• Asian Pacific Journal of Cancer Prevention
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• 제17권4호
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• pp.1967-1971
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• 2016

Background: In tumor cells, aberrant differentiation programs have been described. Several neuronal proteins have been found associated with morphological neuronal-glial changes in breast cancer (BCa). These neuronal proteins have been related to mechanisms that are involved in carcinogenesis; however, this regulation is not well understood. Microtubule-associated protein-tau (MAP-Tau) has been describing in BCa but not its variants. This finding could partly explain the neuronal-glial morphology of BCa cells. Our aim was to determine mRNA expression of MAP-tau variants 2, 4 and 6 in breast cancer cell lines. Materials and Methods: Cultured cell lines MCF-10A, MDA-MB-231, SKBR3 and T47D were observed under phase-contrast microscopy for neural morphology and analyzed for gene expression of MAP-Tau transcript variants 2, 4 and 6 by real-time PCR. Results: Regarding morphology like neural/glial cells, T47D line shown more cells with these features than MDA-MB-231 and SKBR. In another hand, we found much greater mRNA expression of MAP-Tau transcript variants 2, and to a lesser extent 4 and 6, in T47D cells than the other lines. In conclusion, regulation of MAP-Tau could bring about changes in cytoskeleton, cell morphology and motility; these findings cast further light on neuronal transdifferentiation in BCa.

• The Korean Journal of Physiology and Pharmacology
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• 제17권4호
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• pp.259-265
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• 2013

The anti-apoptotic effect of (-)-epigallocatechin-3-gallate (EGCG) during unilateral testicular torsion and detorsion (TT/D) was established in our previous study. In mice, the smallest inhibitor of apoptosis, survivin, is alternatively spliced into three variants, each suggested to have a unique function. Here, we assessed how EGCG exerts its protective effect through the expression of the different survivin splice variants and determined its effect on the morphology of the seminiferous tubules during TT/D. Three mouse groups were used: sham, TT/D+vehicle and TT/D treated with EGCG. The expression of the survivin variants (140 and 40) and other apoptosis genes (p53, Bax and Bcl-2) was measured with semi-quantitative RT-PCR. Histological analysis was performed to assess DNA fragmentation, damage to spermatogenesis and morphometric changes in the seminiferous tubules. In the TT/D+vehicle group, survivin 140 expression was markedly decreased, whereas survivin 40 expression was not significantly different. In parallel, there was an increase in the mRNA level of p53 and the Bax to Bcl-2 ratio in support of apoptosis induction. Histological analyses revealed increased DNA fragmentation and increased damage to spermatogenesis associated with decreased seminiferous tubular diameter and decreased germinal epithelial cell thickness in the TT/D+vehicle group. These changes were reversed to almost sham levels upon EGCG treatment. Our data indicate that EGCG protects the testis from TT/D-induced damage by protecting the morphology of the seminiferous tubules and modulating survivin 140 expression.

• 한국수산과학회지
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• 제51권4호
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• pp.404-410
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• 2018

Takifugu rubripes, T. chinensis, T. pseudommus and an unidentified species were morphologically and genetically studied. Morphology was compared using external morphology and the genetic analysis was conducted using mitochondrial DNA cytochrome oxidase I (COI) and cytochrome b (Cyt b). The morphological characteristics of T. rubripes, T. chinensis and T. pseudommus were confirmed, as reported previously. The unidentified species was confirmed to have the characteristics of T. rubripes and T. chinensis. The COI sequences of the four species were 99-100% similar; genetic distance was d=0.0000 and Cyt b genetic distance was d=0.0000-0.00834. Species in the phylogenetic tree belonging to the same group could not be classified genetically. In conclusion, the unidentified species was considered to be a hybrid between T. rubripes and T. chinensis.

• 대한소아치과학회지
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• 제30권1호
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• pp.132-142
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• 2003

본 연구는 Hellman 치령 IIIA기에 해당되는 전치부 반대교합아동의 구개형태에 대한 특징을 3차원 레이저 스캐너를 이용하여 조사한 것이다. 반대교합아동과 정상교합아동 각각 20개의 상악 석고모형을 채득하였다. 각 모형은 3차원 레이저 스캐너(Intertec, Korea)로 스캔한 후 rapidform 2000 프로그램 (INUS, Korea)으로 3차원 이미지를 얻었으며, Rhino 3D 프로그램(rhinoceros, USA)으로 수치화 하였다. 측정부위는 양측 유견치 및 제1대구치를 연결한 횡단면상의 구개형태 곡선 그리고 절치유두의 최정점에서 양측 제1대구치 원심면까지의 구개봉합선을 따르는 구개형태곡선들이었다. 개체들간의 크기 차이 보정을 위해 측정부위를 각각 25mm, 35mm, 35mm로 조정하여 표준화 하였다. 표준화된 구개형태곡선들에서 직선거리 각 1mm에 해당되는 점들에서의 곡선 깊이를 소수점 3자리까지 수치화 하여 평균 구개형태곡선을 얻고, 그 좌표 차이를 95% 신뢰구간의 t-test를 통해 비교, 분석하여 다음과 같은 결론을 얻었다. 1. 양측 유견치를 연결한 횡단면상의 평균구개형태곡선은 반대교합아동이 좀더 평탄한 V자형의 곡선형태를 보였으나, 반대교합아동과 정상교합아동 사이에 통계적으로 유의할 만한 차이는 보이지 않았다(P>0.05). 2. 양측 제1대구치를 연결한 횡단면상의 평균구개형태곡선은 반대교합아동이 정상교합아동보다 더 구개의 깊이가 깊었으며, 상악 우측 제1대구치 치은 최하방점에서부터 8mm와 21mm위치 사이의 구간에서 통계적으로 유의할 만한 차이가 있었다(P<0.05). 3. 반대교합아동과 정상교합아동간의 구개봉합선의 평균구개형태곡선에서는 전구간에 걸쳐 반대교합아동이 더 깊은 곡선 형태를 보였고, 통계적으로도 유의할 만한 차이를 보였다(P<0.01).

• Ocean Systems Engineering
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• 제6권1호
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• pp.61-97
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• 2016

This is the second of two papers on the 3D numerical modeling of nearshore hydro- and morphodynamics. In Part I, the focus was on surf and swash zone hydrodynamics in the cross-shore and longshore directions. Here, we consider nearshore processes with an emphasis on the effects of oceanic forcing and beach characteristics on sediment transport in the cross- and longshore directions, as well as on foreshore bathymetry changes. The Delft3D and XBeach models were used with four turbulence closures (viz., ${\kappa}-{\varepsilon}$, ${\kappa}-L$, ATM and H-LES) to solve the 3D Navier-Stokes equations for incompressible flow as well as the beach morphology. The sediment transport module simulates both bed load and suspended load transport of non-cohesive sediments. Twenty sets of numerical experiments combining nine control parameters under a range of bed characteristics and incident wave and tidal conditions were simulated. For each case, the general morphological response in shore-normal and shore-parallel directions was presented. Numerical results showed that the ${\kappa}-{\varepsilon}$ and H-LES closure models yield similar results that are in better agreement with existing morphodynamic observations than the results of the other turbulence models. The simulations showed that wave forcing drives a sediment circulation pattern that results in bar and berm formation. However, together with wave forcing, tides modulate the predicted nearshore sediment dynamics. The combination of tides and wave action has a notable effect on longshore suspended sediment transport fluxes, relative to wave action alone. The model's ability to predict sediment transport under propagation of obliquely incident wave conditions underscores its potential for understanding the evolution of beach morphology at field scale. For example, the results of the model confirmed that the wave characteristics have a considerable effect on the cumulative erosion/deposition, cross-shore distribution of longshore sediment transport and transport rate across and along the beach face. In addition, for the same type of oceanic forcing, the beach morphology exhibits different erosive characteristics depending on grain size (e.g., foreshore profile evolution is erosive or accretive on fine or coarse sand beaches, respectively). Decreasing wave height increases the proportion of onshore to offshore fluxes, almost reaching a neutral net balance. The sediment movement increases with wave height, which is the dominant factor controlling the beach face shape.

• 한국결정성장학회:학술대회논문집
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• 한국결정성장학회 1997년도 Proceedings of the 13th KACG Technical Meeting `97 Industrial Crystallization Symposium(ICS)-Doosan Resort, Chunchon, October 30-31, 1997
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• pp.123-127
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• 1997

Various recrystallization techniques has been applied to control morphology and size of NTO(3-nitro-1, 2, 4-triazole-5-one) crystals. With cooling method, it was found that the size of NTO at aqueous solution was controlled in the range of 5 to 500 $\mu\textrm{m}$. The spherical or cubic shapes of particles were obtained by adjusting operating conditions. Hexagonal and cubic shaped crystals of NTO were also obtained by sonication and evaporative method using aqueous solution of NTO. Their particle sizes were ranged 20 to 30 $\mu\textrm{m}$. In gas anti-solvent method with NTO/DMF and NTO/DMSO solutions, cubic type of NTO was obtained and the range of their sizes was 0.5-2$\mu\textrm{m}$.

• 열처리공학회지
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• 제33권1호
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• pp.1-12
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• 2020

In this study, thin foil fabrication using Ni/Ni₃Al single crystal was performed by cold-rolling. It was found that the cold-rolling behavior was strongly dependent on the initial crystallographic orientation rather than morphology of Ni₃Al precipitates. The deformation banding was formed in the case of (100)[001]- and (210)[001]-oriented specimens at 83% reduction in thickness. However, the effects of Ni₃Al precipitates morphology on the microstructure evolution of Ni/Ni₃Al single crystals during cold-rolling were not so serious comparing with the effects of initial crystallographic orientation. Therefore, it could be concluded that the deformation behavior of Ni/Ni₃Al single crystals at serious strain level was strongly dependent on the initial crystallographic orientation rather than the morphology of Ni₃Al precipitates, whereas the initial deformation behavior was related to both crystallographic orientation and the morphology of Ni₃Al precipitates.

• 한국표면공학회지
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• 제37권1호
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• pp.13-21
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• 2004

The deposition rate, the current efficiency and surface morphology of trivalent Cr deposits were investigated according to additives in sulfate bath and current density. The deposition rate of the Cr deposits plated from the formic acid complexed bath was noticeably higher than that of the glycine complexed bath. The current efficiency of the deposition from formic acid bath was higher than that of the glycine bath and increased with current density in the range of 20-50 A/d$\m^2$. The current efficiency of the deposition from the formic acid bath with boric acid buffer was higher than that of the bath with aluminum sulfate or boric acid-aluminum sulfate buffers. The nodular crystallite size of the Cr deposits increased with current density and the uniform and crack-free growth of the deposits for the aluminum sulfate was observed compared to the other buffers.

• 대한치과교정학회지
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• 제26권5호
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• pp.601-611
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• 1996

교정치료에 있어서 악안면 성장의 양상은 진단과 치료계획 수립에 있어서 매우 유용한 자료이며 교합의 달성 및 안모의 형태, 발육에 많은 영향을 미친다. 따라서 성장유형의 분류에 대한 많은 연구가 있었으며 성장예측을 위한 많은 시도가 있었다. 이에 본 연구에서는 성인 120명을 대상으로 치료전 측모두부계측 방사선사진을 이용하여 전안면고경에 대한 후안면고경의 비를 측정하여 $56\%-62\%$는 시계방향 성장군(36명), $65\%-80\%$는 반시계방향 성장군(43명)으로, 그리고 $62\%-65\%$는 정상군(41명)으로 분류하고 이에 따른 하악이부의 형태와 돌출정도 평가를 시행한 결과 다음과 같은 결과를 얻었다. 1. 남녀 하악이부 형태비교에 있어서는 남자가 여자보다 이부의 높이와 돌출정도가 크게 나타났다. 2. 시계방향 성장군이 반시계방향 성장군에 비해 하악이부의 Height, H/D ratio, Actual length는 크게 나타났고, Depth, Angle, effective length, E/A ratio는 작은 값을 나타냈다. 3. 악안면 성장양상에 있어 이부의 돌출정도가 작을수록 시계방향성장 경향을 나타내며, 돌출정도가 클수록 반시계방향성장 경향을 나타냈다.

• Asian-Australasian Journal of Animal Sciences
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• 제20권3호
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• pp.399-404
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• 2007

To elucidate the efficacy of different levels of microbial-fermented soy proteins (FSP) on piglet performance, a total of 240 weaned piglets ($L{\times}Y{\times}D$, $22{\pm}3$ d of age, $5.16{\pm}0.07$ kg initial BW) were allotted to 4 treatment diets comprising control, FSP-3%, FSP-6% and FSP-9%. The fermented soybean product named $Pepsoygen^{(R)}$ was utilized for the study. There were 15 pigs per pen and 4 pens per treatment. The control diet contained 15% soybean meal (SBM), and SBM for the treatment diets was replaced at 3, 6 and 9% with FSP, respectively. Experimental diets were fed from 0 to 14 d (phase-I) after weaning and then a common commercial diet was fed from 15 to 35 d (phase-II). There was a linear (p<0.05) increase in ADG and ADFI at both phases of measurement. The feed to gain ratio was also improved, showing a linear (p<0.01) trend as the level of supplementation increased. Except for phosphorus, the digestibility of all other nutrients was improved linearly (p<0.05) in the FSP added diets. However, villous height and crypt depth were not affected by dietary treatments. No special effect on intestinal morphology was noticed between FSP-added and control diets. In conclusion, the growth, digestibility of nutrients and morphological changes in weaned pigs fed FSP showed improved performance at higher levels of supplementation.