Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Morphological and Phylogenetic Characteristics of Nematophagous Fungi

식물기생성 선충 포식곰팡이의 형태 및 계통분류학적 특성

  • Kang, Doo-Sun (Korea Agriculture Fertilizer Corporation Biochemistry Research Institute) ;
  • Jeon, Han-Ki (Korea Agriculture Fertilizer Corporation Biochemistry Research Institute) ;
  • Son, Hee-Seong (Institute of Microbial Ecology and Resources, Mokwon University) ;
  • Whang, Kyung-Sook (Institute of Microbial Ecology and Resources, Mokwon University) ;
  • Cho, Cheon-Whi (Department of Bio-Environmental Chemistry, Chungnam National University)
  • 강두선 (KAFCO 생물화학연구소) ;
  • 전한기 (KAFCO 생물화학연구소) ;
  • 손희성 (목원대학교 미생물생태자원연구소) ;
  • 황경숙 (목원대학교 미생물생태자원연구소) ;
  • 조천휘 (충남대학교 생물환경화학전공)
  • Published : 2007.06.30
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Abstract

Twenty-two strains of nematophagous fungi were isolated from 100 soil samples. Nematophagous fungi were classified into three categories; 3-dimensional adhesive nets (A group), 2-dimensional adhesive nets (B group) and constricting ring (C group). Nine strains were selected and identified on the basis of morphological characteristics (hypha, conidiophore, form and size of conidia, number of conidia, node of conidophore, number and location of septa, size and color of chlamydospore) and ITS (internal transcribed spacer) region of rDNA sequences. As the results, the isolated were identified as belonging to the species of Monacrosporium thaumasium (Kan-2, Kan-4, Kan-11), Arthrobotrys oligospora (Kan-9, Kan-13, Kan-20, Kan-21), A. musiformis (Kan-12), and A. dactyloides (Kan-22).

국내 다양한 토양시료로부터 우수 선충포식곰팡이 9 균주를 선발하였다. 순수분리된 선충포식곰팡이는 포식기관의 형태에 따라 3차원적 점착성 그물구조(3-dimensional adhesive nets)를 나타내는 선충포식곰팡이(A 그룹), 2차원적 점착성 그물구조(2-dimensional adhesive nets)를 나타내는 선충포식곰팡이(B 그룹)와 수축성 고리구조(constricting ring)를 나타내는 선충포식곰팡이(C 그룹)로 크게 3개의 형태그룹으로 분류되었다. 이들 각 그룹에 속하는 선충포식곰팡이의 균사체, 분생포자병, 분생포자의 모양과 크기, 분생포자 형성 개수, 분생포자 마디(node), 분생포자 격막의 수와 격막의 위치, 휴면포자의 형성과 크기 및 색 등 형태학적 특징과 선충포식곰팡이의 rDNA ITS 영역을 PCR로 증폭하여 염기서열을 분석한 결과, 분리된 포식곰팡이는 Monacrosporium thaumasium(Kan-2, Kan-4, Kan-11), Arthrobotrys oligospora(Kan-9, Kan-13, Kan-20, Kan-21), A. musiformis (Kan-12), A. dactyloides(Kan-22)로 동정되었다.

Keywords

References

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