• 제목/요약/키워드: AS/RS

검색결과 1,500건 처리시간 0.03초

Differential Influences in Sizes and Cell Cycle Stages of Donor Blastomeres on the Development of Cloned Rabbit Embryos

  • Ju, Jyh-Cherng;Yang, Jyh-Shyu;Liu, Chien-Tsung;Chen, Chien-Hong;Tseng, Jung-Kai;Chou, Po-Chien;Cheng, San-Pao
    • Asian-Australasian Journal of Animal Sciences
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    • 제16권1호
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    • pp.15-22
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    • 2003
  • Experiments were conducted to evaluate the effect of blastomere diameters and cell cycle stages on the subsequent development of nuclear transplant rabbit embryos (NT-embryos) using nuclei derived from the 16- or 32-cell stage embryos. All blastomeres and NT-embryos were cultured individually in modified Ham's F-10 medium supplemented with 10% rabbit serum (RS) at $38^{\circ}C$ and 5% $CO_2$ in air. The diameter of blastomeres from 16-cell stage embryos was found twice of those from 32-cell stage (51 vs 27 ${\mu}m$). Significant differences were observed in cleavage rates ($\geq$3 divisions) in the isolated single blastomeres (54 vs 48 for 16-cell; 28 vs 14 for 32-cell, p<0.05), but the fusion rates of oocytes with transferred nuclei were similar between small and large single blastomeres derived from either 16-cell or 32-cell stage embryos. When 16-cell stage blastomeres were used as nuclear donors, cleavage rates ($\geq$3 divisions) of the NT-embryos were greater in the small nuclear donors than in the large donors (73 vs 55%, p<0.05). On the contrary, significantly higher cleavage (43 vs 6%, p<0.05) and developmental rates (14 vs 0%, p<0.05) were observed in the large blastomere nuclear donor group of the 32-cell stage embryos. When the cell cycle stages were controlled by a microtubule polymerization inhibitor (Demicolcine, DEM) or the combined treatment of DEM and Aphidicolin (APH), a DNA polymerase inhibitor, fusion rates were 88-96% for the 16-cell donor group (without DEM treatment), which were greater than the 32-cell donor group (54-58%). Cleavage rates were also greater in the transplants derived from G1 nuclear donor group (93-95%) than those from the DEM and APH combined treatment (73%) for the 16-cell donor group (p<0.05). No significant difference was detected in the morula/blastocyst rates in either donor cell stage (p>0.05). In conclusion, it appeared that no difference in the developmental competence between large and small isolated blastomeres was observed. When smaller 16-cell stage blastomeres were used as nuclear donor, the cleavage rate or development of NT-embryos was improved and was compromised when 32-cell stage blastomeres were used. Therefore, control nuclear stage of the donor cell at $G_1$ phase in preactivated nuclear recipients seemed to be beneficial for the cleavage rate of the reconstructed embryo in the 16-cell transplant, but not for subsequent morula or blastocyst development.

Characteristics of Underwater Acoustic Channel and Performance of Multi-Carrier System in Littoral Ocean near Busan City (부산인근 해역의 수중음향통신 채널특성과 다중반송파 시스템의 성능)

  • Kim, Jongjoo;Park, Jihyun;Bae, Minja;Yoon, Jong Rak
    • Journal of the Korea Institute of Information and Communication Engineering
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    • 제21권12호
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    • pp.2394-2402
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    • 2017
  • The frequency selective fading by multipaths determines a performance of underwater acoustic communication system in shallow littoral ocean. In this study, a characteristics of underwater acoustic channel and performance of multi-carrier system is evaluated in littoral ocean with a 50m deep water, an effective wave height of 0.5m and sandy mud bottom near Busan city. A multipath delay spread and time and frequency domain are presented as a function of a transmitter-to-receiver range. A bit-error-rate of a 5 channel 4FSK(Frequency Shift Keying) with a transmission rate of 1kbps, is examined and RS(Reed-Solomon) code is also adopted to remove a burst error due to time domain fading. A number of multipath are less than four and a bit-error-rate is decreased as an increase of a transmitter-to-receiver range which gives a congestion of multi-paths resulting in a decrease of time and frequency domain fading. The measured bit-error-rate is about 10-4 at greater than 600m of transmitter-to-receiver range.

A base study of an Ecological Mapping technique by using GIS and Remote Sensing (GIS와 RS를 이용한 생태지도 작성기법에 관한 기초연구)

  • Yi, Gi-Chul;Lee, Won-Hwa;Yoon, Hae-Soon;Nam, Chun-Hee;Kim, Gu-Yeon;Kim, Seong-Hwan;Suh, Sang-Hyun
    • Journal of the Korean Association of Geographic Information Studies
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    • 제7권3호
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    • pp.57-69
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    • 2004
  • This study developed an ecological mapping technique with GIS database using the analyses of existing ecological survey reports and the change detection on the Nakdong river estuary. The data which are used to establish GIS DB include 2 Landsat TM images on Nov. 31, 1984 and May 17, 1997, 1:25,000 topographical maps established by National Geography Institution and various ecological survey reports published by Busan metropolitan city government. The details for producing ecological map are as follows. At first, the current methods of ecomapping efforts and previous ecological surveys of Nakdong river estuary were carefully examined. Secondly, the land cover maps were created from the classified Landsat images of 1984 and 1997 for the spatiotemporal ecosystem analysis. Thirdly, the ecosystem was evaluated by using GIS ecological database based on the criteria of botany, zoology and water quality etc. Each criteria was reclassified into 3 stages which describe the overall quality of ecological condition. At last, the comprehensive ecological map was suggested as a prototype of ecosystem assesment and management tool with the discussion of further study. The findings of this study would be a milestone for preserving and managing the ecosystem.

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Analysis of Hydrological Surface Characteristics using ArcView/Spatial Analyst GIS Extension (ArcView/spatial Analyst GIS 확장 프로그램을 이용한 수리지형 특성인자 분석)

  • Lee, Ki-Won
    • Journal of the Korean earth science society
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    • 제22권6호
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    • pp.491-499
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    • 2001
  • Generally, several hydrological characteristics which can be obtained from geographic processing in GIS S/W would be utilized to actual hydrological applications such as landslide stability or drainage analysis. Furthermore, by using practical GIS extension programs, it can be possible that automatic extraction task of those information, which was time-consuming and complex processing, is performed as systematical and automatic processing. In this study, using ArcView GIS S/W and its extensions concerning hydrological processing, some waterflow and surface factors in the area of Yemi sheet scaled 1:50,000 were extracted. Then, assessment of landslide stability, determined by DEM-based variables were carried out in order to investigate practical applicability of extension programs. It is thought that several hydrological factors extracted by this study were significant information to predict field model or hydrological hazardous events. It also reveals that landslide potential is overall stable with stability index mapping and S-A plot, with theoretical basis. This methodology can be applicable to other areas related to hydrological characteristics with measurements in fields.

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Automated Algorithm for Super Resolution(SR) using Satellite Images (위성영상을 이용한 Super Resolution(SR)을 위한 자동화 알고리즘)

  • Lee, S-Ra-El;Ko, Kyung-Sik;Park, Jong-Won
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • 제18권2호
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    • pp.209-216
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    • 2018
  • High-resolution satellite imagery is used in diverse fields such as meteorological observation, topography observation, remote sensing (RS), military facility monitoring and protection of cultural heritage. In satellite imagery, low-resolution imagery can take place depending on the conditions of hardware (e.g., optical system, satellite operation altitude, image sensor, etc.) even though the images were obtained from the same satellite imaging system. Once a satellite is launched, the adjustment of the imaging system cannot be done to improve the resolution of the degraded images. Therefore, there should be a way to improve resolution, using the satellite imagery. In this study, a super resolution (SR) algorithm was adopted to improve resolution, using such low-resolution satellite imagery. The SR algorithm is an algorithm which enhances image resolution by matching multiple low-resolution images. In satellite imagery, however, it is difficult to get several images on the same region. To take care of this problem, this study performed the SR algorithm by calibrating geometric changes on images after applying automatic extraction of feature points and projection transform. As a result, a clear edge was found just like the SR results in which feature points were manually obtained.

Effect of frequency dependent multipath fading on non-coherent underwater communication system (주파수 종속 다중경로 페이딩이 비코히어런트 수중통신시스템에 미치는 영향)

  • Kim, Jongjoo;Park, Jihyun;Bae, Minja;Park, Kyu-Chil;Yoon, Jong Rak
    • The Journal of the Acoustical Society of Korea
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    • 제35권4호
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    • pp.295-302
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    • 2016
  • Underwater acoustic communication channel is often defined as a multipath fading channel since the multipath arrivals from various paths interfere with each other and cause frequency dependent constructive or destructive interference in received signals. Therefore signal-to-noise ratio (SNR) of received signal fluctuates as a function of frequency. In addition, sea surface fluctuation induces frequency dependent time variant signal fading due to coherent component variation of surface bounce path. The frequency shift keying (FSK) system is known to be less sensitive and more robust under these interference and fading, and M-ary frequency shift keying (MFSK) system is adopted to increase a data rate. In this study, a bit error rate (BER) of 4 channels 4FSK system are examined in shallow sea multipath channel. Experimental results show that RS code reduces efficiently the BER of 4FSK system since frequency dependent time-varying fading is characterized to give burst errors. The BER of a different data rate or different source-to-receiver range depends on not only the channel coherent bandwidth but also frequency dependent multipath fading.

Process Networks of Ecohydrological Systems in a Temperate Deciduous Forest: A Complex Systems Perspective (온대활엽수림 생태수문계의 과정망: 복잡계 관점)

  • Yun, Juyeol;Kim, Sehee;Kang, Minseok;Cho, Chun-Ho;Chun, Jung-Hwa;Kim, Joon
    • Korean Journal of Agricultural and Forest Meteorology
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    • 제16권3호
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    • pp.157-168
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    • 2014
  • From a complex systems perspective, ecohydrological systems in forests may be characterized with (1) large networks of components which give rise to complex collective behaviors, (2) sophisticated information processing, and (3) adaptation through self-organization and learning processes. In order to demonstrate such characteristics, we applied the recently proposed 'process networks' approach to a temperate deciduous forest in Gwangneung National Arboretum in Korea. The process network analysis clearly delineated the forest ecohydrological systems as the hierarchical networks of information flows and feedback loops with various time scales among different variables. Several subsystems were identified such as synoptic subsystem (SS), atmospheric boundary layer subsystem (ABLS), biophysical subsystem (BPS), and biophysicochemical subsystem (BPCS). These subsystems were assembled/disassembled through the couplings/decouplings of feedback loops to form/deform newly aggregated subsystems (e.g., regional subsystem) - an evidence for self-organizing processes of a complex system. Our results imply that, despite natural and human disturbances, ecosystems grow and develop through self-organization while maintaining dynamic equilibrium, thereby continuously adapting to environmental changes. Ecosystem integrity is preserved when the system's self-organizing processes are preserved, something that happens naturally if we maintain the context for self-organization. From this perspective, the process networks approach makes sense.

Development of a Prototype Patient Monitoring System with Module-Based Bedside Units and Central Stations: Overall Architecture and Specifications (모듈형 환자감시기와 중앙 환자감시기로 구성되는 환자감시시스템 시제품의 개발: 전체구조 및 사양)

  • Woo, E.J.;Park, S.H.;Jun, B.M.;Moon, C.W.;Lee, H.C.;Kim, S.T.;Kim, H.J.;Seo, J.J.;Chae, K.M.;Park, J.C.;Choi, K.H.;Lee, W.J.;Kim, K.S.
    • Proceedings of the KOSOMBE Conference
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    • 대한의용생체공학회 1996년도 춘계학술대회
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    • pp.315-319
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    • 1996
  • We have developed a prototype patient monitoring system including module-based bedside units, interbed network, and central stations. A bedside unit consists of a color monitor and a main CPU unit with peripherals including a module controller. It can also include up to 3 module cases and 21 different modules. In addition to the 3-channel recorder module, six different physiological parameters of ECG, respiration, invasive blood pressure, noninvasive blood pressure, body temperature, and arterial pulse oximetry with plethysmogaph are provided as parameter modules. Modules and a module controller communicate with up to 1Mbps data rate through an intrabed network based on RS-485 and HDLC protocol. Bedside units can display up to 12 channels of waveforms with any related numeric informations simultaneously. At the same time, it communicates with other bedside units and central stations through interbed network based on 10Mbps Ethernet and TCP/IP protocol. Software far bedside units and central stations fully utilizes gaphical user interface techniques and all functions are controlled by a rotate/push button on bedside unit and a mouse on central station. The entire system satisfies the requirements of AAMI and ANSI standards in terms of electrical safety and performances. In order to accommodate more advanced data management capabilities such as 24-hour full disclosure, we are developing a relational database server dedicated to the patient monitoring system. We are also developing a clinical workstation with which physicians can review and examine the data from patients through various kinds of computer networks far diagnosis and report generation. Portable bedside units with LCD display and wired or wireless data communication capability will be developed in the near future. New parameter modules including cardiac output, capnograph, and other gas analysis functions will be added.

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Validation of an analytical method of dieckol for standardization of Ecklonia cava extract as a functional ingredient (감태추출물의 기능성원료 표준화를 위한 지표성분 dieckol의 분석법 검증)

  • Xu, Yan;Kim, Eun Suh;Lee, Ji-Soo;Kim, Gun-Hee;Lee, Hyeon Gyu
    • Korean Journal of Food Science and Technology
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    • 제51권5호
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    • pp.420-424
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    • 2019
  • An HPLC analysis method was developed and standardized for the detection of dieckol as a functional food ingredient in Ecklonia cava extracts. HPLC was performed using a Capcell Pak C18 column ($250{\times}4.6mm$, $5{\mu}m$) with a gradient elution of water and acetonitrile, both containing 0.1% (v/v) trifluoroacetic acid, at a flow rate of 1.0 mL/min at $25^{\circ}C$. The eluate was detected at 230 nm. For validation, the specificity, linearity, accuracy, precision, limit of detection (LOD), and limit of quantification (LOQ) of dieckol were measured. The calibration curve for the detection of dieckol had high linearity ($R^2=0.9994$), with LOD and LOQ values of 0.38 and $1.16{\mu}g/mL$, respectively. Recovery of the quantified compound ranged from 99.61 to 100.71%. The relative standard deviation values of the intra-day and inter-day precisions were less than 1.7 and 1.25%, respectively. These results indicate that the reported HPLC method is simple, reliable, and reproducible for the detection of dieckol in Ecklonia cava extracts.

A novel approach for rice straw agricultural waste utilization: Synthesis of solid aluminosilicate matrices for cesium immobilization

  • Panasenko, A.E.;Shichalin, O.O.;Yarusova, S.B.;Ivanets, A.I.;Belov, A.A.;Dran'kov, A.N.;Azon, S.A.;Fedorets, A.N.;Buravlev, I. Yu;Mayorov, V. Yu;Shlyk, D. Kh;Buravleva, A.A.;Merkulov, E.B.;Zarubina, N.V.;Papynov, E.K.
    • Nuclear Engineering and Technology
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    • 제54권9호
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    • pp.3250-3259
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    • 2022
  • A new approach to the use of rice straw as a difficult-to-recycle agricultural waste was proposed. Potassium aluminosilicate was obtained by spark plasma sintering as an effective material for subsequent immobilization of 137Cs into a solid-state matrix. The sorption properties of potassium aluminosilicate to 137Cs from aqueous solutions were studied. The effect of the synthesis temperature on the phase composition, microstructure, and rate of cesium leaching from samples obtained at 800-1000 ℃ and a pressure of 25 MPa was investigated. It was shown that the positive dynamics of compaction was characteristic of glass ceramics throughout the sintering. Glass ceramics RS-(K,Cs)AlSi3O8 obtained by the SPS method at 1000 ℃ for 5 min was characterized by a high density of ~2.62 g/cm3, Vickers hardness ~ 2.1 GPa, compressive strength ~231.3 MPa and the rate of cesium ions leaching of ~1.37 × 10-7 g cm-2·day-1. The proposed approach makes it possible to safe dispose of rice straw and reduce emissions into the atmosphere of microdisperse amorphous silica, which is formed during its combustion and causes respiratory diseases, including cancer. In addition, the obtained is perspective to solve the problem of recycling long-lived 137Cs radionuclides formed during the operation of nuclear power plants into solid-state matrices.