• Korean Journal of Plant Resources
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• v.17 no.2
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• pp.107-115
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• 2004

To develop universal primers for phylogenetic analysis and species-specific marker for breeding program of kiwifruit, eighteens primers were designed from kiwifruit genome-specific repeat sequences. Seven species including twenty two varieties collected from native eastern Asia were examined using 18 to 22 mer kiwifruit target(KT) primers. among eighteen primers, we selected seven primers for phylogenetic relationship. The genus Actinidia was divided into two large groups; group I,A. arguta, A. melanandra, A. kolomikta, and A. marcrosperma, characterized by the non-hair in fruits and loaves or a few pubescences only in young stage, which belongs to the section Leiocarpae, and group II, A. chinensis, A. deliciosa, and A. eriantha, characterized by a lot of hairs only in young fruit stage and with a lot of hairs or fuzzes in leaves and branches, which belongs to the section Stellatae. Group II especially belongs to the series Perfectae of the section Stellatae and was divided into two subgroups; subgroup I containing A. chinensis and A. deliciosa, and subgroup II containing A. eriantha. In contrast, the two species, A. chinensis and A. deliciosa, which are known to have common parents, were divided into two independent subgroups with 80％ of a similarity value. On the other hand, we selected KT6F for variety specific bands, KT12E primers for 'Hayward' and 'Tomuri'. KT7F or KT12F primers were useful for analysis of inheritance pattern in kiwifruit cross-breeding. We suggest that these primers will be a powerful tool for elucidating phylogenetic relationship and selection of novelty kiwifruit in a breeding program.

• Applied Microscopy
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• v.36 no.3
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• pp.195-208
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• 2006

Using light and scanning electron microscopy morphological studies of pollens were conducted to determine the sectional boundary and the relationships among eight species of section Zygophyllidium and its five related species of subg. Poinsettia. Pollen characters of 13 species were described, and their diagnostic keys were presented here. Based on the sculpturing patterns and the results of numerical analysis using 10 pollen characters 13 species were divided into four Pollen types: type I, having micro-reticulate shaped pollen (E. hexagona, E. lagunensis, E. hexagonoides and E. bilobata), type II, having reticulate shaped pollen (E. delicatula, E. extipulata, E. dentata, E. heterophylla, E. pulcherrima and E. cyathophora), type III, micro-reticulate shaped pollen with platten muri (E. lacera), and type IV, haying foveolate shaped pollen grains (E. chersonesa and E. eriantha). The results of pollen morphological studies suggest that the sect. Zygophyllidium should be delimited based on only four species, E. hexagona, E. lagunensis, E. hexagonoides and E. bilobata, E. delicatural and E. exstipulata of sect. Zygophyllidium must be transferred to subg. Poinsettia based on pollen morphology. E. eriantha and E. chersonesa having forveolate sculpturing pattern of pollen grains seem to be closely related with species from subg. Agaloma, and additional studies of these species are needed to determine the species relationships within Agaloma group.

• Horticultural Science & Technology
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• v.33 no.2
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• pp.259-267
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• 2015

This study was conducted to comparatively evaluate antioxidant capacity (AC) of seven cultivars of kiwifruit (Actinidia spp.) and their protective effects on neuronal PC-12 cells. The contents of total phenolics (TP) and total flavonoids (TF) of kiwifruits were also examined. Five cultivars of kiwifruit, Actinidia chinensis (cv. Haehyang and cv. Haegeum), A. eriantha (cv. Bidan), A. arguta ${\times}$ A. deliciosa (cv. Mansoo), and A. arguta (cv. Chiak), were bred in Korea, while two cultivars, A. deliciosa (cv. Hayward) and A. linguiensis (accession number 041AE), originated from New Zealand and China, respectively. Skin extracts of kiwifruit showed higher TP, TF, and AC than flesh extracts. The highest levels of TP and AC were found in cv. Bidan flesh extract among cultivars studied, but the TF content of cv. Bidan flesh extract was the lowest. The kiwifruit bred in Korea had higher AC than their counterparts. AC of kiwifruit had a highly positive linear correlation with TP and TF. The flesh extracts from cv. Hayward, cv. Haehyang, and cv. Haegeum significantly (p < 0.05) prevented PC-12 cells from oxidative stress induced using $H_2O_2$ compared to a control with $H_2O_2$ only. Overall, our results suggest that kiwifruit bred in Korea may offer a good source of antioxidants and serve as functional materials.

• Journal of Plant Biotechnology
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• v.44 no.3
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• pp.303-311
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• 2017

In this study, random amplified polymorphic DNA (RAPD) and sequence-related amplified polymorphism (SRAP) analyses were used for evaluation of genetic diversity of 61 kiwifruit (Actinidia spp.) germplasms including domestic and overseas collection cultivars. Forty RAPD primers were detected in a total of 230 polymorphic bands with an average of 5.75. Thirty-two SRAP primer combinations were detected in a total of 204 polymorphic bands with an average 6.38. By unweighted pair-group method arithmetic average cluster analysis using 434 polymorphic bands, kiwifruit germplasms were classified in three groups with similarity value of 0.680. Cluster I consisted of 46 kiwifruit germplasms belonging to A. deliciosa, A. chinensis, A. deliciosa ${\times}$ A. arguta, A. chinensis ${\times}$ A. arguta, and A. chinensis ${\times}$ A. deliciosa. Cluster II consisted of seven germplasms belonging to A. arguta and 'Skinny Green', a cultivar derived from a cross between A. arguta and A. deliciosa. Cluster III consisted of seven germplasms belonging to A. rufa, A. hemsleyana, A. macrosperma, A. polygama, and A. eriantha. Genetic similarity values among tested kiwifruit germplasms ranged from 0.479-0.991, and average similarity value was 0.717. Similarity value was highest (0.991) between NHK0038 (A. deliciosa) and NHK0040 (A. deliciosa), and lowest (0.479) between 'Hayward' (A. deliciosa) and K5-1-22 (A. arguta).

• Korean Journal of Plant Taxonomy
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• v.38 no.4
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• pp.389-403
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• 2008

Seed morphological studies of 12 species of sect. Zygophyllidium and related Poinsettia using light and scanning electron microscopy were conducted to test the hypotheses of the sectional boundaries and species relationships. Characteristics of seeds, and the keys of identification of 12 species were presented. Seeds of 12 species were divided into 6 types based on the UPGMA tree from numerical analyses using nine seed characters. Type I, including E. uniglandulosa, is characterized by the foveolate seed with granulate testa cells. Type II, including species E. exstipulata, E. lagunensis, E. bilobata, E. hexagonoides and E. chersonesa, was recognized by the presence of inter-cellular granules among testa cells. Type III, including E. cyathophora, has a seed with acute apex, and concentric circles on the surface of testa cells. Type IV, including species of E. dentata and E. pentadactyla, has a seed with caruncle and lacking inter-cellular granules among testa cells. Type V, including E. hexagona, was characterized by the ovate seeds and lacking inter-cellular granules. Type VI, including E. eriantha and E. lacera, was recognized by the seeds with rectangular shape with caruncle. Based on the seed morphology, sect. Zygophyllidium should be defined only species with E. exstipulata, E. lagunensis, E. bilobata, E. hexagonoides and E. chersonesa. E. hexagona, traditionally included in this section, was closely related to E. dentata from Poinsettia.

• Animal Bioscience
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• v.34 no.6
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• pp.1022-1028
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• 2021

Objective: Supplemental nitrate is known to be an effective tool to mitigate methane emission by ruminants. Based on theoretical considerations, supplemental nitrate can improve but also deteriorate the growth performance. The overall effect of supplemental nitrate on growth performance, however, is not yet known. The objective of the current study was therefore to evaluate the effect of a higher dose of NO3- on overall growth performance when feeding either Pangola grass hay or rice straw. Methods: Thirty-two crossbred, 3-month-old Thai native×Anglo-Nubian crossbred male goats were used. The experiment had a 2×2 factorial design with an experimental period of 60 days. Eight goats were randomly allocated to each dietary treatment, i.e. a ration containing either Pangola hay (Digitaria eriantha Steud) or rice straw (Oryza Sativa) as a source of roughage, supplemented with a concentrate containing either 3.2% or 4.8% potassium nitrate. The rations were formulated to be isonitrogenous. The animals were weighed at the start of the experiment and at days 30 and 60. Feces were collected during the last five days of each 30-day period. Results: High-nitrate increased overall DM intake by approximately 3%, irrespective the source of roughage, but only the goats fed a rice straw-based ration responded with an increase in body weight (BW). Thus, the overall feed conversion ratio (kg feed/kg BW gain) was influenced by roughage source ×nitrate and decreased by almost 60% when the goats were fed rice straw in combination with a high versus a low dietary nitrate content. The digestibility of macronutrients was only affected by the source of roughage and the digestibility of organic matter, crude protein, and neutral detergent fibre was greater when the goats were fed Pangola hay. Conclusion: It was concluded that the replacement of soybean meal by nitrate improves the growth performance of meat goats fed rations based on rice straw, but not Pangola hay.