• The International Journal of Costume Culture
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• v.7 no.1
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• pp.40-47
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• 2004

The purpose of this research was to examine the degradation rate of alizarin in accelerated degradation conditions using the GC-MS quantitative analysis. Alizarin dye solution (2.5 x 10/sup -3/ M conc.) were kept in 150℃ oven for total of 7 days and the degradation rate was examined each day. 2.5 × 10/sup -4/M conc. alizarin dye solution was mixed with H₂O₂ according to [H₂O₂]/[dye] ratio 40 and were kept under 365㎚ UV for 2 hours, analyzed after 0, 30, 60, 90, 120min using the GC-MS. Gas chromatogram showed alizarin peak at 9.96 - 10.13 min. retention time range and residual peaks in the wide range from 9.6 to 11.1 min. Oven degradation exhibited an initial decrease in the amount of alizarin, which was followed by increasing amount in 4/sup th/ day. The decrease in the alizarin was significantly shown by the 7/sup th/ day. Same pattern was also observed in the H₂O₂/UV/O₂ degradation samples and was verified ed by the UV-VIS spectra. The differences in the amount of alizarin between 1/sup st/ day and 4/sup th/ day samples, 4/sup th/ day and 7/sup th/ day samples, and Control and 7/sup th/ day samples of the oven degradation were significant at alpha .20.

• Molecules and Cells
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• v.28 no.4
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• pp.397-401
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• 2009

Flavonoids are a group of polyphenolic compounds that have been recognized as important due to their physiological and pharmacological roles and their health benefits. Glycosylation of flavonoids has a wide range of effects on flavonoid solubility, stability, and bioavailability. We previously generated the E. coli BL21 (DE3) ${\Delta}pgi$ host by deleting the glucose-phosphate isomerase (Pgi) gene in E. coli BL21 (DE3). This host was further engineered for whole-cell biotransformation by integration of galU from E. coli K12, and expression of calS8 (UDP-glucose dehydrogenase) and calS9 (UDP-glucuronic acid decarboxylase) from Micromonospora echinospora spp. calichensis and arGt-4 (7-O-glycosyltransferase) from Arabidopsis thaliana to form E. coli (US89Gt-4), which is expected to produce glycosylated flavonoids. To test the designed system, the engineered host was fed with naringenin as a substrate, and naringenin 7-O-xyloside, a glycosylated naringenin product, was detected. Product was verified by HPLC-LC/MS and ESI-MS/MS analyses. The reconstructed host can be applied for the production of various classes of glycosylated flavonoids.

• Journal of Korean Society for Quality Management
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• v.44 no.1
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• pp.77-94
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• 2016

Purpose: This paper reviews Type 1 Gage study, Gage R&R study, and procedure of ISO 22514-7 for assessing a measurement system, and discusses some relationships between the measurement capability indices. Methods: The gage capability index $C_g$ of Type 1 Gage study, precision-to-tolerance ratio PTR of Gage R&R study, and measurement performance ratio $Q_{MS}$ and $Q_{MP}$ of ISO 22514-7 are considered in this paper. Results: This paper derives the relationships between $C_g$ and $Q_{MS}$, PTR and $Q_{MP}$, and $Q_{MS}$ and $Q_{MP}$, respectively, and discusses the acceptance conditions for each procedures. Conclusion: The measurement capability analysis is the first step for the quality improvement of the manufacturing processes. Therefore the result of this study provides a helpful guidelines for assessing the measurement system, enabling proper evaluation of manufacturing processes.

• Current Research on Agriculture and Life Sciences
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• v.6
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• pp.75-85
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• 1988

The concentration of 50 mM Nacl inhibited the shoot growth of P. nigra ${\times}$ maximowiczii in viro culture. The punctured leaves have produced so many individuals on MS basal medium with cytokinin. Especially MS basal medium with BAP 0.8mg/l showed the best shoot performance in which the average number of shoots were 127.6. For selection of NaCl tolerance shoot of poplar, punctured leaves were inoculated on MS basal medium with BAP 0.8mg/l and various concentrations of NaCl(from 10 mM to 100 mM graded by 10 mM). On the medium with over 50mM of NaCl, 13.7 to 15.7 shoots were obtained. Especially on the medium with go mM and 100mM. 10.7 and 8.3 shoots, respectively. The shoots derived from control medium (non-NaCl) were depressed growth, while the selected shoots from MS with NaCl showed good growth performance on MS basal medium with 50 mM of NaCl. From this results, we suggested that the possibility of in vitro selection to tolerance for inorganic salts in forest tree species.

• Korean Journal of Medicinal Crop Science
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• v.5 no.1
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• pp.49-55
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• 1997

This study was conducted to establish mass propagation system from the tissue culture using immature embryos in Eleutherococcus senticosus. Immature embryos from seeds were removed under the microscope and placed on MS, $MSB_5\;and\;B_5$ medium containing several plant growth regulators. While the calli were well formed on media containing 2 mg/l of 2, 4-D, 2 mg/l of 2, 4-D and 0.7 mg/l of TDZ, shoot regeneration was better on MS medium with combinations of high concentrations of TDZ and low concentrations of 2, 4-D. Treatment of 2, 4-D alone was better than treatment of TDZ alone in callus induction, but plant regeneration was reversed. The results of callus formation and shoot regeneration on $MSB_5\;and\;B_5$ media were similar to those of MS media. The rate of callus formation was nearly 100% when 2, 4-D was added to $B_5$, medium on concentration of 2 mg/l or 0.7 mg/l. TDZ showed very significant effect on the formation of multiple shoots.

• Analytical Science and Technology
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• v.21 no.1
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• pp.41-47
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• 2008

Although liquid-liquid extraction (LLE) method has been used routinely for the analysis of amphetamine-like drugs (amphetamine; AP, methamphetamine; MA, 3,4-methylenedioxyamphetamine; MDA, 3,4-methylenedioxymethamphetamine; MDMA, 3,4-methylenedioxyethylamphetamine; MDEA), a solid-phase extraction (SPE) method, which can be automated, was applied for the simultaneous determination by GC/MS in human urine. Urine samples (3 mL) and 0.1 M phosphate buffer (1 mL, pH 7.0) were extracted by an automated SPE system. The eluent was evaporated, derivatized with trifluoroacetic anhydride (TFAA), and analyzed by GC/MS. The calibration curves was linear with correlation coefficient ($r^2$) above 0.994 in the ranges of 34.0 (AP), 28.0 (MDA)~1000.0 ng/mL for AP, MDA, and 50.0~2000.0 ng/mL for MA, MDMA, and MDEA. The limits of detection ranged from 4.0 to 10.0 ng/mL, and the limits of quantitation ranged from 12.0 to 34.0 ng/mL. The relative recoveries were 93.5~107.7 %. The precisions and accuracies were 1.9~14.8 % and -8.7~14.8 %, respectively. The present method was successfully applied to identify the MA or Ecstasy (MDMA) abusers in exact as well as rapid.

• Journal of Environmental Science International
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• v.6 no.5
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• pp.481-488
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• 1997

Microorganisms capable of degrading trlchloroethylene(TCEI using phenol as a induction substrate were isolated from industrial effluents and soil. The strain MS-64K which had the highest blodegradablllty was identified as the genus Micrococcus. The optimal conditions of medium for the growth and blodegadatlon of trlchloroethylene were observed as follows; the initial pH 7.0, trlchloroethylene 1, 000ppm as the carbon source, 0.2% ${(NH_4)}_2SO_4$, as the nitrogen source. respectively. Lag period and degradation time on optimal medium were shorter than those on Isolation medium. Growth on the optimal medium was Increased. Addition of 0.1% Triton X-100 Increased the growth rate of Micrococcus sp. MS-64K, but degradation was equal to optimal medium. Trlchloroethylene degradation by Micrococcus sp. MS-64K was shown to fit logarithmic model when the compound was added at initial concentration of 1, 000ppm.

• Bulletin of the Korean Chemical Society
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• v.24 no.12
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• pp.1805-1808
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• 2003

The simultaneous determination of As(III), As(V), and DMA has been performed by ion chromatography (IC) coupled with inductively coupled plasma-mass spectrometry (ICP-MS). The separation of the three arsenic species was achieved by an anionic separator column (AS 7) with an isocratic elution system. The separated species were directly detected by ICP-MS as an element-selective detection method. The IC-ICP-MS technique was applied for the determination of arsenic species in a NIST SRM 1643d water sample. An As(III) only was detected in the sample. The detection limits of As(III), As(V) and DMA were 0.31, 0.45, and 2.09 ng/mL, respectively. It was also applied for the determination of arsenic species in a human urine obtained by a volunteer, and three arsenic species were identified. The determination of total As in human urines that were obtained from 25 volunteers at the different age was also carried out by ICP-MS.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.60 no.2
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• pp.325-329
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• 2011

This paper introduces the 10b 1MS/s SAR ADC with double sampling technique to reduce the power consumption. The SAR ADC is implemented in CMOS 1P8M 65nm technology and occupies 0.11um2. The maximum sampling rate is 1MS/s. The simulated SNDR and SFDR are 55.6dB and 62.7dB at 484kHz input frequency, respectively. The implemented data converter consumes 507uW with 1.2-V supply.

• Korean Journal of Pharmacognosy
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• v.48 no.4
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• pp.320-328
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• 2017

Yongdamsagan-tang has been used to treat the urinary disorders, acute- and chronic-urethritis, and cystitis in Korea. In this study, an ultra-performance liquid chromatography-electrospray ionization-mass spectrometry (UPLC-ESI-MS/MS) method was established for simultaneous analysis of the 20 bioactive marker compounds, geniposidic acid, chlorogenic acid, geniposide, liquiritin apioside, acteoside, calceolarioside B, liquiritin, nodakenin, baicalin, liquiritigenin, wogonoside, baicalein, glycyrrhizin, wogonin, glycyrrhizin, wogonin, saikosaponin A, decursin, decursinol angelate, alisol B, alisol B acetate, and pachymic acid in traditional herbal formula, Yongdamsagan-tang. Chromatographic separations of all marker compounds were conducted using a Waters Acquity UPLC BEH $C_{18}$ analytical column ($2.1{\times}100mm$, $1.7{\mu}m$) at $45^{\circ}C$ using a mobile phase of 0.1% (v/v) formic acid in water and acetonitrile with gradient elution. The MS analysis was performed using a Waters ACQUITY TQD LC-MS/MS coupled with an electrospray ionization source in the positive and negative modes. The flow rate was 0.3 mL/min and injection volume was $2.0{\mu}L$. The correlation coefficient of 20 marker compounds in the test ranges was 0.9943-1.0000. The limits of detection and quantification values of the all marker components were 0.11-6.66 and 0.34-19.99 ng/mL, respectively. As a result of the analysis using the optimized LC-ESI-MS/MS method, three compounds, geniposidic acid (from Plantaginis Semen), alisol B (from Alismatis Rhizoma), and pachymic acid (from Poria Sclerotium), were not detected in this sample. While the amounts of the 17 compounds except for the geniposidic acid, alisol B, and pachymic acid were $0.04-548.13{\mu}g/g$ in Yongdamsagan-tang sample. Among these compounds, baicalin, bioactive marker compound of Scutellariae Radix, was detected at the highest amount as a $548.13{\mu}g/g$.