Browse > Article
http://dx.doi.org/10.5010/JPB.2017.44.3.303

Genetic diversity in kiwifruit germplasm evaluated using RAPD and SRAP markers  

Cho, Kang Hee (Fruit Research Division, National Institute of Horticultural and Herbal Science, RDA)
Kwack, Yong-Bum (Namhae Branch, National Institute of Horticultural and Herbal Science, RDA)
Park, Seo Jun (Fruit Research Division, National Institute of Horticultural and Herbal Science, RDA)
Kim, Se Hee (Fruit Research Division, National Institute of Horticultural and Herbal Science, RDA)
Lee, Han Chan (Fruit Research Division, National Institute of Horticultural and Herbal Science, RDA)
Kim, Mi Young (Fruit Research Division, National Institute of Horticultural and Herbal Science, RDA)
Publication Information
Journal of Plant Biotechnology / v.44, no.3, 2017 , pp. 303-311 More about this Journal
Abstract
In this study, random amplified polymorphic DNA (RAPD) and sequence-related amplified polymorphism (SRAP) analyses were used for evaluation of genetic diversity of 61 kiwifruit (Actinidia spp.) germplasms including domestic and overseas collection cultivars. Forty RAPD primers were detected in a total of 230 polymorphic bands with an average of 5.75. Thirty-two SRAP primer combinations were detected in a total of 204 polymorphic bands with an average 6.38. By unweighted pair-group method arithmetic average cluster analysis using 434 polymorphic bands, kiwifruit germplasms were classified in three groups with similarity value of 0.680. Cluster I consisted of 46 kiwifruit germplasms belonging to A. deliciosa, A. chinensis, A. deliciosa ${\times}$ A. arguta, A. chinensis ${\times}$ A. arguta, and A. chinensis ${\times}$ A. deliciosa. Cluster II consisted of seven germplasms belonging to A. arguta and 'Skinny Green', a cultivar derived from a cross between A. arguta and A. deliciosa. Cluster III consisted of seven germplasms belonging to A. rufa, A. hemsleyana, A. macrosperma, A. polygama, and A. eriantha. Genetic similarity values among tested kiwifruit germplasms ranged from 0.479-0.991, and average similarity value was 0.717. Similarity value was highest (0.991) between NHK0038 (A. deliciosa) and NHK0040 (A. deliciosa), and lowest (0.479) between 'Hayward' (A. deliciosa) and K5-1-22 (A. arguta).
Keywords
Actidinia; Genetic diversity; Polymorphism; RAPD; SRAP;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Guo D, Zhang J, Liu C, Zhang G, Li M, Zhang Q (2012) Genetic variability and relationships between and within grape cultivated varieties and wild species based on SRAP markers. Tree Genet Genom 8:789-800   DOI
2 Huang HW, Li Z, Li J (2002) Phylogenetic relationships in Actinidia as revealed by RAPD analysis. J Amer Soc Hort Sci 127:759-766
3 Jaeger SR, Harker FR (2005) Consumer evaluation of novel kiwifruit: willingness-to-pay. J Sci Food Agric 85:2519-2526   DOI
4 Kabaluk JT, Kempler C, Toivonen PMA (1997) Actinidia argutacharacteristics relevant to commercialization production. Fruit Var J 51:117-122
5 Korkovelos AE, Mavromatis AG, Huang WG, Hagidimitriou M, Giakoundis A, Goulas CK (2008) Effectiveness of SSR molecular markers in evaluating the phylogenetic relationships among eight Actinidia species. Sci Hortic 116:305-310   DOI
6 Li G, Quiros CF (2001) Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theor Appl Genet 103:455-461   DOI
7 Liu Y, Liu Y, Huang H (2010) Genetic variation and natural hybridization among sympatric Actinidia species and the implications for introgression breeding of kiwifruit. Tree Genet Genomes 6:801-813   DOI
8 Nishiyama I, Oota T (2007). Fruits of the Actinidia genus. Adv Food Nutr Res 52:293-324
9 Palombi MA, Damiano C (2002) Comparison between RAPD and SSR molecular markers in detecting genetic variation in kiwifruit (Actinidia deliciosa A. Chev). Plant Cell Rep 20:1061-1066   DOI
10 Novo M, Rome S, Rey M, Prado MJ, Gonzalez (2010) Identification and sequence characterisation of molecular markers polymorphic between male kiwifruit (Actinidia chinensis var. deliciosa (A. Chev.) A. Chev.) accessions exhibiting different flowering time. Euphytica 175:109-121   DOI
11 Park YK, Han Si, Kim SH, Kang MS (2011) Changes of photosynthesis, leaf and fruit characteristics of Actinidia arguta and hybrid kiwi (A. arguta ${\times}$ A. deliciosa) according to crown layer. J Korean For Soc 100:8-13
12 Prado MJ, Gonzalez MV, Romo S, Herrera MY (2007) Adventitious plant regeneration on leaf explants from adult male kiwifruit and AFLP analysis of genetic variation. Plant Cell Tiss Cult 88:1-10   DOI
13 Stanica F, Zuccherelli G (2007) New selections of Actinidia arguta from the Romanian breeding program. Acta Hortic. 753:263-267
14 Uzun A, Gulsen O, Seday U, Bircan M, Yilmaz KU (2010) SRAP based genetic analysis of some apricot cultivars. Romanian Biotechnol Lett 15:5396-5404
15 Williams MH, Boyd LM, McNeilage MA, MacRae EA, Ferguson RA, Martin PJ (2003) Development and commercialization of 'Baby Kiwi' (Actinidia argute Planch). Acta Hortic 610:81-86
16 Cho KH, Heo S, Kim JH, Shin IS, Kim SH, Kim DH, Han SE, Kim HR (2010) Analysis of genetic diversity of apple cultivars using RAPD and SSR markers. Kor J Breed Sci 42:525-533
17 Zhao Y, Lin H, Guo Y, Liu Z, Guo X, Li K (2013) Genetic linkage maps of pear based on SRAP markers. Pak J Bot 45:1265-1271
18 Zhen Y, Li Z, Huang H (2004) Molecular characterization of kiwifruit (Actinidia) cultivars and selections using SSR markers. J Amer Soc Hort Sci 129:374-382
19 Ahmad R, Potter D, Southwick SM (2004) Genotyping of peach and nectarine cultivars with SSR and SRAP molecular markers. J Am Soc Hort Sci 129:204-210
20 Budak H, Shearman RC, Parmaksiz I, Dweikat I (2004) Comparative analysis of seeded and vegetative biotype buffalograsses based on phylogenetic relationship using ISSRs, SSRs, RAPDs, and SRAPs. Theor Appl Genet 109:280-288
21 Cipriani G, Bella RD, Testolin R (1996) Screening RAPD primers for molecular taxonomy and cultivar fingerprinting in the genus Actinidia. Euphytica 90:169-174   DOI
22 Ferguson AR, Huang HW (2007) Genetic resources of kiwifruit: domestication and breeding. Hor Rev 33:1-121
23 Crowhurst RN, Lints R, Atkinson RG, Gardner RC (1990) Restriction fragment length polymorphisms in the genus Actinidia (Actinidiaceae). Plant Syst Evol 172:193-203   DOI
24 Ellsworth DL, Rittenhous KD, Honeycutt RL (1993) Artifactual variation in randomly amplified polymorphic DNA banding patterns. BioTechniques 14:214-217
25 Ferguson AR (2007) The need for characterisation and evaluation of germplasms: kiwifruit as an example. Euphytica 154: 371-382   DOI
26 Ferguson AR, Seal AG, McNeilage MA, Fraser LG, Harvey CF, Beatson RA (1996) Kiwifruit, p. 371-417. In: J Janick, JN Moore (eds.). Fruit breeding, Vol. II: Vine and small fruit crops. Prentice Hall, New York