• Asian-Australasian Journal of Animal Sciences
• /
• v.18 no.5
• /
• pp.734-740
• /
• 2005

The blood samples were collected from dairy cows at the same milking stage. The single-strand conformation polymorphism (PCR-SSCP) method was used to analyze for polymorphism at the 5'flanking region of the hsp70 gene. The mRNA expression levels of HSP70, HSF1, Bcl-2 and Bax-$\alpha$ at different daily-mean-temperature were analyzed by relative quantitative RTPCR. The DNA content, cell phase and the ratio of apoptosis of lymphocytes in peripheral blood of dairy cattle at different daily-meantemperature were determined by FCM. The PCR-SSCP products of primer pair 1 showed polymorphisms and could be divided into four genotypes: aa, ab, ac, cc, with the cis-acting element (CCAAT box) included. Mutations in the hsp70 5'flanking region (468-752 bp) had different effects on mRNA expression of HSP70, HSF1, Bcl-2 and Bax-$\alpha$. The ac genotypic cows showed higher expressions of HSP70mRNA, HSF1mRNA and Bcl-2mRNA/Bax-$\alpha$mRNA and lower ratio of apoptosis. These mutation sites can be used as molecular genetic markers to assist selection for anti-heat stress cows.

• Korean Journal of Ecology and Environment
• /
• v.49 no.1
• /
• pp.22-30
• /
• 2016

Environmental changes exert harmful effects on organisms inhabiting coastal regions. These changes are also associated with reduced production in aquaculture farms. In this study, we investigated internal and external responses of two Bivalvia species (Crassostrea gigas and Mytilus galloprovincialis) in Gamak Bay under stressful environmental conditions in aquaculture farms. We investigated external responses such as weight, size, and environment exposure time, and analyzed the expression of the HSP70 gene. C. gigas HSP70 gene expression level was significantly high in the C3 aquaculture farm site, but the weight and size of C. gigas were high in the C2 aquaculture farm site. The response of C. gigas HSP70 mRNA was associated with the environmental exposure time in each aquaculture farm. Expression of M. galloprovincialis HSP70 gene was found to be significantly higher in the M2 aquaculture farm site than in the M1 site, whereas the weight of M. galloprovincialis was observed to be higher in the M1 site. The size and environmental exposure time of M. galloprovincialis were similar between M1 and M2 sites. In addition, HSP70 sequences of C. gigas and M. galloprovincialis showed high similarity with that of another marine species. According to our results, there were differences in internal responses following environmental stress in aquaculture farms, with respect to HSP70 gene expression. The results suggest that the HSP70 gene is a useful molecular indicator for monitoring stress responses in Bivalvia species in the field.

• Trans-
• /
• v.7
• /
• pp.19-48
• /
• 2019

This study was described to examine the meaning of the media exhibition work <70mK>, which aims to capture the topography of the collective consciousness of the Korean people through large-scale interviews. <70mK> edits and organizes interview images of individual beings in mosaic-like layouts and forms, creating video exhibitions and holding exhibitions. The objects in the split frame show the continuity of differences that reveal their own thoughts and personalities. This is a synchronic and conscious collective typology in which the intrinsic nature of the individuals is embodied in a simultaneous and holistic image. Interview images reveal their own form as a actual being and convey the intrinsic nature of one's own as oral information. <70mK> constructs a new individualization by aesthetically structuring the forms and information of life individuals in the extension of a specific group. The beings in the frame are not communicating with each other and are looking straight ahead. it conveys to visitors their relationship and personality as the preindividual reality. It is the repetitive arrangement and composition of heterogeneity and difference that each individual shows, and is a chain operation that includes collective identity behind it. <70mK> constructs the direct images and sounds of individual interviewee, creating a new form of information transfer called Video Art Exhibition. This makes metaphors and perceptions of the meaning and process of transindividual relationships and the meaning of psychic individuation and collective individuation. This is an appropriate case to explain with modern technology and individualization of Gilbert Simondon thought together with the meaning of becoming and relation of individualization. The exhibition space constructed by <70mK> is an aesthetic methodology of the psychic and collective meaning and its relationship to a particular group of individuals through which they are connected. Simondon studied the meaning of the process of individualization and the meaning of becoming, and is a philosopher who positively considered the potential of modern technology. <70mK> is a new individual as structured and generated ethical reality mediated by modern technology mechanisms and network behaviors. It is an case of an aesthetic and practical methodology of how interviews function as 'transduction' in the process of individualization in which technology is cooperated. The direct images and sounds of <70mK> are systems in which the information of life individuals is carried, amplified, accumulated and transmitted. It is also a new individual as a psychic and collective landscape. It is a newly became exhibition art work through the multiple individualization, and is a representation of transindividual meanings and process. The media exhibition art of individualized metastable states leads to new relationships in which viewers perceive the same preindividual reality and feel affectivity. The exhibition space of <70mK> becomes a stage for preparing the actual possibility of the transindividual group beyond the representation of the semantic function.

• Journal of the Korean Society of Fisheries and Ocean Technology
• /
• v.31 no.3
• /
• pp.228-239
• /
• 1995

A model experiment on the pair midwater trawl net which is prevailing in Denmark is carried out to get the basic data available for Korean pair bottom trawlers. The model net was made in 1/30 scale considering the Tauti's Similarity law of fishing gear and the dimension of experimental tank. The vertical opening, horizontal opening, towing tension and net working depth of the model net were determined in the tank within the towing velocity 0.46~1.15m/sec, front weight 15.5~62.0g and distance between paired boats 5~8m(which correpond to 2~5k't in towing velocity, 70~280kg in weight and 150~240m in distance respectively in the prototype net). The results got from the model experiment can be converted into the full scale net as follows; 1. Vertical opening showed the largest value of 32m at the condition of 2k't in towing velocity, 280kg in front weight and 150m in the distance between paired boats, and the smallest value of 6m at the condition of 5k't in towing velocity, 70kg in front weight and 240m in the distance between paired boats. 2. Horizontal opening showed the largest value of 45m at the condition of 5k't in towing velocity, 70kg in front weight and 240m in the distance between paired boats, and the smallest value of 33m at the condition of 2k't in towing velocity, 280kg in front weight and 150m in the distance between paired boats. 3. Towing tension showed the largest value of 10, 000kg at the condition of 5k't in towing velocity, 280kg in front weight and 240m in the distance between paired boats, and the smallest value of 1, 600kg at the condition of 2k't in towing velocity, 70kg in front weight and 150m in the distance between paired boats. 4. Net working depth showed the largest value of 38m at the condition of 2k't in towing velocity, 280kg in front weight and 150m in the distance between paired boats, and the smallest value of 6m at the condition of 5k't in towing velocity, 70kg in front weight and 240m in the distance between paired boats. 5. Net opening area showed the largest value of 1, 100m super(2) at the condition of 2k't in towing velocity, 280kg in front weight and 180m in the distance between paired boats, and the smallest value of 250m super(2) at the condition of 5k't in towing velocity, 70kg in front weight and 240m in the distance between paired boats.

• Journal of Animal Environmental Science
• /
• v.19 no.1
• /
• pp.19-24
• /
• 2013

This study is to promote domestic environmentally appropriately conditions for the range 60~70 m, the development of the small and medium-sized sprayer ability measurement that can be used as a general-purpose paddy fields, orchards, and not consolidated areas. The sprayer designed and manufactured that can spraying effectively distance to within 60~70 m. Distribution of sprayed pesticides were obtained and analyzed by measuring the weight. Performance analysed using the buckets were placed horizontally 12.5 m, 70 m vertical intervals by $5m{\times}2.5m$, so total 68 buckets were placed. Total amounts of sprayed quantity was $2,000{\ell}$ and maximum spray distance was about 70 m. Reasonably sprayed region was 5 m~10 m vertically symmetrical. Showed a lot of spraying water in 5 m~40 m distance, and in 45~60 m tended to spraying water decreased slightly. Maximum spraying distance was 70 m.

• Elastomers and Composites
• /
• v.40 no.3
• /
• pp.174-180
• /
• 2005

Sonochemical synthesis of fullerene oxides $[C_{70}O_n](n=1{\sim}2)$ by fullerene$[C_{70}]$ and metal hexacarbonyl complexes $M(CO)_6$(M=Cr, Mo, W) took place under air atmosphere. The reactivity of fullerene$[C_{70}]$ and several metal hexacarbonyl complexes $M(CO)_6$(M=Cr, Mo, W) under same ultrasonic condition increased in the order of $Mo(CO)_6$ > $W(CO)_6$ > $Cr(CO)_6$. The MALDI-TOF-MS, UV-visible spectra, and HPLC analysis confirmed that the products of sonochemical reaction were $[C_{70}O_n](n=1{\sim}2)$.

• Journal of Aquaculture
• /
• v.19 no.4
• /
• pp.315-322
• /
• 2006

The objective of the present study was to investigate the expression of heat shock protein 90 (HSP90) and 70 (HSP70) mRNA as cellular stress responses, the levels of plasma cortisol with glucose as neuro-endocrine stress responses during water temperature rising in freshwater adapted black porgy, Acanthopagrus schlegeli. A cDNA fragment of 891 (HSP90) and 465 (HSP70) bp was cloned from black porgy testis by Reverse transcription-polymerase chain reaction (RT-PCR) with primers designed from the conserved regions of other teleost. The PCR product of HSP90 showed very high homology to red seabream (99%), rainbow trout (95%), Atlantic salmon (94%), zebrafish (94%) HSP90, HSP70 of black porgy was also highly similar to those of rainbow trout (96%), silver seabream (95%), zebrafish (95%) HSP70. Water temperature rising ($20{\sim}30^{\circ}C$) induced elevation of HSP90 mRNA in black porgy gonad, liver, brain, intestine and kidney, whereas it resulted in an induction of the HSP70 mRNA expression in gonad only. Plasma cortisol levels increased significantly at $30^{\circ}C$ in the fish compared to those at $20^{\circ}C$. Glucose levels of the fish showed a tendency of co-increase with cortisol during water temperature rising. These results suggest that increased HSP90 mRNA in liver with plasma cortisol following heat shock may be related to increasing glucose for homeostasis in this species.

• Journal of environmental and Sanitary engineering
• /
• v.12 no.2
• /
• pp.59-64
• /
• 1997

For purification of a 70kDa toxic protein of mosquitocidal delta-endotoxin from B. thuringiensis strain H9B, immuno-affinity chromatography was performed. After separation of 70kDa toxic proteins from the delta-endotoxin of the strain H9B on SDS-PAGE, the 70kDa toxic protein was subcutaneously injected into rabbit for making a polyclonal antibody. A anti-70kDa toxic protein was purified by a column chromatography packed with protein A-sepharose 4B gels. The 70kDa toxic protein from delta-endotoxin of the strain H9B was also purified by an immuno-affinity chromatography packed with CNBr-activated sepharose 4B gels conjugated anti-70kDa toxic protein after elution with 1/10M citric acid-1/5M Na$_{2}$HPO$_{4}$ buffer(pH3.2) containing 0.5M NaCl. The 70kDa toxic protein was purified through only one step-separation system, was demonstrated by SDS-PAGE and immunoblot.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.14 no.10
• /
• pp.5091-5096
• /
• 2013

Houttuynia cordata Thunb is a famous traditional Chinese medicine. Also it has rich in functional and bioactive substances with outstanding immunopotentiation, anti-cancer, antimicrobial, and anti-aging effects. This study was evaluated water and 70% ethanol extracts of Houttuynia cordata Thunb using in vitro system antioxidant properties methods. The scavenging DPPH and ABTS radicals of 2 mg/mL 70% ethanol extract were 91.8 and 54.8%, respectively. Also, The FRAP assay of this showed increase in activity. In results, the antioxidant properties of 70% ethanol extract increased significantly compared to water extract(p < 0.05). Therefore, 70% ethanol extract of Houttuynia cordata Thunb may contribute to antioxidant property.

• Asian-Australasian Journal of Animal Sciences
• /
• v.21 no.8
• /
• pp.1116-1126
• /
• 2008

The objective of this study was to investigate the expression and localization of heat shock protein 70 (Hsp70) and its mRNA in the heart, liver, and kidney of acutely heat-stressed broilers at various stressing times. Male AA broilers (n = 100) were randomly divided into 5 groups of 20 birds per group. After 30 d of adaptive feeding at ambient temperature, 80 experimental broilers were suddenly heat stressed by increasing the environmental temperature from $22{\pm}1^{\circ}C$ to $37{\pm}1^{\circ}C$. The 4 groups were heat stressed for 2, 3, 5, and 10 h, respectively. The localizations of Hsp70 protein and mRNA, determined by immunohistochemical staining and in situ hybridization, respectively, were demonstrated to be tissue dependent, implying that different tissues have differential sensibilities to heat stress. Intense Hsp70 staining was identified in the vascular endothelial cell of heart, liver and kidney, suggesting an association between expression of Hsp70 in vascular endothelial cell and functional recovery of blood vessels after heat shock treatment. Ante-mortem heat stress had a significant effect on the expression of Hsp70 protein and mRNA. The quantitation of Hsp70 protein and mRNA were both time and tissue dependent. During the exposure to heat stress, the heart, liver and kidney of broiler chickens exhibited increased amounts of Hsp70 protein and mRNA. The expression of hsp70 mRNA in the heart, liver and kidney of heat-stressed broilers increased significantly and attained the highest level after a 2-h exposure to elevated temperatures. However, significant elevations in Hsp70 protein occurred after 2, 5, and 3 h of heat stressing, respectively, indicating that the stress-induced responses vary among different tissues.