• 제목/요약/키워드: AH36

검색결과 472건 처리시간 0.041초

Suppression of Migration and Invasion by Alnus hirsuta in Human Hepatocellular Carcinoma Cells

  • Bo-Ram Kim;Su Hui Seong;Tae-Su Kim;Jin-Ho Kim;Chan Seo;Ha-Nul Lee;Sua Im;Jung Eun Kim;Ji Min Jung;Jung Up Park;Kyung-Min Choi;Jin-Woo Jeong
    • 한국자원식물학회지
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    • 제36권3호
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    • pp.207-218
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    • 2023
  • Hepatocellular carcinoma (HCC) has a poor prognosis and high metastasis and recurrence rates. Although extracts of Alnus hirsuta (Turcz. ex Spach) Rupr. (AH) have been demonstrated to possess potential anti-inflammatory and anti-cancer activities, the underlying mechanism of AH in HCC treatment remains to be elucidated. We investigated the effects and potential mechanisms of AH on migration and invasion of Hep3B cells. Within the non-cytotoxic concentration range, AH significantly inhibited motility and invasiveness of Hep3B cells in a concentration-dependent manner. Inhibitory effects of AH on cell invasiveness are associated with tightening of tight junctions (TJs), as demonstrated by an increase in transepithelial electrical resistance. Immunoblotting indicated that AH decreased levels of claudins, which form major components of TJs and play key roles in the control and selectivity of paracellular transport. Furthermore, AH inhibited the expression and activity of matrix metalloproteinase (MMP)-2 and MMP-9 and simultaneously increased the levels of tissue inhibitor of metalloproteinase (TIMP)-1 and TIMP-2. These effects were related to inactivation of the phosphoinositide 3-kinase (PI3K)/AKT pathway in Hep3B cells. Therefore, AH inhibits migration and invasion of Hep3B cells by inhibiting the activity of MMPs and tightening TJs through suppression of claudin expression, possibly by suppressing the PI3K/AKT signaling pathway.

조선용 강판 AH36의 레이저-아크 하이브리드 용접시 맞대기 용접 특성 (Butt Weldability of Shipbuilding Steel AH36 Using Laser-Arc Hybrid Welding)

  • 김종도;명기훈;서정
    • 대한기계학회논문집A
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    • 제40권10호
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    • pp.901-906
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    • 2016
  • 본 논문은 두께 10 mm의 조선용 강판 AH36에 대해 레이저-아크 하이브리드 용접을 사용하여 원패스 관통용접을 실시함으로써 실제 조선 산업에서의 생산성 향상을 목적으로 연구되었다. 10 mm두께의 후판을 사용하였기 때문에 관통용접을 얻기 위해서는 더욱 높은 레이저 및 아크의 출력이 요구되었다. 그러나 보다 더 증가된 레이저 및 아크 출력의 사용은 비드 양단에 언더컷과 같은 결함을 야기하였다. 이러한 언더컷은 노치로 작용하여 용접 구조물의 강도를 약화시키므로 반드시 방지되어야하며, 본 실험에서는 아크의 변수인 용접 전압 및 펄스컬렉션을 조절함으로써 제어할 수 있었다. 용접된 시험편의 기계적 특성을 파악하기 위해 레이저 영역 및 아크 영역에 대해 경도 측정을 실시하였다. 그 결과, 열영향부는 조직의 변태로 인해 최대 경도가 용접부보다 높은 것을 확인할 수 있었다. 미세조직 관찰 결과, 열영향부는 마르텐사이트 및 베이나이트와 같은 경한 조직으로 구성되어 있는 것을 알 수 있었다.

AH36-TMCP강의 용접후열처리 효과에 관한 연구 (A Study on Effect of PWHT in AH36-TMCP Steel)

  • 유효선;장원상;안병국;정세희
    • Journal of Welding and Joining
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    • 제16권6호
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    • pp.44-51
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    • 1998
  • It is well known that the fine bainitic microstructure obtained by TMCP(thermo-mechanical control process) secures the high toughness of base metal. Besides, TMCP steel is very suitable for high heat input in welding as it has low carbon equivalent. In HAZ, however, the accelerated cooling effect imparted on the matrix by the weld thermal cycles is relieved and thus the weldment of TMCP steel has softening zone which shows low fracture toughness compared with base metal. Therefore, PHWT of weldment is carried out to improve the fracture toughness in weldment of TMCP steel which has softening zone. In this study, the effects of PWHT on the weldment of AH36-TMCP steel are investigated by the small punch (SP) test. From the several results such as SP energy and displacement at room temperature, the behavior of transition curves, the fracture strength at -196$^{\circ}C$, distribution of (DBTT)sp and (DBTT)sp, the PWHT condition of A.C. after 85$0^{\circ}C$-1 sec W.C. was suitable condition for recovering a softening zone of HAZ as welded.

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Bacillus subtilis AH18의 고추역병 방제능과 $Siderophore_{AH18}$의 구조분석 (Structural Identification of $Siderophore_{AH18}$ from Bacillus subtilis AH18, a Biocontrol agent of Phytophthora Blight Disease in Red-pepper)

  • 우상민;김상달
    • 한국미생물·생명공학회지
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    • 제36권4호
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    • pp.326-335
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    • 2008
  • Bacillus subtilis AH18균주는 auxin, siderophore 그리고 cellulase를 동시에 생산하는 PGPR 균주이자 생물방제균주로 항진균성 siderophore의 특성을 확인한 결과 catechol type의 siderophore로 확인하였다. $Siderophore_{AH18}$ 의 정제는 Amberlite XAD-2, sephadex LH-20 column chromatography 그리고 HPLC를 통해 정제 및 정제여부를 확인하였으며, GC-MS, $^1H$-NMR, 그리고 $^{13}C$-NMR을 통해 구조 및 분자량을 확인하였다. 그 결과 B. subtilis AH18이 생산하는 siderophore은 분자량 883의 bacillibactin임을 확인하였으며, 포자발아억제활성을 나타냄을 확인하였다. B. subtilis AH18은 P. capsici에 의한 고추역병을 효과적으로 방제하였으며(방제력 55%), 이는 bacillibactin에 의한 효과가 포함되리라 추측된다.

Assessing metabolic properties of dairy cows fed low quality straws by integrative arterial and venous metabolomics

  • Wang, Bing;Yu, Zhu;Liu, Jianxin
    • Asian-Australasian Journal of Animal Sciences
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    • 제33권11호
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    • pp.1770-1778
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    • 2020
  • Objective: This study was conducted to reveal potential metabolic differences of dairy cows fed corn stover (CS) and rice straw (RS) instead of alfalfa hay (AH) as main forage source. Methods: Thirty multiparous mid-late lactation Holstein dairy cows were selected and randomly assigned to three diets, AH, CS, or RS (n = 10). After 13 weeks of the feeding trial, coccygeal arterial and superficial epigastric venous plasma samples were collected before morning feeding for gas chromatography time-of-flight/mass spectrometry analyses. Results: In the artery, 8 and 13 metabolites were detected as differential metabolites between AH and CS, and between AH and RS, respectively. The relative abundance of phenylpropanoate (log2fold change [FC]) = 1.30, 1.09), panthenol (log2FC = 2.36, 2.20), threitol (log2FC = 1.00, 1.07), and 3,7,12-trihydroxycoprostane (log2FC = 0.79, 0.78) were greater in both CS and RS than in AH, and tyrosine (log2FC = -0.32), phenylalanine (log2FC = -0.30), and pyruvic acid (log2FC = -0.30) were lower in RS than in AH. In the vein, 1 and 7 metabolites were detected as differential metabolites between AH and CS, and between AH and RS, respectively. By comparing AH and RS, we found that metabolic pathways of phenylalanine, tyrosine, and tryptophan biosynthesis and phenylalanine metabolism were enriched by integrative artery and vein analysis. Furthermore, AH and RS, arterial phenylpropanoate and 4-hydroxyproline were positively, and phenylalanine was negatively correlated with milk urea nitrogen. Finally, in AH and CS, arterial panthenol was negatively correlated with feed efficiency. Conclusion: Arterial metabolic profiles changed more than those in the veins from animals on three forage diets, differing in amino acids. We found that phenylalanine, tyrosine, and tryptophan biosynthesis and phenylalanine metabolism were restricted when cows were fed low-quality cereal straw diets.

아명단(兒明丹)의 현미감정연구 (Microscopic Identification of the Chinese Patent Medicine 'Ah Myeong Dan')

  • 김정묘;박종희
    • 생약학회지
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    • 제36권3호통권142호
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    • pp.205-208
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    • 2005
  • Ah Myeong Dan (兒明丹) is Chinese patent medicine which is used for congenital fever and congenital boils in Korea. It consists of 11 kinds of powdered crude drugs. In powdered crude drugs, it is hard to identify each component by chemical analysis or morphological examination. However, the method of identification of powdered crude drugs has not been clearly established. Therefore, it is of interest to establish the microscopic method for identification of powdered crude drugs of Chinese patent medicines. The effectiveness of microscopic method is exemplified by the identification of tissue and contents of crude drugs by comparison with standard drugs. In this study, it is demonstrated that the microscopic method is very effective for the identification of 11 crude drug ingredients in Ah Myeong Dan.

인장 응력에 따른 자성체의 B-H Curve 특성 연구 (Study on B-H Curve Characteristic of Magnetic Substance According to Tensile Stress)

  • 김종왕;김상현;양훈석;김지호;정현주;이향범
    • 전기학회논문지
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    • 제64권3호
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    • pp.417-421
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    • 2015
  • In this paper, study for B-H curve characteristics of the magnetic material due to tensile stresses of submarine for magnetic silence. For the experiment, made the sample of the material of AH36 and made to a B-H curve Tracer. In addition, design and made a system of stress by the weight in order to apply a tensile stress, it was possible to obtain results for the change of the magnetization.