[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.7845/kjm.2013.005

Characterization and Organic Hydrocarbons Degradation Potential of Euryhaline Marine Microorganism, Bacillus sp. EBW4 Isolated from Polychaete (Perinereis aibuhitensis)

Shin, Seyeon (Department of Environmental Education, Sunchon National University)
Yundendorj, Khorloo (Department of Environmental Education, Sunchon National University)
Lee, Sang-Suk (Department of Animal Science and Technology, Sunchon National University)
Lee, Dong-Heon (The Research Institute for Basic Sciences, Jeju National University)
Kang, Kyoung-Ho (Department of Aquaculture, Chonnam National University)
Kahng, Hyung-Yeel (Department of Environmental Education, Sunchon National University)

Publication Information

Korean Journal of Microbiology / v.49, no.1, 2013 , pp. 38-45 More about this Journal

Abstract

In this study, euryhaline marine microorganism, Bacillus sp. strain EBW4 isolated from polychaete (Perinereis aibuhitensis) of Suncheon Bay was physiologically, biochemically and genetically characterized. Based on 16S rRNA sequence, EBW14 was found to share 98.25% similarity with Bacillus hemicentroti $JSM076093^T$ , 97.96% similarity with Bacillus hwajinponensis SW- $72^T$ and 96.28% similarity with B. algicoa $KMM3737^T$ , respectively. The temperature range for the growth of strain EBW4 was $4-40^{\circ}C$ , NaCl concentration range 0-17% and pH range pH 5-9, revealing that EBW4 was euryhaline bacterium. Major fatty acids in strain EBW4 were composed of anteiso $C_{15:0}$ (48.2%), iso $C_{16:0}$ (12.1%), anteiso $C_{17:0}$ (11.6%) and iso $C_{14:0}$ (9.4%). EBW4 was found to have DNase, amylase, protease and lipase for the degradation of macromolecules such as DNA, carbohydrates, proteins, lipids, etc. The enzyme activities of alkaline phosphatase, esterase (C4), leucine arylamidase and ${\alpha}$ -chymotrypsin were also found in strain EBW4. Analysis of the biodegradation ability of EBW4 for organic hydrocarbons under different salinity conditions using synthetic water waste revealed that EBW4 exhibited the ability to degrade organic hydrocarbons very quickly, suggesting strain EBW4 may be a good candidate for the application to various industries.

Keywords

Bacillus; Perinereis aibuhitensis; euryhaline microorganism; organic hydrocarbons degradation;

Citations & Related Records

Reference

1	Vaseeharan, B. and Ramasamy, P. 2003. Control of pathogenic Vibrio spp. by Bacillus subtilis BT23, a possible probiotic treatment for black tiger shrimp Penaeus monodon. Lett. Appl. Microbiol. 36, 83-87. DOI ScienceOn
2	Verschuere, L., Rombaut, G., Sorgeloos, P., and Verstraete, W. 2000. Probiotic bacteria as biological control agents in aquaculture. Microbiol. Mol. Biol. Res. 64, 655-671. DOI ScienceOn
3	Vigneswaran, S., Ngo, H.H., and Wee, K.L. 1999. Effluent recycle and waste minimisation in prawn farm effluent. J. Cleaner Prod. 7, 121-126. DOI ScienceOn
4	Yoon, J.H., Kim, I.G., Kang, K.H., Oh, T.K., and Park, Y.H. 2004. Bacillus hwajinponensis sp. nov. and an unnamed Bacillus genomospecies, novel members of Bacillus rRNA group 6 isolated from sea water of the East Sea and the Yellow Sea in Korea. Int. J. Syst. Evol. Microbiol. 54, 803-808. DOI ScienceOn
5	Ziaei-Nejad, S., Rezaeib, M.H., Takamic, G.A., Lovettd, D.L., Mirvaghefia, A., and Shakourie, M. 2006. The effect of Bacillus spp. bacteria used as probiotics on digestive enzyme activity, survival and growth in the Indian white shrimp Fenneropenaeus indicus. Aquacult. 252, 516-524. DOI ScienceOn
6	Kumar, S., Nei, M., Dudley, J., and Tamura, K. 2008. Mega : a biologist-centric software for evolutionary analysis of DNA and protein sequences. Brief Bioinform. 9, 299-306. DOI ScienceOn
7	Lane, D.J. 1991. 16S/23S rRNA sequencing. In Stackebrandt, E. and Goodfellow, M. (eds.), Nucleic acid techniques in bacterial systematics. pp. 115-75. John Wiley and Sons, New York, N.Y., USA.
8	Maniatis, T., Fritsch, E.F., and Sambrook, J. 1982. Molecular cloning. cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y., USA.
9	Mikesell, M.D., Kukor, J.J., and Olsen, R.H. 1993. Metabolic diversity of aromatic hydrocarbon-degrading bacteria from a petroleumcontaminated aquifer. Biodegradation 4, 249-259.
10	Moeller, M. 1891. Ueber eine neue Methode der Sporenfarbung. Zbl. Bakt. I. Abt. Orig. 10, 273-277.
11	Moriarty, D.J.W. 1998. Control of luminous Vibrio species in penaeid aquaculture ponds. Aquatic zooremediation: deploying animals to remediate contaminated aquatic environments. Aquacult. 164, 351-358. DOI ScienceOn
12	Orejas, C., Gili, J.M., Arntz, W.E., Ros, J.D., Lopez, P.J., Teixido, N., and Filipe, P. 2000. Benthic suspension feeders, key players in Antarctic marine ecosystems. Contribut. Sci. 1, 299-311.
13	Perez, E., Blasco, J., and Sole, M. 2004. Biomarker responses to pollution in two invertebrate species: Scrobicularia plana and Nereis diversicolor from the Cadiz bay (SW Spain). Mar. Environ. Res. 58, 275-279. DOI ScienceOn
14	Palacios, G.L. and Timmons, M.B. 2001. Determining design parameters for recovery of aquaculture wastewater using sand beds. Aquacult. Engineer. 24, 289-299. DOI ScienceOn
15	Prieur, D., Mével, G., Nicolas, J.L., Plusquellec, A., and Vigneulle, M. 1990. Interactions between bivalve molluscs and bacteria in the marine environment. Oceanogr. Mar. Biol. Ann. Rev. 28, 277-352.
16	Raida, M.K., Larsen, J.L., Nielsen, M.E., and Buchmann, K. 2003. Enhanced resistance of rainbow trout, Oncorhynchus mykiss (Walbaum), against Yersinia ruckeri challenge following oral administration of Bacillus subtilis and B. licheniformis (BioPlus2B). J. Fish. Dis. 26, 495-498. DOI ScienceOn
17	Saitou, N. and Nei, M. 1987. The neighbor-joining method : a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406-425.
18	Stabili, L., Licciano, M., Giangrande, A., Fanelli, G., and Cavallo, R.S. 2006. Sabella spallanzanii filter-feeding on bacterial community: ecological implications and applications. Mar. Environ. Res. 61, 74-92. DOI ScienceOn
19	Taubel, M., Kampfer, P., Buczolits, S., Lubitz, L., and Busse, H.-J. 2003. Bacillus barbaricus sp. nov., isolated from an experimental wall painting. Int. J. Syst. Evol. Microbiol. 53, 725-730. DOI ScienceOn
20	Sugita, H., Hirose, Y., Matsuo, N., and Deguchi, Y. 1998. Production of the antibacterial substance by Bacillus species strain NM12, an intestinal bacterium of Japanese coastal fish. Aquacult. 165, 269-280. DOI ScienceOn
21	Fujioka, Y., Shimoda, T., and Srithong, C. 2007. Diversity and community structure of macrobenthic fauna in shrimp aquaculture ponds of the Gulf of Thailand. Jap. Agric. Res. Quart. 41, 163-172. DOI
22	Gatesoupe, F.J. 1999. The use of probiotics in aquaculture. Aquacult. 180, 147-165. DOI ScienceOn
23	Ghosh, K., Sen, S.K., and Ray, A.K. 2002. Characterization of bacilli isolated from the gut of rohu, Labeo rohita fingerlings and its significance in digestion. J. Appl. Aquacult. 12, 33-42. DOI
24	Gili, J.M. and Coma, R. 1998. Benthic suspension feeders : their paramount role in littoral marine food webs. Trends Ecol. Evol. 13, 316-321. DOI ScienceOn
25	Gomez-Gil, B., Roque, A., and Turnbull, J.F. 2000. The use and selection of probiotic bacteria for use in the culture of larval aquatic organisms. Aquacult. 191, 259-270. DOI ScienceOn
26	Guo, H., Luo, S., Chen, L., Xiao, X., Xi, Q., Wei, W., Zeng, G., Liu, C., Wan, Y., and Chen, J. 2010. Bioremediation of heavy metals by growing hyperaccumulaor endophytic bacterium Bacillus sp. L14, Bioresour. Technol. 101, 8599-8605. DOI ScienceOn
27	Guncheva, M. and Zhiryakova, D. 2011. Catalytic properties and potential applications of Bacillus lipases. J. Mol. Cat. B Enzym. 68, 1-21. DOI ScienceOn
28	Hall, T. 2007. BioEdit. Biological sequence alignment editor for Win95/98/NT/2K/XP. Ibis Biosciences, Carlsbad, CA, USA.
29	Hucker, G.J. and Cohn, H.J. 1923. Methods of Gram staining. Tech. Bull. N.Y. State Agric. Exp. Sta. 93.
30	Hovda, M.B., Sivertsvik, M., Lunestad, B.T., Lorentzen, G., and Rosnes, J.T. 2007. Characterisation of the dominant bacterial population in modified atmosphere packaged farmed halibut (Hippoglossus hippoglossus) based on 16S rDNA-DGGE. Food Microbiol. 24, 362-371. DOI ScienceOn
31	Hugh, R. and Leifson, E. 1953. The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various Gram-negative bacteria. J. Bacteriol. 66, 24-26.
32	Ivanova, E.P., Alexeeva1, Y.A., Zhukova, N.V., Gorshkova, N.M., Buljan, V., Nicolau, D.V., Mikhailov, V.V., and Christen, R. 2004. Bacillus algicola sp. nov., a novel filamentous organism isolated from brown alga Fucus evanescens. System. Appl. Microbiol. 27, 301-307. DOI ScienceOn
33	Jeffries, C.D., Holtman, D.F., and Guse, D.G. 1957. Rapid method for determining the activity of microorganisms on nucleic acids. J. Bacteriol. 75, 590-591.
34	Joo, H.-S., Kumar, C.G., Park, G.-C., Paik S.R., and Chang, C.-S. 2004. Bleach-resistant alkaline protease produced by Bacillus sp. isolated from the Korean polychaete, Periserrula leucophryna. Process Biochem. 39, 1441-1447. DOI ScienceOn
35	Kapdan, I.K. and Erten, B. 2007. Anaerobic treatment of saline wastewater by Halanaerobium lacusrosei. Process Biochem. 42, 449-453. DOI ScienceOn
36	Kennedy, S.B., Tucker, J.W., Neidig, C.L., Vermeer, G.K., Cooper, V.R., Jarrell, J.L., and Sennett, D.G. 1998. Bacterial management strategies for stock enhancement of warm water marine fish : a case study with common snook (Centropomus undecimalis). Bull. Mar. Sci. 62, 573-588.
37	Arena, A., Maugeri, T.L., Pavone, B., Iannello, D., Gugliandolo, C., and Bisignano, G. 2006. Antiviral and immunoregulatory effect of a novel exopolysaccharide from a marine thermotolerant Bacillus licheniformis. Int. Immunopharmacol. 6, 8-13. DOI ScienceOn
38	Bishop, P.J. and Neuman, G. 1970. The history of the Ziehl-Neelsen stain. Tubercle. 51, 196. DOI ScienceOn
39	Bagheri, T., Hedayati, S.A., Yavari, V., Alizade, M., and Farzanfar, A. 2008. Growth, survival and gut microbial load of rainbow trout (Onchorhynchus mykiss) fry given diet supplemented with probiotic during the two months of first feeding. Turk. J. Fish. Aquat. Sci. 8, 43-48.
40	Banerjee, S., Devaraja, T.N., Shariff, M., and Yusoff, F.M. 2007. Comparison of four antibiotics with indigenous marine Bacillus spp. in controlling pathogenic bacteria from shrimp and Artemia. J. Fish Dis. 30, 383-389. DOI ScienceOn
41	Bryan, G.W. and Gibbs, P.E. 1987. Polychaete common ragworms as indicators of heavy-metal availability in marine deposits, pp. 37-39. In Capuzzo, J.M. and Kester, D.R. (eds.), Biological Processes and Wastes in the Oceans. Robert E Krieger Publishing Co., Malabar, Florida, USA.
42	Campos, L.C., Su, M.F.J., Graham, N.J.D., and Smith, S.R. 2002. Biomass development in slow sand filters. Water Res. 36, 4543-4551. DOI ScienceOn
43	Chen, Y.G., Zhang, Y.Q., He, J.W., Klenk, H.P., Xiao, J.Q., Zhu, H.Y., Tang, S.K., and Li, W.J. 2011. Bacillus hemicentroti sp. nov., a moderate halophile isolated from a sea urchin. Int. J. Syst. Evol. Microbiol. 61, 2950-2955. DOI ScienceOn
44	Chun, J., Lee, J.H., Jung, Y., Kim, M., Kim, S., Kim, B.K., and Lim, Y.W. 2007. EzTaxon : a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int. J. Syst. Evolution Microbiol. 57, 2259-2261. DOI ScienceOn
45	Davidson, J., Helwig, N., and Summerfelt, S.T. 2008. Fluidized sand biofilters used to remove ammonia, biochemical oxygen demand, total coliform bacteria, and suspended solids from an intensive aquaculture effluent. Aquacult. Engineer. 39, 6-15. DOI ScienceOn
46	Felsenstein, J. 1985. Confidence limits on phylogenies : an approach using the bootstrap. Evolution 39, 783-791. DOI ScienceOn
47	Fourcy, D., Jumel, A., Heydorff, M., and Lagadic, L. 2002. Esterases as biomarkers in Nereis (Hediste) diversicolor exposed to temephos and Bacillus thuringiensis var. israelensis used for mosquito control in coastal wetlands of Morbihan (Brittany, France). Mar. Environ. Res. 54, 755-759. DOI ScienceOn