• Animal Systematics, Evolution and Diversity
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• 제40권1호
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• pp.79-87
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• 2024

A taxonomic examination of hydroids collected from the subtidal zones of Ulleungdo and Dokdo located in the East Sea of Korea from 2017 to 2023 was conducted. Through this study, Antennella aurantia n. sp., a new species belonging to the family Halopterididae, was newly added to the hydrozoan fauna of Korea. This new species is clearly distinguished from similar species by the different morphology and size of the hydrothecae, lateral nematothecae, and gonothecae. By comparing the 16S rRNA sequence with 7 recorded species within the Antennella, the interspecific genetic distance to the new species was 8.2-28.9%, which is very high compared to the intraspecific variation of 0.0%, showing that it was clearly distinguished from other species. As a result of this study, a total of 4 Antennella species are reported in the hydrozoan fauna of Korea.

• Journal of Microbiology and Biotechnology
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• 제29권4호
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• pp.527-537
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• 2019

Three types of meongge (Halocynthia roretzi) jeotgal (MJ) were prepared with 3 different types of salts (12%, w/v): purified salt (PS), solar salt aged for 3 years (SS), and bamboo salt that had been recrystalized 3 times (BS). One set of MJ was fermented with starters, Bacillus subtilis JS2 and Tetragenococcus halophilus BS1-37 (each 6 log CFU/g), and another set without starters for 42 days at $10^{\circ}C$. The LAB count of the SSMJ (non-starter) was highest at day 28 (2.30 log CFU/g). The pH of the PSMJ and SSMJ was 5.72-5.77 at day 0, and 5.40-5.50 at day 42. BSMJ showed higher pH and lower titratable acidities than other samples. Amino-type nitrogen (ANN) increased continuously, and SSMJ showed higher values than other samples from day 14. Bacterial species of non-starter MJ were examined by culture independent method. Clone libraries of 16S rRNA genes were constructed in Escherichia coli from total DNA from non-starter MJ samples at day 0, 14, and 28. Thirty clones per each sample were randomly selected and DNA sequences were analyzed. Variovorax sp., uncultured bacterium, and Acidovorax sp. were the most dominant group at day 0, 14, and 28, respectively. Lactobacillus sakei and Streptococcus sp. were the next dominant group in SSMJ at day 28. A Streptococcus sp. was detected from PSMJ at day 28. Sensory evaluation for MJ samples at day 28 showed that SSMJ got higher overall acceptability scores. These results showed that solar salt can cause desirable changes in the microbial community of fermented foods, thereby positively affecting their overall quality.

• Asian-Australasian Journal of Animal Sciences
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• 제25권5호
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• pp.701-707
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• 2012

Immune stress is the loss of immune homeostasis caused by external forces. The purpose of this experiment was to investigate the effects of immune stress on the growth performance, small intestinal enzymes and peristalsis rate, and mRNA expression of nutrient transporters in broiler chickens. Four hundred and thirty-two 1-d-old broilers (Cobb500) were randomly assigned to four groups for treatment; each group included nine cages with 12 birds per cage. Group 1 = no vaccine (NV); Group 2 = conventional vaccine (CV); group 3 = lipopolysaccharide (LPS)+conventional vaccine (LPS); group 4 = cyclophosphamide (CYP)+conventional vaccine (CYP). The results demonstrated that immune stress by LPS and CYP reduced body weight gain (BWG), feed intake (FI), small intestine peristalsis rate and sIgA content in small intestinal digesta (p<0.05). However, the feed conversion ratio (FCR) remained unchanged during the feeding period. LPS and CYP increased intestinal enzyme activity, relative expression of SGLT-1, CaBP-D28k and L-FABP mRNAs (p<0.05). LPS and CYP injection had a negative effect on the growth performance of healthy broiler chickens. The present study demonstrated that NV and CV could improve growth performance while enzyme activity in small intestine and relative expression of nutrient transporter mRNA of NV and CV were decreased in the conditions of a controlled rational feeding environment. It is generally recommended that broilers only need to be vaccinated for the diseases to which they might be exposed.

• ALGAE
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• 제28권2호
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• pp.185-192
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• 2013

A new cold-adapted Arctic strain of Haematococcus pluvialis from Blomstrandhalv${\o}$ya Island (Svalbard) is described. This strain is predominantly always in non-motile palmelloid stage. Transmission electron microscopy showed the presence of very thick cell wall and abundant lipid vesicles in the palmelloids, including red and green cells. The external morphology of the non-motile palmelloid and motile bi-flagellated cells of our strain is similar to H. pluvialis; however it differs from H. pluvialis in physiology. Our strain is adapted to live and produce astaxanthin in the low temperature ($4-10^{\circ}C$), whilst the usual growth temperature for H. pluvialis is between $20-27^{\circ}C$. Phylogenetic analysis based on 18S rRNA gene data showed that our strain nested within the Haematococcus group, forming a sister relationship to H. lacustris and H. pluvialis, which are considered synonymous. Therefore, we identified our Arctic strain as H. pluvialis.

• Genomics & Informatics
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• 제21권3호
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• pp.34.1-34.10
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• 2023

Nosocomial infections, commonly referred to as healthcare-associated infections, are illnesses that patients get while hospitalized and are typically either not yet manifest or may develop. One of the most prevalent nosocomial diseases in hospitalized patients is pneumonia, among the leading causes of mortality and morbidity. Viral, bacterial, and fungal pathogens cause pneumonia. More severe introductions commonly included Staphylococcus aureus, which is at the top of bacterial infections, per World Health Organization reports. The staphylococci, S. aureus, strain RMI-014804, mesophile, on-sporulating, and non-motile bacterium, was isolated from the sputum of a pulmonary patient in Pakistan. Many characteristics of S. aureus strain RMI-014804 have been revealed in this paper, with complete genome sequence and annotation. Our findings indicate that the genome is a single circular 2.82 Mbp long genome with 1,962 protein-coding genes, 15 rRNA, 49 tRNA, 62 pseudogenes, and a GC content of 28.76%. As a result of this genome sequencing analysis, researchers will fully understand the genetic and molecular basis of the virulence of the S. aureus bacteria, which could help prevent the spread of nosocomial infections like pneumonia. Genome analysis of this strain was necessary to identify the specific genes and molecular mechanisms that contribute to its pathogenicity, antibiotic resistance, and genetic diversity, allowing for a more in-depth investigation of its pathogenesis to develop new treatments and preventive measures against infections caused by this bacterium.

• 미생물학회지
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• 제49권4호
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• pp.369-376
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• 2013

인삼 근계(근권, 근면, 근내부)로부터 ginsenoside Rb1 전환효소인 ${\beta}$-glucosidase 생산 균주(BGB)를 분리하였다. 인삼 근계부터 분리된 BGB 28균주의 계통학적 특성을 확인한 결과, 근권에서 Stenotrophomonas 속(3균주), Pseudoxanthomonas 속(1균주), Bacillus 속(1균주)로 확인되었다. 근면로부터 분리된 BGB는 Stenotrophomonas 속(16균주), Streptomyces 속(1균주), Microbacterium 속(1균주)이며, 근내부는 Stenotrophomonas 속(3균주), Lysobacter 속(2균주)를 포함하는 다양한 계통군이 확인 되었다. 특히 인삼 근계로부터 분리된 BGB 균주의 90%가 Stenotrophomonas 계통군에 속하는 특징을 나타내었다. 근권으로부터 분리된 4KR4 균주는 108.17 unit의 ${\beta}$-glucosidase 활성을 나타내었으며, ginsenoside Rb1을 Rd, Rg3 그리고 minor ginsenoside Rh2로 전환되었다. 4KR4 균주는 Stenotrophomonas rhizophila e-$p10^T$ (AJ293463)와 99.65%의 높은 상동성을 나타내었다. 본 연구에서 분리된 ginsenoside 전환세균 4KR4 균주의 계통학적 위치와 표현형적 특징, 균체 지방산조성, 생리 생화학적 특성을 검토한 결과, Stenotrophomonas sp. 4KR4 (=KACC 17635) 균주로 확인되었다.

• 미생물학회지
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• 제49권1호
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• pp.38-45
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• 2013

본 연구에서는 연안갯벌 갯지렁이(Perinereis aibuhitensis)에서 분리한 광염성 미생물, Bacillus sp. EBW4의 특성과 다양한 환경조건에서 유기물 분해능을 분석하였다. 16S rRNA 염기서열에 기초하여 동정한 결과, 균주 EBW4는 Bacillus hemicentroti $JSM076093^T$와 98.3%, Bacillus hwajinponensis SW-$72^T$와 97.96% 그리고 Bacillus algicoa $KMM3737^T$와 96.28%의 상동성을 보였다. EBW4의 생장 가능한 온도 범위는 $4-40^{\circ}C$, 염분도 범위는 0-17%, pH 범위는 5-9로 나타나 EBW4는 광염성 균주로 밝혀졌다. EBW4의 세포막을 구성하는 주요 지방산으로는 anteiso $C_{15:0}$, iso $C_{16:0}$, anteiso $C_{17:0}$, iso $C_{14:0}$ 등으로 각각 48.2, 12.1, 11.6, 9.4% 비율로 나타났다. EBW4는 탄수화물, 단백질, 지방 등 다양한 고분자 유기물을 분해할 수 있는 DNase, amylase, protease, lipase 등의 효소 활성뿐만 아니라, alkaline phosphatase, esterase (C4), leucine arylamidase 그리고 ${\alpha}$-chymotrypsin 효소활성도 가지고 있었다. 다양한 염분 농도 조건에서 합성폐수를 이용한 실험에서 EBW4은 조사한 모든 범위의 염분 조건에서도 유기물 분해능이 우수하였다.

• 동의생리병리학회지
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• 제16권4호
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• pp.801-809
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• 2002

CSBHT is known to improve immunological response in mice and humans. In this study, CSBHT effect was examined in the context of CD4+ T cells' survival and TCR/CD3 induced activation responses. Spleen cells from 8 week BALB/c mice were cultured in CSBHT containing medium without activation for 24, 48 hr. The MTS assay and revealed that CSBHT did not stimulate spleen lymphocytes as mitogen. Spleen lymphocytes were treated with anti-CD3e/anti-CD28 antibodies for 48hr. Flow cytometry revealed that activity of T cell decreased with CSBHT concentration. CD4+ T cells were isolated and cultured ,in CSBHT containing medium for 48 hr. CSBHT did not affect survival of sorted CD4+ T cells without any involvement of APC. In order to evaluate the direct effect of CSBHT on helper T cells's proliferative capacity prior to activation, CD4+ T cells are isolated after 24hr of culture in CSBHT containing medium and activated with and without anti-CD3e/anti-CD28 activation for 48hr. A higher level of CD69 was observed in 1 ㎍/㎖ of CSBHT treatment than control using flow cytometry. But low CD69 expression was observed in 5㎍/㎖ of CSBHT treatment. Expression of mRNA for cytokines in CD4+ T cell revealed that IL-2 expression was increased in 1 ㎍/㎖. The expression of IL-2R α, INF- γ were increased with concentration. On the other hand mRNA of IL-4 was decreased in dose dependent manner. Results suggest that CSBHT may be desirable for CD4+ T cell's activity in immune responses. Further more, CSBHT may relatively activate Th1 and inactivate Th2.

• 한국유기농업학회지
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• 제28권2호
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• pp.235-250
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• 2020

작물 재배 방법이 토양의 미생물 군집 특성과 화학성에 미치는 영향을 검토하였다. 이를 위해서 다섯 곳의 고추 관행 재배지와 다섯 곳의 유기농 재배지 토양을 채취한 후, 16S rRNA 유전자 기반의 파이로시퀀싱 기법으로 미생물 군집을 조사하였다. 분석 결과 총 22개의 세균 문으로 구성되었으며 Proteobacteria 문(33.0 ± 5.7%), Actinobacteria 문(19.9 ± 9.7%) 및 Firmicutes 문(13.6 ± 5.0%)이 우점하였고, 이들은 전체 상대풍부도의 66%를 차지하였다. 고추 관행 재배지와 유기농 재배지의 미생물 군집 분포를 비교했을 때 전반적으로 서로 다른 군집 특성을 나타내었다. 또한 관행 재배 토양에서는 Actinobacteria 문과 Chloroflexi 문이 상대적으로 풍부하였고, 유기 재배 토양에서는 Proteobacteria 문과 Firimicutes 문이 상대적으로 풍부하였다. 특히 Streptomyces 속과 Bacillus 속의 상대풍부도는 관행 재배 토양과 유기 재배 토양 간에 상당한 차이를 보였다. 또한 토양 화학 성에 의하여 세균 군집 변화가 관찰되었는데, Proteobacteria 문의 Rhizobiales 목과 Actinobacteria 문의 Streptomyces 속은 pH에 의해 세균 군집이 바뀌었고, Firimicutes 문의 Bacillus 속은 유기물 함량에 의해 군집 분포가 바뀌었다. 본 연구결과는 재배관리에 따른 토양의 물리-화학성의 변화가 미생물 군집 분포에 뚜렷하게 관련(p<0.05)되어 있다는 것을 보여주었다.

• 미생물학회지
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• 제51권1호
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• pp.48-60
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• 2015

순창군에서 제조된 전통 장류로부터 612종의 다양한 미생물을 분리하였고, 612종의 미생물 중에서 생리활성 분석 및 biogenic amine 생성여부를 조사하여 최종적으로 Bacillus subtilis SCJ4를 선별하였다. 최종 선별한 균주 SCJ4의 생화학적 특성과 16S rRNA 염기서열 분석에 의해 B. subtilis SCJ4로 명명하였으며, B. subtilis SCJ4의 산업적으로 사용이 적합한 최적배지조성을 확립하기 위하여 통계학적 방법인 반응표면 분석법을 사용하였다. 실험계획법은 배지조성을 예측하기 위해 Plackett-Burman design을 사용하여 모델을 설정하였다. 실험 모델을 통하여 선별된 배지 조성으로는 tryptone, peptone, $MgSO_4$로 예측되었으며, 배지조성에 대한 농도를 설정하기 위해 중심합성계획법을 사용하여 최적화를 수행하였다. 최종 예측된 배지 조성의 농도는 tryptone 15.35 g/L, peptone 12.235 g/L, $MgSO_4$ 3.5 g/L로 예측되었다. 이때 최대 균체량은 1.07497 g/L로 예측되었으며, 모델의 검증실험을 통하여 예측된 모델과 실제 결과가 동일함을 확인하였으며, 기본배양배지와 비교하였을 때 균체량이 0.8767 g/L에서 1.1222 g/L으로 약 28%증가하였다.