the XPD Lys751Gln polymorphism and skin cancer risk in any genetic model. On stratified analysis by tumor type, XPD Lys751Gln polymorphism was not associated with increased risk of non-melanoma skin cancer, but was significantly related with increased risk of cutaneous melanoma (Gln/Gln vs Lys/Lys: OR=1.15, 95%CI=1.02-1.29, p=0.023; dominant model: OR=1.09, 95%CI=1.01-1.18, p=0.036). For the XPD Asp312Asn polymorphism, no significant association with skin cancer risk was observed in overall or subgroup analyses. Conclusions: The present meta-analysis suggests that the XPD Lys751Gln polymorphism may contribute to the risk of cutaneous melanoma from currently available evidence. Further investigations are needed to obtain more insight into possible roles of these two polymorphisms in skin carcinogenesis.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.1
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• pp.449-452
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• 2013

Polymorphisms in DNA repair genes have been shown to influence DNA repair processes and to modify cancer susceptibility. Here we conducted a case-control study to assess the role of potential SNPs of DNA repair genes on the risk of glioma and meningioma. We included 297 cases and 458 cancer-free controls. Genotyping of XRCC1 Gln399Arg, XRCC1 Arg194Trp, XRCC2 Arg188His, XRCC3 Thr241Met, XRCC4 Ala247Ser, ERCC1 Asn118Asp, ERCC2 Lys751Gln and ERCC5 Asp1558His were performed in a 384-well plate format on the Sequenom MassARRAY platform. XRCC1 Arg194Trp (rs1799782) and ERCC2 Asp312Asn rs1799793 did not follow the HWE in control group, and genotype distributions of XRCC1 Gln399Arg rs25487, XRCC2 Arg188His rs3218536 and ERCC2 Asp312Asn rs1799793 were significantly different between cases and controls (P<0.05). We found XRCC1 399G/G, XRCC1 194 T/T and XRCC3 241T/T were associated with a higher risk when compared with the wild-type genotype. For ERCC5 Asp1558His, we found G/G genotype was associated with elevated susceptibility. In conclusion, our study has shown that XRCC1 Gln399Arg, XRCC1 Arg194Trp, XRCC3 Thr241Met and ERCC5 Asp1558His are associated with risk of gliomas and meningiomas. This finding could be useful in identifying the susceptibility genes for these cancers.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.8 no.5
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• pp.71-84
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• 2009

The communication between the center system and the local controller in the Traffic Signal Controller Specification has been specified by a communication line for the exclusive use and the analog modem serial protocol. Therefore, it can't be adapted in the ITS communication network of the TCP/IP protocol being constructed in the local city. The international and domestic ITS device's communication specification has adapted the DATEX-ASN data exchange technique based on the ethernet communication network. So, this study was performed by the purpose of developing the application communication protocol's standard draft based on the TCP/IP communication protocol for the traffic signal controller, to be able to constitute the traffic signal control system in the ITS communication network. The communication format for 23 items of control, status, and database request etc. which are specified in the standard traffic signal controller was developed by appling the DATEX-ASN data interface procedure and structure which are KS ISO X 14827 Part1, 2, ISO/ DIS 15784 Part-3. To test the developed protocol, the application program for the communication items was developed and according to the test result, the encoding and the decoding transection for all communication items was possible.

• Fisheries and Aquatic Sciences
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• v.5 no.1
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• pp.54-58
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• 2002

The polymerase chain reaction (PCR) amplification technique was used for comparison of Listeria monocytogenes serotypes. PCR primers for the fragment of invasion-associated protein (iap) gene were highly specific for all the serotypes of L. monocytogenes. Other Listeria spp., such as Listeria ivanovii and Listeria innocua were not produced the PCR fragments by above primer set. The nucleotide sequences of PCR products showed high homologies in comparison of all the isolated serotypes except unknown type II-2. The deduced amino acid sequences of the PCR products also showed similar to one another. The various region of the PCR products, called a Thr-Asn repeat region was presented. All of isolated L. monocytogenes serotypes possessed 16 to 20 Thr-Asn repeats.

• JOURNAL OF ELECTRICAL WORLD
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• s.278
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• pp.64-69
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• 2000

미국과 유럽에서 추진되고 있는 ITS(Intelligent Transport Systems : 고도도로교통시스템) 도로통신에서 취급하는 정보의 정의, 통신방식의 표준화 등의 동향에 대한 개요를 기술한다. 미국에서는 NTCIP(National Transportation Communication for ITS Protocol)라 불리우는 통신프로토콜, 데이터 딕셔너리, 메시지 셋트 등의 표준화가, 유럽에서는 DATEX-Net라는 데이터 교환의 표준화가 추진되고 있다. 이들은 ITS 분야에서의 시스템 간 및 장치간의 상호운용성$\cdot$호환성을 확보하고 통합적으로 ITS를 전개해가기 위한 기반정비의 주요 항목이다. 양자 공히 표준적인 통신프로토콜이 채용되고 있으나 정보내용, 메시지포맷에 대하여는 NTCIP가 ASN.1(Abstract Systax Notation 1)에서 기술한 MIB(Management Information Base)구조를 갖고 있는 한편, Datex-NET는 독자적인 구조의 사전과 EDIFACT(Electronic Data Interchange for Administration Commerce and Transport)에 따른 메시지사양으로 되어 있어 공통성이 없다. 그러나 센터간 통신에 관하여는 미국에서 제안한 DATEX-ASN이라는 표준화 안에 대하여 유럽에서 전향적인 평가를 하고 있어 통일화의 징조가 보이고 있다.

• Proceedings of the Korea Information Processing Society Conference
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• 2006.05a
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• pp.333-336
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• 2006

HERMES(tHE Retrieval framework for visual MEdia Service)는 웹 상에 존재하는 그림, 영상 등 시각미디어 검색을 위한 시스템이다. 복수의 서비스 제공자와 하나의 서비스 중계자가 있는 분산 환경에서, 사용자의 질의가 왔을 때 서비스 중계자는 적절한 서비스 제공자를 효율적으로 찾는 것이 매우 중요하다. 그래서 다양한 서비스 제공자가 존재하는 분산환경에서 서비스 제공자들간의 랭킹은 매우 중요하다. 본 논문에서는 온톨로지를 사용하여 서비스 제공자들을 랭킹하는 방법을 제안하고 기존의 서비스 제공자 랭킹과 비교하여 본 논문에서 제안한 방법이 더욱 우수함을 보인다.

• BMB Reports
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• v.31 no.1
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• pp.37-43
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• 1998

The catabolic ${\alpha}$-acetolactate synthase was purified to homogeneity from Serratia marcescens ATCC 25419 using ammonium sulfate fractionation, DEAE-Sepharose, Phenyl-Sepharose, and Hydroxylapatite column chromatography. The native molecular weight of the enzyme was approximately 150 kDa and composed of two identical subunits with molecular weights of 64 kDa each. The N-terminal amino acid sequence of the enzyme was determined to be Ala-Gln-Glu-Lys-Thr-Gly-Asn-Asp-Trp-Gln-His-Gly-Ala-Asp-Leu-Val-Val-Lys-Asn-Leu. It was not inhibited by the branched chain amino acids and sulfometuron methyl herbicide. The optimum pH of the enzyme was around pH 5.5 and the pI value was 6.1. The catabolic ${\alpha}$-acetolactate synthase showed weak immunological relationships with recombinant tobacco ALS, barley ALS, and the valine-sensitive ALS isozyme from Serratia marcescens.

• Journal of Korean Society for Quality Management
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• v.29 no.1
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• pp.113-127
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• 2001

Skip-lot sampling plans for lot-by-lot inspection are more desirable than the continuous sampling plans for units in modern mass production system under the condition of the submitted product is good. Perry(1973a, 1973b) extended the Dodge(1955)′s skip-lot sampling plans to single and two-level skip-lot sampling plans and Parker & Kessler (1981) modified Perry(1973a)′s plans so that a sample of size one is taken from every lot skipped during the skipping mode. In this paper, Perry(1973b)′s two-level skip-lot sampling plans are modified by applying Parker & Kessler′s plan, designated as MTSkSP1, MTSkSP2, MTSkSP3. Operating characteristic(OC) functions, average sample numbers(ASN) and average outgoing qualities(AOQ) for the proposed plans are derived using Markov chain properties and compared each other and Perry′s plans. The proposed plans not only reduce the ASN but also avoid the danger skipping lots entirely when the lots are produced during sudden "out-of-control".

• 지반과기술
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• v.9 no.2
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• pp.5-17
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• 2012