• Title/Summary/Keyword: 2-Loop Structure

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Molecular Analysis of Promoter and Intergenic Region Attenuator of the Vibrio vulnificus prx1ahpF Operon

  • Lee, Hyun Sung;Lim, Jong Gyu;Han, Kook;Lee, Younghoon;Choi, Sang Ho
    • Journal of Microbiology and Biotechnology
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    • v.25 no.8
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    • pp.1380-1389
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    • 2015
  • Prx1, an AhpF-dependent 2-Cys peroxiredoxin (Prx), was previously identified in Vibrio vulnificus, a facultative aerobic pathogen. In the present study, transcription of the V. vulnificus prx1ahpF genes, which are adjacently located on the chromosome, was evaluated by analyzing the promoter and intergenic region of the two genes. Northern blot analyses revealed that transcription of prx1ahpF results in two transcripts, the prx1 and prx1ahpF transcripts. Primer extension analysis and a point mutational analysis of the promoter region showed that the two transcripts are generated from a single promoter. In addition, the 3' end of the prx1 transcript at the prx1ahpF intergenic region was determined by a 3'RACE assay. These results suggested that the prx1ahpF genes are transcribed as an operon, and the prx1 transcript was produced by transcriptional termination in the intergenic region. RNA secondary structure prediction of the prx1ahpF intergenic region singled out a stem-loop structure without poly(U) tract, and a deletion analysis of the intergenic region showed that the atypical stem-loop structure acts as the transcriptional attenuator to result in the prx1 and prx1ahpF transcripts. The combined results demonstrate that the differential expression of prx1 and ahpF is accomplished by the cis-acting transcriptional attenuator located between the two genes and thereby leads to the production of a high level of Prx1 and a low level of AhpF.

A New Robust Digital Sliding Mode Control with Disturbance Observer for Uncertain Discrete Time Systems

  • Lee, Jung-Hoon
    • Journal of IKEEE
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    • v.15 no.2
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    • pp.149-156
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    • 2011
  • In this paper, a new discrete variable structure controller based on a new sliding surface and discrete version of the disturbance observer is suggested for the control of uncertain linear systems. The reaching phase is completely removed by introducing a new proposed sliding surface. The discrete version of the disturbance observer is derived for the effective compensation of the effect of uncertainties and disturbances. A corresponding control input with the disturbance compensation is selected to guarantee the quasi sliding mode on the predetermined sliding surface for guaranteeing the designed output in the sliding surface from any initial condition to the origin for all the parameter variations and disturbances. By using Lyapunov function, the closed loop stability and the existence condition of the quasi sliding mode is proved. Finally, an illustrative example is presented to show the effectiveness of the algorithm.

Asymmetrical Contouring Control of Biaxial System (2축 시스템의 비대칭 윤곽제어)

  • Sim, Young Bok;Jung, Yu Chul;Lee, Gun Bok
    • Journal of the Korean Society for Precision Engineering
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    • v.14 no.8
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    • pp.65-72
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    • 1997
  • An asymmetrical cross-coupled compensator to improve the contouring performance is proposed. This is a refinement of the structure suggested by Koren. The position loop is closed with a proportional controller as in the uncoupled system. An additional input term proportional to the component of the contour error along the corresponding axis is included. The controller gains are chosen to give an appropriate frequency response and an optimum range for the damping ratio. The effectiveness of the proposed controller is studied by means of digital simulations of the dynamics of the drives and the controller for 4 types of command trajectories: straight line contour, cornering contour, circular contour, elliptic contour. Substantial improvement in contouring performance is obtained for a range of contouring conditions.

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Fabrication and Characteristics of Integrated Nb DC SQUID (집적화된 Nb DC SQUID 소자의 제작 및 특성)

  • Lee, Yong-Ho;Gwon, Hyeok-Chan;Kim, Jin-Mok;Park, Jong-Cheol
    • Journal of the Korean Magnetics Society
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    • v.2 no.3
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    • pp.277-281
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    • 1992
  • We have designed, fabricated and tested an integrated planar DC SQUID which incorporates input coil and mofulation coil in thin film structure. The SQUID uses Nb /Al-oxide /Nb Josephson junctions and Pd shunt resistors, and the SQUID loop incorporates two rings connected in series forming figure '8' structure and has the advantage of a negligibly small circulating current for the spatially homogeneous noise fields. The devices were fabricated using photolithographic technique, RF magnetron sputtering, anodic oxidation for insulation and lift-off process. The preliminary test of the fabricated SQUID at 4.2 K showed that the flux-voltage characteristics were smooth enough to adopt standard readout system, and the voltage noise was too small to be measured by direct method and so the white noise was thought to be less than $10^{-4}\;{\phi}_o/\;\sqrt{H_z}$.

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A Study on the Conversion Efficiency of Rectenna using Dual-Polarization and FSS Method (이중편파와 FSS를 적용한 정류안테나의 변환효율 분석에 관한 연구)

  • 윤동기;박양하;김관호;이영철
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.10 no.5
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    • pp.747-756
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    • 1999
  • In this paper, we analyzed microwave-DC conversion efficiency for the dual-polarization rectenna and antenna position changing. And then we analyzed and applied Square-Loop FSS structure for reducing the diode harmonic components as a rectifying circuit. The results of microwave-DC conversion efficiency for the each of designed dual-polarization rectenna has 69.1% with $360\Omega$ (dipole type) and 75.4% with $340\Omega$ (patch type) optimum load resistor. When the each of dual-polarization rectenna has a optimal load resistor, it's conversion efficiency shows of $\pm$20% in dipole type and $\pm$5% in patch type at $0~180^{\circ}$position. When applied Square-Loop FSS structure for Rectenna, Insertion loss was under 1 dB as the passband and over 20 dB as the stopband. The microwave -DC conversion efficiency was represented good properties of $\pm$2% variation.

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Structure and Function of RGD Peptides Derived from Disintegrin Proteins

  • Kim, Jiun;Hong, Sung-Yu;Park, Hye-seo;Kim, Doo-Sik;Lee, Weontae
    • Molecules and Cells
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    • v.19 no.2
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    • pp.205-211
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    • 2005
  • The Arg-Gly-Asp (RGD) sequence serves as the primary recognition site in extracellular matrix proteins, and peptides containing this sequence can mimic the biological activities of matrix proteins. We have initiated structure-function studies of two RGD containing peptides, RGD-5(AGGDD) and cyclic RGD-6(CARGDDC). Assays have shown that cyclic RGD-peptides inhibit platelet aggregation more efficiently than linear ones. NMR data revealed that RGD-5 and RGD-6 have entirely different conformation. RGD-5 has a linear extended structure and RGD-6 has a stable loop conformation. In RGD-5 the guanidinium group of Arg2 and the carboxyl group of Asp4 lie in parallel, whereas the side-chains of Arg3 and Asp5 of RGD-6 are located in different planes, supporting the idea that the stability of the cyclic form derives from the packing of the side chain of the Arg and Asp residues. The structural features of these peptides could provide a basis for designing new drugs against diseases related to platelet aggregation and as cancer antagonists.

Implementation of Topological Operators for the Effective Non-manifold CAD System (효율적인 복합다양체 CAD 시스템 위상 작업자 구현)

  • 최국헌
    • Proceedings of the Korean Society of Machine Tool Engineers Conference
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    • 2004.10a
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    • pp.382-387
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    • 2004
  • As the increasing needs in the industrial filed, many studies for the 3D CAD system are carried out. There are two types of 3D CAD system. One is manifold modeler, the other is non-manifold modeler. In the manifold modeler only 3D objects can be modeled. In the non-manifold modeler 3D, 2D, 1D, and 0D objects can be modeled in a unified data structure. Recently there are many studies on the non-manifold modeler. Most of them are focused on finding unknown topological entities and representing all kinds of topological entities found. In this paper, efficient data structure is selected. The boundary information on a face and an edge is included in this data structure. The boundary information on a vertex is excluded considering the frequency of usage. Because the disk cycle information is not required in most case of modeling. It is compact. It stores essential non-manifold information such as loop cycle and radial cycle. A suitable Euler-Poincare equation is studied and selected. Using the efficient data structure and the selected Euler-Poincare equation, 18 basic Euler operators are implemented. Several 3D models are created using the implemented modeler. A non-manifold modeling can be carried out using the implemented 3D CAD system. The results of this paper could be used in the further studies such as an implementation of Boolean operators, and a translation of 2D CAD drawings to 3D models.

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A Study on the Physical Environment Characteristics of the Detached Housing Area in the Housing Site Development District (택지개발지구내 단독주택지의 물리적 환경특성에 관한 연구)

  • 박몽섭;하재명
    • Journal of the Korean housing association
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    • v.15 no.3
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    • pp.43-51
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    • 2004
  • The purpose of this study is to examine the physical characteristics of the detached housing area in the housing site development district. The process of this study is to investigate the characteristics of the physical structure and the building use, then to analyze the relationship of physical structure and the building use in the aspect of the morphological characteristics. The case study focuses on the Chil-kok2, An-Siml in Taegu. The streets pattern is composited gird and loop type mixed. The block is composited by 2 lots in general. But the obscurity of organization and character of the streets, the size and shape of lots which is not fit to the building use. A whole residential characters appears high residential density ratio. so residential environment became worse. The improving level of residential environment and efficiency of land use in the detached housing area of the housing site development district, firstly the propriety of the physical structure must be considered about detached house characters, and the housing uses which fit to the physical structure are needed, and also for the concrete realization of this, the designation of district, the zoning of residential area, the guideline of the district design, and establishment of comprehensive master planning in detached housing area are needed in aspect of the housing policy. As the result of this study, it suggests several design guideline to improve housing site development.

Studies on control mechanism and performance of a novel pneumatic-driven active dynamic vibration absorber

  • Kunjie Rong;Xinghua Li;Zheng Lu;Siyuan Wu
    • Structural Engineering and Mechanics
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    • v.87 no.2
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    • pp.117-127
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    • 2023
  • To efficiently attenuate seismic responses of a structure, a novel pneumatic-driven active dynamic vibration absorber (PD-ADVA) is proposed in this study. PD-ADVA aims to realize closed-loop control using a simple and intuitive control algorithm, which takes the structure velocity response as the input signal and then outputs an inverse control force to primary structure. The corresponding active control theory and phase control mechanism of the system are studied by numerical and theoretical methods, the system's control performance and amplitude-frequency characteristics under seismic excitations are explored. The capability of the proposed active control system to cope with frequency-varying random excitation is evaluated by comparing with the optimum tuning TMD. The analysis results show that the control algorithm of PD-ADVA ensures the control force always output to the structure in the opposite direction of the velocity response, indicating that the presented system does not produce a negative effect. The phase difference between the response of uncontrolled and controlled structures is zero, while the phase difference between the control force and the harmonic excitation is π, the theoretical and numerical results demonstrate that PD-ADVA always generates beneficial control effects. The PD-ADVA can effectively mitigate the structural seismic responses, and its control performance is insensitive to amplitude. Compared with the optimum tuning TMD, PD-ADVA has better control performance and higher system stability, and will not have negative effects under seismic wave excitations.

A Novel Design of Frequency Multiplier Using Feedforward Technique and Defected Ground Structure (Feedforward와 Defected Ground Structure를 이용한 주파수 체배기 설계)

  • Park Sang-Keun;Lim Jong-Sik;Jeong Yong-Chae;Kim Chul-Dong
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.17 no.8 s.111
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    • pp.725-731
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    • 2006
  • A novel design of frequency multiplier using a feedforward technique and a defected ground structure(DGS) is proposed. The feedforward loop in the proposed frequency multiplier suppresses the fundamental component $(f_0)$, the dumb-bell or spiral shaped DGS diminish unwanted harmonics such as second, third and fourth. Due to the combination of the feedforward structure and the DGS, only the multiplied frequency component$(2f_0,\;3f_0,\;4f_0)$ appears at the output port and the other unwanted components are suppressed excellently. The frequency multiplier is designed at 1 GHz $(f_0)$, by the proposed technique and measured. The measured output power of $2f_0,\;3f_0$ and $4f_0$ is -2.59 dBm, -5.36dBm and -4.57dBm, respectively, when the input power is 0dBm.