Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effect of Actinidia polygama on LPS-induced Inflammation in Mouse BV2 Microglial cells

목천료자(木天蓼子)가 LPS로 유되된 Mouse BV2 Microglial cells의 염증반응에 미치는 영향

  • Kim, Kitae (Department of Internal Medicine of Clinical Korean Medicine, Semyung University)
  • 김기태 (세명대학교 부속 제천한방병원 내과학교실)
  • Received : 2022.01.22
  • Accepted : 2022.06.28
  • Published : 2022.08.25
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Abstract

Actinidia polygama has long been used in traditional Korean medicine to treat rheumatoid arthritis and gout. Although numerous chemical compounds in the fruit extracts of A. polygama have been characterized and their role in inhibiting nitric oxide (NO) production has been reported, the anti-inflammatory properties of A. polygama extracts remain to be explored. In this study, we investigated the in-vivo effect of A. polygama extract on lipopolysaccharide (LPS)-induced inflammation in BV-2 microglial cell lines. We discovered that 100% ethyl alcohol extract of A. polygama effectively attenuates the release of NO and is superior to both water extract and 50% ethanol extract. Using MTT assay, western blot, and ELISA on LPS-induced BV-2 microglial cells lines, we established the ability of A. polygama extract to markedly suppress the expressions of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), and pro-inflammatory cytokines, such as tumor necrosis factor alpha and interleukin-6. These results reveal that the anti-inflammatory property of A. polygama in BV-2 microglial cells is due to the downregulation of iNOS, COX-2, MAPK protein, and pro-inflammatory cytokines.

Keywords

Acknowledgement

이 논문은 2021학년도 세명대학교 교내 학술연구비 지원에 의해 수행된 연구임.

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