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STUDY OF RAT EPIGASTRIC VESSELS ACCORDING TO THE FREEZING TIME : HISTOLOGIC, HISTOMORPHOMETRIC, IMMUNOHISTOCHEMICAL & SCANNING ELECTRON MICROSCOPIC STUDY (백서 상복부 혈관의 동결시간에 따른 변화에 대한 연구)

  • Kim, Woo-Chan;Lee, Chong-Heon;Kim, Kyung-Wook;Kim, Chang-Jin
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.21 no.2
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    • pp.89-109
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    • 1999
  • Vascular spasm which has been reported to occur in 25% of clinical cases continues to be a problem in microvascular surgery; When prolonged and not corrected, it can lead to low flow, thrombosis, and replant or free flap failure. Ischemia, intimal damage, acidosis and hypovolemia have been implicated as contributors to the vascular spasm. Although much work has been done on the etiology and prevention of vasospasm, a spasmolytic agent capable of firmly protecting against or reversing vasospasm has not been found. Therefore vascular freezing was introduced as a new safe method that immediately and permanently relieves the vasospasm and can be applied to microsurgical transfers. Cryosurgery can be defined as the deliberate destruction of diseased tissue or relief the vascular spasm in microvascular surgery by freezing in a controlled manner. 96 Sprague Dawley rats each weighing within 250g were used and divided into 2 group, experimental 1 and 2 group. In the experimental 1 group, right epigastric vessels (artery and vein) were freezed with a cryoprobe using $N_2O$ gas for 1 min. In the experimental 2 group, after freezing for 1 min, thawing for 30 secs and repeat freezing for 30 secs. Left side was chosen as control group in both group. We sacrified the experimental animals by 1 day, 3 days, 1 week, 2 weeks, 4 weeks & 5 months and observed the sequential change that occur during regeneration of epigastric vessels using a histologic, histomorphometric, immunohistochemical and SEM study after the vascular freezing. The results were as follows1. In epigastric arteries, internal diameters had statistically significant enlargement in 1 day, 3 days of Exp-1 group and 1 day, 3 days, 1 week & 2 weeks of Exp-2 group. Wall thickness had statistically significant thinning in 2 weeks of Exp-2 group. 2. In epigastric veins, internal diameters had enlargement of statistical significance in 1 day of Exp-1 and Exp-2 group. 3. The positive PCNA reactions in smooth muscle appeared in 1 week and increased until 2 weeks, decreased in 4 weeks. There was no statistical significance between Exp-1 and Exp-2 group. 4. The positive ${\alpha}$-SMA reaction in smooth muscles showed weak responses until 1 week and slowly increased in 2 weeks and showed almost control level in 4 weeks. 5. The positive S-100 reactions in the perivascular nerve bundles showed markedly decrease in 1 day, 3 days and increased after 1 week and showed almost control level in 4 weeks. Exp-1 group had stronger response than Exp-2 group. 6. In SEM, we observed defoliation of endothelial cell and flattening of vessel wall. Exp-2 group is more destroyed and healing was slower than Exp-1 group. To sum up, relief of vasospasm (vasodilatation) by freezing with cryoprobe was originated from the damage of smooth muscle layer and perivascular nerve bundle and the enlargement of internal diameter in vessels was similar to expeimental groups, but Exp-2 group had slower healing course and therefore vessel freezing in microsurgery can be clinically used, but repeat freezing time needs to be studied further.

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Development of instrument for measuring Home Economics-Pedagogical Content Knowledge(H-PCK) (가정교과교육학 지식(H-PCK)의 측정도구 개발)

  • Lee, Seung Jin;Yu, Nan Sook
    • Journal of Korean Home Economics Education Association
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    • v.29 no.1
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    • pp.35-56
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    • 2017
  • The purposes of this study were to develop an instrument to examine the latent domains to measure H-PCK and verify the reliability and validity of the instrument. To accomplish these purposes, instrument item development, content validity, pilot study, and main study were conducted. The results were as follows. First, based on a review of extant literature, 29 items for H-PCK were developed. Seven items were deleted from the original instrument after determining content validity by 10 in-service Home Economics teachers, which resulted in the 22 items of 3 domains(Knowledge of perspective on Home Economics Education(KP), Knowledge of Home Economics curriculum(KC), Knowledge of Home Economics instructional strategies(KI)). Second, data were collected from 137 Home Economics teachers via mail survey for pilot study to establish reliabilities for each individual domain and across the domains based on Cronbach's ${\alpha}$ and item-total correlation. The result showed good reliabilities in the cut-off value of .7 and .5 for Cronbach's ${\alpha}$ and for item-total correlation respectively. Third, the main study was performed with 220 Home Economics teachers via e-mail survey and the reliability and validity tests were conducted. The reliability test results showed good reliabilities. The model for confirmatory factor analysis(CFA) provided a good fit to the data (e.g., CFI=.92, RMSEA=.06, SRMR=.05) to evaluate construct validity. The three domains of KP, KC, and KI demonstrated the acceptable convergent and discriminant validities in each individual domain and over domains. Thus, the instrument in this study may be utilized to measure H-PCK. Finally, criterion-related validity was performed to examine the extent to which the three domains are related to teacher efficacy with Pearson correlation (${\rho}$). It was relatively highly correlated at ${\rho}=.7$. In addition, the higher H-PCK the Home Economics teachers had, the higher teacher efficacy they had. The final instrument consisting of 22 items from 3 domains were determined through the entire procedure.

Effect of Pimpinella Brachycarpa Ethanol Extract on Chronically Ethanol-Induced Liver Damage in Rats (참나물이 만성적으로 알코올 유도된 흰쥐의 간손상에 미치는 영향)

  • Choo, Myung-Hee;Lee, Jae-Joon;Lee, Myung-Yul
    • Journal of Life Science
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    • v.17 no.10
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    • pp.1406-1413
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    • 2007
  • This study was performed to investigate the effect of ethanol extract of Pimpinella brachycarpa(PBE) on chronically ethanol-induced hepatotoxicity in rat liver. Sprague-Dawley rats weighing 90-130 g were divided into 5 groups; normal group(NOR), ethanol(35%, 10 ml/kg) treated group(CON), PBE 200 mg/kg treated group(P1), PBE 200 mg/kg and ethanol treated group(P2), and PBE 400 mg/kg and ethanol treated group(P3). PBE was also fractionated by the following solvent: n-hexane, chloroform, ethylacetate and n-butanol. The antioxidative capacity of the n-hexane fraction was the highest among fractions and was similar to that of butylated hydroxytoluene(BHT). The body weight gain and feed intake of the rats were decreased by ethanol administration, but were gradually increased to the similar levels of the NOR group by administering PBE. The AST activity in serum elevated by ethanol was significantly decreased by administering the high dosage of PBE, but exerted no significant change on serum ALT activity. It was also observed that the hepatic activities of xanthine oxide(XO), catalase and glutathione peroxidase(GSH-Px) increased by ethanol were markedly decreased in the combined ethanol and PBE administered groups(P2 and P3), but not in the activity of superoxide dismutase(SOD) as compared with the CON group. The glutathione(GSH) contents were decreased by ethanol adminstration, however, increased after administering PBE. These results suggest that ethanol extract of Pimpinella brachycarpa has a possible positive effect on the liver function in hepatotoxicity-induced rats by ethanol administration.

Genesis and Classification of the Red-Yellow Soils derived from Residuum on Acidic and Intermediate Rocks -II. Songjeong series (산성암(酸性岩) 및 중성암(中性岩)의 잔적층(殘積層)에 발달(發達)한 적황색토(赤黃色土)의 생성(生成) 및 분류(分類) -제(第)II보(報) 송정통(松汀統)에 관(關)하여)

  • Um, Ki Tae
    • Korean Journal of Soil Science and Fertilizer
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    • v.6 no.2
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    • pp.75-81
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    • 1973
  • The morphological, physical, and chemical properties of Sonjeong series derived from acidic crystalline rocks are presented. Also it deals with the genesis and classification of the Songjeong series. Morphologically these soils have brown to dark brown loam A horizons and yellowish red to red clay loam Bt horizons with moderate, medium subangular blocky structure and thin patchy clay cutans on the ped faces. C horizons are very deep, yellowish red to yellowish brown fine sandy loam or sandy loam with original rock structure. Physically distribution of particle size indicates that clay increases with depth up to argillic horizons but below the argillic horizons clay content decrease. The moisture holding capacity is fairly good in Songjeong soils. Chemically soil reaction is strongly to very strongly acid throughout the profile and content of organic matter is less than 1 per cent except A horizons. Cation exchange capacity ranges from 5 to 9 me/100g of soils and base saturation is less than 35 per cent throughout the profile. The natural fertility of Songjeong soils are usually low. It needs lime, organic matter, and heavy application of fertilizer for the crop land. These soils occur temperate and humid climate under coniferous, deciduous, and mixed forest vegetation. Songjeong soils are classified as Red-Yellow Soils. Characteristically Songjeong soils are similar to Red-Yellow Podzolic soils in the United States but lack of A2 horizons and are quite liket Red-Yellow Soils of the Japan. According to new classification system which is 7th approximation of USDA Songjeong soils can be classified as fine loamy, mesic family of Typic Hapludults and in the FAO/UNESCO project World Soil Map as Orthic Acrisols.

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Morphometric Analyses with 15 Subspecies of Striped Field Mouse, Apodemus agrarius Pallas(Mammalia, Rodentia) from Eurasia (유라시아에서 서식하는 등줄쥐, Apodemus agrarius Pallas (포유 강,설치 목),15아종의 형태 형질의 분석)

  • Hung Sun Koh;G. Tikhonova
    • Animal Systematics, Evolution and Diversity
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    • v.14 no.4
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    • pp.341-355
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    • 1998
  • Thirty one morphometric characters of specimens in 15 subspecies of striped field mouse(Apodemus agrarius Pallas) from Eurasia were analyzed to clarify taxonomic status of these subspecies. Five major subgroups in A. agrarius were revealed: I, a largest-size form, specimens from two southern from other six localities in Korea, subspecies coreae and pallescens; III, the other large-size from, specimens from Astrachan in western Russia, subspecies volgensis; IV, a medium-size form, specimens from 16 localities in eastern Asia(North Korea, China, and eastern Russia), subspecies coreae, manchuricus, pallidior, ningpoensis, and insulaemus; V, a small-size form, specimens from 16 localities in western Asia and Europe (Kazakhstan, Russia, Lithuania, and Ukraine), subspecies tianschanicus, ognevi, agrarius, septentrionalis, nikolski, caucasicus, and karelicus. From this morphometric analyses, the followings are concluded: subspecies chejuensis is a larger-size form, as noted by Johnson and Jones(1955): subspecies pallescens is the synonym of subspecies agrarius, as suggested by Koh(1986): subspecies coreae from Korea is a large-size form and is idistinct from other 12 subspecies in Eurasia: the eastern form of subspecies ningpoensis by Corbet(1978) is a medium-size form o subspecies manchuricus, pallidior, ningpoensis, and insulaemus from eastern Asia (China and eastern Russia), and it includea North Korea specimens: a small-size form from western Asia and Europe(subspecies tianschanicus, ognevi, agrarius, septentrionalis, nikolski, caucasicus, and karelicus) is the western form of subspecies agrarius by Corbet(1978); the other large-size form of subspecies volgensis from western Russia is a distinct subspecies, which differs from the western subspecies agrarius. Therefore, it is concluded that 15 subspecies of A. agrarius can be classifed into five subspecies (chejuensis, coreae, ningpoensis, agrarius, and volgensis), although it is necessary to measure and analyze morphometric characters of specimens of other seven subspecies(albostriatus, maculatus, rubens, kahmanni, henrici, gloveri, and harti) for the complete reclassification of this species).

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Fresh-water Algae Occurred in Paddy Rice Fields I. Regional Distribution (논발생(發生) 담수조류(淡水藻類)에 관(關)한 연구(硏究) I. 지대별(地帶別) 발생분포(發生分布))

  • Lee, H.K.;Park, J.E.;Ryu, G.H.;Lee, J.O.;Park, Y.S.
    • Korean Journal of Weed Science
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    • v.12 no.2
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    • pp.158-165
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    • 1992
  • A survey on nation-wide distribution of fresh-water algae occurring in paddy rice fields was conducted in 1991. The algae which were collected from the whole nation were classified into a total of 54 genera including 14 genera in the blue-green algae. 29 genera in the green algae, 1 genus in the stoneworts. 3 genera in the euglenoids and 7 genera in the diatoms. The green algae and diatoms occurring in plain regions were diverse in terms of the number of genera distributed, whereas there was no regional difference in diversity of the blue-green algae and the euglenoids. Among the green algae, the suspended unicellular algae such as the genera, Chlamydomonas, Pandorina and Gonium, were widespread in plain regions, but the multicellular algae such as the genera, Spirogyra, Oedogonium, Ulothrix and Hydordictyon, were major in mountainous and attitudinal regions. The filamentous green algae such as the genera. Cladophora and Rhizoclonium, were dominant in reclaimed saline fields. The blue-green algae Oscillatoria spp. and the diatoms Navicula spp. were abundant in soil flakes.

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Effects of Tested Pack Containing Plant Extracts on Elasticity and Size of Women's Breasts (식물추출물 팩의 여성가슴 탄력증진과 크기증대에 관한 연구)

  • Choi, Yea-Hun;Park, Min-Kyung;Kim, Yong-Gyun;Lee, Sang-Mong;Son, Hong-Joo;Park, Hyean-Cheal;Kim, Sun-Tae;Choi, In-Soo;Kim, Keun-Ki
    • Journal of Life Science
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    • v.22 no.3
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    • pp.407-416
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    • 2012
  • In this study, we purified the extracts from the seeds and the roots of various plant species, including Q. acutissima, C. lanceolata, P. mirifica, P. bambusoides, and S. repens, and then investigated the effects of these extracts on cell growth and fat accumulation in adipocytes. We found that the extracts purified from Q. acutissima, C. lanceolata, P. mirifica, P. bambusoides, and S. repens more effectively increased the cell growth, as well as promoting the fat accumulation in adipocytes to a greater extent, than other extracts in vitro. Therefore, we made breast packs containing these effective extracts, and then investigated whether they were effective in enhancing the elasticity and volume of women's breasts. The measurements of breast elasticity and size revealed that the breast packs efficiently increased the elasticity and size of women's breasts. Furthermore, evaluation of the questionnaires related to usage of the breast packs indicated great satisfaction in terms of the lift, firmness, and elasticity of breasts. In conclusion, extracts purified from Q. acutissima, C. lanceolata, P. mirifica, P. bambusoides, and S. repens leading to cell growth and fat accumulation in adipocytes can effectively contribute to improving the elasticity and size of women's breasts.

Topographic Factors Computation in Island: A Comparison of Different Open Source GIS Programs (오픈소스 GIS 프로그램의 지형인자 계산 비교: 도서지역 경사도와 지형습윤지수 중심으로)

  • Lee, Bora;Lee, Ho-Sang;Lee, Gwang-Soo
    • Korean Journal of Remote Sensing
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    • v.37 no.5_1
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    • pp.903-916
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    • 2021
  • An area's topography refers to the shape of the earth's surface, described by its elevation, slope, and aspect, among other features. The topographical conditions determine energy flowsthat move water and energy from higher to lower elevations, such as how much solar energy will be received and how much wind or rain will affect it. Another common factor, the topographic wetness index (TWI), is a calculation in digital elevation models of the tendency to accumulate water per slope and unit area, and is one of the most widely referenced hydrologic topographic factors, which helps explain the location of forest vegetation. Analyses of topographical factors can be calculated using a geographic information system (GIS) program based on digital elevation model (DEM) data. Recently, a large number of free open source software (FOSS) GIS programs are available and developed for researchers, industries, and governments. FOSS GIS programs provide opportunitiesfor flexible algorithms customized forspecific user needs. The majority of biodiversity in island areas exists at about 20% higher elevations than in land ecosystems, playing an important role in ecological processes and therefore of high ecological value. However, island areas are vulnerable to disturbances and damage, such as through climate change, environmental pollution, development, and human intervention, and lacks systematic investigation due to geographical limitations (e.g. remoteness; difficulty to access). More than 4,000 of Korea's islands are within a few hours of its coast, and 88% are uninhabited, with 52% of them forested. The forest ecosystems of islands have fewer encounters with human interaction than on land, and therefore most of the topographical conditions are formed naturally and affected more directly by weather conditions or the environment. Therefore, the analysis of forest topography in island areas can be done more precisely than on its land counterparts, and therefore has become a major focus of attention in Korea. This study is focused on calculating the performance of different topographical factors using FOSS GIS programs. The test area is the island forests in Korea's south and the DEM of the target area was processed with GRASS GIS and SAGA GIS. The final slopes and TWI maps were produced as comparisons of the differences between topographic factor calculations of each respective FOSS GIS program. Finally, the merits of each FOSS GIS program used to calculate the topographic factors is discussed.

Distress and Associated Factors in Patients with Breast Cancer Surgery : A Cross-Sectional Study (유방암 수술환자의 디스트레스 및 연관인자 : 단면연구)

  • Lee, Sang-Shin;Rim, Hyo-Deog;Woo, Jungmin
    • Korean Journal of Psychosomatic Medicine
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    • v.26 no.2
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    • pp.77-85
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    • 2018
  • Objectives : This study aimed to investigate the level of distress using the distress thermometer (DT) and the factors associated with distress in postoperative breast cancer (BC) patients. Methods : DT and WHOQOL-BREF (World Health Organization Quality of Life Scale Abbreviated Version) along with sociodemographic variables were assessed in patients undergoing surgery for their first treatment of BC within one week postoperatively. The distress group consisted of participants with a DT score ${\geq}4$. The prevalence and associative factors of distress were examined by descriptive, univariable, and logistic regression analysis. Results : Three hundred seven women were recruited, and 264 subjects were finally analyzed. A total of 173 (65.5%) were classified into the distress group. The distress group showed significantly younger age (p=0.045), living without a spouse (p=0.032), and worse quality of life (QOL) as measured by overall QOL (p=0.009), general health (p=0.005), physical health domain (p<0.000), and psychological health domain (p=0.002). The logistic regression analysis showed that patients aged 40-49 years were more likely to experience distress than those aged ${\geq}60years$ (Odds ratios [OR]=2.992, 95% confidence interval [CI] 1.241-7.215). Moreover, the WHOQOL-BREF physical health domain was a predictive factor of distress (OR=0.777, 95% CI 0.692-0.873). Conclusions : A substantial proportion of patients are experiencing significant distress after BC surgery. It would be expected that distress management, especially in the middle-aged patients and in the domain of physical QOL (e.g., pain, insomnia, fatigue), from the early BC treatment stage might reduce chronic distress.

Evaluation of Nutritional Content and Quality Attributes of Cookies Utilizing Calcium-Enriched Finger Millet Variety (Finger1ho) (칼슘 함량이 높은 손가락조 '핑거1호'와 그 가공품의 영양 및 품질 특성)

  • Ji Ho Choo;Jee-Yeon Ko;Meyong Eun Choe;Ji Young Kim;Byong Won Lee;Young Kwang Ju;Hyoseob Seo;Choon-Song Kim;Sang-Ik Han
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.68 no.4
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    • pp.422-430
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    • 2023
  • The nutrient-rich and climate-resilient finger millet (Eleusine coracana (L.) Gaertn.) is a relatively new crop on the agricultural landscape. The present study explores the agronomic characteristics and antioxidant activities of grains and cookies produced from 'Finger1ho,' which was the first finger millet variety developed in South Korea. With heightened calcium content (314 mg/100 g) and polyphenol levels, 'Finger1ho' exhibited superior radical scavenging activities compared to other millets. The investigation assessed the impact of whole finger millet flour at varying concentrations (0, 10, 30, 50, and 100%) on cookie properties. Increasing the substitution of finger millet flour in the cookie formulation resulted in a notable rise in calcium content, ranging from 1.8 times at 10% to an impressive 10.8 times at 100%, surpassing the levels found in conventional wheat cookies. Conversely, the sodium (Na) levels in finger millet cookies demonstrated minimal variations, presenting a potentially favorable aspect in addressing the high Na intake prevalent in the South Korean diet. Notably, the antioxidant activity, assessed through ABTS and DPPH radical scavenging assays, exhibited a significant elevation compared to the control. This increase in antioxidant activity was directly proportional to the quantity of finger millet incorporated (p<0.001), indicating the potential health benefits associated with higher levels of finger millet in the cookie formulation. This study highlights finger millet's potential as a beneficial ingredient, enhancing both consumer acceptability and the functional attributes of cookies. Notably, cookies with 10% to 50% added finger millet exhibited significantly superior quality characteristics compared to controls, suggesting promising avenues for health-functional cookie development.