Browse > Article

Effects of Several Salt Marsh Plants on Mouse Spleen and Thymus Cell Proliferation Using MTT Assay  

Seo, Young-Wan (Division of Marine Environment and Biosciences, Korea Maritime University)
Lee, Hee-Jung (Research Institute of Marine Science and Technology(RIMST), Korea Maritime University)
Kim, You-Ah (Division of Marine Environment and Biosciences, Korea Maritime University)
Youn, Hyun-Joo (Department of Microbiology and School of Biotechnology & Biomedical Science, Inje University)
Lee, Burm-Jong (Department of Chemistry, Inje University)
Publication Information
Ocean Science Journal / v.40, no.4, 2005 , pp. 209-212 More about this Journal
Abstract
In the present study, we have tested the effects of 21 salt marsh plants on cell proliferation of mouse immune cells (spleen and thymus) using MTT assay in culture. The methanolic extracts of six salt marsh plants (Rosa rugosa, Ixeris tamagawaensis, Artemisia capillaris, Tetragonia tetragonoides, Erigeron annus, and Glehnia littoralis) showed very powerful suppressive effects of mouse immune cell death and significant activities of cell proliferation in vitro. Especially, the methanolic extract of Rosa rugosa was found to have fifteen times compared to the control treatment, demonstrating that Rosa rugosa may have a potent stimulation effect on immune cell proliferation. These results suggest that several salt marsh plants including Rosa rugosa could be useful for further study as an immunomodulating agent.
Keywords
salt marsh; spleen; thymus; MTT;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Lee, D.G., J.W. Hyun, K.A. Kang, J.O. Lee, S.H. Lee, B.J. Ha, J.M. Ha, E.Y. Lee, and J. H. Lee. 2004. Ulva lactica: A potential seaweed for tumor treatment and immune stimulation. Biotechnol. Bioeng., 9, 236-238
2 Lee, H. J., Y. A. Kim, J. W. Ahn, B. J. Lee, S. G. Moon, and Y. Seo. 2004. Screening of peroxynitrite and DPPH radical scavenging activities from salt marsh plants. Kor. J. Biotechnol. Bioeng., 19, 57-61
3 Liu, J. N., Y. Yoshida, M.Q. Wang, Y. Okai, and U. Yamashita. 1997. B cell stimulation activity of seaweed extracts. Int. J. Immunopharmaco., 19, 135-142   DOI   ScienceOn
4 Park, K. E., Y. A. Kim, H. A. Jung, H. J. Lee, J. W. Ahn, B. J. Lee, and Y. Seo. 2004. Three norisoprenoids from the brown alga Sargassum thunbergii. J. Kor. Chem. Soc., 48, 394-398   DOI
5 Seo, Y., H.J. Lee, K.E. Park, Y.A. Kim, J.W. Ahn, J.S. Yoo, and B.J. Lee. 2004. Peroxynitrite scavenging constituents from the brown alga Sargassum thunbergii. Biotechnol. Bioeng., 9, 212-216   DOI   ScienceOn
6 Wong, K.T. and B.K.H. Tan. 1996. In vitro cytotoxicity and immunomodulating property of Rhaphidophora korthalsii. J. Ethnopharmacol., 52, 53-57   DOI   ScienceOn
7 Koo, K.A., J.H. Kwak, K.R. Lee, O.P. Zee, E.R. Woo, H.K. Park, and H.J. Youn. 1994. Antitumor and immunomodulating activities of the polysaccharide fractions from Artemisia selengensis and Artemisia iwayomogi. Arch. Pharm. Res., 17, 371-374   DOI   ScienceOn
8 Park, J.H., J.K. Lee, H.S. Kim, S.T. Chung, J.H. Eom, K.A. Kim, S.J. Chung, S.Y. Paik, and H.Y. Oh. 2004. Immunomodulatory effect of caffeic acid phenethyl ester in Balb/c mice. Int. Immunopharmacol., 4, 429-436   DOI   ScienceOn
9 Shan, B.E., Y. Yoshida, E. Kuroda, and U. Yamashita. 1999. Immunomodulating activity of seaweed extract on human lymphocytes in vitro. Int. J. Immunopharmaco., 21, 59-70   DOI   ScienceOn
10 Hwang, J.S., H.K. Chung, E.K. Bae, A.Y. Lee, H.J. Ji, D.W. Park, H.J. Jung, C.W. Cho, D. S. Lee, K. R. Kee, and H. J. Youn. 2003. The polysaccharide fraction AIPI from Artemisia iwayomogi suppresses apoptotic death of the mouse spleen cells in culture. Arch. Pharm. Res., 26, 294-300   DOI   ScienceOn
11 Ohta, Y., S. Fukuda, A. Baba, H. Nagai, T. Taskuda, T. Sohda, and H. Makino. 1996. Immunomodulating and articular protecting activities of a new anti-rheumatic drug, TAK-603. Immunopharmacology, 34, 17-26   DOI   ScienceOn
12 Roitt, I., J. Brostoff, and D. Male. 1993. Immunology. Mosby, London. 423 p
13 Choi, H.K. and H.J. Kim. 1998. A distributional patten of halophytes around Shiwha lake in Korea. J. Ajou Nat. Sci., 3, 1-5
14 Mossman, T. 1983. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assay. J. Immunol. Methods, 65, 55-63   DOI   PUBMED   ScienceOn
15 Bang, M.A., H.A. Kim, and Y.J. Kim. 2002. Hypoglycemic and antioxidant effect of dietary hamcho powder in streptozotocininduced diabetic rats. J. Kor. Soc. Food Sci. Nutr., 31, 840-846   DOI
16 Fleisher, T.A. and J.B. Oliveira. 2004. Functional and molecular evaluation of lymphocytes., J. Allergy Clin. Immunol., 114, 227-234   DOI   ScienceOn
17 Han, S.K., S.M. Kim, and B.S. Pyo. 2003. Antioxidative effect of glasswort (Salicornia herbacea L.) on the lipid oxidation of pork. Kor. J. Food Sci. Ani. Resour., 23, 46-49
18 Jo, Y.C., J.H. Ahn, S.M. Chon, K.S. Lee, T.J. Bae, and D.S. Kang. 2002. Studies on pharmacological effects of glasswort (Salicornia herbacea L.). Kor. J. Medicinal Crop Sci., 10, 93-99
19 Ye, Y., H. Sun, X. Li, F. Chen, F. Qin, and Y. Pan. 2005. Four new C-21 steroidal glycosides from the roots of Stephanotis mucronata and their immunological activities. Steroids, 70, 791-797   DOI   ScienceOn
20 Slater, T.F., B. Sawyer, and U. Struli. 1963. Studies on succinatetetrazolium reductase systems III, points of coupling of four different tetrazolium salts. Biochem. Biophys. Acta., 77, 383-393   DOI   PUBMED