• Korean Journal of Pharmacognosy
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• v.16 no.4
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• pp.233-238
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• 1985

A number of sedative alkaloids were isolated from Sanjoin(酸棗仁), the seeds of Zizyphus vulgaris Lamark var. spinosus Bunge (Rhamnaceae) which is an important Chinese medicinal material used to treat insomnia and sometimes to treat sleepiness. Those compounds were designated as Sanjoinine-A, B, C, D, etc. depending on the order of increasing polarity. Sanjoinine-A, $C_{31}H_{42}N_4O_4$, $mp\;249^{\circ}$, $[{\alpha}]^{27}_D-316$, Sanjoinine-B, $C_{30}H_{40}N_4O_4$, $mp\;212{\sim}4^{\circ}$, Sanjoinene, $C_{29}H_{35}N_3O_4$, $mp\;281{\sim}2^{\circ}$, $[{\alpha}]^{22}_D-272$, Sanjoinine-D, $C_{32}H_{46}N_4O_5$, $mp\;256{\sim}8^{\circ}$, $[{\alpha}]^{22}_D-53.6$, Sanjoinine-F, $C_{31}H_{42}N_4O_5$, $mp\;228{\sim}9^{\circ}$, $[{\alpha}]^{22}_D-215$, and $Sanjoinine-G_1,\;C_{31}H_{44}N_4O_5,\;mp\;236{\sim}8^{\circ},\;[{\alpha}]^{22}_D-68.6$, were found as 14-membered cyclic peptide alkaloids, $Sanjoinine-G_2,\;C_{30}H_{42}N_4O_4,\;mp\;182^{\circ},\;[{\alpha}]^{22}_D-79.2$, as being open chain peptide alkaloid, and Sanjoinine-E, $C_{19}H_{21}NO_2$, $mp\;166^{\circ}$, $[{\alpha}]^{20}_D-146.2$, N-Methylasimilobine, $C_{18}H_{19}NO_2$, $mp\;193{\sim}5^{\circ}$, $[{\alpha}]^{20}_D-204$, Sanjoinine-Ia, $C_{18}H_{19}NO_2$, $mp\;155{\sim}7^{\circ}$, $[{\alpha}]^{20}_D-140$, Sanjoinine-Ib, $C_{19}H_{21}NO_4$, $mp\;184^{\circ}$, Sanjoinine-K, $C_{16}H_{19}NO_3$, $mp\;159{\sim}61^{\circ}$, $[{\alpha}]^{20}_D+35$, Caaverine, $C_{17}H_{17}NO_2$, $mp\;204^{\circ}$, $[{\alpha}]^{20}_D-80$, and Zizyphusine, $C_{20}H_{24}NO_4$, $mp\;214{\sim}6^{\circ}$, $[{\alpha}]^{20}_D+317$ as being aporphine alkaloids. The heat treatment of the cyclic peptide alkaloids produced their isomeric products which showed enhanced sedative activity. The chemical structure of the isomeric products will be discussed.

• The Korean Journal of Ecology
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• v.15 no.3
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• pp.247-255
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• 1992

In order to estimate effects of air pollution for herbs in the plnus thunbergii forests arrounding onsan industrial complex, this study was surveyed. number of species of herb also had a difference over 10 species by distance at the source of air pollution, and species diversity and evennes were also increasing at remote sites in industrial complex as woody plants. At the vicinity of industrial complex, $SDR_4$ of calamagrostis arundinatios arundinacea., miscanthus sinensis and cymbopogon tortilis var. goeringii were very high, and those of lianas as cocculus trilobus, lonicera japonica and paederia scandens, and phytolacca americana were low, but because of being higher than other sites, it was able to inferred that lianas had a tolerance to air pollution. The composition of biological spectra of herbs showed to type of $H-D_1-R_{2-3}-1$ at near site in the source of air pollution and $H-D_1-R_{1-3}-t$ at the other sites, and the propotion of lians forms were relatively high at the vicinity of industril complex.

• Journal of the Korean Society of Food Science and Nutrition
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• v.34 no.5
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• pp.613-618
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• 2005

This study was carried out to examine the effects of gamma irradiation on the cytotoxicity and multidrug-resistance reversing activity of methanol extracts from Coix lachryma-jobi L. var. me-yuen Stapf seed. The seed was irradiated with doses of 1, 4, 8, 16, 32 and 64 Gy of the gamma radiation, and then extracted by methanol. The extracts were examined for cytotoxicity on the human cancer cell lines, MCF-7 (human breast adenocarcinoma pleural effusion), Calu-6 (human pulmonary carcinoma) and SNU-601 (human gastric carcinoma) cells, and investigated for multidrug-resistance reversing activity using drug sensitive AML-2/WT and multidrug-resistant AML-2/D100 cells. The growth inhibitory activity of irradiated seed extracts on human cancer cell lines was higher than that of the control. In the case of Calu-6 cell line, the effect of cytotoxicity was observed in the extracts of 4, 8 and 16 Gy. $IC_{50}$ value in the MCF-7 cell line was measured in the only 8 Gy extract. And in the SNU-601 cell line as Calu-6, the effect of cytotoxicity was observed in the extracts of 4, 8 and 16 Gy. But the extracts of gamma-irradiated seed over 32 Gy showed little growth inhibitory effect against human cancer cell lines. In this result, 8 Gy extract had significant growth inhibitory in all human cancer cell lines $(Calu-6:\;633\;{\mu}g/mL,\;MCF-7:\;653\;{\mu}g/mL\;and\;SNU-601:\;683\;{\mu}g/mL)$. The extracts of 4, 8 and 16 Gy strongly potentiated vincristine cytotoxicity in AML-2/D100 cells. The reversal fold (RF) of 4, 8 and 16 Gy extracts was 1.7, 1.8 and 1.6, respectively. But their cytotoxicities to both sensitive AML-2/WT and resistant AML-2/D100 cells were in the same order of magnitude. These results indicate that the above samples would contain some principles which have cytotoxicity and multidrug-resistance reversing activity. Irradiation technology can be applied to promote physiological activities of medicinal plant seeds.

• Journal of Life Science
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• v.8 no.5
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• pp.532-536
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• 1998

This study was designed to observe the effects of the feeding Gentiana scabra Bunge var.buergeri Max. extract on the improvement of the blood glucose, lipids in the serum of dietary hyperlipidemic rats (S.D. strain, ♂) fed the experimental diets for 4 weeks. Concentration of blood glucose was significantly higher in the cholesterol administration groups (group 2(cholesterol+water), 3(cholesterol+Gentiana scabra Bunge extract) than those in the control group(group 1 (water)). Blood glucose concentration was rather lower in the group 3(cholesterol+Gentiana scabra Bunge extract) than in the group 2(cholesterol+water). There was almost little significance among the groups. Concentration of total cholesterol in serum was significantly lower in the group 3 than those in the group 2. Concentration of LDL-cholesterol in serum was significantly lower in the group 3 than that in the group 2. In the ratio of HDL-cholesterol concentration to total cholesterol concentration, Gentiana scabra Bunge extract administration group was higher percentage than in the group 2. Concentration of free cholesterol and cholesteryl ester in serum was significantly lower in the group 3 than in the group 2. Concentrations of triglyceride and phospholipid in serum were decreased in the group 3 than in the high fat diet group. The activities of aspartate aminotransferase and alanine aminotransferase in serum were increased in the all experimental groups than in the control group. From the above research, the Gentiana scabra Bunge var.buergeri Max. exacts were effective on the improvement of the lipid composition in serum of dietary hyperlipidemic rats.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.12 no.6
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• pp.86-98
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• 2009

This study was carried out to investigate vegetation structure and soil properties of Adenophora racemosa population distributed in Jeombongsan, Seoraksan, Odaesan and Dutasan in Gangwon-do, Korea. From August 2007 until September 2009, $2m{\times}2m$ quadrate was established in native area of Adenophora racemosa in order to record a dominants and coverage, and soil factors at 22 sites. It was found that the altitude in the distributed areas for Angelica gigas population was 800 m or more. Adenophora racemosa population was classified into Rhododendron mucronulatum dominant population, Geranium var. hirsutum dominant population, Caret siderosticta dominant population and Aruncus dioicus var. kamtschaticus dominant population. In the site of study, soil pH, electrical conductivity, soil organic matter, available phosphorous, and exchangeable potassium, exchangeable calcium, exchangeable magnesium, exchangeable sodium concentration and total nitrogen were ranged from 5.0~7.1, 0.06~0.65dS/m, 0.96~8.94%, 12.3~32.8mg/kg, 0.12~0.89cmol+/kg, 0.34~10.08cmol+/kg, 0.1~l.4cmol+/kg, 0.02~0.29cmol+/kg and 0~8.4% respectively. According to the results of DCCA, Adenophora racemosa population were distributed in the high available phosphorous and exchangeable potassium, and Rhododendron mucronulatum dominant population was situated on low available potassium and high slope degree, Geranium var. hirsutum dominant population was high altitude, electrical conductivity and exchange magnesium, and Carex siderosticta dominant population and Aruncus dioicus var. kamtschaticus were distributed on high total nitrogen, organic matter, available phosphorous.

• Korean Journal of Plant Taxonomy
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• v.35 no.2
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• pp.129-142
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• 2005

Somatic chromosome numbers of 19 taxa of Korean Rubus was investigated. Subg. Anoplobatus (2 species), subg. Cylactis (1 species), subg. Idaeobatus (15 taxa) and subg. Malachobatus (1 species) are found in Korea. All taxa belonging to subg. Idaeobatus except for R. parvifolius which shows tetrapolid and hexaploid are diploid. The basic chromosome number of the genus was x=7. New chromosome numbers for 5 taxa were reported here: R. hongnoensis of Jeju-island endemic species, 2n=14; R. longisepalus, 2n=14; R. longisepalus var. tozawai, 2n=14; R. parvifolius, 2n=28; R. parvifolius var. taquetii, 2n=28. The rest 12 taxa except for R. coreanus Miq was well counted as 2n=14 and well consistent with previous reports from China and Japan. Our new chromosome level for R. parvifolius as 6x may indicate that speciation by polyploidization has occurred within Korean population. Unlikely to Japanese population (2n=42), Korean population of R. buergeri has same ploidy level with Taiwanese population as 2n=56.

• The Korean Journal of Ecology
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• v.26 no.3
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• pp.109-114
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• 2003

To investigate ecophysiological characteristics of plants species adapted to moor habitat, we selected 22 species plants and analyzed inorganic cations (K, Ca, Mg), heavy metals (Al, Fe, Mn) and total nitrogen and phosphorus quantitatively. Moojechi moor indicated typical acidic and oligotrophic conditions with pH of 5.0∼5.6 (pH 4.3∼5.1 in soil) and EC of 15∼30μ S/cm, and contained very low contents of soil divalent cation such as Ca and Mg but high contents of heavy metals (esp. Al). With respect to inorganic cation contents, investigated plants species showed remarkable interspecific difference. Plant species belonging to J. effusus var. decipiens, M. japonica, I. globosa, M. sacchariflorus, R. mucronulatum, R. yedoense var. poukhanense, H. micrantha, D. rotundifolia showed very low contents of inorganic cation below 400 μ M/g DW, but plant species of C. palustris var. spontanea, L. sessilifolia, P. mandarinorum, C. lineare, S. austriaca sub. glabra, V. mandshurica, A. decursiva showed high cation contents in leaves. Especially, S. austriaca sub. glabra (Compositae) and V. mandshurica (Violaceae) showed pattern accumulating Ca and Mg with plant growth, but I. ensata var. spontanea (Iridaceae) and S. officinalis (Rosaceae) showed decreasing tendency. Meanwhile, most plant species showed low contents of soluble metal ions in leaves in spite of high heavy metal contents on soil, and indicated remarkable interspecific differences in the total contents and composition of heavy metals accumulated. Despite low contents of N and P on soil, most plant species indicated relatively high contents of N and P in leaves at the early stage of growth, and showed slowly decreasing pattern according to growth. Consequently, it seems that plant species inhabited on Moojechi moor cope with acidic-oligotrophic conditions, accumulating inorganic cations and nitrogen at the early growing stage and reutilizing them in the course of growth, and developing heavy metal excluding mechanism.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.25 no.4
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• pp.1-13
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• 2022

This study was conducted to investigate the chlorophyll content, photosynthetic characteristics, and growth characteristics of Aster koraiensis and Dendranthema zawadskii var. latiloba according to shading treatment. A. koraiensis and D. zawadskii grew in four different shading treatment plots (0%, 50%, 75% and 95% of full sunlight) for experiments. It was found that as the shading level increased, the total chlorophyll content increased and dark respiration decreased in both A. koraiensis and D. zawadskii, indicating that A. koraiensis and D. zawadskii increase the utilization efficiency for photosynthesis under low light conditions. In addition, as the shading level increased, the net apparent quantum yield increased, resulting in the highest in the 95% shading plot, but the highest photosynthetic rate, water use efficiency, and leaf mass per area (LMA) were shown in the control group than in the shading treatments. The results showed that A. koraiensis and D. zawadskii are heliophytes showing plasticity to light, and if the light is restricted to continue to shade, it may be detrimental to growth. For healthy growth, it is considered suitable to grow A. koraiensis under full light conditions, and D. zawadskii under the light condition that blocks 0-50% of full sunlight.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.4
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• pp.504-511
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• 1995

This study was conducted to investigate the superoxide dismutase (SOD) characteristics and antioxidant activity by nonenzymatic(Fe$^{2+}$/Ascorbate) and Fe$^{3+}$-ADP/NADPH method in perilla(Perilla frutescens var. japonica Hara.) and jaso(Perilla frutescens Briton var. acuta Kudo.) leaves. The characteristics were evaluated by the nitro blue tetrazolium reduction method. Perilla leaves contained three or four major SODs depending on the varieties. The inhibitor test indicated that the Perilla leaves contained two Cu /ZnSODs and one or two FeSODs, but Jaso leaves have only Cu/ZnSOD. However, no varietal differences were detected in the Cu /ZnSOD isozyme patterns. FeSODs, however, showed different varietal isozyme patterns through the different combinations of the two FeSOD isozymes. Among MeOH extractes, "mil yang 2" showed very strong antioxidant activity. Relatively large differences in the levels of SOD and antioxidant activity detected in the Perilla varietites. There was significantly different in the comparison between perilla leaves and red jaso leaves.s.etween perilla leaves and red jaso leaves.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.40 no.1
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• pp.109-120
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• 2014

In this study, the extracts of Inula britannica var. chinensis (I. britannica) flower were extracted at three different temperatures (room temperature, $45^{\circ}C$, and $65^{\circ}C$) and their anti-aging effects were studied. Before investigating anti-aging effects of the extracts, their cytotoxicity was tested on B16F10, Hs683, and HaCaT cells. All extracts showed no cytotoxicity at the concentration less than 0.1% (v/v). Melanin synthesis inhibitory activities in B16F10 cells and reverse transcriptase polymerase chain reaction (RT-PCR) in Hs683 and HaCaT cells were used to see their anti-aging effects. The room temperature extract at 0.1% showed 24.5% melanin synthesis inhibition, which was better than the $45^{\circ}C$ and $65^{\circ}C$ extracts. In addition, expression rates of the room temperature extract at 0.1% on HAS-1, HAS-2, and HAS-3 related to hyaluronan synthase genes were 123.3%, 137.8%, 133.2%, respectively. which were higher than reference material of L-ascorbic acid. Expression rates of the $45^{\circ}C$ extract at 0.1% on TNF-${\alpha}$, COX-2, and IL-$1{\alpha}$, which are inflammatory related genes, was suppressed to 30.3%, 12.8%, 25.7%, respectively. It was better in anti-in flammatory effect than the room temperature and $65^{\circ}C$ extracts. As results, we showed that I. britannica var. chinensis flower extarcts decreased melanin production and expression of inflammatory related genes and increased the expression rate of hyaluronan synthase genes. Thus, it is believed that the extracts affect anti-aging effects of skin through whitening, moisturizing, and anti-inflammatory processes and could be applicable to cosmetics as a functional cosmetic ingredient.