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http://dx.doi.org/10.4489/KJM.2012.40.1.011

Genetic Diversity of Penicillium isolates Isolated from Pears with Postharvest Decay in Storage  

Han, Do-Suk (Graduate School of Bio.&Information Technology, Hankyong National University)
Hong, Sung-Kee (National Academy of Agricultural Science, RDA)
Kang, Hee-Wan (Graduate School of Bio.&Information Technology, Hankyong National University)
Publication Information
The Korean Journal of Mycology / v.40, no.1, 2012 , pp. 11-18 More about this Journal
Abstract
This study was carried out to identify the genetic diversity of Penicillium isolates that were isolated from pears with postharvest decay in storage. URP-PCR was used to detect DNA diversity of 84 Penicillium isolates. Based on URP-PCR profiles, 18 Penicillium isolates were selected and their PCR polymorphic bands were produced by additional primers URP1F, URP2R, URP2F, and URP4R. UPGMA cluster analysis using the polymorphic bands showed four clustered groups and futhermore cultural and morphological features characterized the 18 Penicillium isolates. Group 1 was dominant, which occupies 70% in the four clustered groups and identified as P. expansum based on ITS sequence and morphological features.
Keywords
PCR polymorphism; Pear; Penicillium isolates; Postharvest decay;
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Times Cited By KSCI : 7  (Citation Analysis)
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