Effect of Medium Compositions on the Mycelial Growth of Inonotus obliquus

Effect of temperature($22-32^{\circ}C$), pH(5-7) and medium composition on the mycelial growth for the submerged culture of Inonotus obliquus. The concentrations of glucose, starch, peptone, yeast extract, $K_2HPO_4$, $MgSO_4{\cdot}7H_2O$ and $CaCl_2$ were examined in the ranges of 30-120 g/L, 0-10 g/L, 0-20 g/L, 0-15 g/L, 0-2 g/L, 0-1.5 g/L and 0-0.5g/L, respectively. The maximum mycelial growth of Inonotus obliquus was obtained for $26-27^{\circ}C$ and pH 6. The concentrations of glucose, yeast extract and $CaCl_2$, which gave the maximum mycelial growth of Inonotus obliquus, were 70 g/L, 5 g/L and 0.1 g/L, respectively. In the cases of starch, peptone and $K_2HPO_4$, the mycelial growth of Inonotus obliquus increased with increasing the concentrations. However, as the concentration of $MgSO_4{\cdot}7H_2O$ increased, the mycelial growth of Inonotus obliquus decreased. The medium for maximum mycelial growth of Inonotus obliquus consisted of (per 1 L): glucose, 70 g; peptone, 5-20 g; starch, 10 g; yeast extract, 5 g; $K_2HPO_4$, 2 g and $CaCl_2$, 0.1 g.

차가버섯의 균사체 성장에 대한 배지성분의 영향

차가버섯(Inonotus obliquus) 균사체의 액체배양에서 온도($22-32^{\circ}C$)와 pH(5-7) 그리고 배지성분의 변화가 차가버섯 균사체의 성장에 미치는 연구를 수행하였다. Glucose, starch, peptone, yeast extract, $K_2HPO_4$, $MgSO_4{\cdot}7H_2O$ 그리고 $CaCl_2$를 각각 30-120 g/L, 0-10 g/L, 0-20 g/L, 0-15 g/L, 0-2 g/L, 0-1.5 g/L, 0-0.5 g/L의 범위로 변화시키면서 실험하였다. 차가버섯 균사체의 성장속도는 $26-27^{\circ}C$, pH 6, glucose 70 g/L, yeast extract 5 g/L 그리고 $CaCl_2$ 0.1 g/L일 때에 최대값을 나타났다. Starch와 peptone 그리고 $K_2HPO_4$의 농도가 증가할수록 차가버섯 균사체의 성장속도는 증가하였으나, $MgSO_4{\cdot}7H_2O$의 농도가 증가할수록 차가버섯 균사체의 성장속도는 감소하였다. 차가버섯 균사체의 최대 성장 속도를 위한 액체배지는 glucose 70 g/L, peptone 5-20 g/L, starch 10 g/L, yeast extract 5 g/L, $K_2HPO_4$ 2 g/L 그리고 $CaCl_2$ 0.1 g/L로 구성된다.