Browse > Article
http://dx.doi.org/10.5713/ajas.2008.70703

Effect of Acute Heat Stress on Heat Shock Protein 70 and Its Corresponding mRNA Expression in the Heart, Liver, and Kidney of Broilers  

Yu, Jimian (The Key Laboratory of Animal Pathology, College of Veterinary Medicine Nanjing Agricultural University)
Bao, Endong (The Key Laboratory of Animal Pathology, College of Veterinary Medicine Nanjing Agricultural University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.21, no.8, 2008 , pp. 1116-1126 More about this Journal
Abstract
The objective of this study was to investigate the expression and localization of heat shock protein 70 (Hsp70) and its mRNA in the heart, liver, and kidney of acutely heat-stressed broilers at various stressing times. Male AA broilers (n = 100) were randomly divided into 5 groups of 20 birds per group. After 30 d of adaptive feeding at ambient temperature, 80 experimental broilers were suddenly heat stressed by increasing the environmental temperature from $22{\pm}1^{\circ}C$ to $37{\pm}1^{\circ}C$. The 4 groups were heat stressed for 2, 3, 5, and 10 h, respectively. The localizations of Hsp70 protein and mRNA, determined by immunohistochemical staining and in situ hybridization, respectively, were demonstrated to be tissue dependent, implying that different tissues have differential sensibilities to heat stress. Intense Hsp70 staining was identified in the vascular endothelial cell of heart, liver and kidney, suggesting an association between expression of Hsp70 in vascular endothelial cell and functional recovery of blood vessels after heat shock treatment. Ante-mortem heat stress had a significant effect on the expression of Hsp70 protein and mRNA. The quantitation of Hsp70 protein and mRNA were both time and tissue dependent. During the exposure to heat stress, the heart, liver and kidney of broiler chickens exhibited increased amounts of Hsp70 protein and mRNA. The expression of hsp70 mRNA in the heart, liver and kidney of heat-stressed broilers increased significantly and attained the highest level after a 2-h exposure to elevated temperatures. However, significant elevations in Hsp70 protein occurred after 2, 5, and 3 h of heat stressing, respectively, indicating that the stress-induced responses vary among different tissues.
Keywords
Heat Stress; Heat Shock Protein 70; Localization; Quantitation; Broilers;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By Web Of Science : 3  (Related Records In Web of Science)
Times Cited By SCOPUS : 5
연도 인용수 순위
1 Bryantsev, A. L., S. A. Loktionova, O. P. Ilyinskaya, E. M. Tararak, H. H. Kampinga and A. E. Kabakov. 2002. Distribution, phosphorylation, and activities of Hsp25 in heat-stressed H9c2 myoblasts: a functional link to cytoprotection. Cell Stress Chaperones 7:146-155.   DOI   ScienceOn
2 Bukau, B., E. Deuerling, C. Pfund and E. A. Craig. 2000. Getting Newly Synthesized Proteins into Shape. Cell 101(2):119-122.   DOI   ScienceOn
3 Amrani, M., N. Latif, K. Morrison, C. C. Gray, J. Gray, J. Jayakumar, A. Corbett, M. Goodwin, Dunn and M. Yacoub. 1998. Relative induction of heat shock protein in coronary endothelial cells and cardiomyocytes: implications for myocardial protection. J. Thorac. Cardiovasc. Surg. 115:200-209.   DOI   ScienceOn
4 Arrigo, A. P. 2000. HSPs as novel regulators of programmed cell death and tumorigenicity. Path. Bio. 48:280-288.
5 Levy, E., Y. Hasin, G. Navon and M. Horowitz. 1997. Chronic heat improves mechanical and metabolic response of trained rat heart on ischemia and reperfusion. Am. J. Physiol. Heart Circ. Physiol. 272:H2085-H2094.   DOI
6 Lin, W. Q., R. A. Vandyke, H. M. Marsh and J. R. Trudell. 1994. Nuclear translocation of heat shock protein 72 in liver cells of halothane-exposed rats. Biochem. Biophys. Res. Commun. 199:647-652.   DOI   ScienceOn
7 Mahmoud, K. Z., M. M. Beck, S. E. Scheideler, K. P. Anderson and S. D. Kachman. 1996. Acute high environmental temperature and calcium-estrogen relationships in hen. Poult. Sci. 75:1555-1562.   DOI   ScienceOn
8 Heydari, A. R., B. Wu, R. Takahashi, R. Strong and A. Richardson. 1993. Expression of heat shock protein 70 is altered by age and diet at the level of transcription. Mol. Cell. Biol. 13:2909-2918.   DOI
9 Chee, K. M., M. K. Chung, J. H. Choi and Y. K. Chung. 2005. Effects of dietary vitamin C and E on egg shell quality of broiler breeder hens exposed to heat stress. Asian-Aust. J. Anim. Sci. 18:545-551.   과학기술학회마을   DOI
10 Craig, E. A. 1985. The heat shock response. CRC. Crit. Rev. Biochem. 16:135-140.
11 Craig, E. A. and C. A. Gross. 1991. Is hsp70 the cellular thermometer? Trends Biochem. Sci. 16:35-40.
12 Dechesne, C. J., H. N. Kim, T. S. Nowak and R. J. Wenthold. 1992. Expression of heat shock protein, HSP72, in the guinea pig and rat cochlea after hyperthermia; immunochemical and in situ hybridization analysis. Hear Res. 59:195-204.   DOI   ScienceOn
13 Edens, F. W., C. H. Hill and S. Wang. 1992. Heat shock protein response in phosphorus-deficient heat-stressed broiler chickens. Comp. Biochem. Physiol. B 103:827-831.   DOI   ScienceOn
14 Gabriel, J. E., J. A. Ferro, R. M. P. Stefani, M. I. T. Ferro, S. L. Gomes and M. Macari. 1996. Effect of acute heat stress on heat shock protein 70 messenger RNA and on heat shock protein expression in the liver of broilers. Br. Poult. Sci. 37:443-449.   DOI   ScienceOn
15 Geraert, P. A., S. Guillaumin and B. Leclercq. 1993. Are genetically lean broilers more resistant to hot climate? Br. Poult. Sci. 34(4):643-653.   DOI   ScienceOn
16 Baqchi, M., M. Ireland, M. Katar and H. Maisel. 2001. Heat shock proteins of chicken lens. J. Cell. Biochem. 82(3):409-414.   DOI   ScienceOn
17 Hartl, F. U. 1996. Molecular chaperones in cellular protein folding. Nature 381:571-580.   DOI   ScienceOn
18 Geraert, P. A., J. C. Padilha and S. Guillaumin. 1996. Metabolic and endocrine changes induced by chronic heat exposure in broiler chickens: growth performance, body composition and energy retention. Br. J. Nutr. 75(2):195-204.   DOI   ScienceOn
19 Glover, J. R. and S. Lindquist. 1998. Hsp104, Hsp70, and Hsp40: a novel chaperone system that rescues previously aggregated proteins. Cell 94(1):73-82.   DOI   ScienceOn
20 Guerrierojr, V. and D. A. Raynes. 1990. Synthesis of heat stress protein in lymphocytes from livestock. J. Anim. Sci. 68:2779-2783.   DOI
21 Hartl, F. U. and M. Hayer-Hartl. 2002. Molecular chaperones in the cytosol: from nascent chain to folded protein. Sci. 295(5561):1852-1858.   DOI   ScienceOn
22 Zhang, S. L., M. Guo, X. S. Zhang, W. X. Zhang, X. Y. Li and C. S. Xu. 2000. Study on the expression of HSP70 induced by heat shock in mouse hepatocytes. Chinese J. Anat. 123:4.
23 Morimoto, R. I. 1998. Regulation of the heat shock transcriptional response: cross talk between a family of heat shock factors, molecular chaperones, and negative regulators. Genes Dev. 12:3788-3796.   DOI   ScienceOn
24 Van Laack, R. L. J. M., C. Faustman and J. G. Sebranek. 1993. Pork quality and the expression of stress protein Hsp70 in swine. J. Anim. Sci. 71:2958-2964.   DOI
25 Yahav, S., A. Shamay, G. Horev, D. Bar-ilan, O. Genina and M. Friedman-einat. 1997. Effect of acquisition of improved thermotolerance on the induction of heat shock proteins in broiler chickens. Poult. Sci. 76:1428-1434.   DOI
26 White, F. P. 1980. The synthesis and possible transport of specific proteins by cells associated with brain capillaries. J. Neurochem. 35(1):88-94.   DOI
27 Wu, C. 1995. Heat shock transcription factors: structure and regulation. Annu. Rev. Cell. Biol. 11:441-469.   DOI   ScienceOn
28 Hightower, L. E. 1991. Heat shock, stress proteins, chaperones and proteotoxicity. Cell 66:191-197.   DOI   ScienceOn
29 Gutierrez, J. A. and V. Guerrierojr. 1991. Quantitation of Hsp70 in tissues using a competitive enzyme-linked immunosorbent assay. J. Immunol. Meth. 143:81-88.   DOI   ScienceOn
30 Hickey, E., S. E. Brandon, R. Potter, G. Stein, J. Stein and L. A. Weber. 1986. Sequence and organization of genes encoding the human 27 kDa heat shock protein. Nucleic Acids Res. 14:4127-4145.   DOI   ScienceOn
31 Ipek, A., O. Canbolat and A. Karabulut. 2007. The effect of vitamin E and vitamin C on the performance of Japanese Quails (Coturnix Coturnix Japonica) reared under heat stress during growth and egg production period. Asian-Aust. J. Anim. Sci. 20:252-256.   과학기술학회마을
32 Jakubowicz-Gil, J., B. Pawlikowska-Pawlega, T. Piersiak, J. Pawelec and A. Gawron. 2005. Quercetin suppresses heat shock-induced nuclear translocation of Hsp72. Folia Histochemi. Cytobiol. 43:123-128.
33 Lepore, D. A., J. V. Hurley, A. G. Stewart, W. A. Morrison and R. L. Anderson. 2000. Prior heat stress improves survival of ischemic-reperfused skeletal muscle in vivo. Muscle Nerve 23: 1847-1855.   DOI   ScienceOn
34 Tao, X., Z. Y. Zhang, H. Dong, H. Zhang and H. Xin. 2006. Responses of thyroid hormones of market-size broilers to thermoneutral constant and warm cyclic temperatures. Poult. Sci. 85:1520-1528.
35 van der Hel, W., M. W. Versteqen, L. Pijls and M. van Kampen. 1992. Effect of two-day temperature exposure of neonatal broiler chicks on growth performance and body composition during two weeks at normal conditions. Poult. Sci. 71(12):2014-2021.   DOI   ScienceOn
36 Zulkifli, I., Nwe New Htin, A. R. Alimon, T. C. Loh and M. Hair-Bejo. 2007. Dietary selection of fat by heat-stressed broiler chickens. Asian-Aust. J. Anim. Sci. 20:245-251.   과학기술학회마을
37 Velazquez, J. M. and S. Lindquist. 1984. hsp70: Nuclear concentration during environmental stress and cytoplasmic storage during recovery. Cell 36:655-662.   DOI   ScienceOn
38 Wang, J. H., H. P. Redmond, R. W. Watson, C. Condron and D. Bouchier-Hayes. 1995. Induction of heat shock protein 72 prevents neutrophil-mediated human endothelial cell necrosis. Arch. Surg. 130(12):1260-1265.   DOI   ScienceOn
39 Zietkiewicz, S., J. Krzewska and K. Liberek. 2004. Successive and synergistic action of the Hsp70 and Hsp100 chaperones in protein disaggregation. J. Biol. Chem. 279:44376-44383.   DOI   ScienceOn
40 Zulkifli, I., S. A. Mysahra and L. Z. Jin. 2004. Dietary supplementation of Betaine (Betafin) and response to high temperature stress in male broiler chickens. Asian-Aust. J. Anim. Sci. 17:244-249.   과학기술학회마을   DOI
41 Wang, T. T., A. S. Chiang, J. J. Chu, T. J. Cheng, T. M. Chen and Y. K. Lai. 1998. Concomitant alterations in distribution of 70 kDa heat shock proteins, cytoskeleton and organelles in heat shocked 9L cells. Int. J. Biochem. Cell. Biol. 30:745-759.   DOI   ScienceOn
42 Shabtay, A. and Z. Arad. 2005. Ectothermy and endothermy: evolutionary perspectives of thermoprotection by HSPs. J. Exp. Biol. 208:2773-2781.   DOI   ScienceOn
43 Shabtay, A. and Z. Arad. 2006. Reciprocal activation of HSF1 and HSF3 in brain and blood tissues: is redundancy developmentally related? Am. J. Physiol. Regul. Integr. Comp. Physiol. 291:R566-R572.   DOI
44 Tankson, J. D., Y. Vizzier-Thaxton, J. P. Thaxton, J. D. May and J. A. Cameron. 2001. Stress and nutritional quality of broilers. Poult. Sci. 80:1384-1389.   DOI
45 Shaila, S., S. Angshuman, K. Abhijeet, M. Samindranath, Pal and K. Jayanta. 2005. Flufenoxuron, an acylurea insect growth regulator, alters development of Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) by modulating levels of chitin, soluble protein content, and HSP70 and p34cdc2 in the larval tissues. Pestic. Biochem. Physiol. 85:84-90.   DOI   ScienceOn
46 Shim, K. S., K. T. Hwang, M. W. Son and G. H. Park. 2006. Lipid metabolism and peroxidation in broiler chickens under chronic heat stress. Asian-Aust. J. Anim. Sci. 19:1206-1211.   DOI
47 Sun, P. M., Y. T. Liu, Y. G. Zhao, E. D. Bao and Z. L. Wang. 2007. Relationship between heat damages and HSPs mRNA in persistent heat stressed broilers. Agric. Sci. China 6:227-233.   DOI   ScienceOn
48 Wang, S. and F. W. Edens. 1993. Stress-induced heat-shock protein synthesis in peripheral leukocytes of turkeys, Meleagris Gallopavo. Comp. Biochem. Physiol. 106B:621-628.
49 Wang, S. and F. W. Edens. 1998. Heat conditioning induces heat shock proteins in broiler chickens and turkey poults. Poult. Sci. 77:1636-1645.   DOI
50 Wang, S. C., J. Chen, Y. Huang, X. F. Li and D. J. Zhang. 2007. Effect of heat stress on production performance and blood biochemical indices in broiler. China Poult. 15:11-13.
51 McCully, J. D., T. Myrmel, M. Lotz, I. B. Krukenkamp and S. Levitsky. 1995. The rapid expression of myocardial hsp70 mRNA and the heat shock 70 kDa protein can be achieved after only a brief period of retrograde hyperthermic perfusion. J. Mol. Cell. Cardiol. 27:873-882.   DOI   ScienceOn
52 Mariethoz, E., M. R. Jacquier-Sarlin, G. Multhoff, A. M. Healy, F. Tacchini-Cottier and B. S. Polla. 1997. Heat shock and proinflammatory stressors induce differential localization of heat shock proteins in human monocytes. Inflammation 21(6):629-642.   DOI   ScienceOn
53 Martin, F., J. M. Requena, J. Martin, C. Alonso and M. C. Lopez. 1993. Cytoplasmic-Nuclear translocation of the HSP70 protein during environmental stress in Trypanosoma cruzi. Biochem. Biophys. Res. Commun. 196:1155-1162.   DOI   ScienceOn
54 Mashaly, M. M., G. L. Hendricks, M. A. Kalama, A. E. Gehad, A. O. Abbas and P. H. Patterson. 2004. Effect of heat stress on production parameters and immune responses of commercial laying hens. Poult. Sci. 83(6):889-894.   DOI
55 Mayer, M. P. and B. Bukau. 2004. Hsp70 chaperones: Cellular functions and molecular mechanism. Cell. Mol. Life Sci. 62:670-684.   DOI   ScienceOn
56 Maloyan, A., A. Palmon and M. Horwitz. 1999. Heat acclimation increase the basal HSP72 level and alters its production dynamics during heat stress. Am. J. Physiol. Regul. Integr. Comp. Physiol. 276:R1505-R1515.
57 Marder, J. and Z. Arad. 1983. Acid-base regulation during thermal panting in the fowl (Gallus domesticus): Comparison between breeds. Comp. Biochem. Physiol. A 74:125-130.   DOI   ScienceOn
58 Marder, J. and Z. Arad. 1989. Panting and acid-base regulation in heat stressed birds. Comp. Biochem. Physiol. A 94:395-400.   DOI   ScienceOn
59 Sandercock, D. A., R. R. Hunter, G. R. Nute, M. A. Mitchell and P. M. Hocking. 2001. Acute heat stress-induced alterations in blood acid-base status and skeletal muscle membrane integrity in broiler chickens at two ages: implications for meat quality. Poult. Sci. 80:418-425.   DOI
60 Miron, T., K. Vancompernolle, J. Vandekerckhove, M. Wilchek and B. Geiger. 1991. A 25-kD inhibitor of actin polymerization: Is a low molecular mass heat shock protein. J. Cell. Biol. 114:255-261.   DOI   ScienceOn
61 Morimoto, R. I. 1993. Cells in stress: transcriptional activation of heat shock genes. Sci. 259:1409-1410.   DOI
62 Northcutt, J. K., E. A. Foegeding and F. W. Edens. 1994. Waterholding properties of thermally preconditioned chicken breast and leg meat. Poult. Sci. 73:308-316.   DOI   ScienceOn
63 Michael, L., Goodson and K. D. Sarge. 1995. Regulated expression of heat shock factor 1 isoforms with distinct leucine zipper arrays via tissue-dependent alternative splicing. Biochem. Biophys. Res. Commun. 211:943-949.   DOI   ScienceOn
64 Ogura, Y., Y. Naito, T. Tsurukawa, N. Ichinoseki-Sekine, N. Saga, T. Sugiura and S. Katamoto. 2007. Microwave hyperthermia treatment increases heat shock proteins in human skeletal muscle. Br. J. Sports Med. 41:453-455.   DOI   ScienceOn
65 McCormick, P. H., G. Chen, S. Tierney, C. J. Kelly and D. J. Bouchier-Hayes. 2003a. Clinically applicable thermal preconditioning attenuates leukocyte-endothelial interactions. J. Am. Coll. Surg. 197:71-78.   DOI   ScienceOn
66 McCormick, P. H., G. Chen, S. Tlerney, C. J. Kelly and D. J. Bouchier-Hayes. 2003b. Clinically relevant thermal preconditioning attenuates ischemia-reperfusion injury. J. Surg. Res. 109:24-30.   DOI   ScienceOn
67 Mahmoud, K. Z., F. W. Edens, E. J. Eisen and G. B. Havenstein. 2004. The effect of dietary phosphorus on heat shock protein mRNAs during acute heat stress in male broiler chickens (Gallus gallus). Comp. Biochem. Physiol. C 137:11-18.   DOI   ScienceOn
68 Knowlton, A. A. 1995. The role of heat shock proteins in the heart. J. Mol. Cell. Cardiol. 27:121-131.   DOI   ScienceOn
69 King, Y. T., C. S. Lin, J. H. Lin and W. C. Lee. 2002. Whole-body hyperthermia-induced thermotolerance is associated with the induction of Heat Shock Protein 70 in mice. J. Exp. Biol. 205:273-278.
70 Knowlton, A. A. 1994. Heat-shock proteins, stress and the heart. Ann. NY. Acad. Sci. 723:128-137.   DOI
71 Lavoie, J. N., H. Lambert, E. Hickey, L. A. Weber and J. Landry. 1995. Modulation of cellular thermoresistance and actin filament stability accompanies phosphorylation-induced changes in the oligomeric structure of heat shock protein 27. Mol. Cell. Biol. 15:505-516.   DOI
72 Park, H., I. Y. Ahn and H. E. Lee. 2007. Expression of heat shock protein 70 in the thermally stressed antarctic clam Laternula elliptica. Cell Stress Chaperones 12(3):275-282.   DOI   ScienceOn
73 Pivovarova, A. V., V. V. Mikhailova, I. S. Chernik, N. A. Chebotareva, D. I. Levitsky and N. B. Gusev. 2005. Effects of small heat shock proteins on the thermal denaturation and aggregation of F-actin. Biochem. Biophys. Res. Commun. 331:1548-1553.   DOI   ScienceOn
74 Pratt, W. B. 1993. The role of heat shock proteins in regulating the function, folding, and trafficking of the glucocorticoid receptor. J. Biol. Chem. 268:21455-21458.
75 Ryan, M. T. and N. Pfanner. 2001. Hsp70 proteins in protein translocation. Adv. Protein Chem. 59:223-242.   DOI
76 Belay, T. and R. G. Teeter. 1996. Effects of ambient temperature on broiler mineral balance partitioned into urinary and faecal loss. Br. Poult. Sci. 37:423-433.   DOI   ScienceOn