• Journal of Animal Science and Technology
• /
• v.64 no.3
• /
• pp.481-499
• /
• 2022

This study aims to determine the effects of D-methionine (D-Met) isomer and the methionine precursor 2-hydroxy-4-methylthiobutanoic acid i (HMBi) supplementation on milk protein synthesis on immortalized bovine mammary epithelial cell (MAC-T). MAC-T cells were seeded using 10-cm dishes and cultured in Dulbecco's modified Eagle's medium/F12 (DMEM/F12) basic medium. The basic medium of DMEM/F12 was replaced with the lactogenic DMEM/ F12 differentiation medium when 90% of MAC-T cells reached confluency. The best dosage at 0.6 mM of D-Met and HMBi and incubation time at 72 h were used uniformly for all treatments. Each treatment was replicated six times wherein treatments were randomly assigned in a 6-well plate. Cell, medium, and total protein were determined using a bicinchoninic acid protein assay kit. Genes, proteomics and metabolomics analyses were also done to determine the mechanism of the milk protein synthesis pathway. Data were analyzed by two-way analysis of variance (ANOVA) with supplement type and plate as fixed effects. The least significant difference test was used to evaluate the differences among treatments. The HMBi treatment group had the highest beta-casein and S6 kinase beta-1 (S6K1) mRNA gene expression levels. HMBi and D-Met treatments have higher gene expressions compared to the control group. In terms of medium protein content, HMBi had a higher medium protein quantity than the control although not significantly different from the D-Met group. HMBi supplementation stimulated the production of eukaryotic translation initiation factor 3 subunit protein essential for protein translation initiation resulting in higher medium protein synthesis in the HMBi group than in the control group. The protein pathway analysis results showed that the D-Met group stimulated fructose-galactose metabolism, glycolysis pathway, phosphoinositide 3 kinase, and pyruvate metabolism. The HMBi group stimulated the pentose phosphate and glycolysis pathways. Metabolite analysis revealed that the D-Met treatment group increased seven metabolites and decreased uridine monophosphate (UMP) production. HMBi supplementation increased the production of three metabolites and decreased UMP and N-acetyl-L-glutamate production. Taken together, D-Met and HMBi supplementation are effective in stimulating milk protein synthesis in MAC-T cells by genes, proteins, and metabolites stimulation linked to milk protein synthesis.

• 한국노년학
• /
• v.29 no.3
• /
• pp.1159-1182
• /
• 2009

The purpose of this study is to examine everyday emotional experience of urban elderly in Korea. Specifically, this study explores gender differences in the effect of place/location of activity and companionship on the daily emotional experience. Data were collected from 148 urban elderly (men=76, women=72) in Seoul, using Experience Sampling Method(ESM). The unit of analysis was the self-report and in total 5,530 self-reports were provided. The statistical methods used for the data analyses were descriptive statistics, t-test, χ² verification, and ANOVA. The major findings are as follows. First, the elderly in Korea spend a quite substantial portion of their time at home, and they spend the largest share of their time in company with their spouse. Second, women report higher intensity of stress and irritation than men in terms of overall emotional experience. Third, the effect of activity location and companionship on emotional experience turned out to be different depending on gender. Emotional experience of daily lives and overall quality of life are shown to be inter-related. Based on these findings, this study provides a significant implication that situational contexts of experience should be considered in order to understand the daily emotional experience of the contemporary Korean elderly.

• The Journal of Advanced Prosthodontics
• /
• v.16 no.2
• /
• pp.126-138
• /
• 2024

PURPOSE. The aim of this study was to evaluate the influence of different 3D dental resins, using a manufacturer recommended printer and a third-party printer, on cellular responses of human gingival cells. MATERIALS AND METHODS. Three NextDent resins (Denture 3D+, C&B MFH and Crowntec) were used to produce specimens on printers NextDent 5100 (groups ND, NC and NT, respectively) and Phrozen Sonic Mini 4K (groups PD, PC and PT, respectively). Human gingival fibroblasts were cultured and biocompatibility was evaluated on days 1, 3 and 7. IL-6 and IL-8 concentrations were evaluated at 3 days using ELISA. Surface roughness was evaluated by a contact profilometer. SEM and fluorescence micrographs were analyzed at days 1 and 7. Statistical analyses were performed using SPSS and mean differences were tested using ANOVA and post-hoc Tukey tests (P < .05). RESULTS. There was an increase in cellular viability after 7 days in groups PC and PT, when compared to group PD. ND group resulted in higher concentration of IL-6 when compared to PT group. SEM and fluorescence micrographs showed less adhesion and thinner morphology of fibroblasts from group PD. No significant differences were found regarding surface roughness. CONCLUSION. The use of different printers or resins did not seem to influence surface roughness. NextDent 5100 and Phrozen Sonic Mini 4K produced resins with similar cellular responses in human gingival fibroblasts. However, Denture 3D+ resin resulted in significantly lower biocompatibility, when compared to C&B MFH and Crowntec resins. Further testing is required to support its long-term use, required for complete dentures.

• Korean Journal of Radiology
• /
• v.20 no.4
• /
• pp.683-692
• /
• 2019

Objective: We aimed to evaluate correlations between computed tomography (CT) parameters and pulmonary function test (PFT) parameters according to disease severity in patients with chronic obstructive pulmonary disease (COPD), and to determine whether CT parameters can be used to predict PFT indices. Materials and Methods: A total of 370 patients with COPD were grouped based on disease severity according to the Global Initiative for Chronic Obstructive Lung Disease (GOLD) I-IV criteria. Emphysema index (EI), air-trapping index, and airway parameters such as the square root of wall area of a hypothetical airway with an internal perimeter of 10 mm (Pi10) were measured using automatic segmentation software. Clinical characteristics including PFT results and quantitative CT parameters according to GOLD criteria were compared using ANOVA. The correlations between CT parameters and PFT indices, including the ratio of forced expiratory volume in one second to forced vital capacity (FEV1/FVC) and FEV₁, were assessed. To evaluate whether CT parameters can be used to predict PFT indices, multiple linear regression analyses were performed for all patients, Group 1 (GOLD I and II), and Group 2 (GOLD III and IV). Results: Pulmonary function deteriorated with increase in disease severity according to the GOLD criteria (p < 0.001). Parenchymal attenuation parameters were significantly worse in patients with higher GOLD stages (P < 0.001), and Pi10 was highest for patients with GOLD III (4.41 ± 0.94 mm). Airway parameters were nonlinearly correlated with PFT results, and Pi10 demonstrated mild correlation with FEV1/FVC in patients with GOLD II and III (r = 0.16, p = 0.06 and r = 0.21, p = 0.04, respectively). Parenchymal attenuation parameters, airway parameters, EI, and Pi10 were identified as predictors of FEV₁/FVC for the entire study sample and for Group 1 (R² = 0.38 and 0.22, respectively; p < 0.001). However, only parenchymal attenuation parameter, EI, was identified as a predictor of FEV₁/FVC for Group 2 (R² = 0.37, p < 0.001). Similar results were obtained for FEV₁. Conclusion: Airway and parenchymal attenuation parameters are independent predictors of pulmonary function in patients with mild COPD, whereas parenchymal attenuation parameters are dominant independent predictors of pulmonary function in patients with severe COPD.

• Journal of Life Science
• /
• v.23 no.6
• /
• pp.796-803
• /
• 2013

Property changes and bacterial characterizations by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) were investigated during the fermentation of Makgeollies by 5 isolated yeast strains. Changes of pH were large between day 0 (pH 6) and day 2 (pH 3) and showed less variation after then. ANOVA analyses revealed that pHs were statistically different with fermentation times (p<0.001), while strains (p=0.60) did not. Acidities were changed from 0.19 to 1.04% and showed rather high increase from day 2, and fermentation times (p<0.001) and strains (p=0.006) represented statistical differences. All strains showed less than 0.150% at amino-type nitrogen contents except S strain showed 0.442% at day 8, and there were no statistical differences with fermentation times (p=0.4558) and strains (p=0.3513). Saccharinities of C strain were higher from day 4, and fermentation times (p<0.0001) and strains (p=0.007) showed statistical differences. Large variation of alcohol concentrations (%) were observed between day 0 (0%) and day 2 (10%) and showed less variation after day 2, and there was no statistical difference with strains. Dominant prokaryotes were Lactobacillus fermentum and Pediococcus pentosaceus, which producing acids and functional materials. Dominant eukaryote was Saccharomyces cerevisiae, which might be resulted from addition of yeasts.

• The Journal of Small Business Innovation
• /
• v.19 no.2
• /
• pp.53-68
• /
• 2016

Motivation and activities for technological learning, entrepreneurship, innovation, and creativity are driving forces of economic development in Asian countries. In the early stages of technological development, technological learning and entrepreneurship are efficient ways in which to catch up with advanced countries because firms can accumulate skills and knowledge quickly at relatively low risk. In the later stages of technological development, however, innovation and creativity become more important. This study aims to identify a) the factors (learning capabilities) that influence technological learning performance and b) barriers to enhancing innovation capabilities for the creative economy and organizations. The major part of this study is related to learning capabilities in the post-catch-up era. Based on a literature review and observations from Korean experiences, this study proposes a technological learning model composed of various influencing factors on technological learning. Three hypotheses are derived, and data are collected from Korean machine tool manufacturers. Intense interviews with CEOs and R&D directors are conducted using structured questionnaires. Statistical analysis, such as correlation and ANOVA are then carried out. Furthermore, this study addresses how to enhance innovation capabilities to move forward. Innovation enablers and barriers are identified by case studies and policy analysis. The results of the empirical study identify several levels of firms' learning capabilities and activities such as a) stock of technology, b) potential of technical labor, c) explicit technological efforts, d) readiness to learn, e) top management support, f) a formal technological learning system, g) high learning motivation, h) appropriate technology choice, and i) specific goal setting. These learning capabilities determine firms' learning performance, especially in the early stages of development. Furthermore, it is found that the critical factors for successful technological learning vary along the stages of technology development. Throughout the statistical and policy analyses, this study confirms that technological learning can be understood as an intrinsic principle of the technology development process. Firms perform proactive and creative learning in the late stages, while reactive and imitative learning prevails in the early stages. In addition, this study identifies the driving forces or facilitating factors enhancing innovation performance in the post catch-up era. The results of the preliminary case studies and policy analysis show some facilitating factors such as a) the strategic intent of the CEO and corporate culture, b) leadership and change agents, c) design principles and routines, d) ecosystem and collaboration with partners, and e) intensive R&D investment.

• The Journal of the Korea Contents Association
• /
• v.16 no.8
• /
• pp.292-309
• /
• 2016

The purpose of the present paper is to investigate the effects of stress and depression experienced by the first (1955-1964) and second (1968-1974) generations of baby boomers on their psychological well-being as well as the moderation effects of social supports for them. For this purpose, 369 who come under the category of the first- and second-generation baby boomers were selected by convenient sampling from among the participants in the programs of the life-long educational institutes in Seoul metropolitan area and questionnaires of self-administered type were distributed to them. Frequency analysis, t-test, ANOVA, correlation analysis and hierarchial regression analysis which used input of mean-centered variables and interaction term were conducted to determine the moderating effects of social supports based on the replies to the questionnaires. The major outcomes of the analyses could be summed up as follows: first, the stress and depression suffered by the first and second generations of baby boomers turned out to have negative (-) influence upon their psychological well-being; second, the social supports for those two generations were found to exercise positive (+) effects upon their psychological well-being; and third, a survey of difference in the moderating effects of social supports between the first- and second-generation baby boomers showed that, in case of the first generation, the worse their economic status and the higher their stress and depression were, the lower their psychological well-being tended to be and that social supports functioned to hike their psychological well-being but had moderating effects only in connection with stress. In case of the second generation, however, it was shown that the higher their stress and depression got, the lower their psychological well-being developed and that social supports might increase their psychological well-being but without any moderating effects on the part of relationship with their stress and depression. A practical and political method was discussed to improve baby boomers mental health.

• Sleep Medicine and Psychophysiology
• /
• v.15 no.2
• /
• pp.87-93
• /
• 2008

Introduction: Although it is well known that medical students are not getting an adequate amount of sleep, there have been only few studies on the sleep patterns of medical students in Korea. Therefore, the present study aimed to investigate the life style and sleep patterns of Korean medical students and the impact they have on the students' academic performance. Methods: A questionnaire package was administered to the $3^{rd}$ year medical students at the Seoul National University to examine their sleep patterns on weekdays and weekends. It consisted of questions asking about their lifestyles as well as Pittsburgh sleep quality index (PSQI) and GPA (Grade Point Average) that are considered relevant to their sleep patterns. A total of 110 students (85 males and 25 females, mean age $24.4{\pm}20.6$) responded to the survey and the result was analyzed using the independent t-test, the chi-square test, the paired t-test, Pearson's rank correlation and ANOVA. P-values of less than 0.05 were considered statistically significant in all analyses. Results: The weekend bedtime was significantly delayed (01：24 on weekday；03：12 on weekend; t=-5.23, p<0.01), the weekend rise time was delayed (07：36 on weekday；10：30 on weekend ; t=-24.48, p<0.01) and the total sleep time was increased on weekends (5：57 on weekday；8：17 on weekend ; t=15.94, p<0.01). They wished to sleep for 7 hours 6 minutes which was different from their actual weekday total sleep time (t=-11.41, p<0.01). The poor sleeper group had lower GPAs than the good sleeper group (t=2.05, p<0.05). The GPA of medical students were negatively correlated with age (r=-0.23, p<0.05), daily amount of smoking (r=-0.78, p<0.01), total amount of smoking (r=-0.75, p<0.01), weekday sleep latency (r=-0.23, p<0.05), weekend sleep latency (r=-0.23, p<0.05) and PSQI score (r=-0.30, p<0.01). Conclusion: Medical students were experiencing a lack of sleep during weekdays as they have a later bedtime and earlier rise time, and consequently had more hours of sleep on weekends. Overall, the responded students were experiencing poor sleep quality, and the GPAs of the poor sleeper group were lower than those of the good sleeper group.

• Journal of Korean Home Economics Education Association
• /
• v.29 no.4
• /
• pp.1-14
• /
• 2017

This study described the status of family dining and personality of adolescents, determined the differences in family dining according to personal variables, and examined the relationship between family dining and personality in adolescence. The Data were collected from self-reported inventory of the middle and high school students in a city and 1,259 data copies were used for analyses. Data were analyzed for frequency, percentage, mean, standard deviation, Cronbach's ${\alpha}$, t-test, ANOVA, scheffe test, and multiple regression analysis using SPSS/PC 18.0 program. The results obtained were as follows. First, as for the weekly family dining frequency, more than 7 times had the most number of responses(44.2%), followed by 1~2 times(20.8%), 3~4 times(18.1%), 5~6 times(12.9%). The average scores for family dining perception and family dining attitudes were 3.75 and 3.42 on a 5-Likert scale, respectively, which means moderately high. But, family dining rules was 2.80, which means moderate. Agreeableness was the highest(3.68) among the personality components followed by Openness/intellect(3.42), Extraversion(3.33), Conscientiousness(3.14), and Emotional Stability(3.05) out of 5-point Likert scale. Second, there were statistically significant differences in family dining frequency, family dining perception, family dining rules, and family dining attitudes depending on the gender, school level, and the father's academic attainment. Third, family dining frequency, family dining perception, and family dining attitudes had statistically significant influence on the personality of adolescents after personal variables were considered. But, there was no significant relationship between family dining rules and the personality. The outcomes indicate that family dining affects the personality of adolescents. School needs to offer quality programs with various teaching methods to enhance family dining frequency, family dining perception, and family dining attitudes.

• Journal of Korean Home Economics Education Association
• /
• v.29 no.4
• /
• pp.31-47
• /
• 2017

The purpose of this study was to evaluate the effectiveness of parenting education program for adolescents. Parenting education program for adolescents was developed and implemented at three secondary schools in Seoul, Chungcheong province, and Gwangju metropolitan city with focus on the following topics respectively 'parent-child communication', 'understanding fatherhood', and 'preparation for parenting based on self-understanding'. Data were collected from self-reported inventory and 97 data copies for pre- and post tests were used for analyses. Data were analyzed for frequency, percentage, paired t-test and One way ANOVA using SPSS/PC 21.0 program. The results from mean comparison of pre- and post tests were as follows. First, perception of necessity for parenting education and needs for including parenting program into regular secondary school curriculum increased statistically, which means that parenting program must be implemented regularly to meet the demands of adolescents for parenting program. Second, the main reason for not having children was 'burden for rearing children', which means that enough child-care skill and information need to be included. Third, the mean difference between pre- and post tests regarding perception of performing parents' roles was statistically significant, which means that the participants' perception of performing parents' roles became positive. Fourth, the 32 high school students participating in parenting education of 'understanding fatherhood' did not have significant difference in gender role. The 29 middle school students participating in parenting education of 'parent-child communication' had significant difference in perception of communication. The 36 high school students participating in parenting education of 'preparation for parenting based on self-understanding' had significant difference in self-esteem. This results identified the effectiveness of parenting education for adolescents. Therefore, Home Economics subject must be selected to give the opportunity of parenting education to high school students.