Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Metabolic Engineering of Escherichia coli for the Biological Synthesis of 7-O-Xylosyl Naringenin

  • Simkhada, Dinesh (Department of Pharmaceutical, Institute of Biomolecule Reconstruction, Sun Moon University) ;
  • Kim, EuiMin (Department of Pharmaceutical, Institute of Biomolecule Reconstruction, Sun Moon University) ;
  • Lee, Hei Chan (Department of Pharmaceutical, Institute of Biomolecule Reconstruction, Sun Moon University) ;
  • Sohng, Jae Kyung (Department of Pharmaceutical, Institute of Biomolecule Reconstruction, Sun Moon University)
  • Received : 2009.07.27
  • Accepted : 2009.08.26
  • Published : 2009.10.31
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Abstract

Flavonoids are a group of polyphenolic compounds that have been recognized as important due to their physiological and pharmacological roles and their health benefits. Glycosylation of flavonoids has a wide range of effects on flavonoid solubility, stability, and bioavailability. We previously generated the E. coli BL21 (DE3) ${\Delta}pgi$ host by deleting the glucose-phosphate isomerase (Pgi) gene in E. coli BL21 (DE3). This host was further engineered for whole-cell biotransformation by integration of galU from E. coli K12, and expression of calS8 (UDP-glucose dehydrogenase) and calS9 (UDP-glucuronic acid decarboxylase) from Micromonospora echinospora spp. calichensis and arGt-4 (7-O-glycosyltransferase) from Arabidopsis thaliana to form E. coli (US89Gt-4), which is expected to produce glycosylated flavonoids. To test the designed system, the engineered host was fed with naringenin as a substrate, and naringenin 7-O-xyloside, a glycosylated naringenin product, was detected. Product was verified by HPLC-LC/MS and ESI-MS/MS analyses. The reconstructed host can be applied for the production of various classes of glycosylated flavonoids.

Keywords

Acknowledgement

Supported by : Ministry of Science and Technology

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