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Recent study of Acupuncture in Treatment of Urianry Disturbance (배뇨장애(排尿障碍)에 대한 침구치료(鍼灸治療)의 연구동향(硏究動向))

  • Kim, Kyung-tai;Ko, Young-jin;Kim, Yong-suk;Kim, Chang-hwan
    • Journal of Acupuncture Research
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    • v.22 no.3
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    • pp.123-135
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    • 2005
  • Objective : The aim of this study was to rivew systemically literature and clinical trials in the treatment of urinary incontinence or lower urinary tract syndrome(LUTS). Methods : Computerized literature searches were carried out on two electronic database, and computerized searching on some korea oriental medicine journals in library of Kyung-Hee Medical center. Results : 1. Three reports of review study, six reports of experimental study and fourteen reports of clinical trials were collected and reviewed. Three reports of review study were all printed in the korea oriental medicine journal. From 2000, researches and studies have been increased in quantity and improved in quality. 2. Urinary disturbance include variable symptoms of lower urinary tract symptoms, urinary incontinence, in theaspect of Oriental medicine these symptoms are anurin, dysuria, urinary incontinence, nochumal enuresis, uracratia and so on. 3. Roughly physiological procedure of Acupuncture in Treatment of Urianry Disturbance may be that effect of acupuncture stimulation for parasympathetic nerve, sleep-arousal system in cerebrum, pontine/spinal urination center and pudendal/pelvic nerve affect bladder in expansion of bladder capacity, inhibition of urinary contraction and affection in periurethral muscle by continuous excitement of spinal annular circuit and synapse of neuron. 4. Clinical result for acupuncture treatment in urinary disturbance is summarized that acupuncture treatment in urianation disturbance of Neurogenic Bladder, Incontinence, Cycitis, Nocturnal Enuresis, Prostatitis/Pelvic Pain Syndrom and so on is significant clinical trials and technique. Conclusion : Hereafter, in the old age society these variable urinary disturbance patients are increased and desire of treatment may be also increased. So study of various and formal treatment and tecnnique is needed.

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COMPARATIVE STUDY ON FLUORIDE RELEASE AND RE-UPTAKE CAPACITY OF SEVERAL FLUORIDE-RELEASING RESTORATIVE MATERIALS (수종의 수복재의 불소 적용법에 따른 불소 유리에 관한 비교 연구)

  • Lee, Yeon-Ho;Yoo, Seung-Hoon;Kim, Jong-Soo
    • Journal of the korean academy of Pediatric Dentistry
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    • v.33 no.1
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    • pp.25-34
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    • 2006
  • Fluoride released from dental restorative materials effectively declines the incidence and activity of dental caries and inhibits tooth demineralization. This study investigated the fluoride release and uptake characteristics of one composite resin $(Z-250^{TM})$ three glass ionomer-based restorative material ($Dyract^{(R)}$ AP, Fuji II $LC^{(R)}$, Fuji IX GP $Fast^{(R)}$) Forty discs(6mm diameter and 1mm height) were prepared for each material. Each disc was immersed in 5ml of distilled water within polyethylene vial and stored at $37^{\circ}C$. The distilled water was changed every 24 hours and the release of fluoride was measured for 31 days. At the end of this period, each specimen was subjected to one of four treatments : (A) no fluoride treatment (control), (B) application of a fluoride dentifrice (500ppm) for three minutes three times; (C) application of the 1.23% acidulated phosphate fluoride(APF) foam for one minute once, (D) the same regimen as (B), plus application of the APF foam for one minute once. Then, all samples were reassessed for an additional 7 days. For all samples, the greatest fluoride release was observed after the first day of the study but diminished with time. On the 7th day of the study, fluoride release level was stabilized. Fuji II $LC^{(R)}$ and Fuji IX GP $Fast^{(R)}$ released higher amount of fluoride than other materials ; however, no statistically significant difference was found from Fuji II $LC^{(R)}$ and Fuji IX GP $Fast^{(R)}$. The amount of fluoride of $Dyract^{(R)}$ AP, Fuji II $LC^{(R)}$ and Fuji IX GP $Fast^{(R)}$ was increased after fluoride treatment, and diminished with time.

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Detection of Pyrazinamide-Resistant Mycobacterium tuberculosis is by PCR-SSCP of pncA Gene (pncA 유전자 PCR-SSCP법을 이용한 결핵균 Pyrazinamide 내성의 진단)

  • Shim, Tae-Sun;Kim, Young-Whan;Chin, Jae-Yong;Lim, Chae-Man;Lee, Sang-Do;Koh, Youn-Suck;Kim, Woo-Sung;Kim, Dong-Soon;Kim, Won-Dong
    • Tuberculosis and Respiratory Diseases
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    • v.45 no.6
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    • pp.1178-1187
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    • 1998
  • Background : Recently the incidence of tuberculosis is increasing in many countries and control of the disease is further threatened by the emergence of multi-drug resistant tuberculosis. So rapid detection of drug resistance is very important. Pyrazinamide (PZA) is a first-line chemotherapeutic agent for tuberculosis. Now in Korea, we perform PZase activity test instead of actual pyrazinamide susceptibility test for the detection of PZA resistant M. tuberculosis. Recently the pncA gene, encoding the PZase of M. tuberculosis, was completely sequenced. And it was reported that the mutation of pncA gene would be associated with PZA resistance of M. tuberculosis. Therefore we performed this study to evaluate the possibility for the rapid detection of PZA resistant M. tuberculosis using PCR-SSCP of pncA gene. Method : 44 cultured clinical isolates of M. tuberculosis, BCG Tokyo strain. BCG French strain, and one M. bovis isolate were studied. We used H37Rv as the reference strain, The PZase activity test was done at the reference laboratory of Korean Tuberculosis Institute. DNA was extracted by bead-beater method and 561 bp fragment including pncA gene was amplified by PCR. The PCR product were digested by BstB I enzyme. SSCP was done using MDE gel. Of the 44 strains of M. tuberculosis, 22 strains were PZase-positive and other 22 strains were PZase negative. Results : Of the 22 PZase positive strains, 18 strains(82%) showed the same mobility compared with that of H37Rv and 4(18%) showed different mobility. Of the 22 PZase-negative strains, 19(86%) strains showed the same mobility pattern compared with that of H37Rv and 3(14%) showed different mobility. Naturally PZA-resistant BeG-French strain, BCG-Tokyo strain, and one M. bovis isolate showed the same band pattern each other, but their mobility were different from that of H37Rv. The results of PZase activity test and PCR-SSCP of pncA of M. tuberculosis were statistically significantly correlated each other (p<0.01). Conclusion : The PCR-SSCP after BstB I restriction of pncA gene of M. tuberculosis may be a useful method for the rapid detection of PZA-resistant M. tuberculosis.

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Characteristics of Glutinous Waxy Maize Hybrids (재래종(在來種) 찰옥수수 교잡종(交雜種)의 특성비교(特性比較))

  • Lee, Won Koo;Ji, Hee Jeong;Baek, Man Kee;Park, Ki Sun;Lee, Hee Bong;Choe, Bong Ho
    • Korean Journal of Agricultural Science
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    • v.19 no.2
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    • pp.129-135
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    • 1992
  • Characteristics of ten glutinous maize hybrids developed at the Genetics and Breeding Lab. of Department of Agronomy, College of Agriculture, Chungnam National University were compared. Inbred lines used in the development of the ten hybrids were all originated from the Korean local maize lines which were collected in 1980s and they were developed by ear-to-row method. All the inbreds used had very unique plant characteristics such as plant height, ear height, ear length, ear diameter, 100 kernel weight and kernel color. The highest inbred in plant height was Jewon and the Jewon was also highest in ear height. While the ear of Jewon inbred was slender and slimmy, the ear of Dangjin inbred was rather short and promp. 100 kernel weight of the Bosung inbred was highest and the kernel weight of Chilbo was lightest. Kernel color of Jewon, Bosung and Danyang was white and the kernel color of Yunghee and Chilbo was purple. Dangjin had yellow kernel color. A total of 11 hybrids including one check hybrid developed by the Crop Experiment Station, ADR were included in performance trial. The hybrids which showed high grain yield were Dangjin/Bosung, Sosung/Dangjin and Dangjin/Chilbo. These hybrids were about 10% higher than the check in yield capacity. Each hybrid had also unique characteristics such as plant height, ear height and flowering date.

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Botanical Characteristics and Effect of Weed Control by Herbicide in Barley (제초제 처리에 의한 보리의 작물학적 특성)

  • Hong, Bum-Yong;Choi, Yun-Pyo;Cha, Hui-Jung;Ju, Jung-Il;Lee, Hee-Bong
    • Korean Journal of Agricultural Science
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    • v.35 no.2
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    • pp.119-126
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    • 2008
  • This study was carried out to find the botanical characters and effect of weed control for herbicides treatment after barley sowing. Barley variety used in study was "Yuyeon", hood-type awn for whole crop forage use, which developed at RDA in 2004. Sowing date was on 10th October, 2007, and was drilled as $60{\times}20cm$. Herbicides were treated on 2th day after barley sowing. Measured items were number and kinds of weeds at each treatment and growth characteristics and grain yield in barley. Cerastium holosteoides var. hallaisanense among weeds surveyed at tillering stages in Butachlor treatment were highly appeared, but Alopecurus aequalis var. amurensis did not appeared while Alopecurus aequalis var. amurensis was 39 plants and Capeslla bursa-pastoris was 37 plants in check. Occurrence of the Capeslla bursa-pastoris at primary survey and Cerastium holosteoides var. hallaisanense in ripening stage were high, while Alopecurus aequalis var. amurensis did not emerged. Botanical characteristics of barley each as plant height, number of tillers and spiklets per plant. surveyed at ripening stage were higher than check. Grain yield per 10a was the highest at Alachlor among treatments, while dry matter of leaf and culm were similar among treatments. Therefore, effect of yield increasing of barley by herbicide after sowing was reconfirmed through this study. Especially, effect of Alachlor was lightly high compared with others.

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The physicochemical characteristics and antioxidant capacities of commercial tea products from Phellinus baumii, Ganoderma lucidum (상황버섯과 영지버섯 차류 제품의 이화학적 특성 및 항산화능)

  • Kim, Ha-Na;Son, Eun Ji;Chung, Shin-Kyo
    • Food Science and Preservation
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    • v.24 no.1
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    • pp.153-157
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    • 2017
  • This study was conducted to investigate the physicochemical characteristics, antioxidant capacities of Phellinus linteus and Ganoderma lucidum commercial tea products. The physicochemical characteristics included pH, Hunter's color values, soluble solid contents, evaporation residues, and ${\beta}$-glucan contents. The antioxidant capacities were measured by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities, ferric reducing antioxidant power (FRAP), and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activities, total phenolic contents (TPC), and total flavonoid contents (TFC). The pH, soluble solid contents, evaporation residues, and ${\beta}$-glucan contents were in the range of 4.43-7.05, $0.40-0.73^{\circ}Brix$, 62.04-258.84 mg/100 g, and 15.51-62.32 mg%, respectively. Hunter's color values (L, a, and b) indicated 41.76-55.02, -0.49-5.06, and 17.41-28.32, respectively. The antioxidant capacities showed $32.63-367.81{\mu}M$ GAE (DPPH radical scavenging activities), $321.86-1,035.19{\mu}M$ TE (FRAP), $703.50-1,091.83{\mu}M$ (ABTS radical scavenging activities), $286.56-916.00{\mu}M$ (TPC), and $85.33-635.33{\mu}M$ (TFC). Overall, P. linteus liquid tea 2 (PL2) and G. lucidum liquid tea 1 (GL1) showed high antioxidant capacities (p<0.05). The TPC and TFC were highly correlated with DPPH radical scavenging activities, FRAP, and ABTS radical scavenging activities (r=0.7298-0.9743), but the ${\beta}$-glucan contents were not correlated well with antioxidant activities tested (r=0.3146-0.6663).

Evaluations on Deodorization Effect and Anti-oral Microbial Activity of Essential Oil from Pinus koraiensis (잣나무 정유의 소취효과 및 구강균에 대한 항균활성 평가)

  • Hwang, Hyun Jung;Yu, Jung-Sik;Lee, Ha Yeon;Kwon, Dong-Joo;Han, Woong;Heo, Seong-Il;Kim, Sun Young
    • Korean Journal of Plant Resources
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    • v.27 no.1
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    • pp.1-10
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    • 2014
  • Essential oils of various plants have been known for potential biological effects such as antibacterial, antifungal, spasmolytic, antiplasmodial activities and insect-repellent property. Recently, the essential oils have attracted considerable interest in oral disease therapy. This essential oil has been known as being effective on easing sick house syndrome, giving forest aroma therapy effect and acting as repellent against pest. The essential oil of Pinus koraiensi, a native plant from Hongcheon-gun, Gangwon-do, was obtained by hydrodistillation. In light of its medicinal importance, in this study its composition, antibacterial activity and the reducing effect of offensive odor have been analyzed. The composition of essential oil was determined by GC and GC-MS. We have identified 14 compounds, of which 1R-${\alpha}$-pinene (19.38 %), 3-carene (10.21 %), camphene (9.82 %), limonene (9.00 %), bicyclo[2,2,1] heptan-2-ol (8.76 %) and ${\beta}$-phellandrene (7.98 %) were the main components. Essential oils from P. koraiensis, Chamaecyparis obtusa, Abies holophylla and Pinus densiflora were compared in terms of alleviating effect of malodors caused from formaldehyde, ammonia, trimethylamine and methylmercaptan. P. koraiensis essential oil was found to decrease the amounts of ammonia and trimethylamine by 75.17 % and 77.36 %, respectively. Antibacterial activity against Streptococcus mutans and Streptococcus sobrinus, which were known as oral cavity inducer, was investigated using the paper disc agar diffusion method. The inhibition zone was observed against S. mutans (5.97 mm) and S. sobrinus (1.40 mm), respectively. P. koraiensis essential oil shown effective deodorization and inhibitory activity against oral cavity in this study might be potential material in oral sanitary industry.

Biological activities of Fusarium isolates from soil and plants (토양 및 식물체로부터 분리한 Fusarium속 균주들의 생물활성)

  • Park, Joong-Hyeop;Choi, Gyung-Ja;Kim, Heung-Tae;Hong, Kyung-Sik;Song, Cheol;Kim, Jin-Seog;Kim, Jeong-Gyu;Cho, Kwang-Yun;Kim, Jin-Cheol
    • The Korean Journal of Pesticide Science
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    • v.4 no.3
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    • pp.19-26
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    • 2000
  • In order to select potent bioactive isolates, 70 Fusarium isolates obtained from soil and 21 plant species were screened by antifungal, insecticidal, herbicidal, and duckweed bioassays after culturing in potato dextrose broth and rice solid media. Eight (11.4%) of the 70 liquid broth cultures showed disease-controlling activities more than 80% against at least one of the 6 plant diseases tested. Fusarium sp. FO-68 isolate exhibited the most potent antifungal activity; it controlled rice blast, wheat leaf rust, and barley powdery mildew with control values more than 95%. Out of 70 solid cultures, 21 (30.0%) controlled at least one plant disease more than 80% and F. equiseti FO-68 isolate showed disease-controlling activities more than 95% against 3 plant diseases such as rice blast, tomato late blight, and wheat leaf rust. As for tile insecticidal activities, 2 liquid and 1 solid cultures showed potent insecticidal activities against pest insects more than 80%, Liquid cultures of F. oxysporum FO-61 and Fusarium sp. FO-80 isolates exhibited insecticidal activities more than 80% against green peach aphid and diamondback moth, respectively. The solid culture of Fusarium sp. FO-510 isolate had 80% insecticidal activity against green peach aphid. However, none of liquid and solid cultures of the 70 Fusarium isolates showed potent herbicidal activities against 10 upland weeds. As the results of duckweed assay, 3 liquid cultures showed 70% growth inhibitory activity at concentrations less than 1.25% of culture supernatants and 9 solid cultures had a potent inhibitory activity against duckweed growth. On the other hand, there was a significant correlation between antifungal activities and herbicidal activities against duckweed of both liquid and solid cultures of tile 70 Fusarium isolates.

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Distribution Characteristics of Heavy Metals for Tidal Flat Sediments in the Saemankeum Area (새만금 갯벌의 중금속 분포 특성)

  • KIM Jong-Gu;YOO Sun-Jae;CHO Eun-Il;AHN Wook-Sung
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.36 no.1
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    • pp.55-61
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    • 2003
  • This study was conducted to evaluate distribution and behavior characteristics of heavy metals for tidal flat sediments in the Saemankeum area. The value of heavy metal contents in the tidal flat sediments were higher than that surveyed in 1994, but showing that it's lower when compared with the value of Shiwha lake. Enrichment factors in the tidal flat sediments showed below 1 except for Pb and Zn. Metal excesses was appeared that Cu, Cr, Fe, Mn lacked and Pb, Zn were high. The correlation analysis between heavy metals and organic matters was found high positive relationship (r=0.424$\~$0.839), especially correlation coefficient between ignition loss and oxide Al, Fe, Mn showed high positive relationship above 0.7. The correlation analysis between Brain size and hear metals was found that as grain size was small, heavy metals contents were increased. Correlation coefficient between silt and heavy metals except for Cu, Pb showed high positive relationship as 0.552$\~$0.732.

The Study on the Factors for Detection of Renal Stone on Ultrasound (초음파 검사에서 신장 결석의 검출 요인에 관한 연구)

  • Sim, Hyun-Sun;Jung, Hong-Ryang;Lim, Cheong-Hwan
    • Journal of radiological science and technology
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    • v.29 no.1
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    • pp.1-6
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    • 2006
  • Purpose: Renal stones are common and typically arise within the collecting system. The renal sinus are contains the collection system, the renal vessels, lymphatcs, fat, and fibrous tissue. Because of the compression of all the large echoes in signal processing, the echo from the renal stone generally cannot be distinguished from large echoes emanating from normal structures of the renal sinus. Use of ultrasonography has been difficult for detecting small renal stone without posterior shadowing and chemical composition of stone. The aim of study was measuring for posterior acoustic shadowing to a stone for various scan parameter and it examines a help in renal stone diagnosis. Material & Methods: The stone was place on sponge examined in a water bath with a 3.5MHz or 7.5MHz transducer(LOGIQ 400, USA). First, tested a variety of gain. Second, tested a variety of dynamic range. Third, tested a variety of focal zone. Fourth, measuring of the echo level for low and high frequency for depth. Results: 1) Average echo level was 98 for low total gain(10 dB) and was 142 for high total gain(40 dB). Posterior acoustic shadowing of renal stone was clear for low gain. 2) Average echo level was 129 for low dynamic range(42 dB) and was 101 for high dynamic range(72 dB). Posterior acoustic shadowing of renal stone was clear for high dynamic range. 3) When stone is in focal zone of transducer, definite posterior acoustic shadow is identified. 4) Stone was clear appeared for high frequency(7.5 MHz) than low frequency(3.5 MHz) and it is not distorted. Conclusion: The demonstration of an posterior acoustic shadow of renal stone dependents on several technical factors such as gain, dynamic range, focus, and frequency. This various factors are a help in renal stone diagnosis.

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