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Biochemical and Microbiological Changes of Hard Clam Shikhae During Fermentation (백합식해 발효 중 생화학적 및 미생물학적 특성 변화)

  • Koo, Jae-Geun;Yoo, Jung-Hee;Park, Kwon-Sam;Kim, Sun-Young
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.42 no.6
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    • pp.569-573
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    • 2009
  • The biochemical and microbiological changes of the hard clam shikhae were studied during fermentation at $4-18^{\circ}C$ for 45 days. For preparation of the shikhae, the shucked hard clams were blanched into 2% saline solution and were soaked in seasoning solution before mixing with salt, cooked grain and spices. During fermentation, the initial pH steadily decreased from 5.0 to 4.6, but $NH_2-N$ and VBN concentrations increased to 127 mg/100 g and 27.0 mg/100 g, respectively. Alanine, taurine, glutamic acid, and aspartic acid concentrations increased, but arginine concentration decreased by fermentation. The major organic acids of the fermented shikhae were lactic acid, succinic acid and acetic acid. The major free sugar were maltose, glucose and fructose. The concentration of total viable cell ($2.1\times10^5$ CFU/g) and proteolytic bacteria ($1.2\times10^5$ CFU/g) increased to $4.4\times10^8$ CFU/g and $9.8\times10^7$ CFU/g, respectively until day 15 and then slightly decreased. The concentration of yeast ($2.4\times10^3$ CFU/g) increased to $1.6\times10^7$ CFU/g until day 25, but lactic acid bacteria ($5.0\times10^8$ CFU/g) increased to $5.0\times10^8$ CFU/g until day 9. Vibrio species was not detected on the TCBS agar during fermentation.

Evaluating Cultured Sea Mussels Mytilus edulis Extractions Methods and Extract Quality Characteristics (진주담치(Mytilus edulis) 추출물의 제조 및 품질특성)

  • Kim, Seon-Geun;Cho, Jun-Hyun;Hwang, Young-Sook;Lee, In-Seok;Oh, Kwang-Soo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.50 no.6
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    • pp.650-655
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    • 2017
  • Extraction methods for cultured sea mussels Mytilus edulis and the quality characteristics of resulting extracts were investigated. The crude protein, carbohydrate and volatile basic nitrogen content of raw sea mussels was 15.2%, 1.9%, and 11.2 mg/100 g, respectively. Extracts were prepared using three different methods: hot-water extract (WE), scrap enzymatic hydrolysate extraction (SE), and complex extraction (CE). The respective extracts contained 5.5%, 8.6%, and 6.6% crude protein; 281.7, 366.0, and 343.0 mg/100 g amino nitrogen,: and 2.0%, 1.1% and 1.8% salinity. Their extraction yields were 689, 323, and 1,012 mL/kg. The CE method was superior to the traditional WE method in terms of extraction yield, amino-nitrogen content, and organoleptic qualities, but not odor. Active taste components were evaluated and the total free amino acid content of the WE and CE methods was 5,667.0 and 7,006.3 mg/100 g, respectively. The concentrations of major components (for WE and CE methods, respectively) were as follows: glutamic acid (1,244.0 and 955.4 mg/100 g), taurine (987.9 and 746.8 mg/100 g), glycine (721.2 and 847.0 mg/100 g), alanine (341.9 and 423.8 mg/100 g), arginine (265.5 and 376.5 mg/100 g), lysine (199.8 and 270.4 mg/100 g), and proline (253.9 and 220.3 mg/100 g). In conclusion, these results demonstrate that there is potential for using the CE method to expand the commercial utilization of sea mussels as a flavoring substance resource.

Ethics-Literacy Curriculum Modeling for Ethical Practice of 5G Information Professionals (5G 정보환경 정보전문가를 위한 윤리 리터러시 교육과정 모형연구)

  • Yoo, Sarah
    • Journal of the Korean BIBLIA Society for library and Information Science
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    • v.33 no.1
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    • pp.139-166
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    • 2022
  • Ethical Issues increase when people engage in smart technological systems such as 5G, IoT, Cloud computing services and AI applications. Range of this research is comparison of various literacy concepts and its ethical issues in considering of 5G features and UX. 86 research papers and reports which have been published within the recent 5 years (2017-2022), relating the research subject, are investigated and analyzed. Two results show that various literacies can be grouped into four areas and that some of common issues among those areas as well as unique issues of each area are identified. Based on the literature analysis, an Operational Definition of Ethics-Literacy is presented and the model of ethics-literacy curriculum supporting ethical behavior of 5G information professionals is developed and suggested.

Biological Hazard Analysis of Angelica gigas Nakai on Production and Marketing Steps (당귀의 재배 및 유통과정 중 생물적 위해요소 분석)

  • Park, Kyeong-Hun;Kim, Byeong-Seok;Lee, Jeong-Ju;Yun, Hye-Jeong;Kim, Se-Ri;Kim, Won-Il;Yun, Jong-Chul;Ryu, Kyoung-Yul
    • Korean Journal of Soil Science and Fertilizer
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    • v.45 no.6
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    • pp.1216-1221
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    • 2012
  • This study is aimed to investigate microbiological contamination of Angelica gigas Nakai. A total of 111 samples including root, soil, and irrigation water were collected from farms and market to detect aerobic bacteria, Bacillus cereus, coliform, Escherichia coli, Listeria monocytogenes,. Salmonella spp., and Staphylococcus aureus. The contaminations of aerobic bacteria, coliform, and Bacillus cereus in the root during cultivation were found 6.71 log CFU $g^{-1}$, 4.13 log CFU $g^{-1}$, and 3.54 log CFU $g^{-1}$, respectively. The contamination of coliform and B. cereus were detected in all steps from harvesting to processing, with the highest count recorded from the cutting step. In marketing, the contaminations of aerobic bacterial, coliform, and B. cereus were 5.5~6.0 log CFU $g^{-1}$, 2.4~2.6 log CFU $g^{-1}$, and 3.5~4.0 log CFU $g^{-1}$, respectively. Listeria monocytogenes, Salmonella spp, and Staphylococcus aureus were not detected in any of samples. This result indicated that hygienic soil management and post harvest management should be performed to reduce the contamination of hazard microorganisms and to produce safe agro-products.

Effect of Surface Treatment of Porcelain on Tensile Bond Strength (도재의 표면처리가 인장접착강도에 미치는 영향)

  • KIM, Shin-Geun;SUNG, Jae-Hyun
    • The korean journal of orthodontics
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    • v.26 no.3
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    • pp.301-307
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    • 1996
  • The purpose of this study was to evaluate the effect of surface treatment of porcelain on tensile bond strength. To accomplish this purpose, this study was carried out with 120 samples which were divided into 12 groups with each 10 samples, and the first group was not surface treated, groups 2 through 5 underwent single surface treatment, and groups 6 through 12 underwent compound surface treatment. The results were as follows : 1. In statistic, all the single surface-treated groups showed higher tensile bond strength than the non surface-treated group and the sandblasted group showed the highest tensile bond strength as $10.34{\pm}2.50MPa$. 2. All the compound surface-treated groups showed no noticeable difference in the tensile bond strength(9-11.5MPa). 3. In statistic, no significant difference was found between the sandblasted group and the compound surface-treated groups. 4. There was no fracture of porcelain while testing in this study. Above study demonstrated that compound surface treatment or sandblasing, if used single surface treatment, should be employed to guarantee successful clinical application.

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Physicochemical Properties and Antioxidant Activities of Different Parts of Kkujippong (Cudrania tricuspidata Bureau) from Miryang (밀양산 꾸지뽕 나무(Cudrania tricuspidata Bureau)의 부위별 이화학적 특성 및 항산화 활성)

  • Choi, Duck-Joo;Lee, Yun-Jung;Kim, Youn-Kyeong;Kim, Mun-Ho;Choi, So-Rye;Kim, Soung-Soo;Youn, Aye-Ree
    • Korean journal of food and cookery science
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    • v.31 no.4
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    • pp.510-514
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    • 2015
  • Various parts of Kkujippong (Cudrania tricuspidata Bureau) tree (leaves, stems, roots, and fruit) were evaluated to determine their properties. The general components of Kkujippong tree were fat 1.3-5.9 g /100 g, protein 6.3-18.7 g /100 g, and ash 2.8-9.0 g /100 g. The fiber content of the stem was the highest amount at 69.2 g/100 g, followed by protein in the roots (49.9), leaves (45.5), and fruit (15.6). In addition, Kkujippong tree was found to contain high concentrations of several minerals (Ca, Fe, Mg, Na, P, Zn) in the leaves. The total polyphenol content was significantly higher in the leaves than in other areas at 571.3 mg% (p<0.05). Electron donating ability was also highest in the leaves at 83.55% (p<0.05).

Studies on the Utilization of Nitrogen by Korean Native Goat I Effect of Different Levels of Crude Protein Intake on the Nitrogen Retention (한국재래산양(韓國在來山羊)의 질소이용(窒素利用)의 관한 연구(硏究) I. 단백질(蛋白質) 급여수준(給與水準)이 질소축적(窒素蓄積)에 미치는 영향(影響))

  • Kwon, Soon Ki;Huh, Won
    • Korean Journal of Agricultural Science
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    • v.7 no.2
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    • pp.81-86
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    • 1980
  • This experiment was carried out to evaluate the effect on different levels of crude protein intake according to sex and body weight on the nitrogen retention, and the protein requirement for feeding standard of the Korean Native Goat. The results obtained from the study were as follows. 1. The nitrogen retention rate when the female goat at 6 months age (13kg) had taken native grass hay 40%(126g) and flaking barley 60%(186g) was 36 percent, and when the intake matter was native grass hay 55%(226g) and flaking barley 45%(186g) was 25.6 percent. 2. The nitrogen retention rate when the meal goat at 5 months age (11kg) had taken native grass hay 47%(245g), flaking barley 29%(153g) and soybean meal 24%(126g) was 52.4 percent and when the intake was native grass hay 42%(206g) and flaking barley 58%(279g) was 40.7 percent. But if the in take matter was only (328g) native grass hay (crude protein 8%) it was -23.9 percent. 3. Maintenance nitrogen requirement of Korean native goat were estimated about 5g per day to 11 or 13kg live weight.

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Clinico-pathological studies on the experimental cadmium pisoning in dogs (실험적 카드뮴 중독견의 임상병리학적 관찰)

  • Lee, Sang-gwan;Lee, Hyun-beom
    • Korean Journal of Veterinary Research
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    • v.36 no.2
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    • pp.471-482
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    • 1996
  • These experiments were undertaken in order to find out the useful clinicopathological diagnostic methods of cadmium poisoning in dogs. Twenty-one dogs were divided into a control group and 6 experimental groups. The experimental groups were adminstered orally 5, 10, 15, 30, 60 and 120mg of cadmium per kg of body weight for 56 days. All dogs were examined for clinical signs, and weekly changes in hematological and blood chemical values. All dogs were necropsied on 57th days of experiment. Tissue samples including hair, skin, muscle, lung, liver, kidney, spleen, pancreas, testis, ovary, uterus, and bone were collected and analyzed for cadmium, zinc, iron and copper contents using atomic absorption spectrophotometer. From these experiments following results were obtained : 1. All experimental dogs showed vomitting, salivation, anorexia, decreased water-intake, dehydration, and marked weight loss. The dogs received 30mg/kg or more of cadmium died during the period from 2nd to 7th week after administration. 2. Hematologically, all experimental dogs showed decrease in erythrocyte count, hemoglobin concentration, and packed cell volume. The anemia was identified as normocytic and regenerative morphologically. 3. No significant differences in serum glutamic oxaloacetic transminase, glutamic pyruvic transaminase, blood urea nitrogen, and cholosterol value were obseved between the control and experimental dogs. 4. The cadmium contents in various tissues of experimental dogs were estimated as $37.8{\sim}201.8{\mu}g/g$ in bone, $14.1{\sim}49.5{\mu}g/g$ in liver, $13.2{\sim}53.1{\mu}g/g$ in kidney, $0.4{\sim}35.2{\mu}g/g$ in pancreas, $0.8{\sim}35.4{\mu}g/g$ in spleen, $0.9{\sim}30.1{\mu}g/g$ in hair, $0{\sim}7.1{\mu}g/g$ in lung, $0{\sim}5.1{\mu}g/g$ in skin, and $0{\sim}3.6{\mu}g/g$ in muscle, respectively. However, the serum, testis, ovary and uterus showed no cadmium accumulation. Two contol dogs showed cadmium accumulation only in bone. 5. Significant differances in zinc, iron, and copper contents in tissue samples were observed between the control and experimental groups.

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Quality Characteristics of Mackerel Surimi Prepared by Alkaline Washing under Reduced Pressure (감압 알칼리 수세하여 제조한 고등어 Surimi의 품질 특성)

  • Park, Hyung-Sun;Park, Sang-Woo;Yang, Seung-Taek
    • Korean Journal of Food Science and Technology
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    • v.30 no.5
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    • pp.1120-1127
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    • 1998
  • An attempt was made in this study to investigate the optimum condition of washing for preparation of mackerel surimi by alkaline washing of 1, 3, 5, and 7 times under atmospheric (760), 660, and 560 mmHg pressure. The qualities of surimis were examined by analyzing the factors such as water content, crude lipid, pH, volatile basic nitrogen (VBN), expressible drip, protein extractability, $Mg^{2+}-$, $Ca^{2+}-$ and EDTA-ATPase activity, transglutaminase (TGase) activity, gel strength and color. The contents of moisture, crude lipid, pH and VBN in surimis prepared by alkaline washing under atmospheric, and reduced pressure went up to $72.0{\sim}72.9%$, $4.8{\sim}5.7%$, $6.9{\sim}7.0$ and $6.7{\sim}7.0\;mg/100\;g$, respectively. Protein extractability, ATPase activity and TGase activity were highest in surimis prepared by alkaline washing under 560 mmHg. Gel strengths of surimi setting gel and cooked gel from five times washing under 560 mmHg were 420 g cm (atmospheric, 330 g cm) and 485 g cm (atmospheric, 412 g cm), respectively. For the preparation of mackerel surimi, optimum washing condition was five times washing under 560 mmHg.

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Evaluation of Sanitation Management Practices and Microbiological Quality of Foods in Kindergarten Foodservice Settings (유치원 급식의 위생관리 실태조사 및 미생물적 품질평가)

  • Lee, Joo-Eun;Choi, Kyung-Sook;Kang, Young-Jae;Kwak, Tong-Kung
    • Korean journal of food and cookery science
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    • v.28 no.5
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    • pp.515-530
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    • 2012
  • This research aims to audit foodservice sanitation management practices and to assess microbiological quality of foods and their food contact environments in kindergartens. Sanitation auditing was conducted in 10 kindergartens in Seoul, Gyeonggi, and Incheon areas to assess the levels of safety practices. Results revealed that the surveyed kindergartens scored 41.4 out of 100 points, on average. The average scores of each category were 6.4/11 (58.1%) for facilities sanitation, 4.2/12 (35.0%) for equipment sanitation, 2.4/10 (24.0%) for personal hygiene, 5.1/10 (51.0%) for food ingredients management, 6.0/17 (35.3%) for production process, 5.4/10 (54.0%) for environmental sanitation, 2.0/6 (33.3%) for kitchen utensils sanitation, and 2.2/6 (96.7%) for safety management. Microbiological quality of raw, prepared foods, personal sanitation (hands), environmental sanitation, and drinking water were assessed. Total plate counts (TPC) of the following menus exceeded the critical limit: seasoned leek (5 log CFU/g), cucumber (5.0 log CFU/g), panbroiled fish paste (TNTC at $10^4$), tangpyeongchae (5.3 log CFU/g), egg rolls (6.1 log CFU/g), panbroiled sausage (TNTC at $10^4$), and soft tofu pot stew (TNTC at $10^4$). Coliform which exceeded the standard limit were detected from seasoned leek (2 log CFU/g), cucumber (2.5 log CFU/g), panbroiled fish paste (2.0 log CFU/g), egg roll (3.8 log CFU/g), tangpyeongchae (4.0 log CFU/g), panbroiled sausage (2.3 log CFU/g), and soft tofu pot stew (3.7 log CFU/g). For seasoned foods (muchim), S. aureus ranged 2.2~2.9 log CFU/g. In food workers' hands, microbial profiles ranged 3.8~7.9 log CFU/hand for TPC, ND~4.5 log CFU/hand for coliforms, ND~4.7 log CFU/hand for S. aureus, and ND~5.3 log CFU/hand for Enterobacteriaceae. Microbiological profiles of food contact surface of knives, cutting boards, dish-clothes, and trays showed possibilities of cross-contamination. General bacteria were 2.1~4.5 logCFU/ml in 4 purified water samples and E. coli were found in the kitchen of one kindergarten. These results suggested that environmental sanitation management practices need more strict improvement: effective sanitation education methods and practices were strongly required, and more strict sanitation management for cooking utensils and equipment were required.