• Korean Journal of Fisheries and Aquatic Sciences
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• v.47 no.4
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• pp.370-375
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• 2014

BACE2 is a membrane-bound aspartic protease that is highly homologous with BACE1. While BACE1 processes the amyloid precursor protein (APP) at a key step in generating ${\beta}$-amyloid peptide and presumably causes Alzheimer's disease (AD), BACE2 has not been demonstrated to be involved in APP processing directly, and its physiological functions are unknown. To determine its function and to develop inhibitors from marine sources, we constructed an overexpression vector for producing BACE2. The gene encoding human BACE2 protease was amplified using the polymerase chain reaction and cloned into the pET11a expression vector, resulting in pET11a/BACE2. Recombinant BACE2 protease was overexpressed successfully in E. coli as inclusion bodies, refolded using the rapid-dilution method, and purified via two-step fast protein liquid chromatography using Sephacryl S-300 gel filtration and Resource-Q column chromatography. The BACE2 protease produced was an active form. This study provides an efficient method not only for studying the basic properties of BACE2, but also for developing inhibitors from natural marine sources.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2006.05a
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• pp.417-420
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• 2006

The composition and structure of dendrite phase within $Zr_{76.11}Ti_{4.20}Cu_{4.51}Ni_{3.16}Be_{1.49}Nb_{10.53}$ bulk metallic glass (BMG) were confirmed by using an EPMA, XRD and TEM, respectively. The chief elements of dendrite phase were Zr-Ti-Nb and had a BCC structure. The thermal properties of this BMG have been then subsequently investigated by using a differential scanning calorimeter (DSC). The glass transition and crystallization onset temperatures were determined as $339.7^{\circ}C$ and $375.8^{\circ}C$ for this alloy, respectively. Mechanical properties have also been examined by conducting a series of uniaxial compression tests at various temperatures within supercooled liquid region under the strain rates between $10^{-4}/s$ and $3{\times}10^{-2}/s$. The deformation behavior of BMG composite within supercooled liquid region is similar to one of Vit-1 exhibiting amorphous single phase alloy. The flow stresses of BMG composite, however, are entirely higher than those of Vit-1 because dendrite phases are interfere with moving of atoms.

• YAKHAK HOEJI
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• v.52 no.5
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• pp.370-375
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• 2008

In this study, the anti-allergic activity and mechanim of Salviae radix (SR) were investigated. The ethanol extract of SR showed significant inhibitory effect on degranulation from antigen-stimulated mast cells and it also inhibited the expression and secretion of TNF-${\alpha}$ and IL-4 in antigen-stimulated RBL-2H3 cells. In the mast cell-mediated local animal allergy model, it suppressed the passive cutaneous anaphylaxis in a dose-dependent manner. As its mechanism of action, SR inhibited the activating phosphorylation of Syk, a downstream signaling molecule of Src-family kinase, for the activation of mast cells. The results of the study indicate that the anti-allergic activity of SR is mediated by the inhibition of Src-family kinase in mast cells.

• Journal of Integrative Natural Science
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• v.1 no.2
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• pp.79-83
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• 2008

As a new standard for performance measurement, NEMA NU2-2001 was presented recently. In this study, I investigated the spatial resolution, sensitivity, scatter fraction, and noise equivalent count ratio (NECR) in order to know the information of physical characteristics and system performance of GE discovery ST using this new standard. Bismuth germinate crystals ($6{\times}6$ array, $6.3mm{\times}6.3mm{\times}30mm$) were used in discovery ST (energy window:375-650 keV, coincidence window:11.7 nsec). To measure the sensitivity, five aluminum sleeves (Data Spectrum Corp., Chapel Hill, NC., USA, thickness:1.25 mm)-NEMA sensitivity phantom- filled with F-18 solution were used. Successive measurements in 2D and 3D acquisition mode were made with a line source at the center of transaxial field of view and 10 cm off from the center until the count was over 500,000. Spatial resolution was estimated using a point source (F-18, 0.1 mCi) at different locations in the FOV. Scatter fraction and NECR was tested using a NEMA scatter phantom. Dynamic data were acquired for 7 half-lives using F-18 solution. And true to background ratio was averaged at last three frames when the random rate was as small as ignorable for the calculation of scatter fraction. We anticipate this overall evaluated results could be used for the quality assurance and optimized image acquisition for clinical research.

• The Journal of Korean Academic Society of Nursing Education
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• v.17 no.2
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• pp.306-316
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• 2011

Purpose: The purpose of this study was to compare health promotion lifestyles of college nursing students between students who live with their parents and students who are self-boarders. Methods: The sample consisted of 375 college nursing students in C and S city. Self report questionnaires were used to measure the variables. In data analysis, the SPSSWIN 18.0 program was utilized for descriptive statistics, Pearson's correlation coefficients, and multiple regression analysis. Results: The mean score for Health Promotion Lifestyle in living with the parents group was significantly higher than that of the self-boarders group (t=-2.16, p=.031). In multiple regression, perceived health state, exercise frequency, self-rated knowledge about health, and grade were significant predictors of Health Promotion Lifestyle in college nursing students, explaining 26.3%. Conclusion: This study suggests that perceived health state, exercise frequency, and self-rated knowledge about health, and grade are significant influencing factors in Health Promotion Lifestyle in college nursing students. Therefore, strategies which improve these variables must be developed for college nursing students.

• Journal of Korean society of Dental Hygiene
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• v.23 no.6
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• pp.529-536
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• 2023

Objectives: This study aimed to determine the relationship between hours of smartphone use and oral disease symptoms in Korean adolescents. Methods: In this study, data from the 2022 Youth Health Behavior Online Survey were used. The final analysis was conducted on students who responded that they have used a smartphone (N=50,375). Chi-squared test and multivariate logistic regression analysis were used to analyze the relationship between oral disease symptoms and smartphone use time (p<0.05). Results: Consequent to examining the effect of smartphone use on oral disease symptoms, it was found that oral symptoms, such as pain during chewing and tingling throbbing, increased as smartphones were used for >4 h (p<0.05). Additionally, oral symptoms, such as gingivalgia bleeding and bad breath, appeared to increase as smartphones were used for >6 h (p<0.05). However, using smartphones for <0-2 h or 2-4 h had no effect on oral disease symptoms. Conclusions: In adolescents, oral disease symptoms increased significantly with increasing hours of smartphone use. Therefore, adolescents should be guided so that they use smartphones for <2 h or >4 h. Moreover, guidance on forming the habit of tooth brushing after eating food should be provided to prevent oral disease symptoms.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.21 no.2
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• pp.375-382
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• 1996

In this paper, aneffective method for computing source to terminal reliability of network by decomposition is described. A graph is modeled after a network, and decomposed into two subgraphs. A logic product term of one subgraph is computed, and a graph of the other subgraphs is made according to the event representing the logic product term, and it's logic product term is compted. By multiplying the logic product term of one subgraph by that of the other subgraphs, a method for computing the source to terminal reliability is proposed. the time complexity for computing all the logic product terms of one subgraph is the product of copies of the number of edges in the subgraph of 2, and that of the other subgraph is the number of edges multiplied by the number of logic product terms. This method requires less computation time than that not by decomposition.

• The Korean Journal of Nuclear Medicine Technology
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• v.17 no.2
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• pp.67-71
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• 2013

Purpose: The Change of CT exposure condition have a effect on image quality and patient exposure dose. In this study, we evaluated effect CT image quality and SUV when CT parameters (Pitch, Rotation time) were changed. Materials and Methods: Discovery Ste (GE, USA) was used as a PET/CT scanner. Using GE QA Phantom and AAPM CT Performance Phantom for evaluate Noise of CT image. Images are acquired by using 24 combinations that four stages pitch (0.562, 0.938, 1.375, 1.75:1) and six stages X-ray tube rotation time (0.5s-1.0s). PET images are acquired using 1994 NEMA PET Phantom ($^{18}F-FDG$ 5.3 kBq/mL, 2.5 min/frame). For noise test, noise are evaluated by standard deviation of each image's CT numbers. And then we used expectation noise according to change of DLP (Dose Length Product) to experimental noise ratio for index of effectiveness. For spatial resolution test, we confirmed that it is possible to identify to 1.0 mm size of the holes at the AAPM CT Performance Phantom. Finally we evaluated each 24 image's SUV. Results: Noise efficiency were 1.00, 1.03, 1.01, 0.96 and 1.00, 1.04, 1.02, 0.97 when pitch changes at the QA Phantom and AAPM Phantom. In case of X-ray tube rotation time changes, 0.99, 1.02, 1.00, 1.00, 0.99, 0.99 and 1.01, 1.01, 0.99, 1.01, 1.01, 1.01 at the QA Phantom and AAPM Phantom. We could identify 1.0 mm size of the holes all 24 images. Also, there were no significant change of SUV and all image's average SUV were 1.1. Conclusion: 1.75:1 pitch is the most effective value at the CT image evaluation according to pitch change and It doesn't affect to the spatial resolution and SUV. However, the change of rotation time doesn't affect anything. So, we recommend to use the effective pitch like 1.75:1 and adequate X-ray tube rotation time according to patient size.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.9
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• pp.375-386
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• 2016

This study was conducted to investigate the effectiveness of a nonviolent communication program on nurse's interpersonal competence, job stress and spiritual wellness. This study was a inequality similar to the control group experimental design study of repeated measurements before and after. The data for this study were collected from June 1 to October 20, 2013 from 29 clinical nurses. The self-administered questionnaires used consisted of questions regarding general characteristics, interpersonal competence, job stress and spiritual wellness. The results were: 1. For interpersonal competence, the interaction between time and group was significant (F = 7.726, p = 0.002). 2. For job stress analysis, the interaction between time and group was not significant (F = 0.142, p = 0.851). 3. For spiritual wellness, the interaction between time and group was significant (F = 31.355, p < .001). These results suggest that the nonviolent communication program had a positive effect on rejected clinical nurse's interpersonal competence and spiritual wellness, but not on job stress. A follow-up replication study will be necessary.

• Molecules and Cells
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• v.28 no.4
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• pp.375-382
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• 2009

The 26S proteasome is a 2-MDa complex with a central role in protein turn over. The 26S proteasome is comprised of one 20S core particle and two 19S regulatory particles (RPs). The RPN12a protein, a non-ATPase subunit of the 19S RP, was previously shown to be involved in cytokinin signaling in Arabidopsis. To further investigate cellular roles of RPN12a, RNAi transgenic plants of RPN12a were constructed. As expected, the 35S:RNAi-RPN12a plants showed cytokinin signaling defective phenotypes, including abnormal formation of leaves and inflorescences. Furthermore, RNAi knock-down transgenic plants exhibited additional unique phenotypes, including concave and heart-shape cotyledons, triple cotyledons, irregular and clustered guard cells, and defects in phyllotaxy, all of which are typical for defective cytokinin signaling. We next examined the mRNA level of cytokinin signaling components, including type-A ARRs, type-B ARRs, and CRFs. The expression of type-A ARRs, encoding negative regulators of cytokinin signaling, was markedly reduced in 35S:RNAi-RPN12a transgenic plants relative to that in wild type plants, while type-B ARRs and CRFs were unaffected. Our results also indicate that in vivo stability of the ARR5 protein, a negative regulator of cytokinin signaling, is mediated by the 26S proteasome complex. These results suggest that RPN12a participates in feedback inhibitory mechanism of cytokinin signaling through modulation of the abundance of ARR5 protein in Arabidopsis.