• BMB Reports
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• v.43 no.5
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• pp.375-381
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• 2010

In this study, the cDNA library of Chang-liver cells was immunoscreened using common ADAMs antibody to obtain ADAM related genes. We found one positive clone that was confirmed as a new gene by Blast, which is an uncharacterized helical and coil protein and processes protease activity, and named protease-related protein 1 (ARP1). The submitted GenBank accession number is AY078070. Molecular characterizations of ARP1 were analyzed with appropriate bioinformatics software. To analyse its expression and function, ARP1 was subcloned into glutathione S-transferase fusion plasmid pGEX-2T and expressed by E. coli system. The in vitro expression product of ARP1 was recognized by common ADAMs antibody with western blot. Interestingly, ARP1 cleaves gelatine at pH9.5, which suggests it is an alkaline protease. Semi-quantitative RT-PCR result indicates that ARP1 mRNA is strongly transcribed in the liver and the treated Chang-liver cells.

• Bulletin of the Korean Mathematical Society
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• v.55 no.6
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• pp.1859-1868
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• 2018

In the present paper, we have proved the sharp inequality ${\mid}H_{3,1}(f){\mid}{\leq}4$ and ${\mid}H_{3,1}(f){\mid}{\leq}1$ for analytic functions f with $a_n:=f^{(n)}(0)/n!$, $n{\in}{\mathbb{N}},$, such that $$Re\frac{f(z)}{z}>{\alpha},\;z{\in}{\mathbb{D}}:=\{z{\in}{\mathbb{C}}:{\mid}z{\mid}<1\}$$ for ${\alpha}=0$ and ${\alpha}=1/2$, respectively, where $$H_{3,1}(f):=\left|{\array{{\alpha}_1&{\alpha}_2&{\alpha}_3\\{\alpha}_2&{\alpha}_3&{\alpha}_4\\{\alpha}_3&{\alpha}_4&{\alpha}_5}}\right|$$ is the third Hankel determinant.

• Computers and Concrete
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• v.14 no.2
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• pp.145-161
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• 2014

The article presents the statistical method of grouping the results of the compressive strength of concrete in continuous production. It describes the method of dividing the series of compressive strength results into batches of statistically stable strength parameters at specific time intervals, based on the standardized concept of "concrete family". The article presents the examples of calculations made for two series of concrete strength results, from which sets of decreased strength parameters were separated. When assessing the quality of concrete elements and concrete road surfaces, the principal issue is the control of the compressive strength parameters of concrete. Large quantities of concrete mix manufactured in a continuous way should be subject to continuous control. Standardized approach to assessing the concrete strength proves to be insufficient because it does not allow for the detection of subsets of the decreased strength results, which in turn makes it impossible to make adjustments to the concrete manufacturing process and to identify particular product or area on site with decreased concrete strength. In this article two independent methods of grouping the test results of concrete with statistically stable strength parameters were proposed, involving verification of statistical hypothesis based on statistical tests: Student's t-test and Mann - Whitney - U test.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.37 no.1
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• pp.99-104
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• 2009

In this paper, the drop characteristic of the small aircraft landing gear of SUAV has been analyzed and performed on orifice optimal design for shock absorption efficiency. The SUAV landing gear was simple oleo pneumatic type without metering pin. The landing gear was modelled by MSC ADAMS software. Drop test evaluation was conducted to confirm the analysis model. As a result of correlation between analysis and test results, it was verified that these results were coincided with very well. After confidence review of analysis model through the correlation between test and analysis results, design parametric study was performed by using confirmed analysis model. Optimal orifice size with best efficiency have been decided in this study.

• Journal of Multimedia Information System
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• v.3 no.3
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• pp.97-102
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• 2016

Deep learning enables machines to have perception and can potentially outperform humans in the medical field. It can save a lot of time and reduce human error by detecting certain patterns from medical images without being trained. The main goal of this paper is to build the optimal model for breast mass classification by applying various methods that influence the performance of Convolutional Neural Network (CNN). Google's newly developed software library Tensorflow was used to build CNN and the mammogram dataset used in this study was obtained from 340 breast cancer cases. The best classification performance we achieved was an accuracy of 0.887, sensitivity of 0.903, and specificity of 0.869 for normal tissue versus malignant mass classification with augmented data, more convolutional filters, and ADAM optimizer. A limitation of this method, however, was that it only considered malignant masses which are relatively easier to classify than benign masses. Therefore, further studies are required in order to properly classify any given data for medical uses.

• Journal of Microbiology and Biotechnology
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• v.20 no.9
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• pp.1307-1313
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• 2010

An extracellular ${\alpha}$-amylase from Lactococcus lactis IBB500 was purified and characterized. The optimum conditions for the enzyme activity were a pH of 4.5, temperature of $35^{\circ}C$, and enzyme molecular mass of 121 kDa. The genome analysis and a plasmid curing experiment indicated that $amy^+$ genes were located in a plasmid of 30 kb. An analysis of the phylogenetic relationships strongly supported a hypothesis of horizontal gene transfer. A strong homology was found for the peptides with the sequence of ${\alpha}$-amylases from Ralstonia pikettii and Ralstonia solanacearum. The protein with ${\alpha}$-amylase activity purified in this study is the first one described for the Lactococcus lactis species, and this paper is the first report on a Lactococcus lactis strain belonging to the amylolytic lactic acid bacteria (ALAB).

• Journal of the Optical Society of Korea
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• v.18 no.6
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• pp.691-697
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• 2014

Optical measurements of the morphological and biochemical imaging of phytoplankton are presented. Employing quantitative phase imaging techniques, 3-D refractive index maps and high-resolution 2-D quantitative phase images of individual live phytoplankton are simultaneously obtained without exogenous labeling agents. In addition, biochemical information of individual phytoplankton including volume, mass, and density of individual phytoplankton are also quantitatively obtained from the measured refractive index distributions. We expect the present method to become a powerful tool for the study of phytoplankton.

• Clinical and Experimental Reproductive Medicine
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• v.41 no.3
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• pp.120-124
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• 2014

Objective: The aim of the present study was to examine whether interactions between polymorphisms in the thyroglobulin and ADAM metallopeptidase with thrombospondin type 1 motif, 16 (ADAMTS16) genes are associated with the development of premature ovarian failure (POF). Methods: A total of 75 patients with POF and 196 controls were involved in this study. We used a GoldenGate assay to genotype single nucleotide polymorphisms (SNPs). Logistic regression analysis was performed to identify POF-associated polymorphisms and synergistic interactions between polymorphisms in the thyroglobulin and ADAMTS16 genes. Results: Single gene analyses using logistic regression analysis showed no significant association between polymorphisms in the two genes and POF. In the results from interaction analyses, we found seven synergistic interactions between the polymorphisms in thyroglobulin and ADAMTS16, although there was no combination showing p-values lower than the significant threshold using the Bonferroni correction. When the AG genotype was present at the rs853326 missense SNP, the A and G alleles at the tagging SNPs rs16875268 and rs13168665 showed significant interactions (odds ratios=5.318 and 16.2 respectively; 95% confidence intervals, 1.64-17.28 and 2.08-126.4; p=0.0054 and 0.0079). Conclusion: Synergistic interactions between polymorphisms in the thyroglobulin and ADAMTS16 genes were associated with an increased risk of POF development in Korean women.

• Composites Research
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• v.25 no.6
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• pp.230-235
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• 2012

This paper presents the vibration characteristics and the optimization using the orthogonal array about applied composite optical structure of an aircraft. To acquire the vibration characteristics for stable line of sight, modal analysis are performed by using multi-body program ADAMS. And to optimize optical structure, for design variables were selected, larger-the-better characteristics were considered using results of S/N ratio and orthogonal array $L_9(3^4)$. When bearing constraints are selected, radial, axial and moment stiffness value are used to analysis for optimization until now. But B.S.R which is non-dimensional parameter is proposed, structures including bearings can be used for optimization. And then having a result of lager-the-better, the optimized values of each design variable were successfully suggested.

• Korean Journal of Plant Resources
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• v.30 no.6
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• pp.601-611
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• 2017

Hyperlipidemia is an increase in one or more of the plasma lipids, including triglycerides, cholesterol. Ginseng has been used as a valuable tonic and for the treatment of various diseases. The objective of this study was to evaluate the effects of Jinan red ginseng (JRG) water extract on the blood and serum in rabbits with hyperlipidemia induced by poloxamer 407 when supplied in drinking water. JRG treatment was performed for 20 weeks. We evaluated the effects of the JRG treatment on diabetes through hematological and biochemical analysis before and after JRG treatment were performed. Our results indicate that LDL, total cholesterol, and triglycerides were significantly decreased compared prior JRG supply. CRE, BUN, CK and UA levels indicating renal functions are significantly reduced when compared to those prior to the JRG supply. In addition, AST, ALT, ALP, and LDH were significantly reduced indicating hepatoprotective effect. Blood electrolytes deteriorated in HL rabbits were improved when JRG supplied. In conclusion, Biochemical and hematological analysis demonstrate that the JRG is effective to alleviate the hyperlipidemia signs.