• 생약학회지
• /
• 제28권4호
• /
• pp.179-184
• /
• 1997

To search for useful antioxidants from plant materials, we investigated the antioxidative activity in the methanol extracts of 30 Chinese medicinal plants using DPPH method. The highest activity $(RC_{50}:\;12\;{\mu}g)$ was showed in the methanol extract of Rosa rugosa, followed by Potentilla fruticosa $(14\;{\mu}g)$, P. fragarioides $(16\;{\mu}g)$. And Geum aleppicum $(18\;{\mu}g)$. From the leaves of R. rugosa, two antioxidative compounds were isolated by a bioassay guided purification and identified as isoquercitrin and ${\beta}-glucogallin$ on the basis of $^1H-,\;^{13}C-\;NMR$, and FAB-MS data. ${\beta}-Glucogallin$ is the first report in this plant. The DPPH radical scavenging activity of ${\beta}-glucogallin\;(RC_{50}:\;8.5\;{\mu}g)$ was more effective than those of ${\alpha}-tocopherol\;(12\;{\mu}g)$ and BHA $(14\;{\mu}g)$.

• 한국식품위생안전성학회지
• /
• 제10권3호
• /
• pp.181-187
• /
• 1995

Water extract, and methanol extract, its chloroform and hexane fractions, and estragole from Agastache rugosa O. Kuntze were tested to find the inhibition effect on the growth of several microorganisms. The organisms used were Escherichia coli ATCC 1129, Staphylococcus aureus IAM 1011, Vibrio parahaemolyticus WP, Bacillus subtilis ATCC6633, Aspergillus oryzae KFCC 890, Aspergillus niger KCCM 11240. Water and methanol extracts at the concentration of 0.5%, and chloroform and hexane fraction at the concentration of 0.05% inhibited the growth of microorganisms from 1/5 to 2/3 of the control group. Estragole identified from the hexane faction as a major component, its authentic compound completely inhibited the growth of Vibrio parahaemolyticus completely at the concentration of 0.03%, and the other bacteria were at 0.05%.

• Natural Product Sciences
• /
• 제7권4호
• /
• pp.120-123
• /
• 2001

To develop systems for economic production of useful essential oil compounds, callus was induced from the seedlings of Agastache rugosa and cultured on MS medium. The volatile oil fraction was extracted from the callus and investigated by mean of GC-MS. The composition of the oil was compared with that of the mother plant. As a result, sixty five compounds including ferruginol were identified in the essential oil fraction. The main component of the oil from the leaves of Agastache rugosa was methyl chavichol (53.6%). Methyl jasmonate and jasmonic acid were added to the culturing cell suspension, separately and the composition of induced oil were compared. The oils from cultured cells treated with jasmonates showed considerably different patterns. Especially, the peak of estragole was found in callus oil after treatment with methyl jasmonate as though the amount was limited to 0.58%. In general, the TIC pattern of GC-MS of the callus oil became more similar to the oil from the leaves after elicitation.

• 한국자원식물학회지
• /
• 제20권3호
• /
• pp.255-257
• /
• 2007

This study was conducted to investigate the effect of sta-green and activated carbon on leaf and stem growth of Agastache rugosa as it is affected by different amounts of sta-green and activated carbon. The results obtained are summarized as follows. Growth characteristics including plant height and leaf number were the highest when treated with 30% of sta-green. The weight of fresh leaf and stem of Agastaches rugosa was very low in control. Also, fresh weight of Angelica acutiloba was higher in 10% treatment of activated carbon. However, when the plants were grown in 10% activated carbon. all these promoters were the biggest. Sta-green and activated carbon can be utilized as a soil conditioner in agricultural crop areas.

• Ocean Science Journal
• /
• 제40권4호
• /
• pp.209-212
• /
• 2005

In the present study, we have tested the effects of 21 salt marsh plants on cell proliferation of mouse immune cells (spleen and thymus) using MTT assay in culture. The methanolic extracts of six salt marsh plants (Rosa rugosa, Ixeris tamagawaensis, Artemisia capillaris, Tetragonia tetragonoides, Erigeron annus, and Glehnia littoralis) showed very powerful suppressive effects of mouse immune cell death and significant activities of cell proliferation in vitro. Especially, the methanolic extract of Rosa rugosa was found to have fifteen times compared to the control treatment, demonstrating that Rosa rugosa may have a potent stimulation effect on immune cell proliferation. These results suggest that several salt marsh plants including Rosa rugosa could be useful for further study as an immunomodulating agent.

• Journal of the Korean Wood Science and Technology
• /
• 제46권1호
• /
• pp.38-47
• /
• 2018

본 연구에서는 해당화 부위별 열수추출물에 대한 항산화 활성을 분석하였다. 해당화의 부위별 추출물의 총 페놀성 화합물과 총 플라보노이드 함량은 잎에서 가장 높았으며, 각각 107.29, 24.28 mg/g로 나타났다. DPPH와 ABTS 라디칼 소거능은 꽃, 잎, 씨, 열매, 과육 추출물 순으로 활성이 높았으며, 꽃 추출물의 DPPH, ABTS 라디칼 소거능은 각각 $IC_{50}=0.87$, $0.27mg/m{\ell}$로 나타났다. 항산화 활성이 가장 높았던 꽃과 잎 추출물에서는 gallic acid가 각각 4.51, 0.97 mg/g로 다른 부위별 열수추출물에서보다 높았다. 해당화 부위별 열수추출물을 분획한 결과(A-F), 꽃 추출물의 C 분획물에서 높은 항산화 활성을 확인하였다. 이것은 총 페놀성 화합물과 총 플라보노이드 함량(3305.43, 878.42 mg/g)에 의한 것이며, p-value < 0.001에서 총 페놀성 화합물과 총 플라보노이드의 함량이 증가할수록 라디칼 소거능이 증가하는 상관관계 결과와 일치하였다. 따라서 해당화 열수추출물은 식품, 화장품, 의약품 산업 등에서 천연 항산화제로 사용가능할 것이다.

• 생물환경조절학회지
• /
• 제26권4호
• /
• pp.249-257
• /
• 2017

배초향은 항동맥경화나 항박테리아의 특성을 가지는 한약재에 널리 사용되는 영년생 약용식물이다. 연구의 목적은 수경재배에서 배양액의 종류와 PPFD값에 따른 배초향의 생장 및 항산화 물질의 변화를 조사하는 것이다. 배초향은 주야간 16:8 시간의 일장조건에서 150과 $200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD 조건과 일본원시(HES), 서울시립대(UOS), 유럽채소연구소(EVR), 오오츠카 배양액(OTS)을 이용하여 6주간 재배하였다. OTS 배양액조건에서 자란 배초향의 지상부 및 지하부 건물중은 다른 배양액 처리구와 비교하여 유의적으로 높았다. 배초향의 틸리아닌 함량은 OTS 처리에서 가장 높았으며 다음으로 EVR, HES, UOS 순서로 낮아졌다. 총 아카세틴의 함량은 EVR처리에서 가장 높았으나 OTS처리와는 유의적 차이를 보이지 않았다. 또한 $200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD 조건에서 자란 배초향은 PPFD 150처리구와 비교하여 유의적으로 생체중과 건물중이 증가하였으며 기능성 물질은 틸리아닌과 아카세틴의 함량도 높았다. 본 연구는 수경재배 방식을 이용하여 식물공장에서 배초향을 재배할 경우 $200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD 조건과 OTS 배양액 조건에서 경제적인 광원조건으로 최적 바이오매스 생산량과 틸라아닌과 아카세틴의 함량을 증가시킬 수 있을 것으로 제안한다.

• 한국자원식물학회지
• /
• 제23권2호
• /
• pp.138-144
• /
• 2010

최근 한약추출박은 배출되는 양이 많아 쓰레기화 되어 환경을 크게 오염시키고 있다. 이러한 한약추출박을 퇴비로 재활용하기 위한 기초시험으로 첩약(貼藥)인 '연령고본단(延齡固本丹)'의 한약추출박 성분을 분석하였고, 여기에 배초향을 재배하여 비료로 시용한 후 생육특성과 전초 수량을 조사한 결과는 다음과 같다. 1. 한약추출박의 분석 성분 중 유기물은 89.6%가 함유되어 있었고, 염분은 0.1%로 약초의 생장에 지장이 없는 공정규격(1.0%)이하의 범위였다. 약초의 생장에 직접적으로 필요한 비료 3요소 중 질소는 2.8%가 함유되어 있었고, 인산은 0.6%, 그리고 칼륨은 0.5%가 함유되어 있었다. 미량성분으로 식물체의 영양분이 될 수 있는 성분은 아연과 구리 등이 함유되어있었다. 이러한 분석결과, 한약추출박 성분은 유기물과 질소, 인산, 가리, 미량요소 등이 골고루 함유되어 유기질 비료로 사용 될 수 있을 것으로 본다. 2. 한약추출박의 시용에 따른 배초향의 생육은 한약추출박 시용이 무시용보다 초장과 경직경이 크고, 엽수와 가지수가 많아 생장이 양호 하였고, 수량도 전초 1주중이 무거워 증수되는 경향이었다. 한약추출박의 시용농도는 30~50 g의 시용에서 효과가 인정되었으므로 추출박 30 g의 농도가 적정 시용 농도로 판단된다. 이상의 결과를 종합하여 볼 때 한약추출박은 배초향과 같은 전초이용 약초에 유기질 비료로 재활용 할 수 있을 것으로 본다.

• Journal of Applied Biological Chemistry
• /
• 제66권
• /
• pp.81-89
• /
• 2023

The purpose of this study is to demonstrate the potential enhancement of the flavonoid contents from Agastache rugosa, which can be obtained as raw materials for functional products in the food medicine industry by identifying important factors for efficient preparation to save costs and time in terms of economic factors. For this reason, response surface methodology using Box-Behnken design was used to optimize the extraction conditions for the maximum yield of seven major compounds from A. rugosa. The optimum conditions were obtained with an ethanol concentration of 60.0%, a temperature of 50 ℃, and an extraction time of 33.6 min, meaning that the regression analysis fits the experimental data well. Under these conditions, the seven major compounds 1-7 had observed values of 2.169, 2.135, 0.697, 2.485, 0.105, 1.247, and 0.551%, respectively. These results show that the observed values are in good agreement with the predicted values in the regression model. This process for optimization study exhibited a basic protocol for obtaining stable ingredients from A. rugosa that are appropriate for the development of effective functional products.

• Animal cells and systems
• /
• 제3권1호
• /
• pp.89-96
• /
• 1999

Nucleotide sequences of a 501 base-pair (bp) fragment in the mitochondrial cytochrome b (cyt b) gene were analyzed for 12 populations of Rana rugosa from Korea and Japan using a polymerase chain reaction (PCR) and direct silver sequencing. Two genetically distinct groups (type-A and type-B) were found in Korea. Type-A was found throughout most of South Korea and type-B was restricted to the mid-southeastern regions (Samchok, Yongdok, Chongsong and Pohang). But in the Tonghae population, both types were found. The level of mitochondrial DNA (mtDNA) sequence differences ranged from 0% to .0.8% among six populations of type-A, and 0 to 1.0% among 4 populations of type-B. However, sequence differences between type-A and type-B ranged from 5.4% to 6.6%, Using Kimura's two-parameter distance, the level of genetic sequence divergence between type-A and type-B was 6.7%. The Japanese R. rugosa was clustered very far from the Korean R. rugosa with 14.7%. In the neighbor-joining and UPGMA tree, all Korean samples were grouped, but subdivided into two types in 99% of the bootstrap iteration.