• Journal of Plant Biotechnology
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• v.44 no.1
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• pp.89-96
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• 2017

The CRISPR/Cas9 is a core technology that can result in a paradigm for breeding new varieties. This study describes in detail the sgRNA design, vector construction, and the development of a transgenic plant and its molecular analysis, and demonstrates how gene editing technology through the CRISPR/Cas9 system can be applied easily and accurately. CRISPR/Cas9 facilitates targeted gene editing through RNA-guided DNA cleavage, followed by cellular DNA repair mechanisms that introduce sequence changes at the site of cleavage. It also allows the generation of heritable-targeted gene mutations and corrections. Here, we present detailed procedures involved in the CRISPR/Cas9 system to acquire faster, easier and more cost-efficient gene edited transgenic rice. The protocol described here establishes the strategies and steps for the selection of targets, design of sgRNA, vector construction, and analysis of the transgenic lines. The same principles can be used to customize the versatile CRISPR/Cas9 system, for application to other plant species.

• Journal of Korean Academy of Nursing Administration
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• v.6 no.1
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• pp.83-96
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• 2000

With the increased use of computers in medical fields, we can consider introducing computer mediated communication into the area of patient care such as medical or health counseling and education. But little was known about what problems can be counseled, what is the main direction of counseling, and what difficulties are met during the computer mediated counseling. So I conducted this study. This is a fundamental study concerning the health counseling by the inter-net. In this study, medical counseling using a method of questioning -answering through a computer mediated communication system was carried out from June 1998 to May 1999. I analyzed the contents of questions and answers by the ICPC classification. The ICPC classification emerged as a standard for information categorization in Primary Health Care within a few years. One of the most prominent features of this classification is the inclusion of the complaints of the patients (symptoms & complaints) and the social problems (Chapter Z). Thus, and for the first time, the demand of patients may be assessed such as it is expressed in the reality. The ICPC list is now an international standard whose validity is not questioned any more. It is translated in more than twenty different languages. The data collected were analyzed by descriptive statistical method, c2 test by cross tabulations. Followings are the results of this study. 1. The 3,054 users composed of 64% of males and 36% of females. The highest number of users was showed in the age group of $25{\sim}29$ (39.4%). 2. During the one-year period, the average number of counseling per user was 2.5 cases and per day was 21.1 cases. 3. In classifying symptoms and complaints by 17 chapter, digestive(17.6%), general and unspecified (12.7%), skin(12.7%) and musculoskeletal system (9.6%) were most common questions. 4. The distribution of diagnosis by 17 chapter, general and unspecified (21.4%), digestive (15.3%), skin (11.9%) and mus- culoskeletal system (8.9%) were most common answers. 5. Many users wanted to know symptoms and complaints(3,609 cases), treatment principles (2,928 cases), prognosis and preventive methods for health problems which were previously diagnosed (284 cases).

• Archives of Craniofacial Surgery
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• v.10 no.2
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• pp.91-96
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• 2009

Purpose: Frontal sinus fractures are relatively less common than other facial bone fractures. They are commonly concomitant with other facial bone fractures. They can cause severe complications but the optimal treatment of frontal sinus fractures remains controversial. Currently, many principles of treatment were introduced variously. The authors present valid and simplified protocols of treatment for frontal sinus fractures based on fracture pattern, nasofrontal duct injury, and complications. Methods: A retrospective chart review was performed on 36 cases of frontal sinus fractures between January, 2004 and January, 2009. The average age of patients was 33.7 years. Fracture patterns were classified by displacement of anterior and posterior wall, comminution, nasofrontal duct injury. These fractures were classified in 4 groups: I. anterior wall linear fractures; II. anterior wall displaced fractures; III. anterior wall displaced and posterior wall linear fractures; IV. anterior wall and posterior wall displaced fractures. Also, assessment of nasofrontal duct injury was conducted with preoperative coronal section computed tomographic scan and intraoperative findings. Patients were treated with various procedures including open reduction and internal fixation, obliteration, galeal frontalis flap and cranialization. Results: 12 patients are group I (33.3 percent), 14 patient were group II (38.8 percent), group III, IV were 5 each (13.9 percent). Frontal sinus fractures were commonly associated with zygomatic fractures (21.8 percent). 9 patients had nasofrontal duct injury. The complication rate was 25 percent (9 patients), including hypoesthesia, slight forehead irregularity, transient cerebrospinal fluid leakage. Conclusion: The critical element of successful frontal sinus fracture repair is precise diagnosis of the fracture pattern and nasofrontal duct injury. The main goal of management is the restoration of the sinus function and aesthetic preservation.

• Journal of Pharmacopuncture
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• v.9 no.1
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• pp.33-44
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• 2006

Objectives : We performed this study in order to investigate the effects of Sedang-hwan(世糖丸) on the diabetes mellitus. Methods : We injected a vein with 65mg/kg of streptozotocin(STZ) on the rats. And then administered Sedang-hwan; 18.7mg/kg/day to sample groups and observed the weight and glucose level, insulin, C-peptide, triglyceride, albumin, T-protein, BUN(Blood Urea Nitrogen) and creatinine in blood. Results : 1. The sample groups showed a high suppressive effect of weight loss(P=0.002). 2. The sample groups' glucose level showed a effective in lowering level(P=0.001). 3. The sample groups showed a higher insulin level than control groups. 4. The sample groups showed a higher C-peptide level than control groups(P=0.001). 5. The sample groups' triglyceride level showed a effective in lowering level. 6. The sample groups showed a higher HDL-cholesterol level than control groups(P<0.001). 7. The sample groups showed a higher T-protein level than control groups(P=0.04). 8. The sample groups showed a higher albumin level than control groups(P<0.001). 9. The sample groups' creatinine level showed a effective in lowering level(P=0.04). 10. The sample groups' BUN level showed a effective in lowering level(P=0.03). Conclusions : Conclusively, Sedang-hwan was recognized to have decrease effect of serum glucose, triglyceride, creatinine, BUN of the diabetic rats induced by streptozotocin. It was suggested that Sedang-hwan can be used for effective diabetes mellitus medical treatment. It is also required to study further about detailed principles of blood-sugar dropping effect by Sedang-hwan, and recovery by diabetes mellitus.

• Natural Product Sciences
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• v.18 no.1
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• pp.47-51
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• 2012

Among 38 Vietnamese medicinal plant extracts investigated for their ${\alpha}$-glucosidase inhibitory activity, 35 extracts showed $IC_{50}$ values below $250{\mu}g/mL$. The MeOH extracts of the heartwood of Oroxylum indicum, the seeds of Caesalpinia sappan, and the fruits of Xanthium strumarium exhibited strong ${\alpha}$-glucosidase inhibitory activity with $IC_{50}$ values less than $50{\mu}g/mL$. Fractionation of the MeOH extract of the heartwood of O. indicum led to the isolation of oroxylin A (1), oroxyloside (2), hispidulin (3), apigenin (4), ficusal (5), balanophonin (6), 2- (1-hydroxymethylethyl)-4H,9H-naphtho[2,3-b]furan-4,9-dione (7), salicylic acid (8), p-hydroxybenzoic acid (9), protocatechuic acid (10), isovanillin (11), and ${\beta}$-hydroxypropiovanillon (12). Compounds 1 - 3, 5, 6, 8, 10, and 12 showed more potent activities, with $IC_{50}$ values ranging from 2.13 to $133.51{\mu}M$, than a positive control acabose ($IC_{50}$, $241.85{\mu}M$). The kinetic study indicated that oroxyloside (2) displayed mixed-type inhibition with inhibition constant (Ki) was $3.56{\mu}M$.

• Korean Journal of Medicinal Crop Science
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• v.16 no.3
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• pp.173-182
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• 2008

Prunus sargentii of Rosaceae familiy, has been reported to have radical scavenging activity and anti-inflammatory effect. On these facts, this research was conducted to evaluate pharmaceutical activities of the bark extracts P. sargentii. Free radical scavenging activities of fraction (Fr) -5${\sim}$10 isolated from P. sargentii was higher than 80% respectively at 10ppm. The superoxide dismutase (SOD)-like activity of Fr-5, 9 were about 97, 84%, respectively at 1,000 ppm. Xanthine oxidase inhibitory effect of Fr-9, 10 were about 75, 78%, respectively at 1,000 ppm. The tyrosinase inhibitory effect related to skin-whitening was 72, 68% in Fr-2, 9 isolated from Prunus sargentii R. at 1,000 ppm. Hyaluronidase inhibition activity related to the anti-inflammation effect was 98% for Fr-8 at 500 ppm. Isolation of the methanol soluble fraction from P. sargentii yielded two major phenol compounds, (-)-epicatechin and taxifolin. The structure of the compound was certainly determined by chemical analyses, as well as NMR and Mass spectroscopy. The present study was carried out in a search for new cosmetic material from the bark from P. sargentii. and, (-)-epicatechin and taxifolin were isolated as active principles. So P. sargentii R. methanolic extracts may be used for the cosmetic material.

• The Transactions of the Korea Information Processing Society
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• v.4 no.9
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• pp.2391-2402
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• 1997

TINA is the Open Networking Architecture which as newly introduced software architecture shares the network managements and the service managements on the Distributed Processing Environment. In this paper, based on the concepts and principles defined up to now, we implement and test the CM(Connection Management) Package included in CM which is one of the six functional areas into which TINA Management Architecture is devided. Before the implementation we learn the basic concepts accepted by the four architectures in TINA, and medelling concept of the CM. The interfaces of eaxh information objects explain the functions of computational objects implemented, and the message flows among the computational objects show the connection setup and release procedures.

• Economic and Environmental Geology
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• v.9 no.4
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• pp.213-223
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• 1976

Proceeding from general elementary principles to more specific abstract problems, we have attempted the rearrangement of the research results as they are known at present concerning the propagation and attennation of the elastic wave in submarine layers. We have derived the elementary equations of the elastic wave. In addition, the relationship of the propagation of the elastic waves in the sea water mass and the reflection of the waves from the water-sediment interface are treated and presented in different sections.

• 한국신재생에너지학회:학술대회논문집
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• 2010.06a
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• pp.236.1-236.1
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• 2010

Due to global warming, the need to secure an alternative resource has become more important nationally. Due to the high tidal range of up to 9.7m on the west coast of Korea, numerous tidal current projects are being planned and constructed. The rotor, which initially converts the energy, is a very important component because it affects the efficiency of the entire system, and its performance is determined by various design variables. In this paper, a design guideline of current generating HAT rotor and acceptable field rotor in offshore environment is proposed. To design HAT rotor model, wind mill rotor design principles and turbine theories were applied based on a field HAT rotor experimental data. To verify the compatibility of the rotor design method and to analyze the properties of design factors, 3D CFD model was designed and analysed by ANSYS CFX. The analysis results and findings are summarized in the paper.

• Journal of Satellite, Information and Communications
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• v.9 no.3
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• pp.59-63
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• 2014

This paper describes the basic principles of 3D broadcast system and proposes new 3D broadcast technology that reduces the amount of data by applying CS(Compressed Sensing). Differences between Sampling theory and the CS technology concept were described. Recently proposed CS algorithm AMP(Approximate Message Passing) and CoSaMP(Compressive Sampling Matched Pursuit) were described. This paper compared an accuracy between two algorithms and a calculation time that image data compressed and restored by these algorithms. As result determines a low complexity algorithm for 3D broadcast system.