• Title/Summary/Keyword: 4D-Var

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A Phenolic Glucoside Isolated from Prunus serrulata var. spontanea and its Peroxynitrite Scavenging Activity

  • Jung Hyun Ah;Chung Hae Young;Kang Sam Sik;Hyun Sook Kyung;Kang Hye Sook;Choi Jae Sue
    • Archives of Pharmacal Research
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    • v.28 no.10
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    • pp.1127-1130
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    • 2005
  • A new phenolic glucoside (1), pursargentoside, was isolated from the leaves of Prunus serrulata var. spontanea, along with three other known compounds, orobol 7-O-glucoside (2), $1{\beta}$, $2{\alpha}$, $3{\alpha}$, 24-tetrahydroxy-urs-12-en-28-oic acid (3), and chlorogenic acid (4). The structure of pursargentoside (1) was identified by spectroscopic data analysis including 1D and 2D NMR spectroscopy, as 2-O-${\beta}$-(6'-benzoyl)-glucopyranosyl o-(Z)-coumaric acid. Compounds 1, 2, and 4 exhibited ONOO- scavenging activity, whereas compound 3 was determined to be virtually inactive.

Kluyveromyces marxianus var. marxianus IFO 1735에 의한 Inulin Fructotransferase의 생산 및 이용에 관한 연구

  • 김재근;판정척부
    • Microbiology and Biotechnology Letters
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    • v.25 no.3
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    • pp.277-285
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    • 1997
  • Kluyveromyces marxianus var. marxianus isolated as an inulin-assimilating microorganism produces inulin fructotransferase (inulaseII) which catalyses the conversion of inulin into di-D-fructofuranose 1, 2' : 2, 3' dianhydrde (DFAIII). The DFA produced by the organism was isolated by using active carbon column, and identified as DFAIII by high performance liguid chromatography. The culture medium giving maximum inulaseII production was found to consist of 1% sucrose and 0.75% yeast nitrogen base (YNB). The inulasell production was induced by inulin or sucrose as a carbon source and increased by addition of YNB as a nitrogen source. Optimal initial pH of the culture medium, culture temperature and medium volume for the enzyme production were pH 4.7, 30$\circ$C and 140 ml, respectively. Under the optimal conditions described above, the enzyme activity in the culture supematant reached 4.2 units/ml after cultivation for 36 h. The DFAIII was accumulated at 13.25 mg/ml after 48 h of culture in the Jerusalem artichoke tuber medium.

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The Inhibitory Principle of Lipopolysaccharide-Induced Nitric Oxide Production from Inula Britannica var. Chinensis

  • Je, Kang-Hoon;Han, Ah-Reum;Lee, Hyun-Tai;Mar, Woong-Chon;Seo, Eun-Kyoung
    • Archives of Pharmacal Research
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    • v.27 no.1
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    • pp.83-85
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    • 2004
  • A sesquiterpene lactone, 1-O-acetyl-4R,6S-britannilactone (1) isolated from the flowers of Inula britannica L. var. chinensis (Rupr.) Reg. (Compositae), was found as an iNOS inhibitory constituent for the first time with an $IC_{50}$ value of 22.1 $\mu$ M which is more potent than the positive control, L-$N^6$-(1-iminoethyl)lysine ($IC_{50} =33.7 \mu$ M). Structure of compound 1 was identified by 1D and 2D NMR experiments and by comparison with the reference standard.

Triterpenoidal Saponins from the Bark of Kalopanax pictum var. typicum

  • Cho, Soon-Hyun;Hahn, Dug-Ryong
    • Archives of Pharmacal Research
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    • v.14 no.1
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    • pp.19-24
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    • 1991
  • One new triterpenoidal saponin, saponin F(2) has been isolated from the bark of Kalopanax pictum Nakai var. typicum (Araliaceae), together with one known saponin, kizuta saponin $K_{12}$ (1). On the basis of chemico-spectral evidences, the structure of 2 has been elucidated to be 3-O-${\beta}$-D-xylopyranosyl$(1{\rightarrow}3)$-${\alpha}$-L-rhamnopyranosyl$(1{\rightarrow}2)$-${\alpha}$-L-arabinopyranosyl-23-hydroxyolean-12-en-28-O-${\alpha}$-L-rhamnopyranosyl$(1{\rightarrow}4)$-${\beta}$-D-glucopyranosyl$(1{\rightarrow}6)$-${\beta}$-D-glucopyranosyl ester.

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Phenolic Compounds on the Leaves of Betula Platyphylla var. latifolia

  • Lee, Min-Won;Takashi Tanaka;Gen-Ichiro Nonaka;Hahn, Dug-Ryoung
    • Archives of Pharmacal Research
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    • v.15 no.3
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    • pp.211-214
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    • 1992
  • Chemical examination of Betula platyphylla var. latifolia afforded a novel diarylheptanoid named betulateraol, together with a phenylpropanoid (3, 4'-dihydroxypropio-phenone), flavan-3-ol [(+)-catechin] and its glycosides [(+)-catechin 5-O-$\beta$-glucopyranoside, (+)-catechin 7-0-$\beta$-D-glucopyranoside] and two proanthocyanidins (procyanidins B-1 and B-3).

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Aromatic Compounds and Tritepenoidal Saponins from Clematis koreana var. umbrosa

  • Whang, Wan-Kyunn
    • Archives of Pharmacal Research
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    • v.17 no.1
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    • pp.5-10
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    • 1994
  • From the methanolic extract of aerial parts of Clematis koreana var. umbrosa, one new triterpenoidal saponin, 3-O-${\beta}$-D-xylopyranosyl(1-3)-${\alpha}$-L-arabinopyranosyl oleanolic acid 28-O-${\alpha}$-L-rhamnopyranosyl(1-4)-${\beta}$-glucopyranosyl(1-6)-${\beta}$-glucopyranosyl ESTER, along with five known aromatic compounds and two known triterpenoidal saponins were isolated.

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Developmental Distribution on Fungi in Mt.Jiri Areas (1. On unrecorded species in Fungi) (지리산의 균류의 발생분포에 관한 연구(I) (1. 균류의 미기록종을 중심으로))

    • Korean Journal of Plant Resources
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    • v.12 no.1
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    • pp.62-68
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    • 1999
  • Many fungi were collected at Mt.Jiri national park from April 1,1996 to March 30,1998. They were identified. According to the results, following species newly to Korea ; in eumycota Omphalina ericetorum, Cortinarius traganus, Russula polyphylla, Coltricia montagnei var. montagnei, Cantharellus ferruginascens, Dacrymyces capitate, Arachnopeziza nivea. Dasyschyphus pygmeaus, tenuissimus, Nectria punicea var. punicea and in myxomycota Trichia affnis, Physarum viride var. aurantium. These unrecorded species were designed Korean common name by author.

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Chloroplast genome of the conserved Aster altaicus var. uchiyamae B2015-0044 as genetic barcode

  • Lee, Minjee;Yi, Jae-Sun;Park, Jihye;Lee, Jungho
    • Journal of Species Research
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    • v.10 no.2
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    • pp.154-158
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    • 2021
  • An endemic endangered species, Aster altaicus var. uchiyamae (Danyang aster) B2015-0044, is cultivated at the Shingu Botanical Garden, which serves as the ex situ conservation institution for this species. In this work, we sequenced the chloroplast genome of A. altaicus var. uchiyamae B2015-0044. We found that the chloroplast (cp) genome of B2015-0044 was 152,457 base pairs(bps) in size: 84,247 bps of large single copy regions(LSC), 25,007 bps of inverted repeats(IRs), and 18,196 bps of small single copy regions. The B2015-0044 cp genome contains 79 protein-coding genes (PCGs), 4 RNA genes, 29 tRNA genes, and 3 pseudogenes. These results were identical to a previously reported cp genome (Park et al., 2017), except for two sites in introns and three in intergenic spacer (IGS) regions. For the intronic differences, we found that clpP.i1 had a 1-bp small simple repeat (SSR) (T) and petD.i had a 3-bp SSR (ATT). We found 1-bp SSRs in the IGSs of trnT_ggu~psbD and psbZ~trnG_gcc, C and A, respectively. The IGS of(ndhF)~rpl32 had a SNP. Based on our results, the cp genome of the A. altaicus var. uchiyamae can be classified into two genotypes, [C]1-[A]12-[T]12-[ATT]4-C and [C]2-[A]11-[T]11-[ATT]2-A.

Host Preference of Gray Field Slug, Deroceras reticulatum Müller, and Its Development and Longevity on Host Plants (작은뾰족민달팽이의 기주 선호성 및 기주에 따른 발육과 수명)

  • Kim, Hyun-Ju;Bae, Soon-Do;Yoon, Young-Nam;Choi, Byeong-Ryeol;Park, Chung-Gyoo
    • Korean journal of applied entomology
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    • v.51 no.1
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    • pp.33-37
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    • 2012
  • This study was conducted to investigate food preference and developmental characteristics of the gray field slug, $Deroceras$ $reticulatum$ M$\ddot{u}$ller (Stylommatophora: Limacidae) using various foods. The food preference of $D.$ $reticulatum$ was most highest on Chinese cabbage ($Brassica$ $campestris$ L., followed on cucumber ($Cucumis$ $sativus$ L.), and significantly low on beet ($Beta$ $vulgaris$ var. Ruba), kale($Brassica$ $oleracea$ L. var. $acephala$ D.C), carrot (Daucus carota L.) and Chinese cabbage ($Brassica$ $campestris$ L.) which were not different significantly. The total number of eggs laid by $D.$ $reticulatum$ was the highest (n = 109.6) on cucumber and the lowest (n = 10.1) on leaf perilla ($Perilla$ $frutescens$ var. $japonica$ Hara). Hatchability was the highest (92.4%) on cucumber and the lowest (62.5%) on leaf perilla. The egg developmental period was not significantly different from 12 days to 13 days among host plants. Juvenile period and adult longevity ranged from 75 days to 111 days and 66 days to 187 days, respectively. Thereby, life span from egg to adult longevity of $D.$ $reticulatum$ was the longest on cucumber with 273 days and the shortest on 190 days on leaf perilla.

A Comparison the Volatile Aroma Compounds between Ligularia fischeri and Ligularia fischeri var. spiciformis Leaves (곰취와 한대리곰취의 휘발성 향기성분 분석)

  • Han, Sang-Sup;Sa, Jou-Young;Lee, Kyeong-Cheol
    • Journal of Forest and Environmental Science
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    • v.26 no.3
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    • pp.209-217
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    • 2010
  • The volatile aroma of fresh leaves is one of main factor in taste of all the edible green plants. The volatile aroma in almost edible green leaves are suggested as essential oil compounds. Ligularia fischeri, Synurus deltoides, Ligularia fischeri var. spiciformis and Aster scaber are one of the favourable edible green plants in Korea. In this study, volatile aroma compounds from Ligularia fischeri and Ligularia fischeri var. spiciformis species were analyzed by the SPME/GC/MSD method. Ligularia fischeri had 78 volatile aroma compounds such as D-limonene(20.28%), ${\alpha}$-pinene(dextro, 14.15%), L-${\beta}$-pinene(12.85%), 3-carene, ${\beta}$-cubebene(10.39%), etc. Ligularia fischeri var. spiciformis had 83 volatile aroma compounds such as D-limonene(36.97%), ${\beta}$-cubebene(13.95%), L-${\beta}$-pinene(13.38%), ${\alpha}$-pinene(dextro, 4.76%), caryophylle-ne(3.33%) etc. Conclusively, the commom volatile aroma compounds in Ligularia fischeri and Ligularia fischeri var. spiciformis leaves were D-limonene, ${\alpha}$-pinene, L-${\beta}$-pinene, ${\beta}$-cubebene, Caryophyllene, ${\alpha}$-farnesene, terpinolen. However, the composition and amount of volatile aroma compounds were very different between the two species.