• Title/Summary/Keyword: AH36

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Suppression of Migration and Invasion by Alnus hirsuta in Human Hepatocellular Carcinoma Cells

  • Bo-Ram Kim;Su Hui Seong;Tae-Su Kim;Jin-Ho Kim;Chan Seo;Ha-Nul Lee;Sua Im;Jung Eun Kim;Ji Min Jung;Jung Up Park;Kyung-Min Choi;Jin-Woo Jeong
    • Korean Journal of Plant Resources
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    • v.36 no.3
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    • pp.207-218
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    • 2023
  • Hepatocellular carcinoma (HCC) has a poor prognosis and high metastasis and recurrence rates. Although extracts of Alnus hirsuta (Turcz. ex Spach) Rupr. (AH) have been demonstrated to possess potential anti-inflammatory and anti-cancer activities, the underlying mechanism of AH in HCC treatment remains to be elucidated. We investigated the effects and potential mechanisms of AH on migration and invasion of Hep3B cells. Within the non-cytotoxic concentration range, AH significantly inhibited motility and invasiveness of Hep3B cells in a concentration-dependent manner. Inhibitory effects of AH on cell invasiveness are associated with tightening of tight junctions (TJs), as demonstrated by an increase in transepithelial electrical resistance. Immunoblotting indicated that AH decreased levels of claudins, which form major components of TJs and play key roles in the control and selectivity of paracellular transport. Furthermore, AH inhibited the expression and activity of matrix metalloproteinase (MMP)-2 and MMP-9 and simultaneously increased the levels of tissue inhibitor of metalloproteinase (TIMP)-1 and TIMP-2. These effects were related to inactivation of the phosphoinositide 3-kinase (PI3K)/AKT pathway in Hep3B cells. Therefore, AH inhibits migration and invasion of Hep3B cells by inhibiting the activity of MMPs and tightening TJs through suppression of claudin expression, possibly by suppressing the PI3K/AKT signaling pathway.

Butt Weldability of Shipbuilding Steel AH36 Using Laser-Arc Hybrid Welding (조선용 강판 AH36의 레이저-아크 하이브리드 용접시 맞대기 용접 특성)

  • Kim, Jong Do;Myoung, Gi Hoon;Suh, Jung
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.40 no.10
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    • pp.901-906
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    • 2016
  • The purpose of this study is to improve productivity by implementing one-pass full penetration welding using laser-arc hybrid welding for AH36. On increasing the thickness of the plate, a higher power of laser and arc was required to obtain full penetration. However, increasing the power of heat source caused undercut defects at both ends of the bead. This undercut was prevented by controlling the parameters of welding voltage and pulse correction. Hardness measurement and tensile test were conducted to apprehend the mechanical properties of weld. Also, by carrying out the microstructure observation for laser and arc regions, microstructural properties were understood.

A Study on Effect of PWHT in AH36-TMCP Steel (AH36-TMCP강의 용접후열처리 효과에 관한 연구)

  • 유효선;장원상;안병국;정세희
    • Journal of Welding and Joining
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    • v.16 no.6
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    • pp.44-51
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    • 1998
  • It is well known that the fine bainitic microstructure obtained by TMCP(thermo-mechanical control process) secures the high toughness of base metal. Besides, TMCP steel is very suitable for high heat input in welding as it has low carbon equivalent. In HAZ, however, the accelerated cooling effect imparted on the matrix by the weld thermal cycles is relieved and thus the weldment of TMCP steel has softening zone which shows low fracture toughness compared with base metal. Therefore, PHWT of weldment is carried out to improve the fracture toughness in weldment of TMCP steel which has softening zone. In this study, the effects of PWHT on the weldment of AH36-TMCP steel are investigated by the small punch (SP) test. From the several results such as SP energy and displacement at room temperature, the behavior of transition curves, the fracture strength at -196$^{\circ}C$, distribution of (DBTT)sp and (DBTT)sp, the PWHT condition of A.C. after 85$0^{\circ}C$-1 sec W.C. was suitable condition for recovering a softening zone of HAZ as welded.

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Structural Identification of $Siderophore_{AH18}$ from Bacillus subtilis AH18, a Biocontrol agent of Phytophthora Blight Disease in Red-pepper (Bacillus subtilis AH18의 고추역병 방제능과 $Siderophore_{AH18}$의 구조분석)

  • Woo, Sang-Min;Kim, Sang-Dal
    • Microbiology and Biotechnology Letters
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    • v.36 no.4
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    • pp.326-335
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    • 2008
  • The siderophore ($siderophore_{AH18}$) of Bacillus subtilis AR18 was determined to be one of catechol type and purified by using Amberlite XAD-2, Sephadex LR-20 chromatography, and reversed-phase RPLC. The $Siderophore_{AH18}$ was identified bacillibactin with its structure by GC-MS, $^1H$-NMR, and $^{13}C$-NMR. $Siderophore_{AH18}$ (bacillibactin) had been confirmed its molecular weight of 883 and chemical structure of $(2,3-dihydroxybenzoate-glycine-threonine)_3$. Purified $siderophore_{AH18}$ showed strong biocontrol ability towards the spore of Phytophthora capsici on PDA and able to effectively suppress (55%) P. capsici causing red-pepper blight in the pot in vivo test.

Assessing metabolic properties of dairy cows fed low quality straws by integrative arterial and venous metabolomics

  • Wang, Bing;Yu, Zhu;Liu, Jianxin
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.11
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    • pp.1770-1778
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    • 2020
  • Objective: This study was conducted to reveal potential metabolic differences of dairy cows fed corn stover (CS) and rice straw (RS) instead of alfalfa hay (AH) as main forage source. Methods: Thirty multiparous mid-late lactation Holstein dairy cows were selected and randomly assigned to three diets, AH, CS, or RS (n = 10). After 13 weeks of the feeding trial, coccygeal arterial and superficial epigastric venous plasma samples were collected before morning feeding for gas chromatography time-of-flight/mass spectrometry analyses. Results: In the artery, 8 and 13 metabolites were detected as differential metabolites between AH and CS, and between AH and RS, respectively. The relative abundance of phenylpropanoate (log2fold change [FC]) = 1.30, 1.09), panthenol (log2FC = 2.36, 2.20), threitol (log2FC = 1.00, 1.07), and 3,7,12-trihydroxycoprostane (log2FC = 0.79, 0.78) were greater in both CS and RS than in AH, and tyrosine (log2FC = -0.32), phenylalanine (log2FC = -0.30), and pyruvic acid (log2FC = -0.30) were lower in RS than in AH. In the vein, 1 and 7 metabolites were detected as differential metabolites between AH and CS, and between AH and RS, respectively. By comparing AH and RS, we found that metabolic pathways of phenylalanine, tyrosine, and tryptophan biosynthesis and phenylalanine metabolism were enriched by integrative artery and vein analysis. Furthermore, AH and RS, arterial phenylpropanoate and 4-hydroxyproline were positively, and phenylalanine was negatively correlated with milk urea nitrogen. Finally, in AH and CS, arterial panthenol was negatively correlated with feed efficiency. Conclusion: Arterial metabolic profiles changed more than those in the veins from animals on three forage diets, differing in amino acids. We found that phenylalanine, tyrosine, and tryptophan biosynthesis and phenylalanine metabolism were restricted when cows were fed low-quality cereal straw diets.

Microscopic Identification of the Chinese Patent Medicine 'Ah Myeong Dan' (아명단(兒明丹)의 현미감정연구)

  • Kim, Jeong-Myo;Park, Jong-Hee
    • Korean Journal of Pharmacognosy
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    • v.36 no.3 s.142
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    • pp.205-208
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    • 2005
  • Ah Myeong Dan (兒明丹) is Chinese patent medicine which is used for congenital fever and congenital boils in Korea. It consists of 11 kinds of powdered crude drugs. In powdered crude drugs, it is hard to identify each component by chemical analysis or morphological examination. However, the method of identification of powdered crude drugs has not been clearly established. Therefore, it is of interest to establish the microscopic method for identification of powdered crude drugs of Chinese patent medicines. The effectiveness of microscopic method is exemplified by the identification of tissue and contents of crude drugs by comparison with standard drugs. In this study, it is demonstrated that the microscopic method is very effective for the identification of 11 crude drug ingredients in Ah Myeong Dan.

Study on B-H Curve Characteristic of Magnetic Substance According to Tensile Stress (인장 응력에 따른 자성체의 B-H Curve 특성 연구)

  • Kim, Jong-Wang;Kim, Sang-Hyun;Yang, Hoon-Suk;Kim, Ji-Ho;Jung, Hyun-Ju;Lee, Hyang-Beom
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.64 no.3
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    • pp.417-421
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    • 2015
  • In this paper, study for B-H curve characteristics of the magnetic material due to tensile stresses of submarine for magnetic silence. For the experiment, made the sample of the material of AH36 and made to a B-H curve Tracer. In addition, design and made a system of stress by the weight in order to apply a tensile stress, it was possible to obtain results for the change of the magnetization.