Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Effects of the Jinan Red Ginseng Extract Treatment on Poloxamer 407-induced Hyperlipidemia in Rabbits

  • Choi, Kyung-Min (Nakdonggang Institute of Biological Resources) ;
  • Lee, Jeong Ho (Sunchang Research Institute of Health and Longevity) ;
  • Adam, Gareeballah Osman (Department of Pharmacology, College of Veterinary Medicine, Chonbuk National University, Iksan Campus) ;
  • Kim, Shang-Jin (Department of Pharmacology, College of Veterinary Medicine, Chonbuk National University, Iksan Campus) ;
  • Kang, Hyung-Sub (Department of Pharmacology, College of Veterinary Medicine, Chonbuk National University, Iksan Campus) ;
  • Yang, Yeong-Seok (Division of Pharmaceutical Engineering, Woosuk University) ;
  • Kim, Gi-Beum (Korea Pickle Co., LTD.)
  • Received : 2017.08.09
  • Accepted : 2017.10.16
  • Published : 2017.12.31
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Abstract

Hyperlipidemia is an increase in one or more of the plasma lipids, including triglycerides, cholesterol. Ginseng has been used as a valuable tonic and for the treatment of various diseases. The objective of this study was to evaluate the effects of Jinan red ginseng (JRG) water extract on the blood and serum in rabbits with hyperlipidemia induced by poloxamer 407 when supplied in drinking water. JRG treatment was performed for 20 weeks. We evaluated the effects of the JRG treatment on diabetes through hematological and biochemical analysis before and after JRG treatment were performed. Our results indicate that LDL, total cholesterol, and triglycerides were significantly decreased compared prior JRG supply. CRE, BUN, CK and UA levels indicating renal functions are significantly reduced when compared to those prior to the JRG supply. In addition, AST, ALT, ALP, and LDH were significantly reduced indicating hepatoprotective effect. Blood electrolytes deteriorated in HL rabbits were improved when JRG supplied. In conclusion, Biochemical and hematological analysis demonstrate that the JRG is effective to alleviate the hyperlipidemia signs.

Keywords

References

  1. Bagdade, J., A. Casarettoand and J. Albers. 1976. Effects of chronic uremia, hemodialysis, and renal transplantation on plasma lipids and lipoproteins in man. J. Lab. Clin. Med. 87:38-48.
  2. Baranov, A.I. 1982. Medicinal uses of ginseng and related plants in the Soviet Union: Recent trends in the soviet literature. J. Ethnopharmacol. 6:339-353. https://doi.org/10.1016/0378-8741(82)90055-1
  3. Burtis, C.A. and E.R. Ashwood. 1994. Textbook of Clinical Chemistry. 2nd ed., Saunders Company, Philadelphia, USA. pp. 324-1327.
  4. Chan, D.T., G.K. Dogra, A.B. Irish, E.M. Ooi, P.H. Barrett, D.C. Chan and G.F. Watts. 2009. Chronic kidney disease delays VLDL apoB-100 particle catabolism: potential role of apo C-III. J. Lipid Res. 50:2524-2531. https://doi.org/10.1194/jlr.P900003-JLR200
  5. Chen, I.J., M.Y. Chang, S.L. Chiao, J.L. Chen, C.C. Yu, S.H. Yang, J.M. Liu, C.C. Hung. R.C. Yang, H.C. Chang, H.C. Hsu and J.T. Fang. 2012. KRG Improves Blood Pressure Stability in Patients with Intradialytic Hypotension. eCAM. 2012:595271.
  6. Cheung, A.K., C.J. Parker, K. Ren and P.H. Iverius. 1996. Increased lipase inhibition in uremia: identification of pre-beta-HDL as a major inhibitor in normal and uremic plasma. Kidney Int. 49:1360-1371. https://doi.org/10.1038/ki.1996.192
  7. Chobanian, A.V. 1991. Single risk factor intervention may be inadequate to inhibit atherosclerosis progression when hypertension and hypercholesterolemia coexist. Hypertension 18:130-131. https://doi.org/10.1161/01.HYP.18.2.130
  8. Choi, K.T. 2008. 2008. Botanical characteristics, pharmacological effects and medicinal components of Korean Panax ginseng C. A. Meyer. Acta. Pharmacol. Sin. 29:1109-1118. https://doi.org/10.1111/j.1745-7254.2008.00869.x
  9. Corica, F., A. Corsonello, R. Ientile, D. Cucinotta, A. Di Benedtto, F, Perticone, L.J. Dominguez and M. Barbagallo. 2006. Serum ionized magnesium levels in relation to metabolic syndrome in type 2 diabetic patients. J. Am. Coll. Nutr. 25:210-215. https://doi.org/10.1080/07315724.2006.10719534
  10. Desai, S.D. and J. Blanchard. 1998. In vitro evaluation of Pluronic F127-based controlled-release ocular delivery systems for pilocarpine. J. Pharm. Sci. 87:226-230. https://doi.org/10.1021/js970090e
  11. Dimeski, G., P. Mollee and A. Carter. 2006. Effects of hyperlipidemia on plasma sodium, potassium, and chloride measurements by an indirect ion-selective electrode measuring system. Clin. Chem. 52(1):155-156. https://doi.org/10.1373/clinchem.2005.054981
  12. Ernst, E. 2010. Panax ginseng: an overview of the clinical evidence. JGR. 34:259-263.
  13. Farias, R.A., M.F. Neto, G.S. Viana and V.S. Rao. 1996. Effects of Croton cajucara extract on serum lipids of rats fed a high fat diet. Phytother. Res. 10:697-699. https://doi.org/10.1002/(SICI)1099-1573(199612)10:8<697::AID-PTR925>3.0.CO;2-I
  14. Gong, W.H., W.X. Zheng, J. Wang, S.H. Chen, B. Pangand and X.M. Hu, 2012. Coexistence of hyperlipidemia and acute cerebral ischemia/reperfusion induces severe liver damage in a rat model. World J. Gastroenterol. 18(35):4934-4943. https://doi.org/10.3748/wjg.v18.i35.4934
  15. Guerrero-Romero, F. and M. Rodriguez-Moran. 2000. Hypomagnesemia is linked to low serum HDL-cholesterol irrespective of serum glucose values. J. Diabetes Complications 14:272-276. https://doi.org/10.1016/S1056-8727(00)00127-6
  16. Guerrero-Romero, F. and M. Rodriguez-Moran. 2002. Low serum magnesium levels and metabolic syndrome. Acta. Diabetol. 39:209-213. https://doi.org/10.1007/s005920200036
  17. Hirano, T., T. Sakaue, A. Misaki, S. Murayama, T. Takahashi, K. Okada, H. Takeuchi, G. Yoshino and M. Adachi. 2003. Very low-density lipoproteinapoprotein CI is increased in diabetic nephropathy: comparison with apoprotein CIII. Kidney Int. 63:2171-2177. https://doi.org/10.1046/j.1523-1755.2003.00019.x
  18. Institute of Laboratory Animal Resources; Commission on Life Sciences; National Research Council. 1996. Guide for the Care and Use of Laboratory Animals. National Academy Press: Washington, DC (USA). pp. 1-124.
  19. Jeyabalan, S. and M. Palayan. 2009. Antihyperlipidemic activity of Sapindus emarginatus in triton WR-1339 induced albino rats. Res. J. Pharm. Tech. 2(2):319-323.
  20. Joan, M.B., B. Linda, C.F. Jon and J.R. Gary. 1999. Dose-dependent hyperlipidemia in rabbits following administration of poloxamer 407 gel. Pharmacology Letters Accelerated Communication. Life Sci. 65:261-266.
  21. Johnston, T.P. 2004. The P-407-induced murine model of dose-controlled hyperlipidemia and atherosclerosis: a review of findings to date. J. Cardiovasc. Pharmacol. 43:595-606. https://doi.org/10.1097/00005344-200404000-00016
  22. Johnston, T.P. and W.K. Palmer. 1993. Mechanism of poloxamer 407-induced hypertriglyceridemia in the rat. Biochem. Pharmacol. 46:1037-1042. https://doi.org/10.1016/0006-2952(93)90668-M
  23. Kim, Y.J., M.Y. Lee, Y.H. Son, B.K. Park, S.Y. Ryu and J.Y. Jung. 2014. Red ginseng ameliorates acute cisplatin-induced nephropathy. Planta. Med. 80:645-654. https://doi.org/10.1055/s-0034-1368571
  24. Klionsky, D.J., H. Abeliovich, P. Agostinis, D.K. Agrawal, G. Aliev and D.S. Askew, et al. 2008. Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes. Autophagy 4(2):151-175. https://doi.org/10.4161/auto.5338
  25. Larkin, B.G. and R.J. Zimmanck. 2015. Interpreting Arterial Blood Gases Successfully. AORN Journal 102:344-359
  26. Lee, J.H. and C.S. Jeong. 2008. Inhibitory effects of ginsenoside Rb1, Rg3, and Panax ginseng head butanol fraction on inflammatory mediators from LPS-stimulated RAW 264.7 cells. Biomol. Therapeut. 16:277-285. https://doi.org/10.4062/biomolther.2008.16.3.277
  27. Lewington, S., G. Whitlock, R. Clarke, P. Sherliker, J. Emberson, J. Halsey, N. Qizilbash, R. Peto and R. Collins. 2007. Blood cholesterol and vascular mortality by age, sex, and blood pressure: a meta-analysis of individual data from 61 prospective studies with 55,000 vascular deaths. Lancet 370:1829-1839 https://doi.org/10.1016/S0140-6736(07)61778-4
  28. Michael, W.P. and K. Dermot. 1997. The effect of solubility and hyperlipidemia on perioperative arterial blood gas tensions. JCA. 9:331-333.
  29. Min, S.W., S.H. Jung, K.H. Cho and D.H. Kim. 2008. Antihyperlipidemic effects of red ginseng, crataegii fructus and their main constituents ginsenoside Rg3 and ursolic acid in mice. Biomol. Therapeut. 16:364-369. https://doi.org/10.4062/biomolther.2008.16.4.364
  30. Mishra, P.R., P.K. Panda, K.C. Apanna and S. Panigrahi. 2011. Evaluation of acute hypolipidemic activity of different plant extracts in Triton WR-1339 induced in albino rats. Pharmacology online. 3:925-934.
  31. Moberly, J.B., P.O. Attman, O. Samuelsson, A.C. Johansson, C. Knight- Gibson and P. Alaupovic. 1999. Apolipoprotein C-III, hypertriglyceridemia and triglyceride-rich lipoproteins in uremia. Miner. Electrolyte Metab. 25:258-262. https://doi.org/10.1159/000057457
  32. Nabavi, S.F., A. Sureda, S. Habtemariam and S.M. Nabavi. 2015. Ginsenoside Rd and ischemic stroke; a short review of literatures. J. Ginseng Res. 39:299-303. https://doi.org/10.1016/j.jgr.2015.02.002
  33. Namama, T. 2015. Serum electrolytes and lipid profiles in non-insulin dependent diabetes mellitus patients. AJMS. 6:38-41.
  34. Nerbrand, C., L. Agreus, R. A. Lenner, P. Nyberg and K. Svardsudd. 2003. The influence of calcium and magnesium in drinking water and diet on Cardio-vascular risk factors in individuals living in hard and soft water areas with differences in cardiovascular mortality. BMC Public Health 3:21-29. https://doi.org/10.1186/1471-2458-3-21
  35. Paolisso, G., S. Sgambato, G. Pezza, N. Passariello, M. Varricchio and F. D'Onofrio. 1989. Improved insulin response and action by chronic magnesium administration in aged NIDDM subjects. Diabetes Care 12:265-269. https://doi.org/10.2337/diacare.12.4.265
  36. Park, T.Y. and J.Y. Cho. 2008. Inhibitory Effect of Ginsenoside-Rp1, a Novel Ginsenoside Derivative, on the Functional Activation of Macrophage-like Cells. Biomol. Therapeut. 16:370-376. https://doi.org/10.4062/biomolther.2008.16.4.370
  37. Park, J.D., D.K. Rhee and Y.H. Lee. 2005. Biological activities and chemistry of saponins from Panax ginseng C. A. Meyer. Photochemistry Reviews 4:159-175. https://doi.org/10.1007/s11101-005-2835-8
  38. Park, K.H., S.J. Kim, J.Y. Kim, K.J. Min, J.R. Kim and K.H. Cho. 2017. Cold-water extract of KRG exhibits potent inhibitory effects against cholesteryl ester transfer protein activity and fructose-mediated glycation along with lipid-lowering activity in hyperlipidemic zebrafish. J. Ginseng Res. 1-12.
  39. Park, T.Y., M.H. Park, W.C. Shin, M.H. Rhee, D.W. Seo, J.Y. Cho and H.M. Kim. 2008. Anti-metastatic potential of ginsenoside Rp1, a novel ginsenoside derivative. Biol. Pharm. Bull. 31:1802-1805. https://doi.org/10.1248/bpb.31.1802
  40. Prinsen, B.H., M.G. de Sain-van der Velden, E.J. de Koning, H.A. Koomans, R. Berger and T.J. Rabelink. 2003. Hypertriglyceridemia in patients with chronic renal failure: possible mechanisms. Kidney Int. Suppl. 84:S121-S124.
  41. Qi, L.W., C.Z. Wang and C.S. Yuan. 2011. Isolation and analysis of ginseng: advances and challenges. Natural Product Reports 28:467-495. https://doi.org/10.1039/c0np00057d
  42. Rahman, M.M., S.J. Lee, A.R. Mun, G.O. Adam, R.M. Park, G.B. Kim, H.S. Kang, J.S. Kim, S.J. Kim and S.Z. Kim. 2014. Relationships between blood $Mg^{2+}$ and energy metabolites/enzymes after acute exhaustive swimming exercise in rats. Biol. Trace. Element. Res. 161:85-90. https://doi.org/10.1007/s12011-014-9983-x
  43. Rayssiguer, Y., L. Noe and J. Etienne. 1991. Effect of magnesium deficiency on post-heparin lipase activity and tissue lipoprotein lipase in the rats. Lipids 26:182-186. https://doi.org/10.1007/BF02543968
  44. Riggs, J. E. 2002. Neurologic manifestations of electrolyte disturbances. Neurol. Clin. 20:227-239. https://doi.org/10.1016/S0733-8619(03)00060-4
  45. Robles, N.R., J.M. Escola, L. Albarran and R. Espada. 1998. Correlation of serum magnesium and serum lipid levels in hemodialysis patients. Nephron 78:118-119. https://doi.org/10.1159/000044895
  46. Shimada, M., H. Shimano, T. Gotoda, K. Yamamoto, M. Kawamura, T. Inaba, Y. Yazaki and N. Yamada. 1993. Overexpression of human lipoprotein lipase in transgenic mice. J. Biul. Chem. 268:17924-17929.
  47. Sohn, S.H., S.K. Kim, Y.O. Kim, H.D. Kim, Y.S. Shin, S.O. Yang, S.Y. Kim and S.W. Lee. 2013. A comparison of antioxidant activity of Korean white and red ginseng on $H_2O_2$-induced oxidative stress in HepG2 hepatoma cells. J. Ginseng Res. 37:442-450. https://doi.org/10.5142/jgr.2013.37.442
  48. Sohn, E.H., T. Kim, Y.J. Jeong, H.S. Han, Y. Lea, Y.M. Cho and S.C. Kang. 2015. Triglyceride control effect of Agrimonia eupatoria L. in oleic acid induced NAFLD-HepG2 model. Korean J. Plant Res. 28(5):635-640. https://doi.org/10.7732/kjpr.2015.28.5.635
  49. Song, Y., T.Y. Li, R.M. Van Dam, J.E. Manson and F.B. Hu. 2007. Magnesium intake and plasma concentration of markers of systemic inflammation and endothelial dysfunction in women. Am. J. Clin. Nutr. 85:1068-1074. https://doi.org/10.1093/ajcn/85.4.1068
  50. Song, Y., P.M. Ridker, J.E. Manson, N.R. Cook and J.E. Buring. 2005. Liu. Magnesium intake, creative protein, and the prevalence of metabolic syndrome in middle-aged and older U.S. women. Diabetes Care 28:1438-1444. https://doi.org/10.2337/diacare.28.6.1438
  51. Spurzem, J.R., H.W. Bonekat and J.W. Shigeokaw. 1984. Factitious methemoglobinemia caused by hyperlipemia. Chest. 86:84-86. https://doi.org/10.1378/chest.86.1.84
  52. Stratton, L.P., A. Dong, M.C. Manning and J.F. Carpenter. 1999. Drug delivery matrix containing native protein precipitates suspended in a polomaxamer gel. J. Pharm. Sci. 86:1006-l009.
  53. Trent, C.M., S. Yu, Y. Hu, N. Skoller, L.A. Huggins, S. Homma and I.J. Goldberg. 2014. Lipoprotein lipase activity is required for cardiac lipid droplet production. J. Lipid Res. 55(4):645-658. https://doi.org/10.1194/jlr.M043471
  54. Tsimihodimos, V., Z. Mitrogianni and M. Elisaf. 2011. Dyslipidemia associated with chronic kidney disease. Open Cardiovasc. Med. J. 5:41-48. https://doi.org/10.2174/1874192401105010041
  55. Vaziri, N.D. and K. Liang. 1996. Down-regulation of tissue lipoprotein lipase expression in experimental chronic renal failure. Kidney Int. 50:1928-1935. https://doi.org/10.1038/ki.1996.515
  56. Vazquez-Perez, S., P. Aragoncillo, H.N. de Las, J. Navarro-Cid, E. Cediel, D. Sanz-Rosa, L.M. Ruilope, C. Diaz, G. Hernandez, V. Lahera and V. Cachofeiro. 2001. Atorvastatin prevents glomerulosclerosis and renal endothelial dysfunction in hypercholesterolaemic rabbits. Nephrol. Dial. Transplant. 16:40-41.
  57. Wasan, K.M., R. Subramanian, M. Kwong, I.J. Goldberg, T. Wright and T.P. Johnston. 2003. Poloxamer 407-mediated alterations in the activities of enzymes regulating lipid metabolism in rats. J. Pharm. Pharm. Sci. 6(2):189-197.
  58. Yokozawa, T., A. Ishida. E.J. Cho and T. Nakagawa. 2003. The effects of Coptidis Rhizoma extract on a hypercholesterolemic animal model. Phytomedicine. 10:17-22. https://doi.org/10.1078/094471103321648610
  59. Yun, T.K. 2001. Brief introduction of Panax ginseng C.A. Meyer. J. Korean Med. Sci. 16:S3-S5 (in Korean). https://doi.org/10.3346/jkms.2001.16.S.S3

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