• 동의생리병리학회지
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• 제36권4호
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• pp.120-124
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• 2022

Actinidia polygama has long been used in traditional Korean medicine to treat rheumatoid arthritis and gout. Although numerous chemical compounds in the fruit extracts of A. polygama have been characterized and their role in inhibiting nitric oxide (NO) production has been reported, the anti-inflammatory properties of A. polygama extracts remain to be explored. In this study, we investigated the in-vivo effect of A. polygama extract on lipopolysaccharide (LPS)-induced inflammation in BV-2 microglial cell lines. We discovered that 100% ethyl alcohol extract of A. polygama effectively attenuates the release of NO and is superior to both water extract and 50% ethanol extract. Using MTT assay, western blot, and ELISA on LPS-induced BV-2 microglial cells lines, we established the ability of A. polygama extract to markedly suppress the expressions of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), and pro-inflammatory cytokines, such as tumor necrosis factor alpha and interleukin-6. These results reveal that the anti-inflammatory property of A. polygama in BV-2 microglial cells is due to the downregulation of iNOS, COX-2, MAPK protein, and pro-inflammatory cytokines.

• 한국조리학회지
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• 제22권2호
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• pp.125-135
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• 2016

본 연구는 개다래 분말을 0, 1, 3, 5, 7%를 첨가하여 머핀을 제조하고, 그 품질특성을 조사하였다. 머핀의 부피와 높이 및 비용적은 대조구가 개다래 첨가 머핀에 비해 더 높았으며, 중량은 시료간 유의한 차이가 없었다. 머핀의 pH는 개다래 분말 첨가량이 많아질수록 감소하였으며, 수분함량은 26.83~30.86% 범위로 개다래 분말 첨가량이 증가할수록 유의적으로 감소하였다(p<.001). 머핀의 색도는 개다래 분말 첨가량이 증가함에 따라 명도(L값)와 황색도(b값)는 감소하였으며, 적색도(a값은)는 증가하였다. Texture는 개다래 분말을 첨가할수록 경도, 탄력성, 씹힘성, 부서짐성이 증가하였으며, DPPH radical 소거능은 개다래 분말 7%가 가장 높게 나타나 개다래 분말 첨가량이 많아질수록 대조군에 비해 유의적으로 증가하는 경향을 보였다(p<0.001). 관능검사 결과, 삼킨 후 느낌, 외관, 향, 질감 맛과 전반적인 기호도에서 개다래 분말 3% 첨가군이 6.20으로 가장 높게 평가되어 개다래의 다양한 생리활성과 항산화 활성과 같은 기능성은 식품 소재로 활용가치가 있으며, 이를 활용한 머핀의 개발도 가능할 것으로 판단된다.

• 대한의생명과학회지
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• 제29권2호
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• pp.88-94
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• 2023

In oriental medicine, the fruit of Actinidia polygama has long been used to alleviate the symptoms of gout, arthritis, and inflammation. In this study, it was to designed to analyze the antibacterial activity of A. polygama ethanol extract (APEE) against Streptococcus mutans, one of the major strains for dental caries, and Porphyromonas gingivalis, one of the critical strains for periodontal disease. The antibacterial activity of APEE was analyzed by disk diffusion, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) assays. In addition, it was also analyzed the inhibitory effect of APEE on bacterial growth and biofilm formation against both oral pathogens. APEE exhibited its antibacterial effect through the inhibited bacterial diffusion as well as low concentration of MIC and MBC. In addition, APEE significantly inhibited not only bacterial growth but also biofilm formation in a dose-dependent manner. Consequently, APEE showed potent antibacterial activity against both S. mutans and P. gingivalis, which indicates that APEE might be used as a potential antibacterial material for the improvement of oral healthcare.

• 약학회지
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• 제47권5호
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• pp.307-310
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• 2003

Ethanol extract of Actinidia polygama Max. (AP) was examined for hypouricemic activity in potassium oxonateinduced hyperuricemic animal model. Ethanol extract of AP lowered the plasma uric acid level by 20％ when compared to the hyperuricemic control group. AP ether fraction at a dose of 100 mg/kg showed 27％ reduction, whose efficacy was comparable to the probenecid-treated group. AP ether fraction-treated group also showed 23％ reduction of urate in urine and this result suggests that AP ether fraction has anti-gout activity probably due to xanthine oxidase inhibition.

• 대한통합의학회지
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• 제11권2호
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• pp.119-128
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• 2023

Purpose : The fruit of Actinidia polygama has been used in oriental medicine for the treatment of gout, rheumatoid arthritis, and inflammation. Though A. polygama exhibited anti-inflammatory activity in RAW 264.7 cells and carrageenan-induced rat paw edema, the exact mechanism for anti-inflammation was not evaluated yet. In this study, the anti-inflammatory mechanisms of A. polygama ethanol extract (APEE) in lipopolysaccharide (LPS) stimulated RAW 264.7 cells. Methods : WST-1 assay was applied to analyze the cytotoxic effect of APEE in RAW 264.7 cells. The productions of nitric oxide (NO) and prostaglandin (PG) E₂ were analyzed by the Griess reaction and enzyme immunoassay (EIA) assay, respectively. In addition, protein expressions for inducible NO synthase (iNOS) and cyclooxygenase (COX)-2 were measured by Western blot analysis. The activated status of an inflammatory transcription factor, NF-κ B, and its upstream signaling molecules, mitogen-activated protein kinases (MAPKs), was also evaluated by Western blot analysis. Results : As a result, APEE treatment did not exhibit any cytotoxicity until the concentration of 200 ㎍/㎖. APEE treatment significantly inhibited NO and PGE₂ productions as well as their enzymes, iNOS and COX-2 in a dose-dependent manner. The inflammatory transcription factor, NF-κ B, was also attenuated by APEE treatment. In addition, the phosphorylated status of MAPKs such as extracellular regulated kinase (ERK), c-jun NH2 kinase (JNK), and p38, were significantly diminished by APEE treatment in LPS stimulated RAW 264.7 cells. Conclusion : Consequently, APEE treatment significantly attenuated the production of inflammatory mediators and their enzyme expressions in LPS-stimulated RAW 264.7 cells. The inflammatory transcription factor, NF-κ B, and upstream signaling molecules, MAPKs, were also significantly attenuated by APEE treatment in LPS-activated RAW 264.7 cells. These results indicate that APEE might be a candidate to be utilized as a promising candidate for the treatment of inflammatory disorders.

• 생약학회지
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• 제36권1호통권140호
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• pp.26-33
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• 2005

Korean folk medicine 'Da Rae Ip' has been used to cure intestinal catarrh, stomach cancer and acute gastritis. The botanical origin of the crude drug has never been studied pharmacognostically. To clarify the botanical origin of 'Da Rae Ip', the morphological and anatomical characteristics of the leaves of Actinidia species growing in Korea and Japan; i.e. A. arguta, A. arguta var. rufinervis, A. kolomikta, A. polygama, A. rufa were studied. As a result, it was clarified that 'Da Rae Ip' was the leaf of Actinidia arguta and Actinidia polygama.

• 생약학회지
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• 제48권2호
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• pp.108-112
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• 2017

To isolate and identify the constituents of the fruit galls of Actinidium polygama as well as evaluate the anti-inflammatory effects of the isolated compounds, a total of five triterpenes were isolated including $2{\alpha},3{\alpha},24$-trihydroxy-12-ursene-23-al-28-oic acid (1), corosolic acid (2), $2{\alpha},3{\alpha},24$-trihydroxyurs-12,20(30)-dien-28-oic acid (3), $2{\alpha},3{\alpha},24$-trihydroxyolean-12-en-28-oic acid (4), and $2{\alpha},3{\alpha},24$-trihydroxyurs-12-en-28-oic acid (pygenic acid B) (5). Compounds 1, 3, and 4 were isolated from the fruit galls of A. polygama for the first time. Compound 3 decreased NO production in LPS-stimulated RAW264.7 macrophages and mouse microglia BV2 cells in a concentration-dependent manner.

• Archives of Pharmacal Research
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• 제26권12호
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• pp.1061-1066
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• 2003

The fruit of Actinidia polygama (AP) has long been used as a folk medicine in Korea for treating pain, rheumatic arthritis and inflammation. The present investigation was carried out to determine the in vivo and in vitro anti-inflammatory activity of AP using several animal models of inflammation. The 70% ethanol extract of the fruit of AP significantly inhibited acetic acidinduced, vascular permeability in a dose dependent manner (23%, 38%, and 41 % inhibition at doses of 200 mg/kg, 500 mg/kg and 1000 mg/kg, respectively). This effect was maintained in AP water-soluble fraction (APW). The APW fraction also showed significant inhibitory activity against the rat paw edema induced by a single treatment of carrageenan. In vitro experiments were performed to demonstrate the inhibitory activities of APW (100 $\mu$ g/ml) on lipopolysaccharide (LPS)-induced nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$) production. The results showed that APW dose-dependently suppressed LPS-induced NO production in RAW 264.7 macrophages without a notable cytotoxic effect and also decreased inducible NO synthase (iNOS) protein expression. APW also showed a significant inhibitory effect in LPS-induced $PGE_2$ production and cyclooxygenase-2 (COX-2) expression.

• 한국유기농업학회지
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• 제19권spc호
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• pp.30-34
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• 2011

A. arguta is found nationwide whether the altitude is low or high if good drainage is achieved with plenty of water. The distribution of A. polygama seems to be more limited to higher altitude than A. arguta so it can be found in deep valley of highland even in Mt. Halla. A. kolomikta seems rather limited to colder area than A. arguta to Mt. Jiri to the south. Meanwhile, A. rufa is very confined to southern islands including Jeollanamdo and Jeju islands. Some institutes still continue to utilize Korean native Actinidia plants commercially since early 1980's. More collaborative efforts are needed for further development of these plants nationwide.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.262.2-263
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• 2002

The fruits of various Actinidia species are known to be used as a folk remedy for treatment of various inflammatory and analgesic ailments. Effects of the ethanol extracts and fractions from the fruits of Actinidia polygama (Sieb. et Zucc.) Maxim (Actinidiaceae) were studied using various in vivo and in vtro models of inflammation in mice and rats. (omitted)