• Archives of Pharmacal Research
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• 제28권1호
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• pp.68-72
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• 2005

Pseudolaric acid B is a major compound found in the bark of Pseudolarix kaempferi Gordon. In our study, pseudolaric acid B inhibited growth of human melanoma cells, A375-S2 in a time and dose-dependent manner. A375-S2 cells treated with pseudolaric acid B showed typical characteristics of apoptosis including morphologic changes, DNA fragmentation, sub-diploid peak in flow cytometry, cleavage of poly-ADP ribose polymerase (PARP) and degradation of inhibitor of caspase-activated DNase (ICAD). P53 protein expression was upregulated while cells were arrested at the $G_2/M$ phase of the cell cycle. There was a decrease in the expression of anti-apoptotic Bcl-2 and Bcl-xL proteins, whereas pro-apoptotic Bax was increased. The two classical caspase substrates, PARP and ICAD, were both decreased in a time-dependent manner, indicating the activation of downstream caspases.

• 한국식품과학회지
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• 제38권3호
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• pp.419-426
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• 2006

전국 각지에서 채집한 토양에서 분리한 25종의 내열성 균주 중 내열성 단백질 기수분해효소 활성을 갖는 균주 strain JE 375를 선별하였다. 본 균주는 gram 양성 간균의 특징을 나타냈으며 Bergey's Manual of Systematic Bacteriology와 Biochemical Tests for Identification of Medical Bacteria에 준하여 생화학적 특성을 검토한 결과 catalase 양성, 포자형성, motility 양성, glucose 발효, mannitol 발효, xylose 산화, hemolysis ${\beta}$균임을 보아 Bacillus sp.으로 추정 되었다. Strain JE 375의 whole cell fatty acid를 gas chromatography로 분석한 결과 $C_{15:0}$ iso 26.17%, $C_{16:0}$ iso 13.01%, $C_{17:0}$ iso 30.19%로 분석되어 Bacillus 계열로 동정되었다. 16S rDNA sequence분석 결과 strain JE 375는 Bacillus caldoxylolyticus와 sequence가 97.6% 일치하는 유사성을 보였으나 부분적으로 sequence의 차이가 있고 gene bank data base상에서 16S rDNA sequence가 일치하는 균주는 검색되지 않았다. 이 같은 실험 결과에 따라 strain JE 375는 기존에 발표되지 않은 새로운 균주로 판단되어 Bacillus sp. JE 375로 명명하였다. Bacillus sp. JE 375은 tryptone 1%, yeast extract 0.5%, NaCl 1%, maltose 1%의 배지조성분과 배양 온도 $65^{\circ}C$에서 20시간 동안 배양하였을 때 최대의 단백질 분해 효소를 생산하였다. Bacillus sp. JE 375로부터 단백질 분해 효소를 acetone으로 침전시키고 DEAE-sepharose column chromatography를 통하여 효소를 정제하여 SDS-PAGE를 통해 확인한 결과 55 kDa 크기의 band를 확인할 수 있었다. 이 효소의 최적 배양 온도는 $65^{\circ}C$이었으며 배지의 최적 pH는 6.5로 나타났다. pH에 대한 안정성은 중성 부근의 pH에서 효소 활성의 안정성이 높게 나타났다. 본 효소의 반응 조건을 검토한 결과 중성 조건에서 안정하였으며, $60^{\circ}C$의 고온에서 활성을 가졌다. 효소 활성은 1 mM $CaCl_2$ 첨가에 의해 증가하였다.

• 생명과학회지
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• 제24권8호
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• pp.903-909
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• 2014

본 연구에서는 제주도 한라산에 광범위하게 자생하는 제주조릿대의 항암 제제로써의 이용 가능성을 평가하기 위하여 6개 암세포(A549, MCF-7, HepG-2, Hela, HCT116, A375)를 대상으로 세포주기 교란 작용 및 자연사멸 효과를 탐색하였다. MTT 분석 결과 제주조릿대가 다양한 암세포의 증식을 효과적으로 저해하였으며, sub-G1기의 증가와 DNA 분절로 인한 자연사멸 증가에 산화질소가 연관성이 있었다. 이와 별개로 제주조릿대는 세포주기의 장애를 야기하여 암세포의 생장을 억제하는 것으로 나타나 상기의 결과들로 예측하여 볼 때 제주조릿대를 항암 활성을 지닌 소재로 활용 가능할 것이며, 향후 정확한 자연사멸기전 규명을 위한 연구가 진행되어야 할 것이다.

• 대한화학회지
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• 제51권4호
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• pp.331-338
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• 2007

분석 시약으로 2-hydroxy-3-methoxy benzaldehyde thiosemicarbazone (HMBATSC)를 사용하여 코발트 (II) 정량을 위하여 빠르고 간단하고 민감한 분광광도법을 개발하였다. 액상에서 금속이온이 pH 6.0에서 HMBATSC와 갈색의 착물을 형성하였다. 이 착물은 375 nm와 390 nm에서 두 개의 최대 흡수 피크를 보였다. 375 nm에서 많은 흡 수를 보였고, 반면 390 nm에서는 분명한 흡수를 보이지 않았다. Beer's 법칙에서 Co(II)는 0.059-2.357 μg ml-1 범위를 보였다. 이 방법의 몰 흡수와 Sandall's 민감도는 각각 2.74×104 l mol-1 cm-1와 0.0024 μg cm-2 였다. 여러 가지 다른 이온들에 간섭에 대해서도 연구하였다. 화학양론적으로 착체는 1:2 [Co(II)- HMBATSC] 이 였다. 이차 유도 분광광도 법에 의한 Co(II)의 정량방법에 대해서도 제안하였다. 제안된 방법을 alloy steels, 비타민 B12와 생물학 시료에 있는 Co(II)의 정량에 적용하였다.

• 대한전자공학회논문지
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• 제26권4호
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• pp.62-66
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• 1989

$Bi_2S_3$다결정과 $Bi_2S_3$ 무정형 박막은 증착방법에 의해서 성장되었다. 측정된 격자상수들은 기판온도 $210^{circ}C$에서 $a=1.708{\AA},\;b=3.943{\AA} 그리고 $c=3.943{\AA}$이었으며, orthorhombic 구조를 가진것으로 나타내었다. 다결정 박막 $Bi_2S_3$energy은 $289^{circ}C$ 에서 1.375eV로 측정되었다. 674nm의 중심에서는 변화된 광자흡수 구조로 생각되어졌다.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2014년도 제46회 동계 정기학술대회 초록집
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• pp.375-375
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• 2014

Layered semiconductor materials can be a promising candidate for large-area thin film transistors (TFTs) due to their relatively high mobility, low-power switching, mechanically flexibility, optically transparency, and amenability to a low-cost, large-area growth technique like thermal chemical vapor deposition (CVD). Unlike 2D graphene, series of transition metal dichalcogenides (TMDCs), $MX_2$ (M=Ta, Mo, W, X=S, Se, Te), have a finite bandgap (1~2 eV), which makes them highly attractive for electronics switching devices. Recently, 2D $MoS_2$ materials can be expected as next generation high-mobility thin-film transistors for OLED and LCD backplane. In this paper, we investigate in detail the electrical characteristics of 2D layered $MoS_2$ local bottom-gated transistor with the same device structure of the conventional thin film transistor, and expect the feasibility of display application.

• Asian Pacific Journal of Cancer Prevention
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• 제13권4호
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• pp.1611-1615
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• 2012

Triptolide, a diterpenoid obtained from Tripteryglum wilfordii Hook.f, has attracted interest for its antitumor activities against human tumor cell lines in recent years. This report focuses on anti-proliferative and pro-apoptotic activities in human melanoma A375 cells assessed by CCK8 assay, Hoechst 33258 staining and flow cytometry. In addition, triptolide-induced arrest in the S phase was also observed. Caspase assays showed the apoptosis induced by triptolide was caspase-dependent and probably through intrinsic apoptotic pathways. Furthermore, expression of NF-${\kappa}B$ (p65) and its downstream factors such as Bcl-2, Bcl-$X_L$ was down-regulated. Taken together, the data indicate that triptolide inhibits A375 cells proliferation and induces apoptosis by a caspase-dependent pathway and through a NF-${\kappa}B$-mediated mechanism.

• Journal of Computing Science and Engineering
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• 제2권4호
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• pp.375-393
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• 2008

The dynamic cast operation allows flexibility in the design and use of data management facilities in object-oriented programs. Dynamic cast has an important role in the implementation of the Data Management Services (DMS) of the Mission Data System Project (MDS), the Jet Propulsion Laboratory's experimental work for providing a state-based and goal-oriented unified architecture for testing and development of mission software. DMS is responsible for the storage and transport of control and scientific data in a remote autonomous spacecraft. Like similar operators in other languages, the C++ dynamic cast operator does not provide the timing guarantees needed for hard real-time embedded systems. In a recent study, Gibbs and Stroustrup (G&S) devised a dynamic cast implementation strategy that guarantees fast constant-time performance. This paper presents the definition and application of a cosimulation framework to formally verify and evaluate the G&S fast dynamic casting scheme and its applicability in the Mission Data System DMS application. We describe the systematic process of model-based simulation and analysis that has led to performance improvement of the G&S algorithm's heuristics by about a factor of 2. In this work we introduce and apply a library for extracting semantic information from C++ source code that helps us deliver a practical and verifiable implementation of the fast dynamic casting algorithm.

• Restorative Dentistry and Endodontics
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• 제6권1호
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• pp.63-70
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• 1980

This study was to determine the incidence of pulp stone in each tooth by means of analyzing 375 orthopantomograph taken from 375 patients (252 male), 125 female). The pictures were grouped by age, 20s, 30s, 40s, 50s, and sex. The results were as follows 1. In the group of 20s, male showed the highest incidence in the first molar (40~50%) and the second highest was in the 2nd molar about 35%~50%. In the female group, the first molar and the second molar showed 25%~40%. 2. In the group of 30s, the highest value was found on the first molar and the next was the 2nd molar which appeared 30%~40% in male. In the female, the first molar was 55%~70% and the 2nd molar 30%~50% 3. In the group of 40s, the first molar represented highest value about 45%~60% and the 2nd molar was 40~60% in the male. Female group also showed in the first molar 30%~50% and the second molar 25~30%. 4. In the group of 50s, the highest incidence was 40%~60% on the first molar and next was 35%~50% on the second molar in male, in femalegroup showed 30%~50% on the first molar and 25%~50% on the second molar. 5. The incidence in upper was higher than that of lower. 6. Comparison between right and left showed right was slightly higher than left side.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.375.2-375.2
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• 2002

S.deltoides (Compositae) distributed widely in Korea. China. It is edible as a food additive. but there has been no study on chemical constituents. Therefore. we isolated nine compounds from S.deltoldes. On the basis of spectroscopic evidence. the structure of these compounds were characterized as lupeol( 1), $\alpha$-amyrin(2).$\beta$-amyrin (3), ursolic acid(4), nonacosanol(5), nonacosanoic acid(6). mixture of $\beta$-sitosterol. stigmasterol and campesterol (7), $\beta$-sitosteryl-3-O-$\beta$-D-glucopyranoside(8), stigmasteryI-3-O-$\beta$-D-glucopyranoside(9). (omitted)