Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Kluyveromyces marxianus var. marxianus IFO 1735에 의한 Inulin Fructotransferase의 생산 및 이용에 관한 연구

  • 김재근 (계명전문대학교 식품영양과 Tel. 82-53-620-2639 E-mail : lsx＠kw.keimgung-c.ac.kr) ;
  • 판정척부 (대판부립대학 농학부 응용생물화학과)
  • Published : 1997.06.01
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Abstract

Kluyveromyces marxianus var. marxianus isolated as an inulin-assimilating microorganism produces inulin fructotransferase (inulaseII) which catalyses the conversion of inulin into di-D-fructofuranose 1, 2' : 2, 3' dianhydrde (DFAIII). The DFA produced by the organism was isolated by using active carbon column, and identified as DFAIII by high performance liguid chromatography. The culture medium giving maximum inulaseII production was found to consist of 1% sucrose and 0.75% yeast nitrogen base (YNB). The inulasell production was induced by inulin or sucrose as a carbon source and increased by addition of YNB as a nitrogen source. Optimal initial pH of the culture medium, culture temperature and medium volume for the enzyme production were pH 4.7, 30$\circ$C and 140 ml, respectively. Under the optimal conditions described above, the enzyme activity in the culture supematant reached 4.2 units/ml after cultivation for 36 h. The DFAIII was accumulated at 13.25 mg/ml after 48 h of culture in the Jerusalem artichoke tuber medium.

Keywords

References

  1. Polysaccharide Chemistry v.27 Whister, R. L.;C. L. Smart
  2. Biochem. J. v.48 The carbohydrates of the Jerusale martichake and other compositae Bacon, J. S. D.;J. Edelman
  3. New phytol. v.67 The mechanism of fructosan metabolism in higher plants as examplified in Helinathus tuberosus Edelman, J.;T. G. Jefford
  4. Kor. J. Appl. Microbiol. Bioeng. v.12 Ethanol production from Jerusalem artichoke tubers (Helianthus tuberosus L) by Kluyveromyces fragilis Ryu, Y. W.;C. H. Kim;S. I. Kim
  5. J. Kor. Agric. Chem. Soc. v.26 Selection of yeast strains for alcohol production from Jerusalem artichoke tubers (Helianthus tuberosus L.) Ryu, Y. W.;C. H. Kim;S. I. Kim
  6. Kor. J. Appl. Microbiol. Biotechnol. v.21 no.1 Screening of the endoinulase-producing fungi by using antibody Lee, S. H.;M. K. Kim;M. S. Chung;Y. S. Jeong;T. B. Uhm
  7. 식품기능화학 최동성;고하영
  8. Kor. J. Biotechnol. Bioeng. v.5 Ethanol fermentation characteristics of K. marxianus on Jerusalem artichoke tuber extract Kim, J. H.;B. K. Hur;C. S. Bae;H. S. Kim
  9. Kor. J. Appl. Microbiol. Biotechnol. v.22 no.6 Screening and identification of wild stains for the production of high concentration of alcohol from Jerusalem artichoke Tubers Hong, Y.;E. H. Choi
  10. Fructans in the biochemistry of plants v.14 Pollock, C. J.;N. J. Chatternon
  11. Fructans in higher plants in polysaccharide chemistry Whistler, R. L.;C. L. Wmart
  12. Agric. Biol. Chem. v.47 no.2 Formation of di-D-fructose anhydride from inulin by the root of Lycoris radiata Herbert Uchiyama, T.
  13. J. Ferment. Bioeng. v.72 no.4 Production of inulin fructotransferase (Depolymerizing) by Arthrobacter sp. H65-7 and preparation of DFA Ⅲ from inulin by the enzyme Yokota, A.;S. Hirayama;K. Enomoto;Y. Miura;S. Takao;F. Tomita
  14. Kor. J. Appl. Microbiol. Biotechnol. v.20 no.3 Growth effect of branched oligosaccarides on principal intestinal bacteria Park, J. H.;J. Y. Yoo;O. H. Shin;H. K. Shin;S. J. Lee;K. H. Park
  15. Agric. Biol. Chem. v.53 no.8 Purification and properties of a novel inulin fructotransferase (DFAI-producing) from Arthrobacter globiformis S14-3 Seki, K.;K. Haraguchi;M. Kishimot;S. Kobayashi;K. Kainuma
  16. Agric. Biol. Chem. v.53 no.3 On the enzyme of Aspergillus fumigatus producing difructose anhydride I from inulobiose Matsuyama, T.;K. Tanaka
  17. J. Biochem. v.92 Enzyme formation of di-D-fructose 1,2' : 2, 1' dianhydride from inulobiose by Aspergillus fumigatus Matsuyama, T.;K. Tanaka;M. Mashiko;M. Kamamoto
  18. Carbohydrate Res. v.75 Enzyme formation of di-D-fructose 1, 2' : 2, 1' dianhydride by Aspergillus fumigatus Tanaka, K. K. Sonobe;T. Uchiyama;T. Matsuyama
  19. Biochem. Biophys. Acta v.284 Formation of di-D-fructofuranose 1, 2' : 2, 3' dianhydride from inulin by an extracellular inulase of Arthrobacter ureafaciens Tanaka, K.;T. Uchiyama;A. Ito
  20. Agric. Biol. Chem. v.52 no.1 Purification and properties of inulin fructotransferase (depolymerizing) from Arthrobacter globiformis C 11-1 Haraguchi, K.;M. Kishimoto;K. Seki;K. Hayashi;S. Kobayashi;K. Kainuma
  21. Agric. Biol. Chem. v.52 no.1 Purification and some properties of inulin fructotransferase (depolymerizing) from Arthrobacter ilicis Kawamura, M.;S. Takcahashi;T. Uchiyama
  22. Agric. Biol. Chem. v.55 no.5 Isolation and identification of the Aspergillus fumigatus difructose dianhydride Matsuyama, T.;K. Tanaka;T. Uchiyama
  23. J. Ferment. Technol. v.52 no.3 Preparation of Di-D-fructofuranose 1, 2' : 2, 3' dianhydride by the cultivation of Arthrobacter ureafaciens in the inulin-containing synthetic and vegetable media Shirasawa, E.;T. Uchiyama;K. Tanaka
  24. Kor. J. Appl. Microbiol. Biotechnol. v.21 no.1 Production of Inulin fructotransferase (Depolymerizing) from Enterobacter sp. S45 Kang, S. I.;S. I. Kim
  25. Biochem. Biophys. Acta v.315 Purification and properties of Arthrobacter ureafaciens Inulase Ⅱ Uchiyama, T.;S. Niwa;K. Tanaka
  26. J. Biol. Chem. v.179 A photometric method to the determination of inulin in plasma and urine Roe, J. H.;J. H. Epstein;H. P. Goldstein
  27. Anal. Chem. v.31 Use of dinitrosalicylic acid reagent for determinati- on of reducing sugar Miller, G. L.
  28. J. Biol. Chem. v.193 Protein measurement with the folin phenol reagent Lowry, O. H.;N. J. Rosenbrough;A. L. Farr;R. J. Randall