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http://dx.doi.org/10.13103/JFHS.2012.27.2.169

Evaluation of Selective Media for Isolation of Staphylococcus aureus from Agricultural Products  

Kim, Se-Ri (Microbial Safety Division, Department of Agro-Food Safety, NAAS, RDA)
Lee, Seo-Hyun (Microbial Safety Division, Department of Agro-Food Safety, NAAS, RDA)
Seo, Min-Kyoung (Microbial Safety Division, Department of Agro-Food Safety, NAAS, RDA)
Kim, Won-Il (Microbial Safety Division, Department of Agro-Food Safety, NAAS, RDA)
Park, Kyeong-Hun (Microbial Safety Division, Department of Agro-Food Safety, NAAS, RDA)
Yun, Hye-Jeong (Microbial Safety Division, Department of Agro-Food Safety, NAAS, RDA)
Yoon, Yo-Han (Department of Food &Nutrition, Sookmyung Women's University)
Yoo, Soon-Young (Seoul Regional Korea Food & Drug Administration)
Ryu, Kyoung-Yul (R&D Evaluation Division, Research Policy Bureau, RDA)
Yun, Jong-Chul (Microbial Safety Division, Department of Agro-Food Safety, NAAS, RDA)
Kim, Byung-Seok (Microbial Safety Division, Department of Agro-Food Safety, NAAS, RDA)
Publication Information
Journal of Food Hygiene and Safety / v.27, no.2, 2012 , pp. 169-175 More about this Journal
Abstract
Five kinds of selective media, such as mannitol salt agar (MSA), Baird-Parker agar (BPA), Baird-Parker supplemented with rabbit plasma fibrinogen (BPA+RPF), CHROMagar Staphylococcus aureus (CSA), and Petrifilm Staph Express count system (Petrifilm), were compared to recommend the optimum selective media for isolation of Staphylococcus aureus from agricultural products. Seventy four target and non target bacteria were inoculated on five selective media to analyze sensitivity and specificity. In the recovery test of injured S. aureus cells, S. aureus was exposed to acid (1% lactic acid for 10 min), heat ($60^{\circ}C$ for 90s), and cold ($-20^{\circ}C$ for 1h) conditions. And artificially contaminated agricultural products (iceberg lettuce, green pepper, and cherry tomato) was enumerated on five selective media. The sensitivity of BPA+RPF, CSA, Petrifilm, MSA, and BPA were 100%, 100%, 100%, 90.5%, 90.5%, respectively. In addition, the specificity of BPA+RPF, CSA, MSA, BPA and Petrifilm were 100%, 100%, 84.6%, 75.0%, 67.3%, respectively. However, no difference among five selective media was observed in recovery on injured S. aureus cell and enumeration from agricultural products. This results suggest that BPA+RPF and CSA are the optimum media for detection of S. aureus from agricultural products.
Keywords
Agricultural products; Staphylococcus aureus; selective media; sensitivity; specificity;
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