• Journal of Aquaculture
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• v.2 no.1
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• pp.9-20
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• 1989

This study was taken to isolate transferrin fractions from the sera of tilapia(Oreochromis niloticus) by physico-chemical analyses such as the rivanol precipitation, iron-staining method, SDS-polyacrylamide gel electrophoresis and $^{59}FeCl_3$ autoradiography, and to calculate gene frequencies by using Hardy-Weinberg Law. The results obtained in this experiment were summarized as follow : 1. The transferrin fraction is composed of several components possessing relative lower electrophoretic mobilities and higher molecular sizes than the albumin components. 2. When different staining method was compared with transferrin in band, it was not found difference. 3. It was concluded that the optimun ratio of rivanol to serum was 2 : 1 and this ratio was used in all further fractionation. 4. The molecular weight of transferrin component was about 70,000 $\pm$ 2,000. 5. Tilapia transferrin fractionations were found to be polymorphic. 6. There transferrin variants(A, B and C) have been found in tilapia(Oreochromis niloticus) and Tf types were assumed to be controlled by three codominant alleles Tf A, Tf B and Tf C. Six different phenotypes can be theoretically expected Tf AA, Tf AB, Tf AC, Tf BB, Tf BC, and Tf CC. Only five types of these were observed and Tf CC types(homozygotes) was not found. 7. The frequencies of the three allele Tf A, Tf B and Tf C were 0.795, 0.15 and 0.055 respectively.

• Korean Journal of Agricultural Science
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• v.22 no.1
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• pp.69-81
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• 1995

This study was conducted to examine the genetic variants of the blood proteins and enzymes in beef cattle breeds, Hereford, Angus and Sharolais reared at the Daekwanryuong Branch of the National Livestock Research Institute. Genetic polymorphisms of transferrin(Tf), post-transferrin2(pTf-2), albumin(Alb), post-albumin (pAlb), ceruloplasmin(Cp), amylase-I(Am-I) and hemoglobin(Hb) in blood were analyzed by the methods of PAGE(polyacrylamide gel electrophoresis) and STAGE(starch gel electrophoresis). The results obtained from this study were summarized as follows: 1. Tf and pTf-2 locus assumed to be controlled by codominant alleles, A. $D_1$, $D_2$ and E allele for Tf, F and S allele for pTf-2. In genotype frequencies, 25% and 90% for Tf $D_1D_2$ and pTf-2 SS in Hereford, 25% and 100% for Tf $AD_1$ and pTf-2 FF in Angus, 50% for Tf $D_1D_1$ and pTf-2 FS in Sharolais were found to have the highest frequency, respectively. In gene frequencies, 0.400 and 0.900 for Tf E and pTf-2 S allele in Hereford, 0.678 and 0.607 for Tf $D_1$ and pTf-2S in Sharolais were appeared to have the highest frequency. 2. Alb and pAlb locus assumed to be controlled by codominant alleles, only A allele for Alb, F and S allele for pAlb. In genotype frequencies, 70% for pAlb SS in Hereford, 90% for pAlb FF in Angus and 57.15% for pAlb SS in Sharolais were found to have the highest frequency. In gene frequencies, 0.825 and 0.750 for pAlb S in Hereford and Charolais, 0.900 for pAlb F in Angus were found to have the highest frequency. 3. Cp and Am-I locus appeared to be controlled by two alleles, F and S allele for Cp, B and C allele for Am-I. In genotype frequencies, 100% and 65% for Cp FF and Am-I BB in Hereford, 45% and 85% for Cp FF, and Am-I CC in Angus, 50% and 64.29% for Cp FF and Am-I BC in Sharolais were found to have the highest frequency. Gene frequencies were 1,000, 0.600 and 0.750 for Cp F in Herehord, Angus and Sharolais, 0.800, 0.875 and 0.680 for Am-I B, C and C allele in Hereford, Angus and sharolais, respectively. 4. Hb locus assumed to be controlled by codominant alleles, only A allele in Hereford and Angus, A and B allele in Sharolais. Genotype frequencies were 57.14% and 42.86% for Hb AA and AB in Sharolais, and gene frequencies were 0.785 and 0.215 for Hb A and B in Sharolais.

• Korean Journal of Food Science and Technology
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• v.46 no.4
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• pp.425-431
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• 2014

In this study, the volatile compounds in 24 commercial Korean black raspberry wines were isolated by headspace solid-phase microextraction and analyzed by gas chromatography-mass spectrometry (GC-MS). A total of 43 volatile components, including 15 esters, 12 terpenes, 7 alcohols, 4 acids, 3 ketones, and 2 aldehydes, were identified. Ethyl esters and alcohols such as ethyl acetate, ethyl octanoate, isoamyl alcohol, and phenethyl alcohol were the most represented groups among the quantified volatiles. In particular, various terpenes such as DL-limonene, linalool, alpha-terpineol, and myrtenol were identified. The differences in volatile components among the 24 black raspberry wines and possible sample grouping were examined by applying principal component analyses to the GC-MS data sets. The first and second principal components explained 43.9% of the total variation across the samples. No apparent sample groupings were observed according to manufacturing locations. The samples KU, BH, SR, and MO showed higher overall levels in the concentrations of terpenes originating from black raspberry, while other samples such as BB and HB, showed higher in ethyl ester and alcohol contents produced by yeast fermentation, respectively.

• KSBB Journal
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• v.24 no.5
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• pp.425-431
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• 2009

Hepatocyte growth factor (HGF) and its receptor play an important role in the formation and progression of glioma. In this study, I investigated the ability of HGF to recover of the PSA siRNA-suppressed cell proliferation, migration and invasion in U-251-MG cells. PSA siRNA-transfected U-251-MG cells showed the reduction of the proliferation, migration and invasion with compared to control. Treatment of HGF on the PSA siRNA-transfected U-251-MG cells recovered the ability of proliferation, migration and invasion. These data suggest that PSA and HGF may use unique and parallel signaling cascade leading to the proliferative, migrative and invasive phenotype of U-251-MG cells. I also showed that PSA cooperated with HGF to a migrative and invasive phenotype via the increased secretion of matrix metalloproteinase-2 (MMP-2) and MMP-9.

• Journal of Aquaculture
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• v.16 no.1
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• pp.24-30
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• 2003

Studies of the seed production of Urechis unicinctus were conducted under the laboratory conditions to obtain some information for the U.unicinitus culture. The experiment included developmental studies of the egg development, larval culture, sediment preference and growth of young U.unicinctus. The experiment were conducted from March to August, 2000. The adults of U.unicinctus collected in Namhae-do, Korea. The developments of the fertilized eggs were observed under a light-microscope at intervals of one hour after containing with density of one individual per 1 $m\ell$. The larvae were fed with Phaeodactylum tricornutum cultured at the laboratory. The concentration of the phytoplankton for the feed was 30,000 cells per individual larva. With progress of development, the food concentration was gradually increased, up to 10,000 cells per individual for the young U.unicinctus. Trochophore larvae appeared on the 68 hours after hatching. On the 32 days after hatching, over 50% of fertilized eggs developed into young U.unicinctus. In order to investigate the effect of sediment on the growth and burrowing of U.unicinctus, the young worms were reared in tanks with different grain sizes. The highest value of sediment preference and survival rate of U.unicinctus was shown in the mixture sediment group with below 0.10 mm, 1.01∼12.00 mm, over 3.01 mm and shell. The lowest value in both sediment preference and survival rate of U.unicinctus was observed in 1.0l∼2.00 mm grain size.

• Journal of Life Science
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• v.30 no.1
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• pp.58-63
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• 2020

This study was conducted to examine association between melanocortin-4-receptor (MC4R) genotypes and fatty acid (FA) composition in an F₂ intercross between Duroc and Jeju (South Korea) Native pigs (JNP). Fourteen FA composition traits were measured in more than 290 F₂ progeny population produced between Duroc and JNP. All experimental pigs were successfully genotyped for the MC4R c.1426A>G (p.Asp298Asn) single nucleotide polymorphism (SNP) by using Taq I PCR-RFLP methods. We detected three MC4R genotypes, AA, AG, and GG with 0.299, 0.542, and 0.159 genotype frequencies, respectively. The MC4R AA genotype animals showed higher levels in palmitic acid (C16:0, p<0.05), stearic acid (C18:0, p<0.01), eicosenoic acid (C20:1n9, p<0.05), saturated fatty acid (SFA, p<0.01) than GG homozygotes, respectively. Whereas MC4R GG genotype showed higher values in linoleic acid (C18:2n6, p<0.001), linolenic acid (C18:3n3, p<0.001), linolenic acid (C18:3n6, p<0.001), arachidonic acid (C20:4n6, p<0.001) and unsaturated fatty acid (USFA, p<0.01) than AA pigs, respectively. The MC4R GG genotype was associated with increasing USFA and decreasing SFA in the F₂ progeny population produced between Duroc and JNP. Our finding suggests that the MC4R polymorphisms can be used as a valuable genetic marker for Duroc and JNP breeding programs to improve meat quality and to control FA compositions.

• Proceedings of the Korean Operations and Management Science Society Conference
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• 1993.04a
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• pp.483-492
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• 1993

미래의 차의 선택사항이 될 여행정보시스템을 설치하여 그 성능의 인간공학적 조사를 수행하였다. 이 시스템을 실제로 GM자동차 Oldsmobile Toronado형 100대에 설치하여 사용자가 얼마나 쉽게, 빨리 그리고 안락하게 갈 수 있고, 어떻게 하면 좀 더 이것을 인간공학적으로 향상시킬수 있는 가를 연구하였다. 100대중 23대는 대학교에서 연구를 하고 나머지는 AAA회사의 회원권을 가진 지역사용자들에게 AAA회사와 AVIS회사를 통하여 대여를 하여 컴퓨터차(TravTek시스템)의 성능과 효율성을 시험하였다. 그 내용을 살펴보면, 차에 부착된 컴퓨터의 컬러화면으로 사용자에게 도로를 보여주고, 컴퓨터가 최단거리를 제시하여 운전자에게 컴퓨터음성과 화면으로 길을 안내한다. 그 파급효과로 도로 체증현상을 막고, 기름의 낭비도 절약하고, 밤에도 안전하게 운행할 수 있게 할 뿐만아니라 처음보는 거리라고 하더라도 컴퓨터가 안내하면서 목적지까지 무사히 도착할 수 있게 하여 준다. 이러한 시스템을 설치한 차를 타고 여행할 때, 여행자가 과연 얼마나 안락하게 여행할 수 있으며, 도로의 체증현상을 줄이고, 사고를 예방하며, 차의 설계와 목적이 인간공학적으로 합당한 가를 알아보고자 하는 연구이다. 인간공학적 평가 인자들은 (1) 운전자의 수행도, (2) 사용자 선호도, (3) 사용자 인식, (4) 운행정보등이다. 그리고 컴퓨터음성을 사용하였을 때와 사용하지 않았을 때의 두가지 경우와 (1) 움직이는 컴퓨터지도를 사용하였을 때 (2) 단순화한 도로안내를 사용하였을 때, (3) 컴퓨터 지도를 사용하지 않았을 경우(종이지도사용)에 관해 위의 4가지 인간공학적 인자들을 평가하고자 한다. 이 연구는 아직도 진행중이라 발표하고자 하는 논문역시 현재까지의 연구결과를 토대로 발표하는 것이므로 완전한 결론을 내릴 수는 없고, 진행과 정의내용과 토의사항과 잠정적인 결론을 제시하고자 한다. 의거한 작업순서 결정을 위해 우선 BB의 상한을 구하는 연구를 행했다. 이를 위해 우선 단일작업장에서 야기될 수 있는 모든 상황을 고려한 최적 작업순서 결정규칙을 연구했으며, 이의 증명을 위해 이 규칙에 의거했을 때의 보완작업량이 최소가 된다는 것을 밝혔다. 보완작업 계산의 효율성을 제고하기 위해 과부하(violation)개념을 도입하였으며, 작업유형이 증가된 상황에서도 과부하 개념이 보완작업량을 충분히 반영할 수 있음을 밝혔다. 본 연구에서 제시한 최적 작업순서 규칙에 의거했을 때 야기될 수 있는 여러가지 경우의 과부하를 모두 계산했다. 앞에서 개발된 단일작업량의 최적 작업순서 결정규칙을 이용하여 다작업장의 문제를 실험했다. 이 문제는 규모가 매우 크므로 Branch & Bound를 이용하였으며, 각 가지에서 과부하량이 최적인 경우만을 고려하는 휴리스틱을 택하여 실험자료를 이용하여 여러 회 반복실험을 행했다. 그리고 본 연구의 성과를 측정하기 위해 휴리스틱 기법시 소요되는 평균 CPU time 범위에서, 랜덤 작업순서에 따른 작업할당을 반복실험하여 이중 가장 좋은 해와 비교했다. 그러나 앞으로 다작업장 문제를 다룰 때, 각 작업장 작업순서들의 상관관계를 고려하여 보다 개선된 해를 구하기 위한 연구가 요구된다. 또한, 준비작업비용을 발생시키는 작업장의 작업순서결정에 대해서도 연구를 행하여, 보완작업비용과 준비비용을 고려한 GMMAL 작업순서문제를 해결하기 위한 연구가 수행되어야 할 것이다.로 이루어 져야 할 것이다.태를 보다 효율적으로 증진시킬 수 있는 대안이 마련되어져야 한다고 사료된다.$\ulcorner$순응$\lrcorner$의 범위를 벗어나지 않는다. 그렇기 때문에도 $\ulcorner$순응$\lrcorner$과 $\ulcorner$표현$\lrcorner$

• Clinical and Experimental Pediatrics
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• v.51 no.8
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• pp.879-885
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• 2008

Purpose : The human lung fibroblast may act as an immunomodulatory cell by providing pro-inflammatory cytokines and chemokines, which are important in airway remodeling. Vascular endothelial growth factor (VEGF) induces mucosal edema and angiogenesis. Thymus and activation regulated chemokine (TARC) induces selective migration of T helper 2 cells. We investigated whether human lung fibroblasts produced VEGF and TARC, and the effects were augmented with the co-culture of fibroblasts and human bronchial smooth muscle cells (HBSMC), and whether dexamethasone can inhibit the proliferation and the release of VEGF in lung fibroblasts. Methods : Human lung fibroblasts were cultured with and without HBSMC, growth-arrested in serum-deprived medium, and pretreated with dexamethasone for 16 hours. After 24-hour stimulation with platelet derived growth factor-BB (PDGF-BB) and/or transforming growth factor-${\beta}$ (TGF-${\beta}$), culture supernatant was harvested for assays of VEGF and TARC. Cell proliferation was assayed using BrdU cell proliferation ELISA kit. Results : 1) The release of VEGF was significantly increased after stimulation with TGF-${\beta}$, and its release was augmented when co-stimulated with PDGF and TGF-${\beta}$. 2) VEGF release induced by PDGF or TGF-${\beta}$ was inhibited by dexamethasone. 3) There was no synergistic effect on the release of VEGF when human lung fibroblasts were co-cultured with HBSMC. 4) Dexamethasone did not suppress human lung fibroblasts proliferations. 5) Neither TGF-${\beta}$ nor PDGF induced TARC release from lung fibroblasts. Conclusion : Human lung fibroblasts may modulate airway remodeling by release of VEGF, but they have no synergistic effects when co-cultured with HBSMC. Dexamethasone suppresses VEGF release, not proliferation of lung fibroblast.

• Korean Journal of Breeding Science
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• v.40 no.4
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• pp.443-446
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• 2008

'Gangbaek' is a japonica rice variety developed and registered by the rice breeding team of Department of Rice and Winter Cereal Crop, NICS, RDA in 2006. 'Gangbaek' was derived from a cross between 'Suweon345' with good grain quality and 'DV85' resistant to bacterial blight, $K_{3a}$. $F_1$ plants were grown in the greenhouse in winter of 1992/1993 and backcrossed with 'Suweon345' as the recurrent parent. Plants resistant to $K_{3a}$ race of bacterial blight (BB) were selected from $BC_1F_1$ to $BC_4F_1$ and used as parents in the backcrossing processes. This variety has about 120 days growth duration from transplanting to harvesting in west-southern coast and Honam plain of Korea. It is about 69 cm in culm length and tolerance to lodging. In reaction to biotic and abiotic stresses, it shows moderately resistance to blast, and resistance to bacterial blight pathogen, $K_1$, $K_2$, $K_3$ and $K_{3a}$, but susceptible to other major diseases and insect pests. The milled rice of 'Gangbaek' exhibits translucent, relatively clear non-glutinous endosperm and midium short grain. It has lower amylose content of 18.6% and protein content of 6.4% compared with 'Nampyeongbyeo'. The milled rice yield performance of this variety is about 5.28 MT/ha in local adaptability test for three years. This cultivar would be adaptable to the bacterial blight-prone area in the south-western coastal and Honam plain of Korea.

• Proceedings of the KSR Conference
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• 2011.10a
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• pp.1251-1262
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• 2011

K-company, being in charge of domestic railway construction and facilities management, got a success rate of 41% with the implement of a improvement scheme by prosecuting of 6 Sigma and the achievement of CTQ (Success criteria : more than 0.5 in achievement of CTQ). It is clear that the factors having an effect on achievement of CTQ are the level of project when pushing forward the project(Big Y and small y according to the scope of the work), the degree of interest of an officer in charge like sponsors, and the continuous feedback toward the implement of a improvement scheme. For improvement CTQ achievement, firstly redefine about a type of project. Secondly, derive small y by Big Y and derives a unit work by small y. Then grouping the unit works and achieve Big Y by performing of every unit work as an executive subject. Thirdly organize a committee of subject selection which is supervised by the general manager. Therefore exhibit staff's leadership, for example motivation, by strong incentives. Lastly, provide ongoing learning and enhance system monitoring about a result management of an betterment execution department.