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Resistance of Chili Pepper Cultivars to Isolates of Phytophthora capsici (Phytophthora capsici 균주들에 대한 고추 품종들의 저항성)

  • Jo, Su-Jung;Shim, Sun-Ah;Jang, Kyoung Soo;Choi, Yong Ho;Kim, Jin-Cheol;Choi, Gyung Ja
    • Horticultural Science & Technology
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    • v.32 no.1
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    • pp.66-76
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    • 2014
  • Resistance of one hundred commercialized cultivars of chili pepper to four isolates of Phytophthora capsici was evaluated under controlled environmental conditions. The cultivars are commercialized as resistant (59%) and susceptible (41%) to Phytophthora blight in Korea. Mean disease severities of the cultivars on P. capsici MY-1, KPC-1, JHAI1-7, and KPC-7 isolates were 37, 55, 60, and 74%, respectively. In addition, 38 for MY-1, 48 for KPC-1, 56 for JHAI1-7, and 76 cultivars for KPC-7 showed susceptibility. To P. capsici MY-1, the weakest pathogenicity isolate among them, 59 cultivars represented high resistance. By contrast, only six cultivars showed high resistance to P. capsici KPC-7, the strongest isolate. Furthermore, resistance of most cultivars except for three cultivars was negatively correlated with the virulence of P. capsici isolates. And isolate-specific resistance of the chili pepper cultivars could not be found. Among them, six cultivars showing resistance to all the tested isolates were selected for further study. The development of Phytophthora blight on the six cultivars according to inoculum density ($5{\times}10^4$ to $1.5{\times}10^6$ sporangia/pot) and incubation temperature (25 to $30^{\circ}C$) after inoculation of P. capsici was tested. Resistance of the cultivars to P. capsici KPC-1 and JHAI1-7, moderately pathogenic isolates, was hardly affected. But to KPC-7 isolate, the highly resistant cultivars showed susceptiblility or moderate resistance when the seedlings were inoculated with inoculum density of $1.5{\times}10^6$ sporangia/pot and incubated at 28 to $30^{\circ}C$. From these results, it is likely that resistance of chili pepper cultivars to Phytophthora blight is affected by the virulence of P. capsici isolate.

Effect of pH Control, Ozonation and Coagulation on THMs Formation in Dringking Water Treatment Process of the Downstream of Nakdong River (낙동강 하류의 정수처리 공정에서 pH, 오존 및 응집이 트리할로메탄 생성에 미치는 영향)

  • Lee, Jeong-Kyu;Son, Hee-Jong;Kim, Sang-Goo;Hwang, Young-Do;Ryu, Dong-Choon
    • Journal of Korean Society of Environmental Engineers
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    • v.39 no.3
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    • pp.105-111
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    • 2017
  • This study was conducted to evaluate the effects of pH control and ozonation, coagulation on trihalomethanes (THMs) formation during prechlorination of the Nakdong river water. The results showed that lower pH was reduced THMs formation during prechlorination. THMs formation of water lowered pH 9.5 to 9.0, was reduced 18.3% and lowered pH 9.0 to 8.0 was reduced 14%, lowered pH 8.0 to 7.0 was reduced 7%, lowered pH 9.5 to 8.0 was reduced 29%. A low ozone dose ($0.11{\sim}0.48mg{\cdot}O_3/DOC$) before chlorination reduced the yields of THMs (reduced 6~24% in chlorination) compared with no preozonation. Thus the low ozone dose pretreatment is relatively effective plan to reduce THMs formation during chlorination. When ozone 1.0 mg/L, Alum 40 mg/L and sulfuric acid 6 mg/L dosed, The yields of THMs formation was reduced 42% compared with only chlorination. Input of chlorine after preozonation (followed coagulation, pH control) is more effective than only decline pH at a intake station to control THMs formation in a water treatment process. When chlorine 2.5 mg/L was added before coagulation (alum 40 mg/L), THMs formation was reduced 19% by lower pH and decreased 18% by a natural organic matter (NOM) removal compared with only chlorine 2.5 mg/L addition. Because coagulation could induce simultaneously lower pH and NOM removal, THM formation concentration is more effectively lowed than decreasing pH in the Nakdong river water.

Study on Resource Plants of the Mt. Geonji, Jeonju City (전주시 건지산 일대의 자원식물상 연구)

  • Oh, Hyun-Kyung;Beon, Mu-Sup;Lim, Seong-Gu;Park, Joon-Moh;Kim, Kae-Hwan
    • Korean Journal of Plant Resources
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    • v.21 no.1
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    • pp.73-82
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    • 2008
  • The resource plants of the Mt. Geonji was listed 354 taxa; 92 families, 242 genera, 303 species, 48 varieties and 3 forms. 354 taxa listed consists of 205 taxa of edible plants(57.1%),234 taxa of medicinal plants(65.2%), 167 taxa of ornamental plants(46.5%) and 218 taxa of the others(60.7%). Specific plant species by floral region were total 22 taxa; Trapella sinensis var. antennifera in class IV, Iris ensata var. spontanea in Class II, 16 taxa(Salix glandulosa, Alnus hirsuta, Chrysosplenium flagelliferum, Mallotus japonicus, Ilex macropoda, Grewia biloba var. parviflora, Vaccinium oldhami, Lysimachia barystachys, Fraxinus mandshurica, etc.) in class I. The naturalized plants in this site were 12 families, 23 genera, 28 species, 2 varieties, 30 taxa(Bromus unioloides, Phytolacca americana, Oenothera erythrosepala, Ipomoea hederacea var. hederacea, Aster pilosus, Erechtites hieracifolia) and naturalization rate was 8.5% of all 354 taxa vascular plants. Wild plants disturbing ecosystem like Solanum carolinense and Ambrosia artemisiifolia var. elatior have been increasing. So, it needs continuing control and conservation measures on the plant ecosystem.

Effects of Tree Density Control on Carbon Dynamics in Young Pinus densiflora stands (소나무 유령림의 임목밀도 조절이 탄소 동태에 미치는 영향)

  • Song, Su-Jin;Jang, Kyoung-Soo;Hwang, In-Chae;An, Ki-Wan;Lee, Kye-Han
    • Journal of Korean Society of Forest Science
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    • v.105 no.3
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    • pp.275-283
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    • 2016
  • The objective of this study was to examine carbon dynamics with biomass, soil $CO_2$ efflux, litter and root decomposition after tree density control in young Pinus densiflora stands. The stands were established with 50% thinning, clear-cut, and control stands with three pseudo-replicated plots and a bare soil plot in 8-year-old Pinus densiflora nursery field. Monthly measurements were conducted from March 2012 to February 2014 and aboveground biomass and coarse-roots were estimated by derived allometric equations. Average diameter growth at root collar in control and thinned was 0.89 cm and 1.48 cm per year, respectively, and the diameter growth of control stand was significantly higher than that of thinned stands (p<0.05). Total biomass was estimated to 5.17, $4.85kg\;C\;m^{-2}$ per year in control and thinned, respectively. Annual soil $CO_2$ efflux in control, thinned, clear cut, and bare soil was 3.71, 3.90, 4.17, $4.56kg\;CO_2\;m^{-2}\;yr^{-1}$, respectively and removing trees significantly increased soil $CO_2$ efflux (p<0.05). Net Ecosystem Production (NEP) was 1.57, 1.36, -0.67, $-1.25kg\;C\;m^{-2}\;yr^{-1}$ in control, thinned, clear cut and bare soil in the young Pinus densiflora stands. NEP was significantly decreased by removing trees. Thinning increased diameter at root collar and carbon of individual tree and recovered 86% of carbon removed by thinning after one-year. In addition, soil $CO_2$ efflux increased and NEP increased by thinning. Results of this study, tree density control such as thinning increased the carbon storage and growth of the young Pinus densiflora stands.

Permeation Efficiency of Sea Tangle (Laminaria japonica) Extract into Egg Using Temperature Change Method and Pressure (온도 변화 방법과 압력을 이용한 계란 내부로 다시마 추출물의 침투 효율)

  • Park, Yeo-Jin;Kim, Dong-Ho;Jang, Keum-Il
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.4
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    • pp.544-549
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    • 2014
  • In this study, the permeation efficiency (PE) of sea tangle (Laminaria japonica) extract (STE) into egg using the temperature change method (TCM) and pressure was investigated. STE was prepared in a volume of water equal to 10 times the weight of the sea tangle at $100^{\circ}C$ over a 3 hr period. The PE of iodine as a representative component of the STE as well as the total polyphenol content at ambient pressure were determined as follows: 1) after soaking in the STE at 30, 40, and $50^{\circ}C$ for 3 hr, and 2) after soaking in the STE at $4^{\circ}C$ immediately after soaking in distilled water at $50^{\circ}C$ for 1, 2, and 3 hr (TCM), respectively. The PE of iodine as well as the total polyphenol content improved as the temperature of the STE increased (P<0.05). The permeation of iodine was more rapid, and the total polyphenol content increased with the TCM (121 ${\mu}g/100$ mg and 1.74 mg/mL for 3 hr, respectively) compared to without the TCM (72 ${\mu}g/100$ mg and 1.48 mg/mL for 3 hr, respectively), and the highest PE was observed with the TCM after soaking at $50^{\circ}C$ for 3 hr. At a pressure of 5.0 MPa, the PE was more rapid with the TCM (139 ${\mu}g/100$ mg and 1.74 mg/mL for 90 min, respectively) than without the TCM (105 ${\mu}g/100$ mg and 1.56 mg/mL for 90 min, respectively). Consequentially, these results suggest that the preparation of seasonings and functional eggs using various components and bioactive substances is possible by the TCM.

Evaluation of Perception and Foodservice Satisfaction of Free School Meals by Elementary School Students in Busan (부산지역 일부 초등학생의 무상급식에 대한 인식 및 급식품질 만족도)

  • Jang, Eun Ryung;Choi, Hee Sun;Lyu, Eun Soon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.45 no.12
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    • pp.1830-1837
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    • 2016
  • In this research, the perception and foodservice quality satisfaction of free school meals were surveyed by elementary school students in Busan. The survey was conducted on 600 elementary students of 4~6th graders. The total score for perception of free school meals was 4.47/5.00 points. Sixth graders showed a statistically significant higher score than fourth graders (P<0.01). The overall score for satisfaction of free school meals was 4.55 points. In terms of grade, sixth graders showed a statistically significant higher score than fifth graders (P<0.05). For satisfaction of foodservice quality, average score was 4.55 points. In terms of lower-level categories, areas of food, menu, sanitation, service, and environment yielded 4.47, 4.62, 4.62, 4.53, and 4.48 points, respectively. In terms of differences by grade, sixth graders showed a statistically significant higher average score for satisfaction of foodservice quality than fourth and fifth graders (P<0.01). The overall score for perception of free school meals and overall satisfaction of foodservice quality showed a statistically significant positive correlation (P<0.01, r=0.781). In each category of foodservice quality, areas of food (r=0.733), menu (r=0.677), sanitation (r=0.636), service (r=0.673), and environment (r=0.588) showed statistically significant positive correlations (P<0.01).

Development of an Efficient Screening System for Resistance of Watermelon Plants to Didymella bryoniae (수박 덩굴마름병에 대한 효율적인 저항성 검정 방법 개발)

  • Lee, Ji Hyun;Jang, Kyoung Soo;Choi, Yong Ho;Kim, Jin-Cheol;Choi, Gyung Ja
    • Research in Plant Disease
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    • v.22 no.2
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    • pp.72-80
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    • 2016
  • Gummy stem blight, caused by the fungus Didymella bryoniae, is major disease of watermelons worldwide. The objective of the present study was to establish an efficient screening system to identify watermelon resistant to D. bryoniae. An GSB3 isolate was prepared from a watermelon plant showing typical symptoms of gummy stem blight in Haman-gun and identified as D. bryoniae based on molecular analysis of internal transcribed spacer sequence. A simple mass-production technique of inoculum was developed based on spore production of D. bryoniae GSB3 under several incubation conditions and their virulence on watermelon plants. Resistance degrees of 22 commercial watermelon cultivars to the GSB3 isolate were evaluated. Among them, four watermelon cultivars showing different degree of resistance response were selected for further study. Development of disease on the cultivars according to various conditions including inoculum concentrations, incubation periods in dew chamber, and incubation temperatures was investigated. From the results, we suggest an efficient screening method for resistant watermelon cultivars to gummy stem blight. Seeds of watermelon cultivar are sown and grown in a greenhouse until plant stage of 2-fully expanded leaves. Seedlings are inoculated with D. bryoniae by spraying spore suspension of the fungus at a concentration of $5.0{\times}10^5spores/ml$. The infected plants are incubated in humidity chamber at $25^{\circ}C$ for 48 hours and then transferred to a growth chamber at $25^{\circ}C$ and 80% relative humidity with 12-hour light a day. Three to four days after inoculation, disease severity of the plant are measured using percentage of infected leaf area.

Variations of Agronomic Characteristics of Collected Coix lachryma-jobi Species (국내 율무 수집종의 특성변이)

  • Yi, Eun-Seob;Kim, Hee-Dong;Kim, Sung-Kee;Yoon, Seong-Tak
    • Korean Journal of Medicinal Crop Science
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    • v.12 no.4
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    • pp.279-284
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    • 2004
  • This experiment was conducted to evaluate on agronomic traits of Korean local adlay (Coix lachryma L.) germplasm, 281 cultivars (lines). The largest collected cultivars was from Kyungsangbukdo (56 cultivars), and the next was in the order of Cheonlabukdo (48 cultivars), Kyungsangnamdo (44 cultivars), Kyunggido (39 cultivars) Chungcheongnamdo (23 cultivars), Kangwondo (23 cultivars), Cheonlanamdo (17 cultivars), and Chungcheongbukdo (16 cultivars) in distribution of collected region for 281 cultivars. In the growth stages, days to emergence from seeding were required $16.4{\pm}2.03$ days, days to heading from emergence was required $73.6{\pm}5.87$ days, days to ripening from heading was required $52.3{\pm}4.91$ days and cultivation period was required $142.5{\pm}5.87$ days. Culm length was $210.3{\pm}16.39$ cm, no. of tiller per plant was $10.4{\pm}2.13$, culm diameter was $11.7{\pm}1.09$ mm, fruiting position was $4.6{\pm}0.82$ node, no. of main culm node was $11.1{\pm}0.78$, degree of lodging was $5.3{\pm}3.42$ and severity of leaf blight was $56.1{\pm}0.78$ in the growth characteristics of the germplasm. In the yield of component, no. of grains per $m^2$ was $5,938{\pm}2,152$, percentage of ripening was $67.7{\pm}33.12$, 1,000 grains weight was $123.8{\pm}33.76$ g and grain weight was $473.0{\pm}207.90$ g. Grain weight was correlates significantly positively with no. of main culm node, percentage of ripening and no. of grains per $m^2$ among agronomic traits. Correlation coefficient between grain weight and 1,000 grain weight was positive, while grain weight and severity of leaf blight was significantly negative. Correlation coefficient between grain weight and days to ripening from heading was significant negatively.

Histopathological Changes in Cold-Stored Dog Lungs to the Preservation Solutions (냉한에서 보관된 황견의 폐에서 장기 보존액에 따른 조직 세포의 변화)

  • 김해균;이두연
    • Journal of Chest Surgery
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    • v.29 no.8
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    • pp.816-821
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    • 1996
  • Lung transplantation is the established treatment for the end stage lung disedse find preservation of the organ is a major obstacle In performing lung transplantation. For solving this problem, we evaluated the histopathologic changes for various preservation solutions. Male mongrel dogs of similar size and weight (15∼20 kg) were used. The dog lungs were flushed with 4fl normal saline(group 1 'n:5): Modified Euro-Collins solution(group 2 n:5) and University of Wisconsin solution (group 3 : n=6), 60m11kg through a catheter placed in the main pulmonary artery aft r flushing of PGE 1 (20ng1kg). The lungs were preserved for 60 hours and measured dry and wet weights. Histologic specimens were taken every 6 hours and %toed for light microscopic evaluation. The edema ratio of the lungs peaked in 12 hours although there was no difference between the groups. Histologically, alveolar septal changes developed in one case (20%) after 1 hour preservation with normal saline. In case of the University of Wisconsin solution, the alveolar septal distortions and swellings were seen in 1 cases (20%) after 6 hours preservation compared with 3 cases (60%) after 6 hours preservation with Modified Euro-Collins solution. Changes of the pneumocytes were observed after 24 hours preser- vation in group 1, after 48 hours preservation in group 2 and after 60 hours preservation in group 3. We conclude that University of Wisconsin solution might have a superior preservation effect compare to normal saline and Modified Euro-Collins solutions.

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Effects of Cryosurgery in Primary Lung Cancer (원발성 폐암에서 냉동수술의 치료 효과)

  • Jung, Won-Jae;Kim, Kwang-Taik;Yi, Eun-Jue;Lee, Sung-Ho;Kang, Moon-Chul;Chung, Jae-Ho;Ham, Soo-Yeoun;Jo, Sung-Bum
    • Journal of Chest Surgery
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    • v.42 no.2
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    • pp.201-205
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    • 2009
  • Background: Cryosurgery has been used to treat primary malignant pulmonary tumors at our institute since November 2004. In this study we analyzed our treatment results and complication rates. Material and Method: A retrospective study using medical charts and imaging data was conducted involving 17 patients with a total of 17 malignant pulmonary tumors who were treated between November 2004 and March 2007. Fourteen patients were males and 3 were females. The median age of the patients was 64 years (range, $54{\sim}77$ years). The average size of the tumors was 48.8mm (range, $36{\sim}111mm$) in diameter. The patients were followed with chest CT scans 7 days, 1 month, 3 months, and 6 months postoperatively. PET scans were obtained between 6 and 9 months postoperatively. The treatment response was analyzed according to the Response Evaluation Criteria in Solid Tumors (RECIST) criteria. Result: Six months after treatment, 6 tumors (35.3%) showed a complete response, 4 (23.5%) had a partial response, 3 (17.6%) had stable disease, and 4 (23.5%) showed disease progression. In tumors <4 cm in diameter, a complete response was reported in 50% of the tumors. A $x^2$-test showed that in tumors <4 cm in diameter, the p-value for results better than a partial response was 0.034. With respect to procedural complications, there was 1 case of blood-tinged sputum which resolved spontaneously within 1 or 2 days, a spontaneously relieved case of subcutaneous emphysema, and 1 patient with a fever. There were no mortalities and the average hospital stay was 6.3 days. Conclusion: The effects of cryosurgery on primary lung cancer is greatest in patients with small tumors. Considering the facts that cryosurgery is minimally invasive, has a low complication rate, and can be performed repetitively, we believe that it may play an important role in the treatment of high risk lung cancer patients.