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http://dx.doi.org/10.5352/JLS.2014.24.8.903

Antiproliferative and Apoptotic Effects of Sasa quelpaertensis Nakai in Human Cancer Cells  

Kim, Ji Hye (Toxicology Laboratory, Faculty of Biotechnology, College of Applied Life Science, SARI, Jeju National University)
Kim, Min Young (Research Institute for Subtropical Agriculture and Biotechnology, Jeju National University)
Publication Information
Journal of Life Science / v.24, no.8, 2014 , pp. 903-909 More about this Journal
Abstract
Plants are an invaluable source of potential new anti-cancer drugs. Sasa quelpaertensis Nakai (Korean name, Jeju-Joritdae) is one of these plants with medical value, which is a bamboo grass widely distributed in Mt. Halla on Jeju Island, Korea. Here, we investigated the apoptotic effects of S. quelpaertensis leaf extracts in six human cancer cell lines (A549, MCF-7, HepG-2, Hela, HCT116 and A375). MTT assay signified the antiproliferative nature of S. quelpaertensis extracts against all tested cancer cells: S. quelpaertensis displayed slight cytotoxicity against A549, MCF-7 and HepG-2 cells, whereas it was exclusively cytotoxic to Hela, HCT116 and A375 cells. Apoptotic cells were evaluated using PI staining of DNA fragmentation by flow cytometry (sub-G1 peak). PI staining indicated increasing accumulation of Hela, HCT116 and A375 cells at sub-G1 phase. Further events like generation of nitric oxide ($NO^{\bullet}$) were accompanied in the S. quelpaertensis Nakai-induced apoptosis. Augmented $NO^{\bullet}$ generation resulted in the DNA fragmentation of Hela, HCT116 and A375 cells by treatment with S. quelpaertensis leaf extracts. These results suggest that S. quelpaertensis may be a potential natural resource for treating cancer cell. To identify the exact mechanisms of molecular mechanism of S. quelpaertensis induced apoptosis awaits further investigation.
Keywords
Apoptosis; human cancer cells; nitric oxide; Sasa quelpaertensis Nakai;
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