Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Genetic diversity in kiwifruit germplasm evaluated using RAPD and SRAP markers

RAPD와 SRAP 마커를 이용한 참다래 유전자원의 유전적 다양성

  • Cho, Kang Hee (Fruit Research Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kwack, Yong-Bum (Namhae Branch, National Institute of Horticultural and Herbal Science, RDA) ;
  • Park, Seo Jun (Fruit Research Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kim, Se Hee (Fruit Research Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Lee, Han Chan (Fruit Research Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kim, Mi Young (Fruit Research Division, National Institute of Horticultural and Herbal Science, RDA)
  • 조강희 (농촌진흥청 국립원예특작과학원 과수과) ;
  • 곽용범 (농촌진흥청 국립원예특작과학원 남해출장소) ;
  • 박서준 (농촌진흥청 국립원예특작과학원 과수과) ;
  • 김세희 (농촌진흥청 국립원예특작과학원 과수과) ;
  • 이한찬 (농촌진흥청 국립원예특작과학원 과수과) ;
  • 김미영 (농촌진흥청 국립원예특작과학원 과수과)
  • Received : 2017.05.30
  • Accepted : 2017.07.31
  • Published : 2017.09.30
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Abstract

In this study, random amplified polymorphic DNA (RAPD) and sequence-related amplified polymorphism (SRAP) analyses were used for evaluation of genetic diversity of 61 kiwifruit (Actinidia spp.) germplasms including domestic and overseas collection cultivars. Forty RAPD primers were detected in a total of 230 polymorphic bands with an average of 5.75. Thirty-two SRAP primer combinations were detected in a total of 204 polymorphic bands with an average 6.38. By unweighted pair-group method arithmetic average cluster analysis using 434 polymorphic bands, kiwifruit germplasms were classified in three groups with similarity value of 0.680. Cluster I consisted of 46 kiwifruit germplasms belonging to A. deliciosa, A. chinensis, A. deliciosa ${\times}$ A. arguta, A. chinensis ${\times}$ A. arguta, and A. chinensis ${\times}$ A. deliciosa. Cluster II consisted of seven germplasms belonging to A. arguta and 'Skinny Green', a cultivar derived from a cross between A. arguta and A. deliciosa. Cluster III consisted of seven germplasms belonging to A. rufa, A. hemsleyana, A. macrosperma, A. polygama, and A. eriantha. Genetic similarity values among tested kiwifruit germplasms ranged from 0.479-0.991, and average similarity value was 0.717. Similarity value was highest (0.991) between NHK0038 (A. deliciosa) and NHK0040 (A. deliciosa), and lowest (0.479) between 'Hayward' (A. deliciosa) and K5-1-22 (A. arguta).

본 연구는 참다래(Actinidia spp.) 유전자원의 유전적 다양성을 평가하기 위하여 재배품종 및 국내 외에서 수집한 참다래 61점을 대상으로 RAPD와 SRAP 분석을 수행하였다. RAPD 분석에서 40종의 선발 primer를 이용하여 230개의 다형성 밴드를 얻었으며, 평균 다형성 밴드 수는 5.75개였다. 32종의 primer 조합을 이용한 SRAP 분석에서 204개의 다형성 밴드를 획득하였고, 평균 다형성 밴드 수는 6.38 개였다. RAPD와 SRAP 분석에서 획득된 434개의 다형성 밴드를 이용하여 비가중 평균결합 방식으로 집괴분석한 결과 유전적 유사도 지수 0.680을 기준으로 3개의 그룹으로 분류되었다. 제1그룹에는 A. deliciosa와 A. chinensis에 속하는 품종과 A. deliciosa ${\times}$ A. arguta, A. chinensis ${\times}$ A. arguta, A. chinensis ${\times}$ A. deliciosa의 교잡종에 속하는 46점의 유전자원이 포함되었다. 제2그룹에는 A. arguta에 속하는 유전자원 7점과 A. arguta ${\times}$ A. deliciosa의 교잡종인 '스키니그린'이 포함되었다. 제3그룹에는 A. rufa, A. hemsleyana, A. macrosperma, A. polygama, A. eriantha 종에 속하는 유전자원 7점이 포함되었다. 참다래 유전자원 간 유전적 유사도 값은 0.479~0.991의 범위로 평균 유전적 유사도는 0.717이었다. 가장 높은 유사도 값(0.991)을 나타낸 유전자원은 NHK0038(A. deliciosa)과 NHK0040(A. deliciosa) 간이었고 가장 낮은 유사도 값(0.479)을 나타낸 유전자원은 '헤이워드'(A. deliciosa)와 K5-1-22(A. arguta) 간이었다.

Keywords

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