• Korean Journal of Environmental Agriculture
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• v.17 no.3
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• pp.220-226
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• 1998

The effects of metalaxyl(granule), carbofuran(granule) and simazine(water soluble powder) on the soil microflora were conducted at field soil between Iksan and Chonju province. Pesticides were divided into 0.5, 1, 1.5 and 2.0 times of normal of field, respectively. The number of fluorescent Pseudomonas was ranged from $10^3$ to $10^6/g$ in both field soil treated with cabofuran. Pseudomonas concentration of Chonju field soil slowly increased and approached the maximum level at 56 day after treatment(DAT). It showed the higher at 14DAT than other DAT in Iksan field soil treated with metalaxyl or simazine, whereas it increased again at 112 DAT in metalaxyl treatment. Cabofuran treatment of both field soil showed maximum Pseudomonas number at 28 DAT compared to that of other treatments. In Chonju field soil, those Pseudomonads of metalaxyl and simazine treatment increased the highest level at 7 DAT. Simazine treatment decreased it's number from the beginning of experiment. In both soil, metalaxyl treatment decrease the general fungi number at 7 DAT, but increase at 14 and 56 DAT in Iksan field soil. However it increased at 56 DAT in Chonju field soil. Cabofuran treatment of Iksan field soil tended to decrease general fungi number at 28 DAT, but was ranged from 1.0 to $8.6{\times}104/g$ for the rest of experimental period. It started to increase at 56 DAT simazine treatment of Iksan. General bacterial concentration both soil treated with cabofuran was belong to $26.6{\sim}29.6{\times}106$. It was the highest at 56 DAT, but was not significantly different. General actinomyces number was highly increased at 7 and 112 DAT compared to that of other DAT. Pseudomonas putida or P. fluorescens from both field soil was separated and identified 10 to 30 of all 104 Pseudomonas, respectively. All isolated microorganisms showed chemical resistance of 100ppm metalaxyl, cabofuran and simazine treatment.

• Korean Journal of Environmental Agriculture
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• v.17 no.3
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• pp.260-267
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• 1998

This experiment used the enclosed bench-scale reactors of 242 liters was conducted to obtain basic data on temporal and spatial variations in temperature, oxygen and moisture content, which were important factors of biological activities, during composting of mixture of dairy manure and rice straw. The reactors with thermocouples, oxygen sensor and datalogger were aerated at four different rates of 0.09, 0.18, 0.90 and 1.79 l $min^{-1}kg$ dry $solids^{-1}$. The higher aeration rates were, the faster the rates of increase and decrease in composting temperature were in both of initial and turnover stage, and the smaller the temperature difference between exhaust air and composting materials. Composting temperature of initial stage increased suddenly in all aeration rates, then stationary phase of temperature in materials and exhaust air showed at $50{\sim}53^{\circ}C$ for 5 hours and at $45^{\circ}C$ between 5 and 15 hours, respectively. In initial stage the maximum temperature was decreased with increasing aeration rates but in the stage after turnover it was the opposite except for 1.79 l $min^{-1}kg^{-1}$. Time arrived at the maximum temperature of composting materials was later in low-aeration rates than high-aeration rates at both stages. Time maintained high-temperature more than $45^{\circ}C$ was rapidly decreased with increasing aeration rates. In initial stage of composting maintaining time of $65^{\circ}C$ or more was the longest in the treatments of 0.09 and 0.18 l $min^{-1}kg{-1}$, while those of $55{\sim}65^{\circ}C$ and $45{\sim}55^{\circ}C$ was in 0.90 and 1.79 l $min^{-1}kg{-1}$, respectively. The minimum oxygen content and the maximum oxygen consumption rate in exhaust air through composting materials showed the increased trends with increasing aeration rates. In initial stage the minimum oxygen content was ranged from 0.9% to 7.4% for 32 to 59.5 hours and the maximum oxygen consumption rate was $1.89{\sim}6.48$ $gh^{-1}kgVS^{-1}$. In the stage after turnover their levels were $2.1{\sim}19.9%$ and $1.76{\sim}3.49 %$g/h-㎏ VS, respectively, for 16 to 49.5 hours.

• Journal of Food Hygiene and Safety
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• v.27 no.2
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• pp.169-175
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• 2012

Five kinds of selective media, such as mannitol salt agar (MSA), Baird-Parker agar (BPA), Baird-Parker supplemented with rabbit plasma fibrinogen (BPA+RPF), CHROMagar Staphylococcus aureus (CSA), and Petrifilm Staph Express count system (Petrifilm), were compared to recommend the optimum selective media for isolation of Staphylococcus aureus from agricultural products. Seventy four target and non target bacteria were inoculated on five selective media to analyze sensitivity and specificity. In the recovery test of injured S. aureus cells, S. aureus was exposed to acid (1% lactic acid for 10 min), heat ($60^{\circ}C$ for 90s), and cold ($-20^{\circ}C$ for 1h) conditions. And artificially contaminated agricultural products (iceberg lettuce, green pepper, and cherry tomato) was enumerated on five selective media. The sensitivity of BPA+RPF, CSA, Petrifilm, MSA, and BPA were 100%, 100%, 100%, 90.5%, 90.5%, respectively. In addition, the specificity of BPA+RPF, CSA, MSA, BPA and Petrifilm were 100%, 100%, 84.6%, 75.0%, 67.3%, respectively. However, no difference among five selective media was observed in recovery on injured S. aureus cell and enumeration from agricultural products. This results suggest that BPA+RPF and CSA are the optimum media for detection of S. aureus from agricultural products.

• Journal of The Korean Society of Grassland and Forage Science
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• v.23 no.1
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• pp.65-70
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• 2003

This study was conducted to select the promising varieties of alfalfa (va.) Venal, Ancho., P5444, DK120, DK125, Horizon, DK135, Luna and Kitawacaba at Daekwanryong branch(altitude 800m a.s.l.) and Namwon branch(altitude 450m a.5.1.) of National Livestock Research Institute Leaf width of alfalfa was broad in all varieties except fer Vernal, and DK125 was flowered the earliest of all varieties. Plant height of Luna was the longest, and winter hardness of P5444 was the greatest with 80.2% of all varieties. In Daekwanryong, dry matter(DM) yield of Luna was the highest and DM yield of vernal was the lowest among the varieties in Namwon. In both areas, dry matter yield ef P5444 was the highest of alfalfa varieties. In Daekwanryong, ADF and NDF concentrations of DK 120 were the lowest of all alfalfa varieties. In Namwon, ADF concentrations of Horizon was the lowest with 26％, and NDF concentrations of DK 125 and Horizon was the lowest with 40.8%. Crude protein concentrations of P5444, Luna and DK 120 were higher than that of different varieties in Daekwanryong and crude protein content of Horizon was the highest with 28.1% in Namwon. Average crude protein yield of both areas was the highest of P5444. The results of this study indicated that Luna, Horizon and P5444 would be the promising alfalfa varieties in Daekwanryong, Namwon and both areas, respectively.

• The Korean Journal of Pesticide Science
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• v.6 no.4
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• pp.250-256
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• 2002

The objective was to know if chinese cabbage growers in Gangwon alpine farmland control agricultural pests including weeds effectively and use pesticide properly. Examiners visited 185 farmers at Taebaek, Pyongchang, and Jeongseon and surveyed 33 questions on pest control methods pesticide use. Chinese cabbage farmers have noxious plant diseases such as clubroot, bacterial soft rot, downy mildew, anthracnose, and mosaic disease, and also noxious insects such as diamondback moth, aphid, beet armyworm, common cabbage worm, and Japanese native slug. In addition, farmers have noxious weeds such as common chickweed, marsh pepper, hairy crabgrass, common purslane, and horseweed. To control diseases and insects, 51.3% of farmers used many chemical agents, while 20.7% of farmers used chemical agents with too much emphasis on paraquat and glyphosate to control weeds: 87.2% of the answered farmers have a preference of the both non-selective herbicides. Farmers in the survey area selected pesticides on the basis of their own experience and sales managers' recommendation (84.2%) which results in the use of inappropriate pesticides such diniconazole. Many farmers have experienced phytotoxicities (46.7%) and pesticide poisoning (51.2%). We conclude that a systematic educational program for the proper selection and use of pesticides should be conducted for chinese cabbage growers in Gangwon alpine farmland.

• The Korean Journal of Pesticide Science
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• v.18 no.2
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• pp.79-87
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• 2014

Pyriofenone is an aryl phenyl ketone fungicide that is newly registered in Korea in 2013 to control powdery mildew on food. The objective of this study was to develop reliable and sensitive analytical method for determination of pyriofenone residue in agricultural products for ensuring the food safety. The pyriofenone residues in all samples(Korean melon, pepper, potato, mandarin, soybean, and hulled rice) were extracted with acetonitrile, partitioned with dichloromethane, and then purified with a silica cartridge. The purified samples were analyzed by HPLC-UVD and confirmed with LC-MS. The linear range of pyriofenone was 0.05~5 mg/kg with the correlation coefficient ($r^2$) > 0.999. Average recoveries of pyriofenone ranged from 72.8% to 99.5% at the spiked level of 0.05 and 0.5 mg/kg, while the relative standard deviation was 2.3%~6.4%. In addition, the limit of detection and limit of quantification were 0.01 and 0.05 mg/kg, respectively. The results revealed that the developed and validated analytical method was suitable for pyriofenone determination in agricultural products.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.7
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• pp.384-395
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• 2018

This study was conducted to investigate the distribution and variation of the anion and cation number of aerosols in the A and B regions of Gyorae forests. Ions were measured using an ion number meter between 28 June and 13 July 2017. The total average number of anions and cations were $735ions/cm^3$ and $459.27ions/cm^3$, respectively, which were measured at five sites in A area at average temperature of $27.81^{\circ}C$, wind speed of 0.28 m/sec, and altitude of 455.7 m. The average number of anions and cations were $780ions/cm^3$ and $379.55ions/cm^3$, respectively, which were measured at all four sites in the B region at average temperature of $27.6^{\circ}C$, humidity of 80%, wind speed of 0.1 m/sec and altitude of 477 m. The number of anions and cations in the A and B regions was $757.5ions/cm^3$ and $419.41ions/cm^3$, respectively. The number of ions was highly variable for each measurement over time. The number of anions remained $275.73ions/cm^3$ higher than that of cations. The variance of the measured values of anions and cations between and within sites A and B was significant (p<.001) and there was a significant positive correlation between regional mean values of anions and cations. In conclusion, the temporal distribution and variation of the ion content in the Gyorae forests provide basic information regarding aerosol compositions and changes.

• Clinical and Experimental Reproductive Medicine
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• v.30 no.2
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• pp.141-150
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• 2003

Objective: To evaluate whether diagnostic laparoscopy before transcervical fallopian tube catheterization (TFTC) would improve tubal recanalization rate and pregnancy rate in patients with bilateral proximal tubal blockage in hysterosalpingogram (HSG). Methods: The retrospective study was performed in those underwent TFTC from January 1998 to December 2001. A total of 50 patients with bilateral proximal tubal blockage in HSG were subjected to TFTC sequentially using repeated HSG (rHSG), selective salpingography (SS) followed by tubal catheterization (TFTC). Each procedure was terminated once patency had been achieved without proceding to the next technique. In Group A patients (n=35, 64 tubes), diagnostic laparoscopy was performed before TFTC was taken to exclude the tube combined with peritubal adhesion or distal tubal pathology. In Group B, patients (n=15, 26 tubes) were performed TFTC without diagnostic laparoscopy. Results: There were significant difference in clinical pregnancy rate (45.7% vs 15.4%, p=0.034) but no differences were found in recanalization rate (75.0% vs 73.1%) and complication rate (8.6% vs 13.3%). Although there is no signficant difference, more tubes were canalized by SS, which means tubal obstruction rather than occlusion, in Group A (25.0% vs 5.3%, p=0.069). Conclusion: Diagnostic laparoscopy would be effective in the selection of tube for the relatively inexpensive and less invasive TFTC or patients in need of assisted reproductive technologies. With the tubes without combined peritubal adhesion or distal tubal pathology, pregnancy rate was significantly increased.

• Journal of Yeungnam Medical Science
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• v.6 no.2
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• pp.173-181
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• 1989

The effect of interpregnancy interval on birthweight of the subsequent child was investigated for the 1,347 womens of 25 to 40 years old age who visited OBGY and Pediatric department of the general hospital in Taegu city. Questions in designed questionnaire were asked by student interviewers who were trained in nursing school. Mean birth weight by interpregnancy intervals were compared by the intervals of 6 months. Mean birth weight increased from 3,250 grams for intervals of 6 months to 3,357 grams for intervals of 25-30 months, hut the difference was not statistically significant(=0.47). Correlations between the continuous variables which were suspected as con founders and interpregnancy interval and birth weight were investigated. The coefficient of correlation between maternal age and interpregnancy interval was 0.39, between gestational period and birth weight 0.30 and between prepregnant weight and birth weight 0.16 and between birth weight of first baby and birth weight(of second baby) 0.44. But maternal age, gestational period and prepregnant weight were not considered as confounder, because they were not correlated simultaneously with birth interval and birth weight. Associations between the discrete variables which were suspected as confounders, and interpregnancy interval were investigated by Chi-square test. Associations between interpregnancy interval and educational level of mothers, types of husband's occupation, types of medical security, sex were not significant(P-values were 0.59, 0.75, 0.75, 0.82 respectively), so we did not considered these variables as confounding variables. In multiple regression analysis of birth weight, significant variables were birth weight of first baby, gestational period, sex of neonate and prepregnancy body weight of mother. Of the 1,347 births, the rate of low birth weight was 2% (27 birth). The rate for interpregnancy interval 7-12 months was highest as 3.6% and that for 13-18 months was lowest as 0.6%, but there was no regular tendency related with interpregnancy interval.

• Clinical and Experimental Pediatrics
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• v.49 no.1
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• pp.56-63
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• 2006

Purpose : This study was designed to document the etiologies and the characteristics of parapneumonic effusion in children. Methods : During a 17-year period from 1987 to 2004, parapneumonic effusion was confirmed in 86 children at Gyeongsang National University Hospital. The clinical records of these children were reviewed and radiological findings and laboratory data, especially results of thoracentesis, were analyzed retrospectively. Results : M. pneumoniae(34 subjects) was the most common pathogen at all over age, especially above 1-years-old. There were diagnosed with clinical characteristics and serologic tests. The $2^{nd}$ most common pathogen revealed non tuberculous bacteria(14 subjects). A species of bacteria at no tuberculous bacteria revealed S. aureus(5), S. pneumoniae(3), P. aeroginosa(3), other staphylococcus (2), and K. pneumoniae(1). There were confirmed with sputum culture or pleural fluid culture or blood culture. S. aureus was most common pathogen in infants. The $3^{rd}$ common pathogen was M. tuberculosis(7). There were confirmed with skin tuberculin tests and AFB stains. Another that was classified as a non bacteria was adenovirus(2). Complications of parapneumonic effusion such as pleural thickness occurred on M. tuberculosis(1). Non tuberculous bacteria, especially S. aureus revealed a serious predominance of polymorphocyte at pleural fluid, and lowest pleural pH and glucose, and highest pleural protein and LDH. Tuberculosis revealed high pleural protein and LDH. Conclusion : Age and chemistries of pleural fluid might be helpful in differentiating various etiologies of parapneumonic effusion. If there were suspicious of tuberculosis and non-tuberculous bacteria, more aggressive approaches were needed to prevent complication.