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Protective Effect of Vitis amurensis Stems and Leaves Extract on Hydrogen Peroxide-induced Oxidative Neuronal Cell Damage in Cultured Neurons  

Kim, Joo-Youn (College of Veterinary Medicine, Chungbuk National University)
Ju, Hyun-Soo (College of Veterinary Medicine, Chungbuk National University)
Ban, Ju-Yeon (Department of Pharmacology, College of Medicine, Kyung Hee University)
Song, Kyung-Sik (College of Agriculture and Life-Sciences, Kyungpook National University)
Bae, Ki-Hwan (College of Pharmacy, Chungnam National University)
Seong, Yeon-Hee (College of Veterinary Medicine, Chungbuk National University)
Publication Information
Korean Journal of Medicinal Crop Science / v.17, no.1, 2009 , pp. 68-74 More about this Journal
Abstract
Vitis amurensis (VA; Vitaceae) has long been used in oriental herbal medicine. It has been reported that roots and seeds of VA have anti-inflammatory and antioxidant effects. In the present study, the protective effect of ethanol extract from stems and leaves of VA on hydrogen peroxide (${H_2}{O_2}$) (100 ${\mu}M$)-induced neuronal cell damage was examined in primary cultured rat cortical neurons. VA (10-100 ${\mu}g$/ml) concentration-dependently inhibited ${H_2}{O_2}$-induced apoptotic neuronal cell death measured by 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay and Hoechst 33342 staining. VA inhibited ${H_2}{O_2}$-induced elevation of intracellular $Ca^{2+}$ concentration (${[Ca^{2+}]}_i$) and generation of reactive oxygen species (ROS), which were measured by fluorescent dyes. Pretreatment of VA also prevented glutamate release into medium induced by 100 ${\mu}M$ ${H_2}{O_2}$, which was measured by HPLC. These results suggest that VA showed a neuroprotective effect on ${H_2}{O_2}$-induced neuronal cell death by interfering with ${H_2}{O_2}$-induced elevation of ${[Ca^{2+}]}_i$, glutamate release, and ROS generation. This has a significant meaning of finding a new pharmacological activity of stems and leaves of VA in the CNS.
Keywords
Vitis amurensis; Hydrogen Peroxide; Neurotoxicity; Neuroprotection; Anti-oxidant;
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Times Cited By KSCI : 2  (Citation Analysis)
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