• Toxicological Research
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• 제34권1호
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• pp.13-21
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• 2018

Anthocyanins are naturally occurring water-soluble polyphenolic pigments in plants that have been shown to protect against cardiovascular diseases, and certain cancers, as well as other chronic human disorders. However, the anti-obesity effects of anthocyanins are not fully understood. In this study, we investigated the effects of anthocyanins isolated from the fruit of Vitis coignetiae Pulliat on the adipogenesis of 3T3-L1 preadipocytes. Our data indicated that anthocyanins attenuated the terminal differentiation of 3T3-L1 preadipocytes, as confirmed by a decrease in the number of lipid droplets, lipid content, and triglyceride production. During this process, anthocyanins effectively enhanced the activation of the AMP-activated protein kinase (AMPK); however, this phenomenon was inhibited by the co-treatment of compound C, an inhibitor of AMPK. Anthocyanins also inhibited the expression of adipogenic transcription factors, including peroxisome proliferator-activated receptor-${\gamma}$, CCAAT/enhancer-binding protein a and b, and sterol regulatory element-binding protein-1c. In addition, anthocyanins were found to potently inhibit the expression of adipocyte-specific genes, including adipocyte fatty acid-binding protein, leptin, and fatty acid synthase. These results indicate that anthocyanins have potent anti-obesity effects due to the inhibition of adipocyte differentiation and adipogenesis, and thus may have applications as a potential source for an anti-obesity functional food agent.

• 한국약용작물학회지
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• 제17권1호
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• pp.68-74
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• 2009

Vitis amurensis (VA; Vitaceae) has long been used in oriental herbal medicine. It has been reported that roots and seeds of VA have anti-inflammatory and antioxidant effects. In the present study, the protective effect of ethanol extract from stems and leaves of VA on hydrogen peroxide (${H_2}{O_2}$) (100 ${\mu}M$)-induced neuronal cell damage was examined in primary cultured rat cortical neurons. VA (10-100 ${\mu}g$/ml) concentration-dependently inhibited ${H_2}{O_2}$-induced apoptotic neuronal cell death measured by 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay and Hoechst 33342 staining. VA inhibited ${H_2}{O_2}$-induced elevation of intracellular $Ca^{2+}$ concentration (${[Ca^{2+}]}_i$) and generation of reactive oxygen species (ROS), which were measured by fluorescent dyes. Pretreatment of VA also prevented glutamate release into medium induced by 100 ${\mu}M$ ${H_2}{O_2}$, which was measured by HPLC. These results suggest that VA showed a neuroprotective effect on ${H_2}{O_2}$-induced neuronal cell death by interfering with ${H_2}{O_2}$-induced elevation of ${[Ca^{2+}]}_i$, glutamate release, and ROS generation. This has a significant meaning of finding a new pharmacological activity of stems and leaves of VA in the CNS.

• Bulletin of the Korean Chemical Society
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• 제34권6호
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• pp.1906-1908
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• 2013

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2002년도 생물공학의 동향 (X)
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• pp.285-288
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• 2002

This study was conducted to optimize phytohormones combination and concentration on resveratrol production of Vitis vinifera callus cultures. TDZ was so effective for callus proliferation and resveratrol production and can be expected as a stimulus to the cells which keep the ability of producing resveratrol. We optimized the hormone combination of NAA 0.5 mg/L and TDZ 2 mg/L to stimulate resveratrol production very effectively. And callus under MS medium with the optimized phytohormones combination was treated by fungal elicitor, Botrytis.

• Korean Journal of Acupuncture
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• 제24권1호
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• pp.161-170
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• 2007

Objectives : In this study, we determinated protective effects of Vitis labrusca Root herbal-acupuncture solution (VLHA) against atherosclerosis and cardiovascular disease induced by oxidative damage and cytochrome P450 (CYP) induction. Methods : Antioxidant capacity of VLHA were evaluated by the ${\beta}-carotene/linoleic$ acid emulsion system and FeCl2-ascorbic acid stimulted lipid peroxidation in rat liver homogenate, respectively. Scavenging effects of VLHA on ONOO- and NO were measured by DHR 123, DAF-2 assay. Furthermore, we measured inhibitory activity of VLHA on CYP 2E1 in rat liver microsome using by Aniline assay. Results : VLHA exhibited potent inhibition rate(74%) on FeCl2-ascorbic acid induced lipid peroxidation, and revealed a strong scavenging effects on ONOO- and NO. In addition, VLHA showed inhibitory effect on CYP 2E1. Conclusions : The present study concludes that our results suggest that VLHA could be used as a good source in the prevention of atherosclerosis and alcoholic liver injury.

• 식물병연구
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• 제12권1호
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• pp.15-19
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• 2006

포도 갈색무늬병균의 침입부위를 알고자 잎의 앞면과 뒷면에 병원균을 접종한 결과 포도 잎의 앞면에 병원균을 접종한 처리구는 전혀 침입이 없었으며, 같은 잎의 뒷면에 병원균을 접종한 모든 처리구에서는 접종 15일 후에, 접종 지점 수와 동일한 수의 병반이 형성되었다. 병원균 접종 후에 발병에 소요되는 기간을 조사한 결과, 포도 잎의 뒷면에 접종한 경우, 접종 8일 후부터 병반이 나타나기 시작하여, 접종 10일 후에는 거의 모든 접종 지점에 병반이 나타났다. 갈색무늬병에 감수성인 캠벨얼리 품종에서는 잎 뒷면에 부착한 분생포자가 발아하여, 기공을 통하여 직접 식물체 조직 속으로 쉽게 침입하는 것을 관찰할 수 있었다. 반면에 저항성인 거봉 품종에서는 분생포자가 기공 근처에서 발아하였어도 기공을 쉽게 인식하여 찾아가지 못하거나, 기공을 찾아도 침입을 하지 못하고 기공을 넘어서 계속 발아하기도 하였다. 또한 발아관이 기공에 도달하는 도중에 기공 주변을 감싸기도 하고 공변세포에 이르러서도 쉽게 기공을 통해 조직 속으로 침입하지 못하였다. 심지어 어렵게 기공을 인식하여 침입을 했어도 계속 발아하면서 감수성 품종과 달리 조직 속으로 침입하지 않고 다시 기공 밖으로 나오는 모습이 관찰되기도 하였다. 갈색무늬병에 감수성 품종과 저항성 품종에서의 단위면적당 기공 수를 조사한 결과, 감수성 품종인 캠벨얼리 품종이 저항성 품종인 거봉의 기공 수보다 많았고, 그 크기는 거봉 품종이 컸다. 따라서 갈색무늬병균은 단위면적당 기공 수의 다소가 침입을 좌우하고, 그 크기에 의해 좌우되지는 않는 것으로 추정된다. 저항성인 거봉 품종의 기공은 전체적으로 식물체의 표면과 기공을 구성하는 공변세포와의 높이 차이가 거의 없었던 것이 형태적으로 명확하게 관찰되는 반면, 감수성인 캠벨얼리 품종의 기공은 전체적으로 식물체의 표면과 기공을 구성하는 공변세포와의 높이 차이가 현저한 것을 주사전자현미경과 광학현미경을 이용하여 명확하게 관찰하였다.

• 동의생리병리학회지
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• 제21권6호
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• pp.1411-1414
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• 2007

According to the report of Korea National Statistical Office in 2007, cerebral vascular disease is second cause of deaths in Korean. Cerebral ischemia is one of the main reason of cerebral vascular diseases. To evaluate the neuroprotective effect of heyneanol A against cerebral ischemia, we used the middle cerebral artery occlusion model (MCAO). Heyneanol A from root of vitis amurensis Rup is a tetramer of resveratrol as known anti-oxidant and anti-cancer agent. Although the effects of resveratrol in the various fields have been well established, little is known of the effects of heyneanol A. In this study, heyneanol A reduced infarction and edema volume by 33.5% and 57% compared with control groups (vehicle), respectively. Also, neurological score was decreased by heyneanol A. It's effects were more potent than resveratol. Taken together, these results exerted that heyneanol A has a neuroprotective effect against cerebral ischemia.

• 한국자원식물학회지
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• 제20권2호
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• pp.177-184
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• 2007

새로운 기능성 소재를 탐색하기 위하여, 13종의 식용 및 약용식물의 종자추출물을 조제하여 생리활성물질 함량, 항산화활성 그리고 tyrosinase 억제활성을 분석하였다. 총 폴리페놀 및 플라보노이드 함량은 주목에서 각각 $57.51mg{\cdot}g^{-1}$과 $7.98mg{\cdot}g^{-1}$으로 가장 높게 나타났다. 종자추출물의 DPPH 라디칼 소거 활성은 머루$\times$'캠벨얼리' 교배종에서 $RC_{50}$이 $0.03mg{\cdot}ml^{-1}$로 가장 높았고, ABTS 라디칼 소거활성은 고음나무 종자에서 $RC_{50}$이 $0.109mg{\cdot}ml^{-1}$로 가장 높게 나타났다. Ferric thiocyanate(FTC) 방법으로 linoleic acid에 대한 과산화 억제효과를 조사한 결과, 감나무, 고욤나무, 당후박나무, 쪽동백나무, 머루, 머루$\times$'캠벨얼리' 교배종, 묏대추나무, 대추나무의 종자추출물에서 BHT와 유사한 수준의 억제활성을 보였다. 종자추출물의 tyrosinase 억제활성은 감나무, 고욤나무, 탱자나무, 벚나무, 묏대추나무, 대추나무에서만 나타났으며, 특히 고음나무에서 가장 높았다.

• 반도체공학회 논문지
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• 제1권1호
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• pp.1-8
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• 2023

본 논문에서는 High-Level Synthesis(HLS)을 이용하여, 차세대 양자내성암호인 Crystals-Kyber를 하드웨어 가속기로 설계하여 FPGA에 구현하였으며, 성능 분석결과 우수성을 제시한다. Crystals-Kyber 알고리즘을 Vitis HLS 에서 제공하는 여러 Directive 를 활용해서 최적화 설계를 진행하고, AXI Interface 를 구성하여 FPGA-기반 양자내성암호 하드웨어 가속기를 설계하였다. Vivado 툴을 이용해서 IP Block Design 를수행하고 ZYNQ ZCU106 FPGA 에 구현하였다. 최종적으로 PYNQ 프레임워크에서 Python 코드로 동영상 촬영 및 H.264 압축을 진행한 후, FPGA 에 구현한 Crystals-Kyber 하드웨어 가속기를 사용해서 동영상 암호화 및 복호화 처리를 가속화하였다.

• The Plant Pathology Journal
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• 제19권5호
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• pp.235-238
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• 2003

The resistance to crown gall in grape rootstocks was evaluated by inoculating cuttings from 27 grape rootstock varieties with Agrobacterium vitis strain Cheonan 493. Thmors were formed in all varieties of grape rootstocks tested in this study. The symptoms were observed in the stems of all plants tested including '196-17' and '41B'. Based on the measurement of tumor weight on the stems of grape rootstocks, '779P' was found to be higbly susceptible to crown gall. While some varieties such as 'Gloire', '140R', '101-14M', '3309C', and '333EM' were found to be relatively resistance to crown gall. Varieties such as '99R', '1447P', 'Rupestris du lot', 'll0R', 'Freedom', and '41B' were also found to be susceptibl~ to crown gall. Other~ varieties including 'l103P', 'Teleki. 5C', '420A', 'Golia', and '5BB' were found to be moderately susceptible.