• Asian Pacific Journal of Cancer Prevention
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• v.16 no.8
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• pp.3195-3201
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• 2015

Objectives: To investigate the effects of betaine on HeLa cell growth and apoptosis and molecular mechanisms. Materials and Methods: Concentrations of 0.1, 1.0, 5.0, 20.0, 100.0 mg/ml of betaine were used to evaluate the anticancer efficacy for HeLa cells respectively, and MCF-10A was also detected as a normal diploid cell control. Results: We found that proliferation of HeLa cells was inhibited significantly upon exposure to increasing betaine levels with the MTT test (p<0.05). The percentage of S phase cells in the low dose groups (<5mg/ml) were distinctly higher than in high dose groups, and the rates of Sub-G1 phase were the opposite (p<0.01); A high concentration of betaine (>5.0mg/ml) significantly promoted the apoptosis of HeLa cells (p<0.01). SOD activities of the low dose groups were slightly higher than the control group (p<0.05) and there were obvious synchronicity and correlation among the expression of promoting apoptosis genes Bax, P53, Caspase 3 and apoptosis suppression gene Bcl-2. In response to an apoptosis-inducing stimulus, p53 and cyclin D1 could be activated with blockage of the cell cycle at G1/S or S/G2 checkpoints. Conclusions: Our data showed that betaine could promote HeLa cells proliferation in vitro at low concentrations. In contrast, high concentrations could significantly inhibit cell growth and migration, and induce apoptosis of HeLa cells through caspase 3 signaling and further promoted necrosis. This might imply that betaine exhibits tumoricidal effects and acts as a biological response modifier in cancer treatment by inducing apoptosis and cell cycle arrest in a dose and time-dependent manner.

• Korean Journal of Environmental Biology
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• v.24 no.1 s.61
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• pp.46-52
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• 2006

The mercury is among the most highly bioconcentrated toxic trace metals. Many national and international agencies and organisations have targeted mercury for the possible emission control. The mercury toxicity depends on its chemical form, among which alkylmercury compounds are the most toxic. A human cervix uterus cancer cell line HeLa cells was employed to investigate the effect of the toxic heavy metal mercury (Hg) and ionizing radiation. In the in vitro comet assays for the genotoxicity in the HeLa cells, the group of Hg treatment after irradiation showed higher DNA breakage than the other groups. The tail extent moment and olive tail moment of the control group were $4.88{\pm}1.00\;and\;3.50{\pm}0.52$ while the values of the only Hg treatment group were $26.90{\pm}2.67\;and\;13.16{\pm}1.82$, respectively. The tail extent moment and olive tail moment of the only 0.001, 0.005, 0.01 Hg group were $12.24{\pm}1.82,\;8.20{\pm}2.15,\;20.30{\pm}1.30,\;12.26{\pm}0.52,\;40.65{\pm}2.94\;and \;20.38{\pm}1.49$, respectively. In the case of Hg treatment after irradiation, the tail extent moment and olive tail moment of the 0.001, 0.005, 0.01 Hg group were $56.50{\pm}3.93,\;32.69{\pm}2.48,\;62.03{\pm}5.14,\;31.56{\pm}1.97,\;72.73{\pm}3.70\;and \;39.44{\pm}3.23$, respectively. The results showed that Hg induced DNA single-strand breaks or alkali labile sites as assessed by the Comet assay. It is in good agreement with the reported results. The mercury inhibits the repair of DNA. The bacterial formamidopyrimidine-DNA glycosylase (Epg protein) recognizes and removes some oxidative DNA base modifications. Enzyme inactivation by Hg (II) may therefore be due either to interactions with rysteine residues outside the metal binding domain or to very high-affinity binding of Hg (II) which readily removes Zn (II) from the zinc finger.

• Proceedings of the KIEE Conference
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• 1987.11a
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• pp.412-414
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• 1987

Theoretical studies of concentration for negative ion and neutral species in electrical excitation discharge have been conducted at $CO_2-N_2-He=1-7-12$, total pressure 20torr. With a computer model of neutral and negative ion processes we have calculated that the concentration of co was less than 20% when expected molecular.

• Communications of the Korean Mathematical Society
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• v.20 no.2
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• pp.365-379
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• 2005

The purpose of this paper is to correct erroneous proofs of theorems of He, Lu, Wang and Zheng [1] about the topological pressure of continuous flows without fixed points and to give proofs of some results which are stated in the literature without proofs and thereby to show that their theorems are still true.

• Bulletin of the Korean Chemical Society
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• v.20 no.6
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• pp.705-711
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• 1999

The laser-induced transient grating method is applied to study the dynamics of the nematic MBBA film. The nanosecond laser pulses of 355 nm are used to make the transient grating and the cw He-Ne laser of 633 nm is used to probe the dynamics. Strong multiple order diffractions are observed at high nematic temperatures. The reordering process induced by the phototransformed state, which is the locally melted state from the nematic sample, is attributed to the main origin of the multiple order diffractions from the nematic MBBA. The characteristics of the multiple order gratings are discussed with the grating profiles simulated from the multiple diffraction signals.

• The Journal of Korean Philosophical History
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• no.57
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• pp.31-55
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• 2018

Professor Dowon (道原) Lew, Seung-Kook (柳承國: 1923~2011) was a master of Eastern philosophy in Korea, in the 20th century. 'Confucianism' was the root of his discipline. Nevertheless he studied extensively throughout Eastern philosophy. He was also a pioneer of 'Korean Philosophy', who devoted himself to 'establishing the original form (原型) of Korean thought'. Professor Lew showed a unique view on how Confucian thought was formed. He has done many years of precise comparative analysis of the results of archaeological studies before 1970, particularly the study of bone-and-shell inions, and what was said in previous literature. As a result, he concluded that "we cannot discuss the origins of Confucian thought by separating the relationship with the Dongyi tribe". Confucianism was formed in relation to Dongyi. The purpose of his attention to the Dongyi tribe - RenFang tribe (人方族) was not to examine Dongyi tribe and its culture. His purpose was to examine how Confucian thought was formed and to examine the relevance of Dongyi tribe in this process. This was in conjunction with the task of exploring 'the original form of Korean thought', whether he pretended or not. Professor Lew, Seung-Kook's theory differed not only from the conventional view of the academic world in his time but also from the conventional view up to now. It is a pity that it was not yet discussed it in the academic world. I consider that it is necessary to seriously review Professor Lew and Seung-Kook's theory at this point when China has outlined the Northeast project (東北工程).

• Journal of Korean Society of Industrial and Systems Engineering
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• v.12 no.20
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• pp.91-95
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• 1989

The activities of Quality assurance, in the past, were attached too much importance to the functions and performanced of products. Because of these practices, in general. product safeties were usually minimized corenen more disregarded from the central points. Qualify assurance should he accepted as one of a major management policies to satisfied with the users needs.

• Journal of Plant Biology
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• v.4 no.1
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• pp.16-20
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• 1961

The author described four triple hybrids by investigating 5000 specimens collected during a period of ten years from all parts of Korea. He concluded that Key factors used for an identification of triple hybrids were mainly trichom types and acorn cup scales. With a plate showing hybrid plants and fruiting characters (latin and Korean).

• (The)Study of the Eastern Classic
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• no.35
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• pp.415-445
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• 2009

A well-known historian, Ahn Jeong-Bok(Sun-Am, 1712~1791) was an expert on the country village community. He was a scholar of the "Nam-In" group, who was excluded from the corridors of power in those days. He kept on the move in various parts of country villages from his childhood. After settling down in Deok-Gok, Kwang-Ju, he stayed in the place devoting himself to the self-culture and the literary works. By his surrounding of environment, he had an academic interest in a concrete science rather than metaphysics and country villages rather than the central city. He considered the country villages as the link holding between a family and a country and had the conception of a confucianist community based on country villages, emphasizing the practice of confucianist virtues in everyday life. First of all, his confucianist community was the community based on country villages. He thought that the enlightenment was a matter of great importance for solving problems in country villages. As a solution to those problems, he suggested Hyang-Yak, the self-governed regulations of country villages. In his own village he made the self-governed rules Dong-Yak. When he was a provincial governer of Mok-Cheon, he put Hyang-Yak, the self-governed regulations of country villages in operation. It aimed for a kind of gentry-centric country village community. But Hyang-Yak was the regulations based on the agreement with each other, stressed the regard on the popular mind and the setting the pace of the gentry, and aimed for the harmony and order in a community through the practice of moral virtues in daily life. On the other hand, he had a conception of a country village's academic community. He thought of the development of educational intuitions as a pressing need of the enlightenment of country village. With young people he read confucianist books with comments in a village school, Seo-Jae. In his seventies, he made and put the self-regulations for academic community, Hak-Yak, in operations. It is considered that Hak-Yak was an example of his idea of academic community and his point of view on learning, which emphasized on the coincidence with reading and practice.

• Korean Institute of Interior Design Journal
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• v.20 no.6
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• pp.300-310
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• 2011

Recently, a new paradigm for the change in recognition on educational spaces and their recycling has been spotlighting a concept of community school. However in Korea, there have been few changes in physical environment since the modern education started, due to the inconsistent educational policies and bungled administration. In contrast to Korea, Netherlands has been activating educational spaces to communicate with the local community, reacting to the change in several educational policies for the last 20 years. We, therefore, need to analyze the recent community school of Herman Hertzberger who starting with the Montessori School, came to the fore with a proposal of new relational educational spaces. For linking his relational architectural philosophy to the local community and community school, he applied it to a contact area. He then organized socially sustainable spaces to promote different acts to take place, laying stress on mutual forms and behaviors between spaces and spaces, spaces and people and people and people. In addition to a structural relation with the macroscopic community of architecture, he also created opened spaces, not those where are independent or disconnected, by encouraging users to meet and act in detailed components such as stairs, walls, windows and others. This study aims to consider how Hertzberger's various community spaces in the community school have been created after the 2000s and suggest design plans for educational spaces to communicate, according to the recent educational trend aspiring towards open education.