• Journal of Veterinary Science
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• v.22 no.6
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• pp.67.1-67.7
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• 2021

Background: Chlamydophila felis, formerly known as Chlamydia psittaci var. felis, is frequently associated with ocular, respiratory, and occasionally reproduction tract infections. Even though the infection is sometimes asymptomatic, it potentially results in a latent immunosuppressive infection. Objective: This study aimed to identify occurrences of feline chlamydophilosis, rarely reported in cats in Indonesia. Methods: The observation was conducted in three cats with clinical signs of Cp. felis infection, particularly relapsing conjunctivitis. The cats' histories were recorded based on owners' information. Conjunctival swabs were sampled for cytology examination and molecular assay detection. A phylogenetic tree was generated using MEGA-X software to reveal group clustering. A post-mortem examination was performed on the cat that died during an examination. Results: Cp. felis was detected in both cytological examination and polymerase chain reaction assay. The phylogenetic tree demonstrated that the Cp. felis isolated in this study clustered with several other isolates from the other countries. Cp. felis can be isolated from cats with different clinical manifestations and levels of severity. The chronic fatal infection demonstrated interstitial broncho-pneumonia under histopathological examination. Conclusions: Molecular assay of Cp. felis is always recommended to obtain a definitive diagnosis of feline chlamydophilosis since the disease can have various clinical manifestations. Even though it may be subclinical and is often not fatal, an infected cat may be a carrier that could spread the pathogen in the surrounding environment. Serious disease management is suggested to avoid high costs associated with regularly relapsing disease.

• Journal of Veterinary Science
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• v.22 no.1
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• pp.12.1-12.11
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• 2021

Background: Bats have been considered natural reservoirs for several pathogenic human coronaviruses (CoVs) in the last two decades. Recently, a bat CoV was detected in the Republic of Korea; its entire genome was sequenced and reported to be genetically similar to that of the severe acute respiratory syndrome CoV (SARS-CoV). Objectives: The objective of this study was to compare the genetic sequences of SARS-CoV, SARS-CoV-2, and the two Korean bat CoV strains 16BO133 and B15-21, to estimate the likelihood of an interaction between the Korean bat CoVs and the human angiotensin-converting enzyme 2 (ACE2) receptor. Methods: The phylogenetic analysis was conducted with the maximum-likelihood (ML) method using MEGA 7 software. The Korean bat CoVs receptor binding domain (RBD) of the spike protein was analyzed by comparative homology modeling using the SWISS-MODEL server. The binding energies of the complexes were calculated using PRODIGY and MM/GBGA. Results: Phylogenetic analyses of the entire RNA-dependent RNA polymerase, spike regions, and the complete genome revealed that the Korean CoVs, along with SARS-CoV and SARS-CoV-2, belong to the subgenus Sarbecovirus, within BetaCoVs. However, the two Korean CoVs were distinct from SARS-CoV-2. Specifically, the spike gene of the Korean CoVs, which is involved in host infection, differed from that of SARS-CoV-2, showing only 66.8%-67.0% nucleotide homology and presented deletions within the RBD, particularly within regions critical for cross-species transmission and that mediate interaction with ACE2. Binding free energy calculation revealed that the binding affinity of Korean bat CoV RBD to hACE2 was drastically lower than that of SARS-CoV and SARS-CoV-2. Conclusions: These results suggest that Korean bat CoVs are unlikely to bind to the human ACE2 receptor.

• Advances in nano research
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• v.13 no.4
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• pp.325-339
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• 2022

Endophytes ascertain a symbiotic relationship with plants as promoters of growth, defense mechanism etc. This study is a first report to screen the endophytic population in Waltheria indica, a tropical medicinal plant. 5 bacterial and 3 fungal strains in leaves, 3 bacterial and 1 yeast species in stems were differentiated morphologically and identified by biochemical and molecular methods. The phylogenetic tree of the isolated endophytes was constructed using MEGA X. Silver nanoparticles were biosynthesized from a rare endophytic bacterium Cupriavidus metallidurans isolated from the leaf of W. indica. The formation of silver nanoparticles was confirmed by UV-Visible spectrophotometer that evidenced a strong absorption band at 408.5 nm of UV-Visible range with crystalline nature and average particle size of 16.4 nm by Particle size analyzer. The Fourier Transform Infra-Red spectrum displayed the presence of various functional groups that stabilized the nanoparticles. X-ray diffraction peaks were conferred to face centered cubic structure. Transmission Electron Microscope and Scanning Electron Microscope revealed the spherical-shaped, polycrystalline nature with the presence of elemental silver analyzed by Energy Dispersive of X-Ray spectrum. Selected area electron diffraction also confirmed the orientation of AgNPs at 111, 200, 220, 311 planes similar to X-ray diffraction analysis. The synthesized nanoparticles are evaluated for antimicrobial activity against 7 bacterial and 3 fungal pathogens. A good zone of inhibition was observed against pathogenic bacteria than fungal pathogens. Thus the study could hold a key aspect in drug discovery research and other pharmacological conducts of human clinical conditions.

• Journal of Dental Anesthesia and Pain Medicine
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• v.23 no.2
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• pp.91-99
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• 2023

Background: Extrusion of debris is a major factor that results in postoperative pain during root canal treatment with various instruments and instrumentation techniques. Therefore, instrumentation techniques that extrude minimal debris into the periapical area while reducing pain are desirable. This study aimed to compare the incidence of postoperative pain and intake of analgesic medication (frequency and quantity) after endodontic treatment of mandibular posterior teeth using two single files and full-sequence continuous rotary systems with different kinematic motions. Methods: Thirty-five of 105 patients were assigned equally to three groups according to the instrumentation system used: ProTaper Next (PN) X2, 25/06 (Dentsply, Maillefer, Ballaigues, Switzerland), One Shape (OS), #0.25/06 (Micro Mega, Besancon, France), and Wave One Gold (WG), Red - #0.25, 0.07 (Dentsply, Maillefer, Ballaigues, Switzerland). Five specialists were included in this study design; each professional prepared 21 teeth, and randomly selected 7 per instrument system. The VAS sheet ranging from 0 to 10 was used to record the initial and postoperative pains at 24, 48, and 72 h, and 7^th day after single visit endodontic treatment in mandibular premolars and molars with a diagnosis of asymptomatic irreversible pulpitis with or without apical periodontitis. Postoperatively, an analgesic, ibuprofen 400 mg was administered for intolerable pain at a dose of 1 tablet for 6 h. The patients were asked over the telephone regarding postoperative pain at intervals of 24, 48, and 72 h, and 7^th day using a visual analogue scale. Result: There were no statistically significant differences among the PN, OS, and WG systems (P > 0.05) with regard to the incidence of postoperative pain at any of the four time points assessed. Conclusion: The intensity of postoperative pain, frequency, and analgesic intake were similar across all three types of instrument systems; however, the reciprocating single file (WG) was associated with less postoperative pain than the full sequence continuous rotary file.

• Journal of the Economic Geographical Society of Korea
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• v.18 no.3
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• pp.281-295
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• 2015

The purpose of this study is to analyze the residents' perceptions and assessments of impacts of the EXPO 2012 Yeosu Korea, hosted from May 15 to August 15, 2012. Residents of Yeosu area, in general, assessed the impacts of the EXPO highly positively and showed as a high degree of satisfaction. They evaluated the urban development effects of the EXPO as a 3.61 point in a 5 point scale and a total degree of satisfaction of 65.2%. In terms of perception and satisfaction index, it showed that 79.4% of an improvement of transportation infrastructure, 72.1% of an urban environmental improvement, 70.4% of an increase in visiting tourists, 69.0% of a raising of self-esteem as Yeosu citizens, 67.8% of an improvement of city's image, 66.2% of an enhancement of inner city transportation infrastructure, 60.5% of an enhancement of infrastructure of tourism, 51.3% of an local economy vitaliation, and 49.7% of a globalization effect in order. In conclusion, through the hosting of the EXPO 2012 Yeosu Korea, residents of Yeosu area assessed, the urban development in transportation infrastructure was moved advanced by eleven years and the residential environment improvement by eight years.

• KIPS Transactions on Software and Data Engineering
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• v.7 no.3
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• pp.99-106
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• 2018

In the process of acquiring information through the cyber ISR (Intelligence Surveillance Reconnaissance) and research into the agent to help decision-making, periodic communication between the C&C (Command and Control) server and the agent may not be possible. In this case, we have studied how to effectively surveillance and reconnaissance. Due to the network configuration, agents planted on infiltrated computers can not communicate seamlessly with C&C servers. In this case, the agent continues to collect data continuously, and in order to analyze the collected data within a short time in When communication is possible with the C&C server, it can utilize limited resources and time to continue its mission without being discovered. This research shows the superiority of incremental learning method over batch method through experiments. At an experiment with the restricted memory of 500 mega bytes, incremental learning method shows 10 times decrease in learning time. But at an experiment with the reuse of incorrectly classified data, the required time for relearn takes twice more.

• Journal of Korea Water Resources Association
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• v.49 no.4
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• pp.283-291
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• 2016

As the infrastructures and populations are the condensed in the mega city, urban flood management becomes very important due to the severe loss of lives and properties. For the more accurate calculation of runoff from the urban catchment, hourly or even minute rainfall data have been utilized. However, the time steps of the measured or forecasted data under climate change scenarios are longer than hourly, which causes the difficulty on the application. In this study, daily rainfall data was disaggregated into hourly using the stochastic method. Based on the historical hourly precipitation data, Gram Schmidt orthonormalization process and K-Nearest Neighbor Resampling (KNNR) method were applied to disaggregate daily precipitation into hourly. This method was originally developed to disaggregate yearly runoff data into monthly. Precipitation data has smaller probability density than runoff data, therefore, rainfall patterns considering the previous and next days were proposed as 7 different types. Disaggregated rainfall was resampled from the only same rainfall patterns to improve applicability. The proposed method was applied rainfall data observed at Seoul weather station where has 52 years hourly rainfall data and the disaggregated hourly data were compared to the measured data. The proposed method might be applied to disaggregate the climate change scenarios.

• Journal of Korean Tunnelling and Underground Space Association
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• v.18 no.5
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• pp.413-418
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• 2016

Recently, a mega project such as Korea-China or Korea-Japan undersea tunnel project has been emerged for detail discussion and the interest in undersea tunnel is on the rise. More severe damage by train fire is expected in undersea tunnel comparing to ground tunnel and thus the study on more efficient fire extinguishing system, besides existing disaster prevention design is underway. To that end, a full-scale fire tests using CAF fire extinguishing system which has been developed by modifying traditional foam fire extinguishing system for fire suppression at rescue station in timely manner were conducted over 7 times. The test was conducted after setting the rescue station in virtual tunnel with a car of KTX. As a result of using compressed air foam directly to the fire source, 30 liter of Heptane combustibles was extinguished within 1 minutes. Applicability of compressed air foam to train fire at rescue station in undersea tunnel was has been proven and further study is considered required while changing the nozzle angle and location so as to achieve quick and easy extinguishing goal, making use of the advantage of CAF, as well as to reduce the fire water and chemicals required.

• Journal of Life Science
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• v.30 no.7
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• pp.625-629
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• 2020

The Ocean is a rich source of diverse living organisms include viruses. In this study, we examined the microbial communities in the sea adjacent to Jeju Island in two seasons by metatranscriptomics. We collected and extracted total RNA, and, using the next-generation sequencing HiSeq 2000 and de novo transcriptome assembly, we identified 652,984 and 163,759 transcripts from the March and December samples, respectively. The most abundant organisms in March were bacteria, while eukaryotes were dominant in the December sample. The bacterial communities differed between the two samples, suggesting seasonal change. To identify the viruses, we searched the transcripts against a viral reference database using MegaBLAST with the most identified being bacteriophages infecting the marine bacteria. However, we also revealed an abundance of transcripts associated with diverse herpesviruses in the two transcriptomes, indicating the presence or possible threat of infection of fish in the sea around Jeju Island. This data is valuable for the study of marine microbial communities and for identifying possible viral pathogens.

• Korean Journal of Construction Engineering and Management
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• v.15 no.3
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• pp.23-30
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• 2014

Overseas construction orders of domestic companies had been increased significantly in a short period from $10.9 billion in 2005 to $64.9 billion in 2012. However experts are concerned that quality of growth will not support quantitative growth. This study suggest concentrate competencies for not only winning in bid but also successful execution of domestic companies. 4 Steps which are Pre-Marketing, Bid-Preparation, Bidding, Contract Phase are defined for competency assessment. After drawing specific tasks by phase, survey asking importance and capability of the tasks to the person in charge of bidding and site was conducted by utilizing Likert 7 point scale. According to the result of IPA analysis, domestic construction companies need to ensure internal competencies; Project risk review, Bid team organization, and external competencies; Client needs grasp, Localization strategy, Cooperation establishment. Hearafter domestic construction companies will be able to utilize the result of the study for setting bidding strategy for winning in bid and improving earning rate.