• Title/Summary/Keyword: AP-1

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Modulation of Activator Protein-1 (AP-1) and MAPK Pathway by Flavonoids in Human Prostate Cancer PC3 Cells

  • Gopalakrishnan, Avanthika;Xu, Chang-Jiang;Nair, Sujit S.;Chen, Chi;Hebbar, Vidya;Kong, Ah-Ng Tony
    • Archives of Pharmacal Research
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    • v.29 no.8
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    • pp.633-644
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    • 2006
  • In last couple of decades the use of natural compounds like flavonoids as chemopreventive agents has gained much attention. Our current study focuses on identifying chemopreventive flavonoids and their mechanism of action on human prostate cancer cells. Human prostate cancer cells (PC3), stably transfected with activator protein 1 (AP-1) luciferase reporter gene were treated with four main classes of flavonoids namely flavonols, flavones, flavonones, and isoflavones. The maximum AP-1 luciferase induction of about 3 fold over control was observed with $20\;{\mu}M$ concentrations of quercetin, chrysin and genistein and $50\;{\mu}M$ concentration of kaempferol. At higher concentrations, most of the flavonoids demonstrated inhibition of AP-1 activity. The MTS assay for cell viability at 24 h showed that even at a very high concentration $(500\;{\mu}M)$, cell death was minimal for most of the flavonoids. To determine the role of MAPK pathway in the induction of AP-1 by flavonoids, Western blot of phospho MAPK proteins was performed. Four out of the eight flavonoids namely kaempferol, apigenin, genistein and naringenin were used for the Western Blot analysis. Induction of phospho-JNK and phospho-ERK activity was observed after two hour incubation of PC3-AP1 cells with flavonoids. However no induction of phospho-p38 activity was observed. Furthermore, pretreating the cells with specific inhibitors of JNK reduced the AP-1 luciferase activity that was induced by genistein while pretreatment with MEK inhibitor reduced the AP-1 luciferase activity induced by kaempferol. The pharmacological inhibitors did not affect the AP-1 luciferase activity induced by apigenin and naringenin. These results suggest the possible involvement of JNK pathway in genistein induced AP-1 activity while the ERK pathway seems to play an important role in kaempferol induced AP-1 activity.

Preparation of Spherical Energetic Composites by Crystallization/Agglomeration and their Thermal Decomposition Characteristics (결정화/응집 기법에 의한 구형 에너지 복합체 제조 및 그 열분해 특성)

  • Lee, Eun-Ae;Shim, Hong-Min;Kim, Jae-Kyeong;Kim, Hyoun-Soo;Koo, Kee-Kahb
    • Applied Chemistry for Engineering
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    • v.27 no.2
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    • pp.158-164
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    • 2016
  • Spherical DADNE/AP (1,1-diamino-2,2-dinitroethylen/ammonium perchlorate) energetic composites were produced by drowning-out/agglomeration (D/A). The agglomeration of DADNE with AP particles was found to be affected by the amount of the bridging liquid, stirring velocity and residence time. The composites appeared to grow dramatically with the amount of bridging liquid which triggers agglomeration. As the stirring velocity and the residence time increased, the size of composites increased and then tended to decrease. Thermal gravimetric analysis showed that the addition of DADNE activates the low temperature decomposition (LTD) of AP. For the neat AP, the only about 30 wt% of AP was found to decompose at the LTD. On the other hand, it was found that 70 wt% of AP decomposed when DADNE was added by physical mixing and 90 wt% of AP decomposed when the DADNE/AP composites were prepared by the D/A method.

A Study on Clinical Classification and Characteristic of Children with Recurrent Abdominal Pain (만성(慢性) 반복성(反復性) 복통(腹痛)을 주증(主症)으로 하는 환아(患兒)의 임상적(臨床的) 특징(特徵)에 관한 연구(硏究) -기능성 복통을 중심으로-)

  • Kim, Sung-Hee;Park, Sang-Wook;Lee, Seung-Yeon
    • The Journal of Pediatrics of Korean Medicine
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    • v.16 no.2
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    • pp.1-22
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    • 2002
  • Purpose : This Study was conducted to evaluate clinical characteristic of children with recurrent abdominal pain (RAP) and to be classified by its six subtype in the Oriental Pediatric Text Book and to find out relationship of western classification. Methods : Patients who visited Dong-Eui Oriental Medical hospital from August, 2001 to October, 2002 due to RAP were included. According to questionnaire and history taking, RAP was classified by its six subtype based on Oriental medical theory. Results : 1. Patients with RAP were more internalized, have a close relation with their parents, and have strong desires of success, but social intercourse is low. 2. 76% of Patients have a less desire to eat and 67% of Patients have a diarrhea or constipation. 3. According to questionnaire, first abdominal pain was their $3{\sim}5$ ages most, cause of occurrence was more 'eating cold foods' most, time of AP (abdominal pain) was $1{\sim}2$ hours after eating and no characteristic most, site of AP was the umbilicus most, shape of AP was impotent pain most, cause of reduce pain was abdominal massage and defection most. 4. frequency of RAP's type, AP caused by diet(食積腹痛) is 45.5%, AP caused by cold(寒腹痛) is 29.1%, AP caused by cold in internal organs of deficiency(臟腑虛冷腹痛) is 12.7%, stagnation of qi and stasis of blood(氣滯血瘀腹痛) is 10.9%, AP caused by internal diet and external cold(內食外寒腹痛) is 1.8%. There is no AP caused by parasites(蟲腹痛). 5. During clinical classifications of RAP, cause of occurrence was most important cause of reduce pian, defection practice was helpful for diagnosis, but shape of AP, site of AP was not helpful. 6. With relationship of Oriental classification and western classification, AP caused by diet is similar to dysmotilitylike dyspepsia and irriTable bowel syndrome. AP caused by cold is similar to irriTable bowel syndrome. AP caused by cold in internal organs of deficiency is similar to unspecified dyspepsia. stagnation of qi and stasis of blood and AP caused by internal diet and external cold is not like to western classification. Conclusion : RAP in Childhood is most occurred by food and cold. there is few AP caused by stagnation of qi and stasis of blood and internal diet and external cold. So the study on subclassification and clinical Manifestations of RAP in Childhood is more performed.

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Purification and characterization of a 1,3-β-D-glucan recognition protein from Antheraea pernyi larve that is regulated after a specific immune challenge

  • Youlei, Ma;Jinghai, Zhang;Yuntao, Zhang;Jiaoshu, Lin;Tianyi, Wang;Chunfu, Wu;Rong, Zhang
    • BMB Reports
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    • v.46 no.5
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    • pp.264-269
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    • 2013
  • Pattern recognition receptors are known to participate in the activation of Prophenoloxidase system. In this study, a 1,3-${\beta}$-D-glucan recognition protein was detected for the first time in Antheraea pernyi larvae (Ap-${\beta}GRP$). Ap-${\beta}GRP$ was purified to 99.9% homogeneity from the hemolymph using traditional chromatographic methods. Ap-${\beta}GRP$ specifically bind 1,3-${\beta}$-D-glucan and yeast, but not E. coli or M. luteus. The 1,3-${\beta}$-D-glucan dependent phenoloxidase (PO) activity of the hemolymph inhibited by anti-Ap-${\beta}GRP$ antibody could be recovered by addition of purified Ap-${\beta}GRP$. These results demonstrate that Ap-${\beta}GRP$ acts as a biosensor of 1,3-${\beta}$-Dglucan to trigger the Prophenoloxidase system. A trace mount of 1,3-${\beta}$-D-glucan or Ap-${\beta}GRP$ alone was unable to trigger the proPO system, but they both did. Ap-${\beta}GRP$ was specifically degraded following the activation of proPO with 1,3-${\beta}$-Dglucan. These results indicate the variation in the amount of Ap-${\beta}GRP$ after specific immune challenge in A. pernyi hemolymph is an important regulation mechanism to immune response.

Mobile Malicious AP Detection and Cut-off Mechanism based in Authentication Network (인증 네트워크 상의 비 인가된 모바일 AP 탐지 및 차단 기법)

  • Lim, Jae-Wan;Jang, Jong-Deok;Yoon, Chang-Pyo;Ryu, Hwang-Bin
    • Convergence Security Journal
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    • v.12 no.1
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    • pp.55-61
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    • 2012
  • Owing to the development of wireless infrastructure and mobile communication technology, There is growing interest in smart phone using it. The resulting popularity of smart phone has increased the Mobile Malicious AP-related security threat and the access to the wireless AP(Access Point) using Wi-Fi. mobile AP mechanism is the use of a mobile device with Internet access such as 3G cellular service to serve as an Internet gateway or access point for other devices. Within the enterprise, the use of mobile AP mechanism made corporate information management difficult owing to use wireless system that is impossible to wire packet monitoring. In this thesis, we propose mobile AP mechanism-based mobile malicious AP detection and prevention mechanism in radius authentication server network. Detection approach detects mobile AP mechanism-based mobile malicious AP by sniffing the beacon frame and analyzing the difference between an authorized AP and a mobile AP mechanism-based mobile malicious AP detection.

Quantification of 2-Acetyl-1-pyrroline from the Aroma Rice Germplasm by Gas Chromatography (Gas chromatography를 이용한 향미 유전자원의 2-acetyl-1-pyrroline 정량분석)

  • Kim, Jeong-Soon;Park, One-Sung;Ahn, Sang-Nag;Lee, Jung-Ro;Gwag, Jae-Gyun;Kim, Tae-San;Lee, Sok-Young
    • Korean Journal of Food Science and Technology
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    • v.40 no.5
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    • pp.516-521
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    • 2008
  • This study was conducted to optimize the analysis condition and quantify the 2-acetyl-1-pyrroline (2AP) in the brown aroma rice. Extraction effect of the solvent for 2AP was the order of ethanol>acetonitrile>methanol in the range from 30 to $90^{\circ}C$. In the extraction time of 15, 30, 60, and 90 min, the 30 min had the highest 2AP concentration, and it was decreased according to lapse of time. At grinding time, 5 sec resulted in highest 2AP concentration. It was recommended that five sec grinding time, using ethanol, at $90^{\circ}C$ for 30 min was the optimization conditions to quantify the 2AP. Hyangmibyeo2ho and Aranghyangchalbyeo were mild aroma. In the foreign aroma rice, 11 of 19 accessions of Indica types and 2 of 6 accessions of Japonica types were more than mild aroma. Finally, 30 accessions of aroma rice were selected based on their 2AP concentration and agronomic traits.

Transcriptional Regulation of the Murine Dopamine Receptor Regulating Factor (DRRF) Gene (생쥐 도파민 수용쳬 조절인자 (DRRF) 유전자의 전사조절)

  • Kim Ok Soo;Lee Young-Choon;Lee Sang-Hyeon
    • Journal of Life Science
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    • v.15 no.1 s.68
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    • pp.55-60
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    • 2005
  • The murine dopamine receptor regulating factor (DRRF) gene is transcribed from a TATA-less promoter that has several putative Sp1 binding sites. The present investigation identifies functional transcription factors that modulate the expression of this gene, In the $D_2-expressing$ NB41A3 cells, Spl potently activates transcription from the DRRF promoter in pCAT-DRRF-1153/+17, but DRRF effectively inhibits it. Deletion of the 31 bp fragment between -1153 and -1122 decreased transcription down to about $60\%$. This fragment contains a functional API binding site. In addition, deletion of the 129 bp region between -901 and -772 further decreased transcription. The latter region has a functional AP2 binding site. Using a DRRF_AP1 (bases -1153 to -1121) probe, a specific retarded band was observed, and the unlabeled AP1 consensus competitor could effectively compete away this retarded band. In addition, using a DRRF_AP2 (bases -873 to -846), a specific retarded band was observed, and the unlabeled AP2 consensus competitor could effectively compete away this retarded band. The present observations suggest that Spl and DRRF regulate the DRRF promoter and that both API and AP2 also modulate this gene.

Antioxidant and Alcohol Dehydrogenase Activity of Water Extracts From Abalone Containing Medicinal Plants (전복과 천연 식물류 복합물의 항산화 및 알코올대사 효소 활성)

  • Shin, Jung-Hye;Lee, Soo-Jung;Choi, Duk-Ju;Kang, Min-Jung;Sung, Nak-Ju
    • Korean journal of food and cookery science
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    • v.24 no.2
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    • pp.182-187
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    • 2008
  • This study was performed to evaluate the possible production of a functional beverage derived from abalone and with several plants (AP). Five types of AP composites were prepared having different medicinal plant compositions (AP- I : abalone, garlic, jujube, Chinese matrimony fruit, dried orange peel, licorice root, zinger, honey; AP- II: abalone, garlic, black bean, Chinese matrimony fruit, cornus fruit, licorice root, zinger, honey; AP-III : abalone, jujube, arrow root, milk vetch, dried orange peel, licorice root, zinger, honey; AP-N: abalone, black bean, arrow root, milk vetch, cornus fruit, liquorice root, zinger, honey; AP-V : abalone, garlic, Chinese matrimony fruit, milk vetch, licorice root, zinger, honey). In vitro analysis were performed to examine the antioxidant contents and alcohol dehydrogenase activities of the composites. AP- II had the highest total phenol contents ($28.55{\pm}1.56\;mg/l00\;g$), and AP- V the highest level of flavonoids ($47.61{\pm}1.58\;mg/l00\;g$). At $78.89{\pm}0.16%$, AP- V displayed the strongest electron donating ability followed by AP-II($57.99{\pm}0.21%$) and API ($37.66{\pm}0.20%$). Reducing power was also significantly higher in AP- V. The Hydroxyl radical scavenging and SOD-like activities of all composites were less than 15% and 20%, respectively. At 12.5% alcohol concentration, ADH activity ranged from $114.47{\pm}2.18{\sim}121.39{\pm}4.36%$ and ALDH activity ranged from $100.04{\pm}2.90{\sim}129.54{\pm}4.80%$; AP- I , AP- II, and AP- Vin 12.5% of alcoholic concentration. The composites of AP- I , AP- II, and AP- V, all containing garlic and Chinese matrimony fruit, were significantly stronger than AP-III and AP-N. Finally, also at 12.5% alcoholic concentration, the ALDH activity of AP- V was higher than its ADH activity.

A Study of Call Admission Scheme using Power Strength Threshold value between APs in Wireless LAN Environments (무선랜 환경에서 AP간 전력임계치값을 통한 호 처리 연구)

  • Lim, Seung-Cheol
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.15 no.3
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    • pp.107-112
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    • 2015
  • A smart phone and the number of wireless terminals are mobile-to the nature of the AP mobility are many call transfer between wireless terminals and AP (Access Point). Each wireless terminal that is the call processing process for each is relatively large to cause the wireless traffic, and a factor that inhibits the efficient use of the radio band on the AP. In this paper, we use the power intensity threshold and threshold timer of the AP received by the mobile station to reduce the amount of switching traffic between the AP's cause and traffic generation factors between the wireless device and the AP that can effectively utilize the radio traffic from the AP the measures proposed. The proposed method and the conventional method is improved by simulation to handle the amount of radio traffic from the AP it was confirmed that it is possible to effectively utilize the whole of the radio band.

THE INTEGRALS OF BANACH SPACE-VALUED FUNCTIONS

  • Park, Jae Myung;Lee, Deok Ho;Yoon, Ju Han
    • Journal of the Chungcheong Mathematical Society
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    • v.21 no.1
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    • pp.79-89
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    • 2008
  • In this paper, we define the ap-Henstock integral and the ap-Denjoy integral of Banach-valued functions, and we investigate some properties of these two integrals. In particular, we show that the ap-Henstock integral is equivalent to the ap-Denjoy integral.

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