• Journal of Preventive Medicine and Public Health
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• 제35권4호
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• pp.282-286
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• 2002

Objectives : The level of 4-hydroxyproline (4-Hyp) in human urine was measured using high performance liquid chromatography (HPLC) with a fluorescence detector. This method is useful for medical examinations and investigating the radicals induced by physical, chemical, mental stresses. This method is superior to many published several methods in terms of its low cost and ability to analyze many samples. Methods : The urine from workers in a tire manufacturing company (22 male pre- and post-shift workers) and 18 office-workers as controls were analyzed. Data concerning age, the cumulative drinking amount and the cumulative smoking amount was collected with a questionnaire. The optimum applied amount of dansyl-Cl, the optimum reaction temperature and time, the recoveries and the optimum pH of the eluent and buffer were determined.4-Hyp from human urine was derivatized with dansyl-Cl (dimethylamino-naphthalene-1-sulfonyl chloride) after removing the a-amino acid by a treatment with phthalic dicarboxaldehyde (OPA) and cleaned with Bond Elut C18 column. The 4-Hyp derivatives were separated on a reversed phase column by gradient elution with a phosphate buffer (5 mmol, pH 8.0) and acetonitrile, and detected by fluorescence measurements at 340 nm (excitation) and 538 nm (emission). Results : The detection limit for the urinary free 4-Hyp was $0.364{\mu}mol/l$. The recovery rate of 4-Hyp was 99.7%, and the effective pH of the phosphate buffer and borate buffer were 3.0 and 8.0, respectively. From statistical analysis, age, drinking and smoking did not affect the urinary free 4-Hyp in both the controls and workers. The range of urinary 4-Hyp in the controls, pre-shift, and post-shift workers were 0.33-16.44, N.D-49.06, and $0.32-56.27{\mu}mol/l$. From the pared-sample t-test, the urinary 4-Hyp levels in post-shift workers ($11.82{\pm}6.73\;nmmol/mg\;Cre$) were 2-fold higher than in pre-shift workers ($5.36{\pm}5.53\;nmol;/mg\;Cre$) and controls ($4.91{\pm}4.89\;nmol;/mg\;Cre$). Conclusions : This method was developed with high sensitivity, accuracy, and precision. The present method was effectively applied to analyze the urinary free 4-Hyp in both controls and workers.

• Molecules and Cells
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• 제40권2호
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• pp.109-116
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• 2017

Soybean agglutinin (SBA) is an anti-nutritional factor of soybean, affecting cell proliferation and inducing cytotoxicity. Integrins are transmembrane receptors, mediating a variety of cell biological processes. This research aims to study the effects of SBA on cell proliferation and cell cycle progression of the intestinal epithelial cell line from piglets (IPEC-J2), to identify the integrin subunits especially expressed in IPEC-J2s, and to analyze the functions of these integrins on IPEC-J2 cell cycle progression and SBA-induced IPEC-J2 cell cycle alteration. The results showed that SBA lowered cell proliferation rate as the cell cycle progression from G0/G1 to S phase (P < 0.05) was inhibited. Moreover, SBA lowered mRNA expression of cell cycle-related gene CDK4, Cyclin E and Cyclin D1 (P < 0.05). We successfully identified integrins ${\alpha}2$, ${\alpha}3$, ${\alpha}6$, ${\beta}1$, and ${\beta}4$ in IPEC-J2s. These five subunits were crucial to maintain normal cell proliferation and cell cycle progression in IPEC-J2s. Restrain of either these five subunits by their inhibitors, lowered cell proliferation rate, and arrested the cells at G0/G1 phase of cell cycle (P < 0.05). Further analysis indicated that integrin ${\alpha}2$, ${\alpha}6$, and ${\beta}1$ were involved in the blocking of G0/G1 phase induced by SBA. In conclusion, these results suggested that SBA lowered the IPEC-J2 cell proliferation rate through the perturbation of cell cycle progression. Furthermore, integrins were important for IPEC-J2 cell cycle progression, and they were involved in the process of SBA-induced cell cycle progression alteration, which provide a basis for further revealing SBA anti-proliferation and anti-nutritional mechanism.

• 대한두경부종양학회지
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• 제19권1호
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• pp.58-62
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• 2003

Purpose: To evaluate the efficacy of positron emission tomography with 2-[F-18] fluoro-2-deoxy-D-glucose in discrimination of response in the nasopharyngeal carcinoma patients who treated with radiotherapy. Methods and Materials: Twenty-four patients who underwent FDG-PET scan before and after radiotherapy for no disseminated head and neck carcinoma at the Asan Medical Center between August 2001 and September 2002 were evaluate by prospective analysis. First FDG-PET scan performed before radiotherapy within 1 month, and second FDG-PET scan performed 1 month after radiotherapy. FDG-PET images were analyzed by standard uptake value (SUV). Follow-up period was more than 6 months. Results: The pretreatment SUV was 3.4-14.0 (median: 6.0) and posttreatment SUV was ground level-7.7 (median: 2.0). The overall sensitivity and specicity of FDG-PET to evaluate residual tumors in the nasopharyngeal carcinoma patients were 94% and 94%. Conclusion: FDG-PET is effective in evaluation of radiation response in the nasopharyngeal carcinoma. We think that the timing of one month after finished radiotherapy FDG-PET scan was not too fast to evaluation of radiation response.

• 한국식품영양학회지
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• 제30권5호
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• pp.1025-1034
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• 2017

As the consumption of wheat has increased recently, the number of people who have digestive problems resulting from gluten in wheat has also increased. Teff has an attractive nutritional profile, as it not only gluten-free but also high in dietary fiber, protein, iron, and calcium. Seven samples were prepared for this study. The quality characteristics of gluten-free noodles were evaluated based on pH, salinity, water absorption, turbidity, color, texture properties, tensile strength, and SEM. The pH value was the highest in TF100 with a pH of 6.66 and the lowest in the control with a pH of 6.42. Salinity showed no significant difference among all samples, and it ranged from 0.02~0.04% (p<0.05). Water absorption was the highest in TFX with a value of 66.11%, and the lowest in the control with a value of 44.81%. Turbidity showed no significant difference among all samples, and it ranged from 0.14~0.21 O.D. (p<0.05). While the lightness and yellowness values decreased with an increase in teff flour content, the redness value tended to decrease. The color difference value was the highest in the sample group without gluten. Based on the texture profile analysis, the hardness was highest in the control with a value of 46.74 N and lowest in TF100 with a value of 18.34 N. The springiness showed no significant difference among all samples. The cohesiveness was highest in the control with a value of 0.92 N. The chewiness decreased with an increase in teff flour content. Although the control with gluten had the highest tensile strength at $3.42kg/cm^2$, TFX had considerable tensile strength at $2.30kg/cm^2$. This study demonstrated the processability of gluten-free noodles using teff flour.

• 폴리머
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• 제38권2호
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• pp.156-163
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• 2014

하이드록시아파타이트는 골 전도가 우수하고 생체 적합성이 우수하며 염증 반응을 일으키지 않아 임상에서 골이식재로 널리 사용되고 있다. 본 연구에서는 하이드록시아파타이트를 함유한 poly(lactic-co-glycolic acid) (PLGA) 지지체를 제조하였으며 생체 내/외의 실험을 통하여 골 이식재로서의 응용가능성을 평가하였다. 하이드록시아파타이트/PLGA 지지체는 0, 10, 20, 40 및 60 wt%의 함량으로 제조하였다. 기계적 특성을 알아보기 위하여 압축강도, SEM, FTIR을 측정하였으며 MTT, RT-PCR, FACS, 조직학적 염색(H&E, ED-1)을 실시하였다. 그 결과 하이드록시아파타이트를 함유한 PLGA 지지체에서 염증 반응이 감소하는 것을 확인할 수 있었으며 골 이식재로서의 가능성을 보여주었다.

• 한국자원식물학회지
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• 제34권4호
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• pp.278-286
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• 2021

유전자 가위 기술 등 생명공학 기술을 콩에 적용하여 새로운 품종을 개발하기 위해서는 효율적인 조직배양 기술이 필수적이다. 식물의 유전형은 조직배양 효율에 의존하는 형질전환 기술의 성공 여부를 결정짓는 중요한 요소로 알려져 있다. 본 연구에서는 우리나라 콩 핵심 집단 내 21개 자원들을 선발하여, 외래 품종 2종과 함께 조직배양 효율을 조사하였다. 그 결과, 근연 관계가 높은 Kwangan, Anpyeong, Seonam은 발아율과 재분화 효율이 높았으며, Daepung, Daewon 품종은 발아율과 재분화율 모두 낮게 관찰되었다. 또한 3종의 외래 품종에서는 표준 유전체 해독에 사용된 Williams82와 Jack, Maverick 모두 높은 조직배양 효율을 보였다. 조직배양 효율이 높은 자원들을 대상으로 Agrobacterium법에 의한 형질전환을 수행하여 PCR 및 bar-strip 분석한 결과 Kwangan, Pungwon, Seonam, 그리고 Maverick 품종에서 제초제 저항성 유전자의 삽입을 확인할 수 있었다. 이들 결과를 바탕으로 농업적 가치가 높은 다양한 콩 품종들의 형질전환을 통한 새로운 품종 개발이 가능할 것이다.

• 한국건설순환자원학회논문집
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• 제9권4호
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• pp.553-560
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• 2021

본 연구에서는 순수시료를 사용한 시멘트 클링커와 석탄재를 7% 적용한 클링커를 1050~1500℃의 온도로 소성하여 소성온도별 시멘트 광물의 함량 변화와 미세구조의 변화를 검토하였다. 시멘트 클링커의 원료로서 석탄재의 적용은 알루미나 및 규산질의 공급원으로 적용이 가능하였으며, 석탄재를 적용한 시멘트 클링커는 1350℃ 이상의 소성온도에서 순수원료를 적용한 시멘트 클링커와 동등 수준의 광물량을 나타내었고, 미세구조 또한 유사한 상태를 나타내어 시멘트 원료로서 석탄재의 활용이 가능함을 확인하였다. XRD-Reitveld 분석에서 1250℃에서 Belite의 최대량이 생성되어 1350℃에서부터 Belite에서 Alite로의 전환이 나타났다. 1350℃에서부터 간극상과 Alite로 추정되는 광물상이 구분되며, 1400℃에서부터 명확하게 구분되었다. 소성온도가 증가함에 따라 결정상의 형상과 경계가 명확해지며, 결정상의 크기도 증가되는 것이 나타났다.

• Journal of Animal Science and Technology
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• 제63권6호
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• pp.1314-1327
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• 2021

Bacillus is characterized by the formation of spores in harsh environments, which makes it suitable for use as a probiotic for feed because of thermostability and high survival rate, even under long-term storage. This study was conducted to investigate the effects of Bacillus-based probiotics on growth performance, nutrient digestibility, intestinal morphology, immune response, and intestinal microbiota of weaned pigs. A total of 40 weaned pigs (7.01 ± 0.86 kg body weight [BW]; 28 d old) were randomly assigned to two treatments (4 pigs/pen; 5 replicates/treatment) in a randomized complete block design (block = BW and sex). The dietary treatment was either a typical nursery diet based on corn and soybean meal (CON) or CON supplemented with 0.01% probiotics containing a mixture of Bacillus subtilis and Bacillus licheniformis (PRO). Fecal samples were collected daily by rectal palpation for the last 3 days after a 4-day adaptation. Blood, ileal digesta, and intestinal tissue samples were collected from one pig in each pen at the respective time points. The PRO group did not affect the feed efficiency, but the average daily gain was significantly improved (p < 0.05). The PRO group showed a trend of improved crude protein digestibility (p < 0.10). The serum transforming growth factor-β1 level tended to be higher (p < 0.10) in the PRO group on days 7 and 14. There was no difference in phylum level of the intestinal microbiota, but there were differences in genus composition and proportions. However, β-diversity analysis showed no statistical differences between the CON and the PRO groups. Taken together, Bacillus-based probiotics had beneficial effects on the growth performance, immune system, and intestinal microbiota of weaned pigs, suggesting that Bacillus can be utilized as a functional probiotic for weaned pigs.

• 우울조울병
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• 제16권3호
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• pp.158-162
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• 2018

Objectives : The purpose of this study was to examine psychosocial factors related to burnout of social welfare officers working in Jeonnam Province. Methods : A total of 395 social welfare officers (male 99, female 296) working in 22 areas of Jeollanam-do province, were subjects of this study. We examined socio-demographic factors, using a self-reporting questionnaire. Subjects were asked to complete the Maslach Burnout Inventory-General Survey (MBI-GS), Center for Epidemiological Studies Depression Scale (CES-D), Perceived Stress Scale (PSS), and the Generalized Self-Efficacy Scale (GSS), to assess psychosocial factors affecting to burnout of social welfare officers. Results : Among 395 subjects, 221 (55.9%) reported recent experiences of burnout. There was no significant difference in age between two groups, divided by burnout. Sex (p<0.001), rank (p=0.003), working period (p=0.034), depression (p<0.001) revealed differences between the burnout group and control group. Scores of PSS (p<0.001) were higher, while the scores of GSS (p<0.001) were lower in the burnout group, than control group. Multivariate logistic regression analysis revealed that female (OR 2.840, 95%CI 1.466-5.504, p=0.002), depressive high-risk group (OR 6.824, 95%CI 2.893-16.096, p<0.001) PSS (OR 1.247, 95%CI 1.153-1.349, p<0.001) and GSS (OR 0.950, 95%CI 0.930-0.971, p<0.001), were significantly associated with burnout. Conclusion : We found that some factors, were associated with experienced burnout in social welfare officers. Depressive symptoms were the strongest associative factor, for burnout in public servants in charge of social welfare. Sex, stress and self-efficacy also correlated with burnout, and especially self-efficacy was a protecting factor.

• 국제지역연구
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• 제17권4호
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• pp.155-179
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• 2013

본 연구의 목적은 남북 수단 분쟁의 역사와 과정을 고찰하여 남북 수단 분쟁을 이해하는데 있다. 이를 위해 분쟁원인과 해결에 대한 이론과 실제적 접근을 통해 분쟁원인과 해법의 일반론적인 검토와 더불어 남북 수단의 특별한 상황을 이해하고자 하였다. 이후 남북수단 분쟁의 역사적 배경 및 원인을 19세기 이전의 남북수단, 이집트의 수단 정복과 남북의 이질화, 영국의 식민지배와 남북 갈등 심화, 영국의 식민지배와 남북 갈등 심화, 수단의 독립에 따른 남북 수단 갈등, 종교와 자원을 둘러싼 남북의 갈등 등으로 구분해서 살펴보았다. 다음 장에서는 남북 수단 분쟁의 경과를 제1, 2차 내전으로 나누어 분석하였다. 남북 수단 분쟁은 이집트 영국의 식민지배 기간 중 남부와 북부의 분리통치 정책으로 인해 이질화가 심화되었고, 게다가 인종 종교 등 고질적 요소와 석유 이권을 둘러싼 갈등 등이 원인이었다. 2005년 합의된 포괄적 평화협정이 이행되어 남수단이 국민투표를 통해 분리 독립했다. 수단은 내전이 합의와 협정을 통해 해결된 아프리카 분쟁의 대표적 사례로 기록되고 있다. 이는 당시 전문가들이 수단 중앙정부가 2011년 분리 독립을 승인하지 않을 것이라는 전망을 내놓았던 것을 볼 때도 매우 이례적인 일로 받아들여지고 있다. 이제 수단은 남수단이 분리 독립하면서 새로운 전기를 맞이하고 있다. 그러나 아직까지 국경지역 일부와 유전이 개발되고 있는 몇몇 곳에서는 갈등과 충돌이 계속되고 있다. 특히 아비에이 지역을 둘러싸고 수단과 남수단은 합의점을 찾지 못하고 있다. 남북 수단분쟁은 아프리카 연합의 중재로 잠정 해결되었지만 근본적인 문제는 논란이 되고 있어 추가적인 합의가 필요한 시점이다. 양측과 국제사회의 합리적인 해법이 요구되고 있다.