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Effect of Cryopreservation of Sibling 2PN Zygotes on Cumulative Delivery Rates in the Human IVF-ET Program (전핵 시기에서의 수정란 동결이 체외수정 및 배아이식술에서의 누적 분만율에 미치는 영향)

  • Kim, Myo-Kyung;Lee, Sun-Hee;Choi, Su-Jin;Choi, Hye-Won;Park, Dong-Wook;Lim, Chun-Kyu;Song, In-Ok;Lee, Hyoung-Song
    • Clinical and Experimental Reproductive Medicine
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    • v.37 no.4
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    • pp.329-338
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    • 2010
  • Objective: This study was carried out to know whether cryopreservation of sibling 2PN zygotes could increase the cumulative delivery rates in the patients who had less than 10 fertilized zygotes. Methods: A retrospective analysis was performed in 138 in vitro fertilization-embryo transfer (IVF-ET) cycles with less than 10 fertilized zygotes during January 2003 to December 2007 in Cheil General Hospital. These cycles were divided into two groups. In Group I (n=86), all fertilized embryos were cultured to transfer on day 3 without cryopreserved embryos at the 2PN stage. In Group II (n=52), among fertilized zygotes, some sibling zygotes were frozen at the 2PN stage, the remainder were cultured to transfer. Clinical outcomes in fresh ET cycles and cumulative ongoing pregnancy rates after subsequent frozen-thawed (FT)-ET cycles were compared. Results: There were no significant differences in female mean age, number of retrieved oocytes and total fertilized embryos between two groups, Number of cultured embryos was significantly lower in Group II ($5.2{\pm}0.5$) than in Group I ($8.4{\pm}0.7$) (p<0.01). Also, number of transferred embryos was significantly lower in Group II ($3.3{\pm}0.6$) compared with Group I ($3.6{\pm}0.6$) (p<0.01). ${\beta}$-hCG positive rates and delivery rates (51.2 vs. 46.2 % and 41.9 vs. 34.6 %, respectively) after fresh ET were slightly higher in Group I than in Group II. However, the differences were not statistically significant. Also, the cumulative delivery rates after subsequent FT-ET cycles were not significantly different between Group I (48.8%) and Group II (50.0%). Conclusion: This study showed that cryopreservation of sibling 2PN zygotes from patients who had less than 10 zygotes in the fresh ET cycles did not increase cumulative delivery outcomes. But, it could provide an alternative choice for patients due to offering more chance for embryo transfers if pregnancy was failed in fresh IVF-ET cycles.

Selection of Pure Lines with Various Growth and Flowering Characteristics of Spreading Petunia, Petunia × hybrida (생육 및 개화 특성이 다양한 덩굴 페튜니아 순계 선발)

  • Song, Cheon Young
    • FLOWER RESEARCH JOURNAL
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    • v.17 no.2
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    • pp.128-136
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    • 2009
  • To obtain pure lines for breeding $F_1$ varieties of spreading surfinia and wave petunia, Petunia ${\times}$ hybrida, 20 lines of surfinia petunia and 28 lines of wave petunia that were considered uniform in growth and flowering characteristics were selected by self-pollination of the fifth($S_5$) or the seventh generation($S_7$). The 20 selected lines of surfinia petunia had the branch number ranged from 6.0 to 11.0 cm, and the internode length ranged from 2.0 to 4.2 cm. Among them, ten lines, including '$Pe99-017^7$' were above 60 cm of plant width, above 300 leaves in a plant. Fourteen lines including '$Pe99-017^7$' were more than 150 in the number of flower. In the petal color, thirteen lines, including '$Pe99-017^7$', were red-purple; three, including '$Pe99-007^7$', were purple; '$Pe04-086^7$' and '$Pe04-159^7$' were violet; and line '$Pe072-1^7$' was white. Eight lines including '$Pe02-205-2^5$' ranged from 4.0 to 5.0 cm of flowers diameter, and seven lines including '$Pe04-086^5$' ranged from 3.0 to 4.0 cm of leaf length, which is relatively low. Germination rate of the lines was more than 50%. In the wave petunia, the branch number of the 28 selected lines ranged from 8.0 to 13.0 cm, and the internode length ranged from 1.0 to 3.2 cm, which is relatively higher than surfinia petunias. Among them, ten lines, including '$Pe99-020^7$' were above 60 cm of plant width, above 200 leaves in a plant. Twelve lines including '$Pe04-034-2^5$' were more than 150 in the number of flower. In the petal color, eighteen lines, including '$Pe99-020^7$', were red-purple; three, including '$Pe04-113-4^5$', were red; three, including '$Pe04-263^5$', were white; '$Pe04-201^5$' and '$Pe04-263^5$' were violet blue; and line '$Pe04-072-5^5$' was purple. Nine lines including '$Pe04-201^5$' ranged from 4.0 to 5.0 cm of flowers diameter, and eleven lines including '$Pe04-263^5$' ranged from 3.0 to 4.0 cm of leaf length, which is relatively low. All the lines with various growth and flowering characteristics would be very promising to use as breeding materials for $F_1$ hybrids of spreading petunia, Petunia ${\times}$ hybrida.

A Study on The Thickness Shrinkage of Injection Molded Parts with The Variation of Injection Mold Core and Molding Materials (사출금형코어 및 성형수지 변화에 따른 두께 방향 수축률에 관한 연구)

  • Shin, Sung-Hyun;Jeong, Eui-Chul;Kim, Mi-Ae;Chae, Bo-Hye;Son, Jung-Eon;Kim, Sang-Yoon;Yoon, Kyung-Hwan;Lee, Sung-Hee
    • Design & Manufacturing
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    • v.13 no.2
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    • pp.17-21
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    • 2019
  • In this study, selective laser sintered 3D printing mold core and metal core were used to investigate the difference of the thickness shrinkage from the gate of the injection molded part at a constant interval. SLS 3D printing mold core was made of nylon-based PA2200 powder and the metal core was manufactured by conventional machining method. As the PA2200 powder material has low strength, thermal conductivity and high specific heat characteristics compared with metal, molding conditions were set with the consideration of molten temperature and injection pressure. Crystalline resin(PP) and amorphous resin(PS) with low melting temperature and viscosity were selected for the injection molding experiment. Cooling time for processing condition was selected by checking the temperature change of the cores with a cavity temperature sensor. The cooling time of the 3D printing core was required a longer time than that of the metal core. The thickness shrinkage of the molded part compared to the core depth was measured from the gate by a constant interval. It was shown that the thickness shrinkage of the 3D printing core was 2.02 ~ 4.34% larger than that of metal core. In additions, in the case of metal core, thickness shrinkage was increased with distance from the gate, on the contrary, in the case of polymer core showed reversed aspect.

A Study on the Usefulness of Characteristic Past History Investigation for Life Care in People with Lumbar Instability (허리부위 불안정성자 라이프케어를 위한 특징적과거력 조사의 유용성에 관한 연구)

  • Ki, Chul;Heo, Myoung;Song, Seong-Min
    • Journal of Korea Entertainment Industry Association
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    • v.13 no.8
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    • pp.583-593
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    • 2019
  • The purpose of this study was to investigate the relation between and subjective instability pain behavior (SIPB) and physical instability test(PIT) according to the presence of characteristic past histories(CPH) in people with chronic low back pain(CLBP). Forty CLBP subjects participated in this study. The presence of four characteristics past histories(long term history, traumatic experience, sports activities, neurologic sign) were examined. According to presence number(PN) of CPH, subjects were divided into 5 groups[group 1(PN:0): n=8, group 2(PN:1): n=8, group 3(PN:2): n=8, group 4(PN:3): n=8, group 5(PN:4): n=8]. After 16 items were examined for the SIPBs, then Seven PITs were conducted, and the results were scored. The SIPBs and PITs were compared according to the presence numbers of CPH, and the relation between them was analyzed. There was a significant difference(p<.05) in both SIPB scores and PIT scores in the comparison of groups according to the presence number of CPH. There was high positive correlation between the presence numbers of CPH and SIPB score(r=.819, p=.000) and PIT score(r=.606, p=.000). Also, there was a correlation between SIPB score and PIT score(r=.571, p=.000). Based on the findings in the present study, the presence of three or more CPH in people with CLBP may be a useful variable in the diagnosis of lumbar instability. The combined findings of the three variables such as CPH, SIPB, and PIT can improve the accuracy of lumbar instability diagnosis.

The Study on Automatic Speech Recognizer Utilizing Mobile Platform on Korean EFL Learners' Pronunciation Development (자동음성인식 기술을 이용한 모바일 기반 발음 교수법과 영어 학습자의 발음 향상에 관한 연구)

  • Park, A Young
    • Journal of Digital Contents Society
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    • v.18 no.6
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    • pp.1101-1107
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    • 2017
  • This study explored the effect of ASR-based pronunciation instruction, using a mobile platform, on EFL learners' pronunciation development. Particularly, this quasi-experimental study focused on whether using mobile ASR, which provides voice-to-text feedback, can enhance the perception and production of target English consonants minimal pairs (V-B, R-L, and G-Z) of Korean EFL learners. Three intact classes of 117 Korean university students were assigned to three groups: a) ASR Group: ASR-based pronunciation instruction providing textual feedback by the mobile ASR; b) Conventional Group: conventional face-to-face pronunciation instruction providing individual oral feedback by the instructor; and the c) Hybrid Group: ASR-based pronunciation instruction plus conventional pronunciation instruction. The ANCOVA results showed that the adjusted mean score for pronunciation production post-test on the Hybrid instruction group (M=82.71, SD =3.3) was significantly higher than the Conventional group (M=62.6, SD =4.05) (p<.05).

Variation of nitric oxide concentrations in response to shaking stress in the Manila clam Ruditapes philippinarum (일산화질소 (nitric oxide) 정량을 통한 바지락(Ruditapes philippinarum) 의 흔들림 스트레스 측정)

  • Park, Kyung-Il
    • The Korean Journal of Malacology
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    • v.29 no.1
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    • pp.1-6
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    • 2013
  • This study aimed to evaluate the effects of shaking stress in the hemolymph of the Manila clam Ruditapes philippinarum by quantification of nitric oxide (NO) levels. The clams were divided into 3 groups as follows: clams placed in a plain container (control), clams injected with nitro-L-arginine methyl ester (NAME, an NO inhibitor), and clams in a container filled with nylon fiber at a density of $1kg/m^3$. Subsequently, each group was placed in sea water and shaken at 100 rpm for 6 h. The concentration of NO was quantified by using DAF assay and Griess assay. Both the assays showed that while shaking significantly increased the NO concentration, the NO inhibitor reduced the NO concentration in the hemolymph of the clams tested. In addition, the nylon fiber, which was used as a filler, effectively prevented the increase in NO concentration. This result suggests that measurement of NO concentration is a useful tool for evaluation of physiological stress in marine bivalves. In addition, it should be considered that a filler is necessary when dredge fishing or the suspended clam culture method is developed.

Morphological and Phylogenetic Characteristics of Nematophagous Fungi (식물기생성 선충 포식곰팡이의 형태 및 계통분류학적 특성)

  • Kang, Doo-Sun;Jeon, Han-Ki;Son, Hee-Seong;Whang, Kyung-Sook;Cho, Cheon-Whi
    • Applied Biological Chemistry
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    • v.50 no.2
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    • pp.101-106
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    • 2007
  • Twenty-two strains of nematophagous fungi were isolated from 100 soil samples. Nematophagous fungi were classified into three categories; 3-dimensional adhesive nets (A group), 2-dimensional adhesive nets (B group) and constricting ring (C group). Nine strains were selected and identified on the basis of morphological characteristics (hypha, conidiophore, form and size of conidia, number of conidia, node of conidophore, number and location of septa, size and color of chlamydospore) and ITS (internal transcribed spacer) region of rDNA sequences. As the results, the isolated were identified as belonging to the species of Monacrosporium thaumasium (Kan-2, Kan-4, Kan-11), Arthrobotrys oligospora (Kan-9, Kan-13, Kan-20, Kan-21), A. musiformis (Kan-12), and A. dactyloides (Kan-22).

Elementary School Students' Level of Computational Thinking through Bebras Challenge 2017 (비버 챌린지 2017을 통한 초등학생들의 컴퓨팅 사고 수준 분석)

  • Jun, Soojin;Jeon, Yongju;Kim, Seulgi;Kim, Doyong;Jeong, Inkee
    • Journal of The Korean Association of Information Education
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    • v.22 no.3
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    • pp.345-356
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    • 2018
  • The purpose of this paper is to analyze the elementary school students' level of computational thinking ability by analyzing the domestic question and examination results of Beaver Challenge 2017. Based on the results of the 'Challenge' results of Beaver Challenge 2017, the achievement by element, gender, and grade of elementary school students II (elementary school 3.4) and group III (elementary school 5.6) Respectively. As a result of the analysis, the achievement by the evaluation factors showed difference within each group. In addition, there was no significant difference in achievement according to gender except for one area of group II. The achievement level of each grade was statistically significant in the group III. The results of this study are expected to provide implications for the level of students related to what can be evaluated in elementary school information education and contribute to the follow-up research.

Spawning Season, and Factors Influencing Allometric Growth Pattern and Body Condition of Walleye Pollock Gadus chalcogrammus in the Middle East Sea, Korea (동해 중부 연안에 출현하는 명태(Gadus chalcogrammus)의 산란기 및 성장패턴(allometric growth pattern)과 비만도(body condition) 변화에 영향을 미치는 요인)

  • Lee, Chung Il;Han, Moon Hee;Jung, Hae Kun;Park, Hyun Je;Park, Joo Myun
    • Korean Journal of Ichthyology
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    • v.31 no.3
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    • pp.141-149
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    • 2019
  • This study presents data on spawning season, weight-length relationships (WLRs) and condition factor of Gadus chalcogrammus inhabiting the middle East Sea, Korea. Monthly changes in the gonadosomatic index (GSI) revealed that the spawning period was from December to March. Overall value of the exponent b, estimated by nonlinear least squares from weight and length data was 2.806, ranging from 2.778 for female fishes to 2.985 for immature group. The b values were similar between spawning and non-spawning, and between female and male fishes, but it was significantly higher for immature than mature fishes. The condition factors were significantly higher for immature than mature groups, and during non-spawning than spawning periods, but it was not significant between male and female fishes.

Comparative Agricultural Traits of Soybean Germplasm from East Asia

  • Yu-Mi Choi;Hyemyeong Yoon;Sukyeung Lee;Myoung-Jae Shin;Myung-Chul Lee;Joungyun Yi;On sook Hur;Na young Ro;Kebede Taye Desta
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2020.12a
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    • pp.51-51
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    • 2020
  • 콩의 원산지는 만주를 중심으로한 동북아시아를 원산지로 추정하고 있으며, 동아시아인 한국, 중국, 일본의 주요 식재료로 다양하게 이용되어왔다. 콩은 단백질과 지방의 공급원으로서 뿐 아니라 최근에는 콩의 생리활성 물질이 항암효과, 골다공증, 신장질환과 같은 질병예방에도 효과가 있는 것으로 알려지면서 활용가능성이 높아지고 있다. 본 연구는 유전자원센터에 보존 중인 콩 수집단에서 농업형질, 기초정보 등을 토대로 핵심집단을 작성하고 그 중 한국, 중국, 일본 수집단 각 425, 93, 42자원의 개화일수, 생육일수, 주당협수, 100립중, 주당수량과 같은 양적형질을 비교하였다. 개화일수와 생육일수는 중국수집 자원에서 평균38일, 107일로 가장 빠르며, 한국수집 자원은 평균 53일, 127일로 가장 늦었다. 협당립수는 중국수집 자원에서 협당 평균3립으로 가장 많았으며, 한국과 일본 수집자원은 각 2.3과 2.4립으로 유사하였다. 주당협수는 한국 수집자원에서 주당 평균 327.4개로 가장 많고 일본이 248.0개 중국이 224.4순으로 조사되었다. 100립중은 일본 수집 자원이 평균 33.6g으로 대립이 많고 한국 26.5g , 중국 21.1g 이었다. 주당수량은 한국 수집자원이 평균 119.8g, 일본 84.2g, 중국 76.8g순이었다. 이들 5개 양적형질을 토대로 군집분석한 결과 한국 자원은 대다수가 C3 그룹에 속하고, C1 그룹에 일부가 속하였으며, 일본 자원은 C3 그룹과 C2 그룹에, 중국 자원은 C2 그룹에 속하여 한국과 일본자원이 유사하고 중국자원과는 차이를 보이는 것으로 나타났다. 이러한 특성조사 결과는 자원의 수집, 도입시 다양성 확보를 위한 기초정보로 이용할 수 있다.

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