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수확 후 저장 배에서 분리한 Penicillium속 균의 유전적 다양성 (Genetic Diversity of Penicillium isolates Isolated from Pears with Postharvest Decay in Storage)

  • 한도숙;홍성기;강희완
    • 한국균학회지
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    • 제40권1호
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    • pp.11-18
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    • 2012
  • 평택일원의 6개의 배 저장고로부터 300개의 부패된 배를 수집 하여 병반부위로부터 곰팡이를이분리 하여 조사한 결과 Penicillium 균이 이병배중에서 84%가 분리되었다. 84 Penicillium 균주를 대상으로 URP2R primer로 균주 간의 DNA 다양성을 조사한결과 18 type의 Penicillium 균주를 검출 할 수 있었으며 URP1F, URP2F, URP2R, URP4R의 PCR 다형성밴드를 이용한 유전적 유연관계분석으로 4 group으로 나눌 수 있었다. 18 Penicillium균주의 형태적특징을 조사한결과 3가지의 특징적인 배양적 특징을 갖는 것으로 확인 되었으며 포자형성과 포자형태에서도 3가지 type이 관찰 되었는데 SP-15균주 type, KP-1-1 균주 type, SP-17 type으로 PCR 다형성결과와 유사한 경향을 나타내었다. SP-15형은 형태적, rDNA의 ITS 염기서열 분석에서 P. expansum로 동정 되었으며 분리된 Penicillium 균주 중 70%를 차지하는 우점종인 것으로 나타났으므로 향 후 URP-PCR profile은 종간, 종내 계통 분류에 유용하게 이용 할 수 있다.

보육교사의 자아존중감, 직무스트레스가 교사효능감에 미치는 영향 (Influencing Factors of Self-esteem and job stress on Teacher Efficacy for Childcare Teacher)

  • 변은경;장경오
    • 한국산학기술학회논문지
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    • 제16권6호
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    • pp.3982-3990
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    • 2015
  • 본 연구는 보육교사의 교사효능감의 정도를 알아보고 자아존중감과 직무스트레스가 교사효능감에 미치는 영향을 파악한 연구이다. 연구대상자는 G도에서 보육교사로 근무하는 280명을 대상으로 하였고, 자료분석은 SPSS WIN/21.0을 이용하여 평균과 표준편차, t-검정, 일원분산분석, 피어슨 상관계수, 다중회분귀분석을 사용하였다. 연구결과는 보육교사의 교사효능감은 $3.59{\pm}0.38$점으로 나타났다. 교사효능감은 연령(F=3.493, p=.016), 경력(F=5.654, p=.001), 직위(F=6.681, p<.001), 학급연령(F=2.759, p=.043), 자녀양육경험(t=5.269, p=.022)에서 유의한 차이를 나타냈다. 연구대상자의 교사효능감은 자아존중감과 순상관관계(r=.236, p<.001), 교사효능감과 직무스트레스는 역상관관계(-.251, p<.001)가 있는 것으로 나타났다. 또한 교사효능감에 영향을 미치는 요인은 자아존중감과 직무스트레스로 확인되었고 이러한 요인들은 보육교사의 교사효능감을 11.7%로 설명하였다. 보육교사의 교사효능감을 높이기 위해 반복연구를 통하여 교사효능감에 영향을 미치는 요인을 확인하고, 교사효능감을 높일 수 있는 실제적인 중재들이 요구된다.

인공탈회된 법랑질에 대한 카제인나트륨의 재광화 효과 (Remineralization effect of sodium caseinate on artificially demineralized enamel)

  • 임희정;정임희;임도선
    • 대한치위생과학회지
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    • 제5권1호
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    • pp.29-38
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    • 2022
  • Background: This study aimed to compare the remineralization effects of sodium caseinate and other substances on artificially demineralized enamel. Methods: We selected 25 healthy human premolars and molars and produced a total of 75 specimens by dividing them into five groups: control group, with distilled water; experimental group 1 (EG1), with 3% sodium caseinate; EG2, with 10% sodium caseinate; EG3, with casein phosphopeptide-amorphous calcium phosphate (CPP-ACP); and EG4, with 0.05% NaF. Subsequently, the specimens were immersed in an artificial demineralization solution for 60 min. The demineralized specimens were then immersed in a remineralization solution for 7 days. Surface microhardness was measured using a microhardness tester, and remineralized lesions were observed under a scanning electron microscope (SEM). Regarding statistical analysis, the paired t-test and analysis of variance were performed using the SPSS program. Results: Although the surface hardness of the remineralized lesions increased significantly in all groups (p<0.05), the average increment did not differ significantly between the groups. The surface microhardness of CPP-ACP was the highest, followed by that of 0.05% NaF and 10% sodium caseinate. The remineralization effect of sodium caseinate was similar to that of 0.05% NaF. SEM confirmed that all groups treated with the remineralization solution were remineralized. Conclusions: Although the remineralization effect of sodium caseinate was slightly lower than that of CPP-ACP, it was similar to that of 0.05% NaF. Therefore, to enhance the remineralization effect of sodium caseinate, the appropriate concentration and application time should be determined.

한국산 적포도주의 관능적 특성에 관한 연구 (III) (A Study on the Sensory Characteristics of Korean Red Wine)

  • 이장은;홍희도;최희돈;신용섭;원유동;김성수;고경희
    • 한국식품과학회지
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    • 제35권5호
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    • pp.841-848
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    • 2003
  • 국내산 포도품종을 이용하여 G(거봉100%), M(머루100%), C(캠벨100%), GM(거봉70%+캠벨30%), GC(거봉70%+캠벨30%) 포도주를 제조하여 프랑스산 적포도주 (Cabernet Sauvignon, 1998)와 함께 색도, 향기성분, 맛, 종합평가에 관한 선호도를 순위법(ranking test)으로 측정하고, 분석적 관능평가 방법으로 평가하였다. C(캠벨)는 4.76으로 색도면에서 가장 높은 선호도를 보였으며, 다음으로는 GM(거봉+머루)이 3.94, F(프랑스)가 4.67, M(머루)이 3.70, GC(거봉+캠벨)가 2.65, G(거봉)가 1.47로 높은 선호도 순서를 보였다(p<0.001). 향기성분의 평가에서는 M이 3.94, C가 3.76, GM이 4.12, F가 3.76으로 이들은 유의적으로 가장 높은 선호도를 보였으며, 3.12를 얻은 GC와, 2.29를 얻은 C는 이들보다 낮은 선호도를 보였다(p<0.01). 맛의 평가에서는 4.75를 얻은 F와, 4.25를 얻은 C에 이어, GM이 3.37, GC가 3.50, G가 2.75, M이 2.37로 높은 선호도 순서를 보였다(p<0.001). 색도, 향기성분, 맛을 바탕으로 한 전체적인 종합 평가에서는 F가 4.81, C가 4.06, GM이 3.87로 각각 높은 선호도를 보였으며 다음으로는 3.64를 얻은 GC, 2.37을 얻은 G, 2.44를 얻은 M의 순이었다(p<0.001). 전체적인 종합평가에 가장 높은 영향을 준 인자는 맛 항목으로 69.2%를 설명할 수 있었다. 다음은 색도와 향기성분 순이었으며, 이들 세 항목으로 포도주의전체적 종합평가를 74.4% 설명할 수 있었다. 회귀분석 결과 purple과 red color는 색의 선호도를 각각 28.9%, 8.5% 설명할 수 있었으며, grape flavor는 향기성분에 관한 선호도를 14.4% 설명할 수 있었고 sweet, acid, bitter, salty taste들은 맛의 선호도를 17.3% 설명할 수 있었다. 상관관계 결과 purple과 red 항목은 각각 grape taste, floral taste와 그리고 grape, oak, flavor들과도 양의 상관관계를 보였고, grape flavor는 floral flavor, grape taste, floral taste들과 양의 상관관계를 나타냈으며(p<0.001), $SO_2$향과는 음의 상관관계를 보였다(p<0.001). Sweet taste는 grape, floral flavor와 양의 상관 관계를 보였으며(p<0.001), bitter, astringency taste와는 음의 상관관계를 나타내어(p<0.01), sweet taste가 강할수록 바람직한 향기성분과 맛의 강도가 강하게 평가되었으며, 또한 이들 항목의 점수가 높을수록 선호도도 높이 평가되어 sweet taste, grape, floral flavor와 bitter, astringency taste의 조화는 적포도주의 관능적인 면에서 중요한 요소들임을 알 수 있었다.

Cloning and Sequencing of the ${\alpha}-1{\rightarrow}6$ Dextransurcrase Gene from Leuconostoc mensenteroides B-742CB

  • Kim, Ho-Sang;Kim, Do-Man;Ryu, Hwa-Ja;Robyt, John-F.
    • Journal of Microbiology and Biotechnology
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    • 제10권4호
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    • pp.559-563
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    • 2000
  • A dextransucrase gene (dsrB742) that expresses a dextransucrase to synthesize mostly ${\alpha}-1{\rightarrow}6$ linked dextran with a low amount (3-5%) of ${\alpha}-1{\rightarrow}3$ branching was cloned and sequenced from Leuconostoc mesenteroides B-742CB. The 6.1-kb PstI fragments were ligated with pGEM-3Zf(-) and transformed into E. coli $DH5{\alpha}$. The recombinant clone (pDSRB742) synthesized dextran on an agar plate containing 2% (w/v) sucrose. The dextran synthesized was hydrolyzed with Penicillium endo-dextranase. The hydrolyzate was composed of glucose, isomaltose, isomaltotriose, and branced pentasaccharide. The nucleotide sequence of dsrB742 showed one open reading frame (ORF) composed of 4,524 bp encoding dextrasnsucrase. The deduced amino acid sequence revealed a calculated molecular mass of 168.6 kDa. It also showed an activity band of 184 kKa on a non-denaturing SDS-PAGE (10%). The amino acid sequence of DSRB742 exhibited a 50% similarity with DSRA from L. mesenteroides B-1299, a 70% similarity with DSRS from L. mesenteroides B-512 (F, FMCM) and a 45-56% similarity with Streptococcal GTFs.

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京安川 流域의 土地利用에 따른 河川物質의 變化 (Changes in Stream Water Quality According to Land Use at Kyong-an Stream)

  • Yim, Yang-Jai;Bang, Je-Yong;Kim, Yoon-Dong
    • The Korean Journal of Ecology
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    • 제18권3호
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    • pp.341-351
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    • 1995
  • The relationship between land uses and water quality was investigated at Kyong-an Stream. Some 70% of this watershed was forested area, half of which was comprised of Pinus densilflora community. Concentrations of $NH_4^{+},\;NO_3^{-},\;NO_2^{-}, total nitrogen, $Cl^{-},\;PO_4^{3-}$, DO, and BOD increased gradually from upstream to downstream, whereas heavy metals did not have such tendancy with the exception of a few sites. Urban area was significantly correlated with hardness and chloride concentration. Relationship among phosphate concentration(P), cultivated field area(F), and stream length(S) in each basin was P = 1.7912 F/S+0.0103. the concentration of $NH_4^{+}$ was positively correlated with the population size and cow density within the catchment. The effect of urban area(U) and stream length of the pH(pH) was represented by pH = -4.7344 U/S+6.52. It can be concluded that the control of nonpoint source pollution as well as point source pollution is one of the important problems of water quality management, especially geological properties must be considered for sustainable development.

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The Possibility of 1,3,4-Oxadiazole Containing Polymer as a New Polymer Electrode in Redox Supercapacitor

  • Ryu, Kwang-Sun;Chang, Soon-Ho;Kwon, Soon-Ki;Kim, Yun-Hi;Hwang, Do-Hoon
    • Macromolecular Research
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    • 제10권1호
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    • pp.40-43
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    • 2002
  • Poly(1', 4'-phenylene-1", 4"-(2"-(2""-ethyl-hexyloxy)) phenylene-1",4"-phenylene-2,5-oxadiazolyl) (PPEPPO) was synthesized and its electrochemical characteristics was investigated as electrode material in redox supercapacitor. The cyclic voltammetry (CV) shows there was scarcely a redox reaction and further suggests n-doping is difficult to occur in this system. However, the discharge curve between 3.0 to 0.01 V is continuously decreased like a straight line, similar to the discharge pattern of EDLC. The initial specific discharge capacitance is ~6.4 F/g, while the specific capacitance of 1000th cycle is ~0.1 F/g. The PPEPPO can be used as the electrode of supercapacitor, emissive material, as well as charge-transporting material in polymer LED.ansporting material in polymer LED.

The Characteristics of pulverized Powder by Automatic Tablet Crusher

  • Doh, Seong-Tak;Kim, Sung-Hwan;Lee, Do-Young;Kwon, Oh-Ju
    • 대한의생명과학회지
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    • 제8권2호
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    • pp.101-106
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    • 2002
  • The purpose of this study was to test the capacity of automatic tablet crusher. The Automatic tablet crusher not only saved crushing time, but also removed floating fine powders that was occurred during crushing and caused allergy. The characteristics of powder produced by this crusher were as follows: First, to meet the condition as powder after pulverizing 5∼10 g tablet it took 6 to 12 seconds crushing time. But six seconds would do to get powder f3r oral administration. Second, when the quantity of medicine was measured by HPLC, powder from automatic crusher showed higher than that from pestle. HPLC chromatogram indicated that there was no change of medicine in the process of pulverization.

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단양 고수동굴과 여천굴의 환경요인과 그 동물상에 관한 연구 (Studies on Environmental Factors and Faune of Kosudong-gul Cave and Yoe chon-gul Cave in Dan-yang gun, Korea.)

  • 임문순
    • 동굴
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    • 제1권1호
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    • pp.17-22
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    • 1975
  • The Kosudong-gul Cave at kosu-ri, Daegang-myon, Danyang-gun, Chung Chong Bug-do, Province was surveryed by this author on the 3rd of Nov. 1973, 6th of Jan. 1974, 30th of Aug. and 13th of Sept. 1975 through over several times field trips. Yoechon-gul Cave at Yoechon-ri, Gagok-myon, Danyang-gun, Chung Chong Bug-do, Province was surveyed by this author on the 30th of August, 1975. The enviromental factors of both caves checked the siftations for air temperature, water temperature, moisture and pH, as well as the faunae f both caves and ecollogically studieel them. Kosudong-gul Cave (1) The environmental factors checked was the same as table 1. (2) The fauna is 5 classes and 18 species and they classified as Troglobite......2 species-11.1%, troglophile.....5 species-27.7%, and trogloxene....11 species.-61.2%. Yochon-gul Cave (1)The environmental factor Checked was the same as table 2. (2) The fauna is 5 classes and 8 species and they classified as troglobite......1 species-11.1%, troglobite....4 species-44.4% and trogloxene.....4 species-44.4%.

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2-레그 3상 PWM 인버터의 출력전압에서 직류링크 리플전압의 영향 보상 (Compensation of Effects of DC-Link Ripple Voltages on Output Voltage of Two-Leg Three-Phase PWM Inverters)

  • 김영신;이동춘;석줄기
    • 대한전기학회논문지:전기기기및에너지변환시스템부문B
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    • 제55권1호
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    • pp.47-53
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    • 2006
  • In this paper, a simple scheme compensating for the effect of dc-link ripple voltages on output voltage of two-leg and three-phase PWM inverters is proposed, where single-phase half-bridge PWM convertor and two-leg inverter are used. The voltage at neutral point of the dc-link is controlled so that the upper-half of do-link voltage is equal to the lower-half voltage in average concept. However, the effect of the do-link ripple voltage results in the inverter output voltage and current distortion. This effect can be eliminated by introducing a compensation voltage in switching time calculation. Also, the inverter dead time should be compensated for sinusoidal output waveform. The proposed scheme has been verified by experimental results which were obtained from the V/F constant operation of the induction motor fed by two-leg inverter.