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http://dx.doi.org/10.15188/kjopp.2022.08.36.4.120

Effect of Actinidia polygama on LPS-induced Inflammation in Mouse BV2 Microglial cells  

Kim, Kitae (Department of Internal Medicine of Clinical Korean Medicine, Semyung University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.36, no.4, 2022 , pp. 120-124 More about this Journal
Abstract
Actinidia polygama has long been used in traditional Korean medicine to treat rheumatoid arthritis and gout. Although numerous chemical compounds in the fruit extracts of A. polygama have been characterized and their role in inhibiting nitric oxide (NO) production has been reported, the anti-inflammatory properties of A. polygama extracts remain to be explored. In this study, we investigated the in-vivo effect of A. polygama extract on lipopolysaccharide (LPS)-induced inflammation in BV-2 microglial cell lines. We discovered that 100% ethyl alcohol extract of A. polygama effectively attenuates the release of NO and is superior to both water extract and 50% ethanol extract. Using MTT assay, western blot, and ELISA on LPS-induced BV-2 microglial cells lines, we established the ability of A. polygama extract to markedly suppress the expressions of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), and pro-inflammatory cytokines, such as tumor necrosis factor alpha and interleukin-6. These results reveal that the anti-inflammatory property of A. polygama in BV-2 microglial cells is due to the downregulation of iNOS, COX-2, MAPK protein, and pro-inflammatory cytokines.
Keywords
Actinidia polygama; BV2 microglial cells; Anti-inflammatory; MAPK; Cytokine;
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