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Effect of Medium Compositions on the Mycelial Growth of Inonotus obliquus (차가버섯의 균사체 성장에 대한 배지성분의 영향)

  • Choi, Keun Ho
    • Korean Chemical Engineering Research
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    • v.43 no.3
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    • pp.419-424
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    • 2005
  • Effect of temperature($22-32^{\circ}C$), pH(5-7) and medium composition on the mycelial growth for the submerged culture of Inonotus obliquus. The concentrations of glucose, starch, peptone, yeast extract, $K_2HPO_4$, $MgSO_4{\cdot}7H_2O$ and $CaCl_2$ were examined in the ranges of 30-120 g/L, 0-10 g/L, 0-20 g/L, 0-15 g/L, 0-2 g/L, 0-1.5 g/L and 0-0.5g/L, respectively. The maximum mycelial growth of Inonotus obliquus was obtained for $26-27^{\circ}C$ and pH 6. The concentrations of glucose, yeast extract and $CaCl_2$, which gave the maximum mycelial growth of Inonotus obliquus, were 70 g/L, 5 g/L and 0.1 g/L, respectively. In the cases of starch, peptone and $K_2HPO_4$, the mycelial growth of Inonotus obliquus increased with increasing the concentrations. However, as the concentration of $MgSO_4{\cdot}7H_2O$ increased, the mycelial growth of Inonotus obliquus decreased. The medium for maximum mycelial growth of Inonotus obliquus consisted of (per 1 L): glucose, 70 g; peptone, 5-20 g; starch, 10 g; yeast extract, 5 g; $K_2HPO_4$, 2 g and $CaCl_2$, 0.1 g.

AN IMMUNOHISTOCHEMICAL STUDY ON THE IMMUNOGLOBULIN G SUBCLASSES OF THE EXPERIMENTALLY INDUCED RAT PULP AND PERIAPICAL PATHOSES (실험적 백서 치수 및 치근단 병소에서의 면역글로불린 G 아강분포에 관한 면역조직화학적 연구)

  • Baek, Seung-Ho;Lim, Sung-Sam
    • Restorative Dentistry and Endodontics
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    • v.16 no.1
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    • pp.41-59
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    • 1991
  • This study was performed to elucidate the distribution and correlation of immunoglobulin G subclasses with the degree of inflammation in the experimentally induced rat pulp and periapical pathoses. The pulp exposures were made in 108 mandibular 1st molars of 54 rats and the teeth were left open to the oral environment The animals were sacrified at 3, 7, 15, 30, 60 and 90 days after pulp exposure, and examined microscopically and radiographically Seventy one specimens were routinely sectioned at the thickness of 4 - $6{\mu}$ and stained with Hematoxylin - eosin for histologic examination, with toluidine blue for mast cells, and with the primary antibodies against rat IgG subclasses by using the Avidin - Biotin complex method. The following results were obtained: 1. As the degree of inflammation of rat pulp and periapeces intensified, the number of IgG subclass containing cells per unit area, especially IgG2a and IgG2c, decresased. 2. The IgG2c cells were most predominantly found in the lesions with slight inflammation, IgG1 cells in mild or severe inflammation, and IgG2a cells in moderate inflammation. 3. IgG subclass containg cells were more predominantly observed in the periapical granuloma than periapical abscess or cyst(p<0.01). 4. IgG2a containing cells were predominant in pulp inflammation, IgG1 containing cells in periapical granuloma, IgG2a cells and IgG1 cells in periapical abscess, and IgG2a cells were significantly predominant in periapical cyst. 5. The number of IgG subclass containing cells and mast cells in periapical tissue decreased with time lapse after pulp exposure. And correlation index between mast cells and IgG1, IgG2a, IgG2b was stastically high.

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Expanding Generalized Hadamard Matrices over $G^m$ by Substituting Several Generalized Hadamard Matrices over G

  • No, Jong-Seon;Song, Hong-Yeop
    • Journal of Communications and Networks
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    • v.3 no.4
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    • pp.361-364
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    • 2001
  • Over an additive abelian group G of order g and for a given positive integer $\lambda$, a generalized Hadamard matrix GH(g, $\lambda$) is defined as a gλ$\times$gλ matrix[h(i, j)], where 1 $\leq i \leqg\lambda and 1 \leqj \leqg\lambda$, such that every element of G appears exactly $\lambd$atimes in the list h($i_1, 1) -h(i_2, 1), h(i_1, 2)-h(i_2, 2), …, h(i_1, g\lambda) -h(i_2, g\lambda), for any i_1\neqi_2$. In this paper, we propose a new method of expanding a GH(g^m, \lambda_1) = B = [B_{ij}] over G^m$ by replacing each of its m-tuple B_{ij} with B_{ij} + GH(g, $\lambda_2) where m = g\lambda_2. We may use g^m/\lambda_1 (not necessarily all distinct) GH(g, \lambda_2$)s for the substitution and the resulting matrix is defined over the group of order g.

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The IgG subclass responses in the phenotypic subsets of the early-onset periodontitis (조기발병형 치주염의 표현형적 소집단의 IgG Subclass에 대한 연구)

  • Choi, Jeom-Il
    • Journal of Periodontal and Implant Science
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    • v.29 no.1
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    • pp.251-264
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    • 1999
  • 본 연구는 조기발병형 치주염의 서로 다른 4가지 표현형에 있어서 Porphyromonas gingivalis(Pg) 381과 Actinobacillus actinomycetemcomitans(Aa) Y4에 대한 상승된 IgG subclass의 양상을 평가하기 위해 시행하였다. Subform I(distinctive localized juvenile periodontitis pattern)에서 3명 subform II(post juvenile periodontitis pattern)에서 19명, subform III (localized but rapidly progressing pattern)에서 15명, subform IV(distinctive rapidly progressing periodontitis pattern)에서 15명의 환자를 조사하여 Pg에 대한 그들의 total IgG level과 각각의 IgG subclass level 및 Aa에 대한 IgG level을 검사했다. Pg에 대한 total IgG level은 subform II와 IV보다 subform I과 III에서 훨씬 높게 나타났다. IgG3 level이 subform I과 IV사이에서 현저한 차이가 있다는 점을 제외하고는, 다른 IgG subclass level에서 subform 사이에 아무런 차이가 없었다. Pg에 대한 IgG subclass는 single class 혹은 다양한 group에서 상승되어 나타났으며, IgG1+2+4가 가장 흔하게 발견되었고, 다음으로 IgG4 단독, IgG2 단독, IgG2+4, IgG2+3+4의 순으로 발견되었다. IgG2와 IgG4가 빈번히 상승되어 발견되었는데, 특히 severe form(subform III & IV)에서 그러했다. 뿐만 아니라, IgG level은 subform II, III, IV와 일치하여 점차적으로 증가하였고, 반면에 IgG1/IgG4 ratio는 그와 일치하여 감소되었다. 이러한 ratio의 감소는 단백질성의 오래된 항원의 과부하로 인해 immunoglobulin gene의 전환을 가능하게 한다는 것을 나타내고 있다. Aa에 대한 IgG2 level은 다른 유형보다 subform I에서 상당히 높았다. Pg에 대한 IgG2 levels이 subform I의 국소 부위에서 발생하는 disease activity와 밀접한 관련이 있으며, Aa의 경우에는 이러한 관련성이 나타나지 않았다. Pg에 대한 IgG2 level은 18-25세에서 훨씬 높은 동시에 26-35세에서는 감소했으며 결국 30대 후반에서는 더 높은 수치로 되돌아갔다. 이러한 결과는 Pg에 대한 IgG2 및 IgG responsiveness (single 혹은 combined)가 EOP의 severe form의 발달에 중요하게 작용하며 IgG2 levels은 IgG1/IgG4 ratio와 더불어 EOP의 localized type이 generalized type으로 계속 진행하는 것을 조절하는 역할을 하는 것으로 보인다는 것을 강하게 시사하였다.

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Isoforms of Glucose 6-Phosphate Dehydrogenase in Deinococcus radiophilus

  • Sung, Ji-Youn;Lee, Young-Nam
    • Journal of Microbiology
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    • v.45 no.4
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    • pp.318-325
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    • 2007
  • Glucose 6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49) in Deinococcus radiophilus, an extraordinarily UV-resistant bacterium, was investigated to gain insight into its resistance as it was shown to be involved in a scavenging system of superoxide $(O_2^{-1})$ and peroxide $(O_2^{-2})$ generated by UV and oxidative stresses. D. radiophilus possesses two G6PDH isoforms: G6PDH-1 and G6PDH-2, both showing dual coenzyme specificity for NAD and NADP. Both enzymes were detected throughout the growth phase; however, the substantial increase in G6PDH-1 observed at stationary phase or as the results of external oxidative stress indicates that this enzyme is inducible under stressful environmental conditions. The G6PDH-1 and G6PDH-2 were purified 122- and 44-fold (using NADP as cofactor), respectively. The purified G6PDH-1 and G6PDH-2 had the specific activity of 2,890 and 1,033 U/mg protein (using NADP as cofactor) and 3,078 and 1,076 U/mg protein (using NAD as cofactor), respectively. The isoforms also evidenced distinct structures; G6PDH-1 was a tetramer of 35 kDa subunits, whereas G6PDH-2 was a dimer of 60kDa subunits. The pIs of G6PDH-1 and G6PDH-2 were 6.4 and 5.7, respectively. Both G6PDH-1 and G6PDH-2 were inhibited by both ATP and oleic acid, but G6PDH-1 was found to be more susceptible to oleic acid than G6PDH-2. The profound inhibition of both enzymes by ${\beta}-naphthoquinone-4-sulfonic$ acid suggests the involvement of lysine at their active sites. $Cu^{2+}$ was a potent inhibitor to G6PDH-2, but a lesser degree to G6PDH-1. Both G6PDH-1 and G6PDH-2 showed an optimum activity at pH 8.0 and $30^{\circ}C$.

Production of Polygalacturonase from Ganoderma lucidum (Ganoderma lucidum으로부터 Polygalacturonase의 생산)

  • Yoon, Sook;Kim, Myung-Kon;Hong, Jai-Sik;Kim, Myeong-Sook
    • The Korean Journal of Mycology
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    • v.22 no.4
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    • pp.286-297
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    • 1994
  • The optimum nutritional and cultural conditions of polygalacturonase by Ganoderma lucidum in liquid culture were studied. The optimal temperature, pH, and the duration of culture for production of the enzyme was $30^{\circ}C$, 5.5 and 14 days, respectively. The maximal production of the enzyme was obtained in a synthetic medium containing 10 g of pectin, 10 g of soluble starch, 1 g of yeast extract, 2 g of peptone, 1 g of phenylalanine, 2 g of $KH_2PO_4$, 0.2 g of $MgSO_4{\cdot}7H_2O$, 0.05 g of $CaCI_2$ and 100 g of $thiamin{\cdot}HCI$ in 1000 ml of distilled water.

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Topological Properties of Recursive Circulants : Disjoint Paths (재귀원형군의 위상 특성 : 서로소인 경로)

  • Park, Jeong-Heum;Jwa, Gyeong-Ryong
    • Journal of KIISE:Computer Systems and Theory
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    • v.26 no.8
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    • pp.1009-1023
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    • 1999
  • 이 논문은 재귀원형군 G(2^m , 2^k ) 그래프 이론적 관점에서 고찰하고 정점이 서로소인 경로에 관한 위상 특성을 제시한다. 재귀원형군은 1 에서 제안된 다중 컴퓨터의 연결망 구조이다. 재귀원형군 {{{{G(2^m , 2^k )의 서로 다른 두 노드 v와 w를 잇는 연결도 kappa(G)개의 서로소인 경로의 길이가 두 노드 사이의 거리d(v,w)나 혹은 G(2^m , 2^k )의 지름 \dia(G)에 비해서 얼마나 늘어나는지를 고려한다. 서로소인 경로를 재귀적으로 설계하는데, 그 길이는 k ge2일 때 d(v,w)+2^k-1과 \dia(G)+2^k-1의 최솟값 이하이고, k=1일 때 d(v,w)+3과 \dia(G)\+2의 최솟값 이하이다. 이 연구는 (2^m , 2^k )의 고장 감내 라우팅, 고장 지름이나 persistence의 분석에 이용할 수 있다.Abstract In this paper, we investigate recursive circulant G(2^m , 2^k ) from the graph theory point of view and present topological properties concerned with node-disjoint paths. Recursive circulant is an interconnection structure for multicomputer networks proposed in 1 . We consider the length increments of {{{{kappa(G)disjoint paths joining arbitrary two nodes v and win G(2^m , 2^k )compared with distance d(v,w)between the two nodes and diameter {{{{\dia(G)of G(2^m , 2^k ), where kappa(G)is the connectivity of G(2^m , 2^k ). We recursively construct disjoint paths of length less than or equal to the minimum of {{{{d(v,w)+2^k-1and \dia(G)+2^k-1for kge2 and the minimum of d(v,w)+3 and \dia(G)+2for k=1. This work can be applied to fault-tolerant routing and analysis of fault diameter and persistence of G(2^m , 2^k )

Effect of Controlled Release Fertilizer on the Growth and Flowering of Oncidium 'Sweet Sugar' (완효성비료 시비가 온시디움 생육과 개화에 미치는 영향)

  • Kim, Si Dong;Lee, Hee Doo;Kim, Ju Hyoung;Kim, Tae Jung
    • FLOWER RESEARCH JOURNAL
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    • v.17 no.4
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    • pp.246-250
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    • 2009
  • This study was conducted to determine the effect of controlled-release fertilizer on growth of Oncidium. Leaf and pseudobulb length increased with controlled-release fertilizer from 2 g to 3 g treatment compared to hyponex and controlled-release fertilizer I g treatment, while leaf width and number of leaf was not significant among treatments. Plant weight increased with controlled-release fertilizer from 2 g to 3 g treatment compared to hyponex and controlled-release fertilizer I g treatment. Flowering date(bloomed October early) did not show significant difference among treatments. The number of flowers showed the most in controlled-release fertilizer 3 g treatment as 62.4 ea/plant, but was not different significantly among treatments. Flower stem length and width were also non-significant among treatments. Branching numbers increased in the controlled-release fertilizer 2 g and 3 g treatments. Mineral elements of shoot increased from 2 g to 3 g treatment rather than the controlled-release fertilizer 1g treatment. Especially, K content was higher compared to N and P content. Therefore, appropriate controlled-release fertilizer amount for Oncidium was recommended as 2 g.

Emission of Airbone Pollutants from Traditional Korean Fireplace (아궁이 사용에 의한 대기오염물질의 배출 특성)

  • Park, Sung Kyu;Lyu, Kun Jung;Choi, Sang Jin;Kim, Dae keun;Kim, Dong Young;Jang, Young Kee
    • Journal of Climate Change Research
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    • v.6 no.2
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    • pp.113-119
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    • 2015
  • Emission from the traditional Korean fireplace, or the under-floor heating and cooking device, can contribute significantly to airborne pollutants inventories. This study has systematically measured emissions of airborne pollutants from the fireplace when used different fuels such as firewood, agricultural crop residuals, household wastes. The results show that emission factors of airborne pollutants through the primary combustion of firewood were 3.22 g/kg for TSP, 2.93 g/kg for $PM_{10}$, 2.65 g/kg for $PM_{2.5}$, 174.19 g/kg for CO, 7.77 g/kg for NO, 0.15 g/kg for $SO_2$, 40.53 g/kg for TVOC and 0.03 g/kg for $NH_3$; from burning of agricultural crop residues, 2.85 g/kg for TSP, 1.38 g/kg for $PM_{10}$, 1.14 g/kg for $PM_{2.5}$, 126.47 g/kg for CO, 12.60 g/kg for NO, 0.20 g/kg for $SO_2$, 33.73 g/kg for TVOC and 0.02 g/kg for $NH_3$; and for household wastes, 10.52 g/kg for TSP, 8.52 g/kg for $PM_{10}$, 6.23 g/kg for $PM_{2.5}$, 72.86 g/kg for CO, 11.73 g/kg for NO, 0.20 g/kg for $SO_2$, 47.10 g/kg for TVOC and 0.20 g/kg for $NH_3$.

The Technical Implementation Scheme and Probeems That Need to be Solved for Realizing the Natinal 2G-3G Roaming (국내 2G-3G간 로밍 구현방안과 해결과제)

  • 정수성
    • Journal of the Korean Professional Engineers Association
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    • v.34 no.5
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    • pp.69-72
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    • 2001
  • The national 2G-3G roaming is emerging as one of essential and important issues In Korea in connection with the recent granting of 3G licenses. Accustomed to the current seamless coverage and superb quality of 2G networks, many subscribers have very high expectations of an equal or better service provisioning by 3G networks. However, it is very unlikely for 3G operators to successfully build their networks as comprehensive as the current 2G networks in the beginning of network implementation and commercial launch. Therefore,3G operators are seriously considering the national 2G-3G roaming in Korea. In this paper. 1 would like to discuss and present a technical implementation scheme and problems that need to be solved fer successfully realizing the national 2G-3G roaming.

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